• 제목/요약/키워드: Bio Sample

검색결과 397건 처리시간 0.031초

입경 분류된 토양의 RGB 영상 분석 및 딥러닝 기법을 활용한 AI 모델 개발 (Development of Deep Learning AI Model and RGB Imagery Analysis Using Pre-sieved Soil)

  • 김동석;송지수;정은지;황현정;박재성
    • 한국농공학회논문집
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    • 제66권4호
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    • pp.27-39
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    • 2024
  • Soil texture is determined by the proportions of sand, silt, and clay within the soil, which influence characteristics such as porosity, water retention capacity, electrical conductivity (EC), and pH. Traditional classification of soil texture requires significant sample preparation including oven drying to remove organic matter and moisture, a process that is both time-consuming and costly. This study aims to explore an alternative method by developing an AI model capable of predicting soil texture from images of pre-sorted soil samples using computer vision and deep learning technologies. Soil samples collected from agricultural fields were pre-processed using sieve analysis and the images of each sample were acquired in a controlled studio environment using a smartphone camera. Color distribution ratios based on RGB values of the images were analyzed using the OpenCV library in Python. A convolutional neural network (CNN) model, built on PyTorch, was enhanced using Digital Image Processing (DIP) techniques and then trained across nine distinct conditions to evaluate its robustness and accuracy. The model has achieved an accuracy of over 80% in classifying the images of pre-sorted soil samples, as validated by the components of the confusion matrix and measurements of the F1 score, demonstrating its potential to replace traditional experimental methods for soil texture classification. By utilizing an easily accessible tool, significant time and cost savings can be expected compared to traditional methods.

Distribution Characteristics of Heavy Metals in Soil in the Vicinity of King Sejong Station, King George Island, Antarctica

  • Choi, Ik-Won;Seo, Dong-Chul;Kang, Se-Won;Lee, Sang-Gyu;Seo, Young-Jin;Han, Jong-Hak;Heo, Jong-Soo;Cho, Ju-Sik
    • 한국토양비료학회지
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    • 제45권1호
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    • pp.103-106
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    • 2012
  • To investigate distribution characteristics of heavy metals in soils in the vicinity of King Sejong Station of King George Island, Antarctica, Fe, Mn, Cu, Zn, Pb, Co, Cd, Cr, Ni and Mo contents were investigated under different distances and directions from King Sejong Station. 13 sample sites were distributed from King Sejong Station. In site 4, the Cd content was higher than that in other sites. The Cr content was the highest in site 9, and the Ni and Mn contents were high in site 12. Cu, Co and Zn were highly contained in distance 1~2 km from King Sejong Station. Cd, Cr, Ni and Mo contents were lower, whereas Fe, Mn, Cu, Zn, Pb and Co contents were higher than other heavy metal contents at all sites.

고효율 크기 가변적 입자 분리를 위한 통합 하이브리드 소자 (Integrated Hybrid Device for High-Efficiency Size-Tunable Particle Separation)

  • 추승희;박지온;김태은;강태경;안준석;오가영;김여진;박규빈;박채원;이민정;임현정;남정훈
    • 대한의용생체공학회:의공학회지
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    • 제43권3호
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    • pp.170-176
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    • 2022
  • Cell separation from a heterogenous mixture sample is an essential process for downstream analysis in biological, chemical, and clinical applications. This study demonstrates an integrated hybrid device of the viscoelastic focusing in a straight rectangular channel and subsequent size-based separation using acoustophoresis to attain high efficiency and separation tunability. For particle pre-alignment in a viscoelastic fluid, the flow rate higher than 10 μl/min was required. Surface acoustic wave-based lateral migration of particles with different sizes (13 and 27 ㎛) was examined at various applied voltages and flow rate conditions. Therefore, the flow rate of 100 μl/min and the applied voltage of 20 Vpp can be used for size-based particle separation.

나노임프린팅 기술을 이용한 유연성 브래그 반사 광도파로 소자 (Bragg Reflecting Waveguide Device Fabricated on a Flexible Substrate using a Nano-imprinting Technology)

  • 김경조;이정아;오민철
    • 한국광학회지
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    • 제18권2호
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    • pp.149-154
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    • 2007
  • 저가의 소자 개발이 가능한 나노임프린팅 공정을 도입하여 510 nm 주기의 브래그 격자 구조를 가지는 폴리머 광도파로 소자를 제작하였다. 폴리머 격자 광소자의 온도 의존성을 감소시키기 위한 방법으로 플라스틱 박막으로 이루어진 유연성 기판상에 브래그 격자를 제작하는 것이 필요하다. 임프린팅 공정을 손쉽게 수행하기 위한 광도파로 구조를 채택하였으며, 코아와 클래딩의 굴절률이 각각 1.540, 1.430인 폴리머를 이용하여 코아 두께가 $3{\mu}m$인 단일모드 광도파로 구조를 얻을 수 있었다. 유연성 기판 브래그 격자 광도파로 소자의 특성을 Si기판 브래그 격자 광도파로 소자와 비교하여 관측한 결과, 유연성 기판 도입에 따른 브래그 반사 소자의 성능 저하는 나타나지 않았다.

A Study on Microbial Contamination of Foods Exposed to Multiple Environments

  • KIM, Dan-Bee;CHA, Seong-Soo
    • 식품보건융합연구
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    • 제5권3호
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    • pp.35-40
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    • 2019
  • In this study, general bacterial counts and coliform counts, which are hygienic indicator microorganisms, were tested for candy, chocolate, and jelly which are easily available and enjoyed around. After dropping each sample on the desk, indoors, and outdoors, it is immediately collected, or washed and collected to confirm the myth of the 3-second rule. Immediately after removing the wrapping paper, each sample was dropped on the desk, indoors, and outdoors, and after 3 seconds from the moment of contact with the surface, and then collected in a sample bag using sterilized sanitary gloves. After the same operation, each sample was rinsed for 5 seconds using sterilized sanitary gloves and sterilized distilled water, and then collected in a sample bag. The number of bacteria detected in non-washing candies was 41 CFU/g at outdoor and the number of bacteria detected in non-washing chocolate was 76 CFU/g at outdoor. The number of bacteria detected in non-washing jellies was 79 CFU/g at outdoor. Coliform group was not detected in all samples. This showed good results at the level of m = 10,000 or less, which is an allowable value suggested in the Food Code. Also, effect of washing on contaminated food was confirmed. This result is remarkably low compared with the microorganism specimens shown in Food Code, and it is confirmed that contamination occurs but not high value. Therefore, the myth of the 3-second rule is true compared to the figures based on Food Code. However, it showed the characteristics of bacteria that could survive and cross-contaminate on dry food surfaces and emphasized the importance of hygiene through food contact to unsanitary surfaces to minimize the risk of food poisoning.

반응표면분석법을 이용한 퉁퉁마디 에탄올 추출조건의 최적화 (Optimization of Ethanol Extraction Conditions from Glasswort (Salicornia herbacea) Using Response Surface Methodology)

  • 박정욱;김해섭;박인배;신궁원;이영재;조영철
    • 한국식품저장유통학회지
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    • 제16권3호
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    • pp.376-384
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    • 2009
  • 본 실험은 퉁퉁마디의 에탄올 추출조건에 따른 당도, 수율, 전자공여작용 및 총 페놀화합물 함량에 대해 반응표면 분석법을 이용하여 추출조건을 최적화하였다. 중심합성계획에 따라 용매에 대한 시료비($1{\sim}9\;g$/100 mL), 추출온도($35{\sim}95^{\circ}C$) 및 에탄올 농도($0{\sim}100%$)를 달리하였을 때 반응표면 회귀식의 R2는 당도, 수율, 전자공여작용 및 총 페놀화합물 함량에서 각각 0.7866(p<0.1), 0.8370(p<0.05), 0.9037(p<0.01) 및 0.9263(p<0.01)로 나타났다. 추출조건별 당도, 수율, 전자공여작용 및 총 페놀화합물 함량에 대한 반응표면을 superimposing하여 얻은 최적 추출조건 범위는 용매에 대한 시료비 $3.38{\sim}5.33\;g$/100 mL, 추출온도 $55.87{\sim}76.96^{\circ}C$, 에탄올 농도 $25{\sim}67.31%$로 나타났다. 최적 추출조건범위내의 임의의 조건인 용매에 대한 시료비 5 g/100 mL, 추출온도 $65^{\circ}C$ 및 에탄올 농도 60%를 회귀식에 대입하여 얻은 예측값은 당도 $6.36^{\circ}Brix$, 염도 5.08%, 수율 35.40%, 전자공여작용 61.24% 및 총 페놀성 화합물 917.22 mg/100 g으로 예측되었으며, 실제 실험을 통해 얻어진 값은 당도 $6.50^{\circ}Brix$, 염도 5.10%, 수율 33.84%, 전자공여작용 63.23% 및 총 페놀성 화합물 921.42 mg/100 g으로 매우 유사하게 나타났다.

재배열 항원 수용체 유전자 증폭을 통한 개 림프종의 진단 (Detection of Canine Lymphoma by the Amplification of Antigen Receptor Gene Rearrangements)

  • 유도현;이영화;이종현;노동호;송루희;이미진;최을수;박진호
    • 한국임상수의학회지
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    • 제26권5호
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    • pp.419-422
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    • 2009
  • 개의 림프종을 분자유전자학적으로 진단하고자 본 연구를 수행하였다. 이를 위하여 12개의 염색 및 고정된 도말 표본으로부터 DNA를 추출한 후, 임파육종에 특이적인 재배열된 항원 수용체 유전자를 중합효소연쇄반응으로 증폭시켰다. 그 결과 6개의 type-B 림프종에서 type-B 세포의 림프종에 특이적인 IgH (immunoglobulin H) 주유전자(major gene)와 type-B 림프종의 부유전자(minor gene)가 증폭되었다. 또한, 3개의 type-T 세포의 림프종의 경우에도 이에 특이적인 $TCR{\gamma}$ 유전자가 증폭되었다. 한편, 에를리키아증에 이환된 표본은 $TCR{\gamma}$ 유전자가 위양성으로 증폭되었지만, 활성화된 림프절 샘플의 경우에는 어떤 유전자의 재배열도 관찰되지 않았다. 따라서 림프종의 진단에 있어서 혈액 또는 조직 도말 표본의 DNA를 이용하여 재배열된 항원 수용체 유전자의 검출을 시도한다면, 특이적이고 객관적으로 림프종을 감별진단 할 수 있을 것이며, 나아가 회고적 분석에도 유용하게 활용될 수 있을 것으로 사료된다.

바이오칩 제작을 위한 DNA 시료 조작 정밀 로봇 시스템 개발 (V) - DNA 시료의 실리콘 웨이퍼에의 점착 성능에 관한 연구 - (- Development of Precise Robot System Operating DNA Sample for Manufacturing Bio chips (V) - A Study of Performance Test for DNA Sample Spotting on Silicon Wafer -)

  • 엄기남;김찬수;이영규;김기대
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 2005년도 하계 학술대회 논문집
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    • pp.337-342
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    • 2005
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열수추출 및 효소처리에 따른 감귤 과피 추출물의 기능성성분 수득에 대한 연구 (A Study on the Yield of Functional Components of Citrus Peel Extracts using Optimized Hot Water Extraction and Enzymatic Hydrolysis)

  • 노정은;윤성란;임애경;김혜정;허담;김대익
    • 한국식품조리과학회지
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    • 제28권1호
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    • pp.51-55
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    • 2012
  • This study was conducted to investigate the physicochemical properties of citrus peel extracts with different hot water extraction and enzymatic hydrolysis conditions. Enzymatic hydrolysis was also employed using Viscozyme L and results were compared with that of optimized hot water extract. Hot water extraction was performed under different parameters; the sample to solvent ratio(1:20, 1:15, 1:10), extraction time(2, 4 hrs), extraction temperature(85, $95^{\circ}C$) and enzymatic hydrolysis(0, 1%) and the subsequent extracts were used for determining their physicochemical properties, such as total yield, total phenolics, total flavonoids, and electron donating ability (EDA). With the increase in the sample to solvent ratio and extraction time, total yield, total phenolics, total flavonoids and EDA increased. But extraction temperature did not significantly affect the hot water extract. As hot water extract was hydrolyzed by the enzyme, total yield and active ingredients increased rapidly. In the result of total yield, total phenolics, total flavonoids and EDA, the activity of enzyme-treated extract was higher than those of enzyme-untreated extract. Based upon the overall hot water extraction efficiency, it was found that 20 times volume or 120 min at a time at $95^{\circ}C$ after enzyme treatment was optimal.