• Title/Summary/Keyword: Bio Sample

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Quality Characteristics of Muffins containing Maesangi Powder Abstract (매생이가루 첨가량에 따른 머핀의 품질특성)

  • Seo, Eun-Ok;Kim, Kwang-Oh;Ko, Seong-Hye;Park, Jin-Hee;Han, Eun-Ju;Cha, Kyung-Ok;Ko, Eun-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.3
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    • pp.414-421
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    • 2012
  • Moisture content of muffin tended to increased as increasing mixing rate of maesangi. For crude protein, sample group of 9% replaced by maesangi powder was the highest. For crude fat and crude ash, there was no significant difference among sample groups. The average height of muffin was 4.7 cm, but was no significant difference among sample groups. Volume of muffin has been gradually increased as increasing mixing rate of maesangi powder, but was no significant difference among sample groups. From the SEM (scanning electron microscope) result, thick cell wall and rough blowholes were appeared. L-, a and b-values of muffin was declined as per increase of mixing rate of maesangi powder. Hardness was inclined as per increase of mixing rate of maesangi powder. There were significant differences between sample groups and reference group for adhesiveness, springiness and cohesiveness, but was no significant difference among sample groups. For gumminess, sample group of 9% replaced by maesangi powder was the highest, but there were no significant difference from sample groups of 3% and 6% replaced by maesangi powder. Chewiness was inclined as per increase of mixing rate of maesangi powder, and sample group of 9% replaced by maesangi powder was the highest. From sensory test result, sample group of 9% replaced by maesangi powder was the highest for color, and there were significant differences among sample groups. Sample group of 6% replaced by maesangi powder was the highest for flavor and taste, and there were significant differences among sample groups. Sample groups of 6% and 9% replaced by maesangi powder were the highest for texture. Sample group of 9% replaced by maesangi powder was the highest for overall preference. From all these results, the most suitable one would be the sample group of 9% replaced by maesangi powder.

Morphology Evolution of Poly(L-lactic acid) (PLLA), Poly(ε-caprolactone) (PCL) and Polyethylene Oxide (PEO) Ternary Blend and Their Effects on Mechanical Properties for Bio Scaffold Applications (폴리락틱산, 폴리카프로락톤, 폴리에틸렌 옥사이드 삼성분계 블렌드의 형태학적 변화와 이들이 의료용 스캐폴더의 기계적 특성에 미치는 영향)

  • Ezzati, Peyman;Ghasemi, Ismaeil;Karrabi, Mohammad;Azizi, Hamed;Fortelny, Ivan
    • Polymer(Korea)
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    • v.38 no.4
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    • pp.449-456
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    • 2014
  • Ternary blends of poly(L-lactic acid) (PLLA), poly(${\varepsilon}$-caprolactone) (PCL) and polyethylene oxide (PEO) were produced with different concentrations of components via melt blending. By leaching the PEO from the samples by water, porous materials were obtained with potential application for bio scaffolds. Sample porosity was evaluated by calculating the ratio of porous scaffold density (${\rho}^*$) to the non-porous material density (${\rho}_s$). Highest porosity (51.42%) was related to the samples containing 50 wt%. of PEO. Scanning electron microscopy (SEM) studies showed the best porosity resulted by decreasing PLLA/PCL ratio at constant concentration of PEO. Crystallization behavior of the ternary blend samples was studied using differential scanning calorimetry (DSC). Results revealed that the crystallinity of PLLA was improved by addition of PEO and PCL to the samples. The porosity plays a key role in governing the compression properties. Mechanical properties are presented by Gibson-Ashby model.

A High-Lateral Resolution MALDI Microprobe Imaging Mass Spectrometer Utilizing an Aspherical Singlet Lens

  • Han, Sang Yun;Kim, Hwan Jin;Ha, Tae Kyung
    • Bulletin of the Korean Chemical Society
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    • v.34 no.1
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    • pp.207-210
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    • 2013
  • We report the construction of a MALDI imaging mass spectrometer equipped with a specially designed laser focusing lens, a compact aspherical singlet lens, that obtains a high-lateral imaging resolution in the microprobe mode. The lens is specially designed to focus the ionization laser (${\lambda}$ = 355 nm) down to a $1{\mu}m$ diameter with a long working distance of 34.5 mm. With the lens being perpendicular to the sample surface and sharing the optical axis with the ion path, the imaging mass spectrometer achieved an imaging resolution of as good as $5{\mu}m$ along with a high detection sensitivity of 100 fmol for peptides. The mass resolution was about 900 (m/${\Delta}m$) in the linear TOF mode. The high-resolution capability of this instrument will provide a new research opportunity for label-free imaging studies of various samples including tissues and biochips, even for the study at a single cell level in the future.

Method Development and Analysis of Carotenoid Compositions in Various Tomatoes (토마토 종류에 따른 카로티노이드 함량 비교와 다중분석법 개발)

  • Kim, Han-Kyul;Chun, Jin-Hyuk;Kim, Sun-Ju
    • Korean Journal of Environmental Agriculture
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    • v.34 no.3
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    • pp.196-203
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    • 2015
  • BACKGROUND: Purpose of this research is HPLC analysis method development of lycopene in tomato. And then, three components of carotenoid in four kinds of tomatoes (general tomato, cherry tomato, red and orange date tomato) were compared with each other. METHODS AND RESULTS: Lycopene in tomato was extracted with hexane likes other carotenoid components using 500 mg of dried powder sample. HPLC analysis conditions were column temperature ($40^{\circ}C$), detection wavelength (454 nm), flow rate (1.0 mL/min) and injection volume ($20.0{\mu}L$). Lycopene was analyzed by the gradient elution ($60{\rightarrow}100%$) of the mobile phase solvents A[water: methanol=25: 75 (v/v)] and B[ethyl acetate]. CONCLUSION: Three components of carotenoids (lutein, ${\beta}$-carotene, lycopene) were observed in tomatoes. The total carotenoid contents was the highest in red date tomato (662.0 mg/kg dry wt.) and the lowest in orange date tomato (111.3 mg/kg dry wt.). Lycopene contents in tomatoes was the highest percentage (93%) among all the carotenoids.

Use of Calcium Carbonate for Improving Solid Content of KOCC Wet Web (탄산칼슘 적용에 의한 KOCC 지필의 고형분 증대)

  • Hwang, In-Young;Ji, Sung-Gil;Seo, Yung-Bum
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.45 no.6
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    • pp.1-9
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    • 2013
  • For the manufacture of linerboard with 100% KOCC, we tried to increase the solid content of wet web by employing GCC (grounded calcium carbonate) in the fiber furnish to save drying energy. Three different diameters of GCC, namely, 5, 10, and $35{\mu}m$, were used. To complement the strength loss by the addition of GCC, cationic starch and refining treatment were tried. It was found that the addition of $10-35{\mu}m$ dia. GCC to KOCC for $180g/m^2$ basis weight sheets increased the solid content of the furnish about 1-1.5% with better bulk and drainage properties. The loss of strength properties were compensated by the application of cationic starch and/or refining process to the KOCC furnish. The dia. of GCC of $35{\mu}m$ was, however, too large to make smooth surface of the sample sheet. So, the optimization process was required before implementing the results to the mill by selecting proper diameter and shape of the calcium carbonate.

Inertial Microfluidics-Based Cell Sorting

  • Kim, Ga-Yeong;Han, Jong-In;Park, Je-Kyun
    • BioChip Journal
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    • v.12 no.4
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    • pp.257-267
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    • 2018
  • Inertial microfluidics has attracted significant attention in recent years due to its superior benefits of high throughput, precise control, simplicity, and low cost. Many inertial microfluidic applications have been demonstrated for physiological sample processing, clinical diagnostics, and environmental monitoring and cleanup. In this review, we discuss the fundamental mechanisms and principles of inertial migration and Dean flow, which are the basis of inertial microfluidics, and provide basic scaling laws for designing the inertial microfluidic devices. This will allow end-users with diverse backgrounds to more easily take advantage of the inertial microfluidic technologies in a wide range of applications. A variety of recent applications are also classified according to the structure of the microchannel: straight channels and curved channels. Finally, several future perspectives of employing fluid inertia in microfluidic-based cell sorting are discussed. Inertial microfluidics is still expected to be promising in the near future with more novel designs using various shapes of cross section, sheath flows with different viscosities, or technologies that target micron and submicron bioparticles.

Comparison Study of Thermal Decomposition Characteristics of Wattle & Pine Tannin-based Adhesives

  • Kim, Sumin;Lee, Young-kyu;Kim, Hyun-Joong;Eom, Young Geun
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.3
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    • pp.34-41
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    • 2002
  • This study investigated the thermogravimetric analysis of two types of cured tannin-based adhesives from wattle and pine, with three hardeners of paraformaldehyde, hexamethylenetetramine and TN (tris(hydroxyl)nitromethan), at a temperature of 170℃ and a heating rate of 5, 10, 20 and 40℃/min for 10 minutes. The 5 minutes cured wattle tannin-based adhesive with each hardener at 170℃ was also tested to compare the degree of curing. It was found that thermogravimetric analysis could be used to measure the degree of curing of a thermosetting adhesive. The TG-DTG curves of all the adhesive systems were similar and showed three steps in a similar way to a phenolic resin. This means that each adhesive system is well cross-linked. However, a high thermal decomposition rate was shown at 150 to 400℃ in the case of the pine tannin sample with TN (tris(hydroxyl)nitromethan). The Flynn & Wall expression was used to evaluate the activation energy for thermal decomposition. As the level of conversion (𝛼) increased, the activation energy of each system increased. The activation energy of the wattle tannin-based adhesive with paraformaldehyde was higher than the others.

An efficient microscopic technique for aleurone observation with an entire kernel cross-section in maize (Zea mays L.)

  • Jae-Hong Kim;Ji Won Kim;Gibum Yi
    • Korean Journal of Agricultural Science
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    • v.50 no.4
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    • pp.645-652
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    • 2023
  • The aleurone layer in maize is crucial as it contains essential nutrients such as minerals, vitamins, and high-quality proteins. While most of the maize varieties are known to possess a single aleurone layer, several multi-aleurone layer mutants and landraces have been suggested for hierarchical genetic control of aleurone development. Conventional microscopy analysis often involves using immature seeds or sampling only a portion of the kernel sample, and whole kernel section analysis using a microtome is technically difficult and time-consuming. Additionally, the larger size of maize kernels posed challenges for comprehensive cross-sectional analysis compared to other cereal crops. Consequently, this study aimed to develop an efficient method to comprehensively understand the aleurone layer characteristics of the entire cross-section in maize. Through observations of diverse maize genetic resources, we confirmed irregular aleurone layer patterns in those with multiple aleurone layers, and we discovered a landrace having multiple aleurone layers. By selectively identifying genetic resources with multiple aleurone layers, this method may contribute to efficient breeding processes in maize.

Development of a Test Strip Reader for a Lateral Flow Membrane-based Immunochromatographic Assay

  • Park, Je-Kyun;Kim, Suhyeon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.2
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    • pp.127-131
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    • 2004
  • A low-cost, simple strip reader system using a linear movement mechanism of CD-ROM deck has been developed to characterize a lateral flow membrane-based immunochromatographic assay. The test strip reader was assembled by a CD-ROM deck and home-made optical head especially designed for immunoassays. The optical head for detecting reflected light from the test strip surface consists of green light-emitting diode, large area silicon photodiode, and anodized aluminum mounting block providing a slit structure for cutting light from the LED. The stepping motor of the deck was operated in the full step mode, whose distance of each reading point is about 0.15mm. The performance of the strip reader was tested by analysis of HBV(hepatitis B virus) antigen test kit. This strip reader can be useful for inexpensive, disposable, and membrane-based assays that provide visual evidence of the presence of an analyte in a liquid sample.

Microfluidic Devices for Cell Analysis

  • Bachman, Mark;Li, G.P.
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.11a
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    • pp.3.2-3.2
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    • 2009
  • Microfluidics and BioMEMStechnology has increasingly been used as a tool for studying small volumes oftissue and even individual cells. One of the most important benefits ofmicrofluidic technology is the potential to build devices that analyze and sortmammalian cells. The "sorting problem" typically requires that a fewcells be selected and isolated from a larger population of hundreds, thousandsor even millions of other cells. For example, cancer tumor cells may resideamong a large population of healthy cells, but it would be of great interest toidentify, isolate and study only the cancer cells. In another application, onemay want to determine the number of white blood cells within a sample of blood.We have developed microfluidic devices that enable researchers to select cellsfrom a population by a variety of methods, including antibody staining,dielectrophoretic selection, and physical size selection. These devices haveapplications in cancer research where cancer cells must be identified fromnormal tissue, but where only small samples of tissue are available. In thistalk, we will present some of our microfluidic cell sorting devices, discusstheir physical principles, and their use in biological applications.

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