• Science of Emotion and Sensibility
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• v.14 no.1
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• pp.157-164
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• 2011

Emotion science is one of the rapidly expanding engineering/scientific disciplines which has a major impact on human society. Such growing interests in emotion science and engineering owe the recent trend that various academic fields are being merged. In this paper we propose the potential importance of the biochip technology in which the human emotion can be precisely measured in real time using body fluids such as blood, saliva and sweat. We firstly and newly name such a biochip an Emotion-On-a-Chip (EOC). EOC consists of biological markers to measure the emotion, electrode to acquire the signal, transducer to transfer the signal and display to show the result. In particular, microfabrication techniques made it possible to construct nano/micron scale sensing parts/chips to accommodate the biological molecules to capture the emotional bio-markers and gave us a new opportunities to investigate the emotion precisely. Future developments in the EOC techniques will be able to help combine the social sciences and natural sciences, and consequently expand the scope of studies.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.2993-2998
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• 2009

An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic foodborne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 ${\times}\;10^3$ cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of IMS was greater than 100-fold, this combination resulted in a detection limit of 54 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than 1 CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours.

• Transactions on Electrical and Electronic Materials
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• v.14 no.5
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• pp.273-277
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• 2013

A unified potentiostat circuit for both $O_2$- and $H_2O_2$- based electrochemical glucose sensors was proposed and its function was verified by circuit simulations and measurement results of a fabricated chip. This circuit consisted of an operational amplifier, a comparator and current mirrors. The proposed circuit was fabricated with a $0.13{\mu}m$ thick oxide CMOS process and an active area of $360{\mu}m{\times}100{\mu}m$. The measurements revealed an input operation range from 0.5 V to 1.6 V in the $H_2O_2$- based bio-sensor and from 1.7 V to 2.6 V in the $O_2$- based bio-sensor with a supply voltage of 3.3 V. The evaluation results showed that the proposed potentiostat circuit is suitable for measuring the electrochemical cell currents of both $O_2$- and $H_2O_2$- based glucose sensors.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.8 s.227
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• pp.1135-1139
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• 2004

This paper presents a microextractor for the separation of DNA molecules by their sizes. The DNA microextractor immobilizes the DNA molecules of specific size in the micropillar array by adjusting the period of the crossed electric field, thus providing a starting-point independent target DNA extraction method without separation process monitoring. The DNA microextractor has been fabricated by a three-mask micromachining process. The velocity of three different DNA molecules has been measured at the electric field of E=5V/0.8cm in the fabricated DNA microextractor, resulting in the reorientation times of $4.80{\pm}0.44sec,\;7.12{\pm}0.75sec$, and $9.88{\pm}0.30sec$ for ${\lambda}$ DNA, micrococcus DNA, and T4 DNA, respectively. T4 DNA is trapped in the micropillar array when the crossed electric field of 5V/0.8cm is applied alternately at a 10 second time interval. The present DNA microextractor filters the DNA in a specific size range by adjusting the magnitude and/or the period of the crossed electric field applied in the micropillar array.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.557-557
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• 2012

Silicatein-${\alpha}$, the enzyme extracted from silica spicules in glass sponges, has been studied extensively in the way of chemistry from 1999, in which the pioneering work by Morse, D. E. - the discovery of the enzymatic hydrolysis in Silicatein-${\alpha}$ - was published. Since its reaction conditions are physiologically favored, synthesis of various materials, such as gallium oxide, zirconium oxide, and silicon oxide, was achieved without any hazardous wastes. Although some groups synthesized oxide films and particles, they have not achieved yet controlled morphogenesis in the reaction conditions mentioned above. With the knowledge of catalytic triad involved in hydrolysis of silicone alkoxide and oligomerization of silicic acid, we designed the novel peptide amphiphiles to not only form self-assembled structure, but also display similar activities to silicatein-${\alpha}$. Designed templates were able to self-assemble into left-handed helices for the peptide amphiphiles with L-form amino acid, catalyzing polycondensation of silicic acids onto the surface of them. It led to the formation of silica helices with 30-50 nm diameters. These results were characterized by various techniques, including SEM, TEM, and STEM. Given the situation that nano-bio-technology, the bio-applicable technology in nanometer scale, has been attracting considerable attention; this result could be applied to the latest applications in biotechnology, such as biosensors, lab-on-a-chip, biocompatible nanodevices.

• Journal of Animal Environmental Science
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• v.13 no.1
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• pp.29-34
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• 2007

This study was conducted to develop a reclaimer of the vehicle disinfector to be used at livestock fm. The reclaimer was mainly consisted of ball-valves, geared motors and one-chip processor, and the purpose of the system was to prevent liquid freezing as well as decrease environmental pollution of antiseptic solution. The properly spraying pressure of the vehicle disinfector was found over 1.96 MPa at 1m of the spraying range. While certain amount of the antiseptic solution remained in the injection-pipes, the spray starting time was found not making any significant effect on the remained amount of the antiseptic solution. The amounts of the antiseptic solution remained in the injection-pipes were 50 ml and 270 ml in average, respectively with and without the use of the reclaimer. The reclaimer was the most effective when the connection of the injection-pipe and sprayer line was located below the side-injection-pipe and then connected to the injection-pipe located at the bottom of vehicles.

• Journal of Pharmacopuncture
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• v.25 no.1
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• pp.52-62
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• 2022

Objectives: Snake venom is a complex mixture of various pharmacologically active substances, such as small proteins, peptides, and organic and mineral components. This paper aims to identify and analyse the proteins in common venomous snakes, such as Gloydius blomhoffii (G. blomhoffii) and Agkistrodon acutus (A. acutus), in Korea. Methods: We used mass spectrometry, electrophoresis, N-terminal sequencing and in-gel digestion to analyse the proteins in these two snake venoms. Results: We identified eight proteins in G. blomhoffii venom and four proteins in A. acutus venom. The proteins detected in G. blomhoffii and A. acutus venoms were phospholipase A₂, snake venom metalloproteinase and cysteine-rich secretory protein. Snake C-type lectin (snaclec) was unique to A. acutus venom. Conclusion: These data will contribute to the current knowledge of proteins present in the venoms of viper snakes and provide useful information for investigating their therapeutic potential.

• Genomics & Informatics
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• v.19 no.1
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• pp.2.1-2.10
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• 2021

BRAF inhibitors (e.g., vemurafenib) are widely used to treat metastatic melanoma with the BRAF V600E mutation. The initial response is often dramatic, but treatment resistance leads to disease progression in the majority of cases. Although secondary mutations in the mitogen-activated protein kinase signaling pathway are known to be responsible for this phenomenon, the molecular mechanisms governing acquired resistance are not known in more than half of patients. Here we report a genome- and transcriptome-wide study investigating the molecular mechanisms of acquired resistance to BRAF inhibitors. A microfluidic chip with a concentration gradient of vemurafenib was utilized to rapidly obtain therapy-resistant clones from two melanoma cell lines with the BRAF V600E mutation (A375 and SK-MEL-28). Exome and transcriptome data were produced from 13 resistant clones and analyzed to identify secondary mutations and gene expression changes. Various mechanisms, including phenotype switching and metabolic reprogramming, have been determined to contribute to resistance development differently for each clone. The roles of microphthalmia-associated transcription factor, the master transcription factor in melanocyte differentiation/dedifferentiation, were highlighted in terms of phenotype switching. Our study provides an omics-based comprehensive overview of the molecular mechanisms governing acquired resistance to BRAF inhibitor therapy.

• Journal of agriculture & life science
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• v.53 no.1
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• pp.117-126
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• 2019

This paper presents an experiment to examine the possibility of treating carcass while making compost with a horizontal cylinder composting equipment. The findings were as follows: as for heavy metal content, zinc content was above the allowed level in some measuring sections of the general operation and the entire measuring sections of the carcass operation, whose copper content was higher than the general operation. The wood chip operation was lower than the allowed level in all the heavy metals and similar or the same as the carcass operation in copper, cadmium and arsenic. Its nickel content was 29.5~63.8%, which was relatively higher than 9.3~18.0% and 15.8~18.0% of the general and carcass operation, respectively. Its chrome content was 14.2~31.9%, which was relatively higher than that of the general and carcass operation. The integrated operation was lower than the allowed level in all the heavy metals. Its copper and zinc content was 34.9~54.5% and 53.1~75.9%, which was similar to 48.9~52.6% and 64.6~85.9%, respectively, in the wood chip operation. Its chrome and nickel content was stabilized while their content was rather high in the wood chip operation. The average and final moisture content of the carcass operation was 60.7% and 49.6%, respectively. Its average moisture content was a little bit higher than the recommended level of 55.0%, but its final moisture content was lower than the recommended level. The average and final moisture content of the integrated operation was 29.2% and 18.6%, respectively, which was the lowest level among the four operation modes. The overall moisture content of the operation modes was under the recommended level of 55.0% with some variance among the modes. The C/N ratio was the lowest in the carcass operation and the highest in the general operation. The average C/N ratio of the operation modes was in the range of 13.7~20.3 with the total average of 18.3, and the modes kept it under the proper level of 30.0.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1381-1386
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• 2014

In spite of variation in coat color, size, and production traits among indigenous Bangladeshi cattle populations, genetic differences among most of the populations have not been investigated or exploited. In this study, we used a high-density bovine single nucleotide polymorphism (SNP) 80K Bead Chip derived from Bos indicus breeds to assess genetic diversity and population structure of 2 Bangladeshi zebu cattle populations (red Chittagong, n = 28 and non-descript deshi, n = 28) and a semi-domesticated population (gayal, n = 17). Overall, 95% and 58% of the total SNPs (69,804) showed polymorphisms in the zebu and gayal populations, respectively. Similarly, the average minor allele frequency value was as high 0.29 in zebu and as low as 0.09 in gayal. The mean expected heterozygosity varied from $0.42{\pm}0.14$ in zebu to $0.148{\pm}0.14$ in gayal with significant heterozygosity deficiency of 0.06 ($F_{IS}$) in the latter. Coancestry estimations revealed that the two zebu populations are weakly differentiated, with over 99% of the total genetic variation retained within populations and less than 1% accounted for between populations. Conversely, strong genetic differentiation ($F_{ST}=0.33$) was observed between zebu and gayal populations. Results of population structure and principal component analyses suggest that gayal is distinct from Bos indicus and that the two zebu populations were weakly structured. This study provides basic information about the genetic diversity and structure of Bangladeshi cattle and the semi-domesticated gayal population that can be used for future appraisal of breed utilization and management strategies.