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http://dx.doi.org/10.3795/KSME-A.2004.28.8.1135

A DNA Microextractor Using Crossed Field Electrophoresis  

Yi Soyeon (한국과학기술원 디지털나노구동연구단)
Seo Kyoung-Sun (한국과학기술원 디지털나노구동연구단)
Cho Young-Ho (한국과학기술원 바이오시스템학과 및 기계공학과 디지털나노구동연구단)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.28, no.8, 2004 , pp. 1135-1139 More about this Journal
Abstract
This paper presents a microextractor for the separation of DNA molecules by their sizes. The DNA microextractor immobilizes the DNA molecules of specific size in the micropillar array by adjusting the period of the crossed electric field, thus providing a starting-point independent target DNA extraction method without separation process monitoring. The DNA microextractor has been fabricated by a three-mask micromachining process. The velocity of three different DNA molecules has been measured at the electric field of E=5V/0.8cm in the fabricated DNA microextractor, resulting in the reorientation times of $4.80{\pm}0.44sec,\;7.12{\pm}0.75sec$, and $9.88{\pm}0.30sec$ for ${\lambda}$ DNA, micrococcus DNA, and T4 DNA, respectively. T4 DNA is trapped in the micropillar array when the crossed electric field of 5V/0.8cm is applied alternately at a 10 second time interval. The present DNA microextractor filters the DNA in a specific size range by adjusting the magnitude and/or the period of the crossed electric field applied in the micropillar array.
Keywords
Bio-Separators; Bio-Extractors; Electrophoresis; Crossed Electric Field; Micropillars; Lab-on-a-chip;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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1 McDonell, M.W, Simon, M.N. and Studier, F.W, 1997, 'Analysis of Restriction Fragments of T7 DNA and Determination of Molecular Weights by Electrophoresis in Neutral and Alkaline Gels,' J. Mol. Biol., Vol. 110, pp. 119-146   DOI
2 Chou, H.P., Spence, C., Scherer, A. and Quake, S., 1999, 'A Microfabricated Device for Sizing and Sorting DNA Molecules,' Proc. Natl. Acad. Sci., Vol. 96, pp. 11-13   DOI
3 Han, J. and Craighead, H.G., 2000, 'Separation of Long DNA Molecules in a Microfabricated Entropic Trap Array,' Science, Vol. 288, pp. 1026-1029   DOI   ScienceOn
4 Akerman, B. and Jonsson, M., 1990, 'Reorientational Dynamics and Mobility of DNA during Pulsed-Field Agarose Gel Electrophoresis,' J. Phys. Chem., Vol. 94, pp. 3828-3838   DOI
5 Viovy, J.L., 1989, 'Reptation-breathing Theory of Pulsed Electrophoresis : Dynamic Regimes, Antiresonance and Symmetry Breakdown Effects,' Electrophoresis, Vol. 10, pp. 429-441   DOI   ScienceOn
6 Ertas, D., 1998, 'Lateral Separation of Macromolecules and Polyelectrolytes in Microlithographic Arrays,' Phys. Rev. Lett., Vol. 80, pp. 1548-1551   DOI   ScienceOn
7 Bakajin, Duke, T.A.J., Tegenfeldt, J., Chou, C., Chan, S.S., Austin R.H. and Cox E.C., 2000, 'Separation of 100-Kilobase DNA Molecules in 10 Seconds,' Anal. Chem., Vol. 73, pp. 6053-6056   DOI   ScienceOn
8 Volkmuth, W.D. and Austin, R.H., 1992, 'DNA Electrophoresis in Microlithographic Arrays,' Nature, Vol. 358, pp. 600-602   DOI   ScienceOn
9 Duke, T.A.J., Austin, R.H., Cox, E.C. and Chan, S.S., 1996, 'Pulsed-field Electrophoresis in Microlithographic Arrays,' Electrophoresis, Vol. 17, pp. 1075-1079   DOI   ScienceOn