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Optimization of Ethanol Extraction Conditions from Glasswort (Salicornia herbacea) Using Response Surface Methodology  

Park, Jeong-Wook (Jeollanam-do Marine Bio Research Institute)
Kim, Hae-Seop (Jeollanam-do Marine Bio Research Institute)
Park, In-Bae (Jeollanam-do Marine Bio Research Institute)
Shin, Gung-Won (Jeollanam-do Marine Bio Research Institute)
Lee, Young-Jae (Jeollanam-do Marine Bio Research Institute)
Jo, Yeong-Cheol (Jeollanam-do Marine Bio Research Institute)
Publication Information
Food Science and Preservation / v.16, no.3, 2009 , pp. 376-384 More about this Journal
Abstract
Response surface methodology (RSM) was used to monitor the characteristics of ethanol extracts from glasswort (Salicornia herbacea). A central composite design was used to investigate the effects of the independent variables of sample ratio, extraction temperature, and ethanol concentration on the dependent variables color, sugar, salinity, yield, electron donating ability, and total polyphenol content of extracts. The maximum $^{\circ}Brix$ (8.46) was obtained under specific extraction conditions, with a sample ratio of 7.04 g/100 mL, an extraction temperature of $89.01^{\circ}C$, and an ethanol concentration of 34.29% v/v. At a sample ratio, extraction temperature, and ethanol concentration of 7.00 g/100 mL, $89.15^{\circ}C$, and 34.14% v/v, respectively, the salinity was 7.35%. When the sample ratio, extraction temperature, and ethanol concentration were 5.56 g/100 mL, $68.61^{\circ}C$, and 99.14% v/v, respectively, the maximum electron donating ability was 86.10%. A maximized total polyphenol content of 1,140.15 mg/100 g was found with the following conditions: sample ratio of 8.6 g/100 mL, extraction temperature of $64.19^{\circ}C$, and ethanol concentration of 71.74% v/v. Overall, the optimal ranges of extraction conditions for effective components of glasswort were 3.38.5.33 g/100 mL sample ratio, $55.87-76.96^{\circ}C$, and 25.00.67.31% v/v ethanol.
Keywords
glasswort; Salicornia herbacea; response surface methodology; optimization;
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