• Title/Summary/Keyword: Bio fabrication

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Fabrication of Multi-layered Macroscopic Hydrogel Scaffold Composed of Multiple Components by Precise Control of UV Energy

  • Roh, Donghyeon;Choi, Woongsun;Kim, Junbeom;Yu, Hyun-Yong;Choi, Nakwon;Cho, Il-Joo
    • BioChip Journal
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    • v.12 no.4
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    • pp.280-286
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    • 2018
  • Hydrogel scaffolds composed of multiple components are promising platform in tissue engineering as a transplantation materials or artificial organs. Here, we present a new fabrication method for implementing multi-layered macroscopic hydrogel scaffold composed of multiple components by controlling height of hydrogel layer through precise control of ultraviolet (UV) energy density. Through the repetition of the photolithography process with energy control, we can form several layers of hydrogel with different height. We characterized UV energy-dependent profiles with single-layered PEGDA posts photocrosslinked by the modular methodology and examined the optical effect on the fabrication of multi-layered, macroscopic hydrogel structure. Finally, we successfully demonstrated the potential applicability of our approach by fabricating various macroscopic hydrogel constructs composed of multiple hydrogel layers.

Effects of core characters and veneering technique on biaxial flexural strength in porcelain fused to metal and porcelain veneered zirconia

  • Oh, Ju-Won;Song, Kwang-Yeob;Ahn, Seung-Geun;Park, Ju-Mi;Lee, Min-Ho;Seo, Jae-Min
    • The Journal of Advanced Prosthodontics
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    • v.7 no.5
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    • pp.349-357
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    • 2015
  • PURPOSE. The purpose of this study was to assess the impact of the core materials, thickness and fabrication methods of veneering porcelain on prosthesis fracture in the porcelain fused to metal and the porcelain veneered zirconia. MATERIALS AND METHODS. Forty nickel-chrome alloy cores and 40 zirconia cores were made. Half of each core group was 0.5 mm-in thickness and the other half was 1.0 mm-in thickness. Thus, there were four groups with 20 cores/group. Each group was divided into two subgroups with two different veneering methods (conventional powder/liquid layering technique and the heat-pressing technique). Tensile strength was measured using the biaxial flexural strength test based on the ISO standard 6872:2008 and Weibull analysis was conducted. Factors influencing fracture strength were analyzed through three-way ANOVA (${\alpha}{\leq}.05$) and the influence of core thickness and veneering method in each core materials was assessed using two-way ANOVA (${\alpha}{\leq}.05$). RESULTS. The biaxial flexural strength test showed that the fabrication method of veneering porcelain has the largest impact on the fracture strength followed by the core thickness and the core material. In the metal groups, both the core thickness and the fabrication method of the veneering porcelain significantly influenced on the fracture strength, while only the fabrication method affected the fracture strength in the zirconia groups. CONCLUSION. The fabrication method is more influential to the strength of a prosthesis compared to the core character determined by material and thickness of the core.

Fabrication and Properties Analysis of MEA for PEMFC (고분자전해질 연료전지용 MEA 제조 및 특성평가)

  • Cho Y.H.;Cho Y.H.;Park I.S.;Sung Y.E.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.230-234
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    • 2005
  • Fabrication of MEA is important factor for proton exchange membrane fuel cell (PEMFC). MEA of PEMFC with hot pressing and direct coating method were prepared, and performances were evaluated and compared each other. The effect of MEA preparation methods, hot pressing methods and direct coating methods, on the cell performance was analyzed by impedance spectroscopy and SEM. The performance of PEMFC with direct coating method was better than with hot pressing method because membrane internal resistance and membrane-interfacial resistance were reduced by elimination of hot pressing process in MEA fabrication. In addition the micro structure of MEA with direct coating method reveals uniform interface between membrane and catalyst layer.

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Fabrication of plastic CE (capillary electrophoresis) microchip by hot embossing process (핫 엠보싱 공정을 이용한 플라스틱 CE(capillary electrophoresis) 마이크로 칩의 제작)

  • Cha Nam-Goo;Park Chang-Hwa;Lim Hyun-Woo;Park Jin-Goo
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1140-1144
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    • 2005
  • A plastic-based CE (capillary electrophoresis) microchip was fabricated by hot embossing process. A Si mold was made by wet etching process and a PMMA wafer was cut off from 1mm thick PMMA sheet. A micro-channel structure on PMMA substrate was produced by hot embossing process using the Si mold and the PMMA wafer. A vacuum assisted thermal bonding procedure was employed to seal an imprinted PMMA wafer and a blank PMMA wafer. The results of microscopic cross sectional images showed dimensions of channels were well preserved during thermal bonding process. In our procedure, the deformation amount of bonding process was below 1%. The entire fabrication process may be very useful for plastic based microchip systems.

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Effects of Fabrication Process Variation on Impedance of Neural Probe Microelectrodes

  • Cho, Il Hwan;Shin, Hyogeun;Lee, Hyunjoo Jenny;Cho, Il-Joo
    • Journal of Electrical Engineering and Technology
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    • v.10 no.3
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    • pp.1138-1143
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    • 2015
  • Effects of fabrication process variations on impedance of microelectrodes integrated on a neural probe were examined through equivalent circuit modeling and SPICE simulation. Process variation and the corresponding range were estimated based on experimental data. The modeling results illustrate that the process variation induced by metal etching process was the dominant factor in impedance variation. We also demonstrate that the effect of process variation is frequency dependent. Another process variation that was examined in this work was the thickness variation induced by deposition process. The modeling results indicate that the effect of thickness variation on impedance is negligible. This work provides a means to predict the variations in impedance values of microelectrodes on neural probe due to different process variations.

Fabrication of a metal-ceramic crown to fit an existing partial removable dental prosthesis using ceramic pressed to metal technique: a clinical report

  • Seo, Jae-Min;Ahn, Seung-Geun
    • The Journal of Advanced Prosthodontics
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    • v.6 no.3
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    • pp.241-244
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    • 2014
  • Fabricating a crown to retrofit an existing abutment tooth for a partial removable dental prosthesis (PRDP) is one of the most time-consuming and labor-intensive clinical procedures. In particular, when the patient is concerned with esthetic aspects of restoration, the task of fabricating becomes more daunting. Many techniques for the fabrication of all-metallic or metal-ceramic crowns have been discussed in the literature. This article was aimed to describe a simple fabrication method in which a retrofitting crown was fabricated for a precise fit using a ceramic-pressed-to-metal system.

Photolithographic Silicon Patterns with Z-DOL (perfluoropolyether, PFPE) Coating as Tribological Surfaces for Miniaturized Devices

  • Singh, R. Arvind;Pham, Duc-Cuong;Yoon, Eui-Sung
    • KSTLE International Journal
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    • v.9 no.1_2
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    • pp.10-12
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    • 2008
  • Silicon micro-patterns were fabricated on Si (100) wafers using photolithography and DRIE (Deep Reactive Ion Etching) fabrication techniques. The patterned shapes included micro-pillars and micro-channels. After the fabrication of the patterns, the patterned surfaces were chemically modified by coating Z-DOL (perfluoropolyether, PFPE) thin films. The surfaces were then evaluated for their micro-friction behavior in comparison with those of bare Si (100) flat, Z-DOL coated Si (100) flat and uncoated Si patterns. Experimental results showed that the chemically treated (Z-DOL coated) patterned surfaces exhibited the lowest values of coefficient of friction when compared to the rest of the test materials. The results indicate that a combination of both the topographical and chemical modification is very effective in reducing the friction property. Combined surface treatments such as these could be useful for tribological applications in miniaturized devices such as Micro/Nano-Electro-Mechanical-Systems (MEMS/NEMS).