• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.509-522
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• 2008

한국산 가막사리속(Bidens L.)에 포함된 10분류군(8종 2변종)의 수과형태를 연구하기 위하여 입체현미경과 주사전자현미경을 이용하여 관찰하였다. 연구된 가막사리속에서 까끄라기를 제외한 수과의 크기는 $3.0-19.5{\times}0.7-2.6 mm$이다. 관모는2-4개의 까끄라기로 구성되어 있고, 까끄라기의 길이는 2.2-5.6 mm이다. 강모의 배열은 1-3열이었다. 수과의 다형화가 6개의 분류군(B. bipinnata, B. biternata, B. frondosa, B. parviflora, B. pilosa var. pilosa, B. tripartita var. tripartita)에서 확인되었다. 수과의 형태와 까끄라기의 수에 의해 4가지 유형으로 구분되었다. - Type A: 장타원형이고, 까끄라기 수는 2개(B. parviflora); Type B: 장타원형이고, 까끄라기 수는 3개 이상(B. bipinnata, B. biternata, B. pilosa var. pilosa, B. pilosa var. minor) Type C: 도란형이고, 까끄라기의 수는 2개(B. frondosa, B. radiata var. radiata, B. radiata var. pinnatifida, B. tripartita var. tripartita); Type D: 도란형이고, 까끄라기의 수는 3개 이상(B. cernua). 까끄라기의 강모 표면무늬는 3가지 즉 나선상, 평활상, 불규칙한 직선형으로 나타났다. 수과의 표면은 각피층이 발달한 다각형, 평활상 그리고 굴곡형으로 나타났다. 수과형질의 분류학적 적용에 대하여 간략하게 토의하였다. 수과형질을 바탕으로 한국산 가막사리속 분류군에 대한 검색표를 제시하였다.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.53-57
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• 1999

The experiments were conducted to determine the factors such as light and darkness, phytohormone and seed coat, influencing on seed germination of Bidens tripartita L. and B. frondosa. The seeds of both species were germinated when seed coat was damaged and weakened. $GA_3$ and BA stimulated germination of both species but ABA and IAA had no effect on germination of them, which ranged 50.0% to 80.0%. In B. forndosa, when inner layer of seed coat was removed, germination was highly promoted up to 96.7% compared with 10.0% germination rate in another treatments.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.101-110
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• 2004

The growth and niche breath of four species, i.e., native plants such as Bidens tripauita and B. bipinnata and naturalized plants such as B. frondosa and B. pilosa var. minor, were studied as related with environmental gradients including light intensity, soil moisture and soil nutrient. There were no significant differences in the phonology within each environmental gradients while considerable differences were found between species; the two naturalized species bloomed and produced fruit later than the two native species. Two naturalized species exhibited relatively higher total dry weight than the two native counterparts within all environmental gradients. Total dry weight showed positive responses to light intensity and soil moisture, and negatively to soil nutrient. The relative contribution of the environmental factors to total dry weight decreased in the order of soil nutrient, soil moisture and light intensity. Both B. bipinnata and B. pilosa var. minor showed significantly higher net assimilation rate (NAR) than other species. Also, NARs of B. bipinnata and B. pilosa var. minor decreased with increasing soil nutrient. Relative growth rate (RGR) decreased in order of B. tripartita, B. bipinnata, B. frondosa and B. pilosa var. minor in response to light intensity. In addition, RGRs of B. bipinnata and B. piEosa yay. minor increased in response to soil moisture, while those of B. pilosa var. minor and B. tripartita decreased with increment of soil nutrient. No significant fluctuations of shoot/root ratio were not observed in three species, but a native species, B. tripartita showed n decreased shoot/root ratio in response to soil nutrient. Comparing the growth characteristics of the species, B. pitosa var. minor and 3. tripartita revealed vigorous growth on barren soil. On the other hand, B. frondosa exhibited vigorous growth on fertile soil. Morphologically, B. tripartita adapted to light and required considerable moisture. On the contrary, the leaves of B. bipinnata did not change considerably in their area, although it preferred habitat with abundant light. In terms of niche breadth, B. bipinnata showed the widest ranges of 0.875 and 0.845 for light intensity and soil moisture gradients, respectively. B. pilosa var. minor showed a value of 0.933 for soil nutrient gradient. B. tripartita showed narrow ranges for the three environmental factors, whereas B. frondosa showed wide values for light intensity and soil nutrient, but relatively narrow value for soil moisture.

• Korean Journal of Weed Science
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• v.17 no.3
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• pp.303-309
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• 1997

Variation of morphological and physiological traits of 50 Bidens tripartita accessions were studied and the accessions were grouped through cluster analysis based on four major characters; plant type, leaf partition, achene length, days to flowering. Bidens tripartite accessions have shown significant variations in plant type, stem length, days to flowering, leaf shape, leaf partition, chlorophyll content, leaf color, stem color, achene color, achene length and achene shape. Most of Bidens tripartite accessions appeared to have strong dormancy and also photodormancy with some exceptions. Plants could be classified into 5 types from straight(I) to triangle(V), and intermediate diamond type(III) was prevalent. The plant type score has negative correlation with the stem length. None, three, and five part leaved plants were observed and most of them were three or five parted. Leaf partition had negative correlation with achene length and chlorophyll content. Average days to flowering was 108 days in the range of 94~141 days. It had positive correlation with achene length and leaf shape and negative correlation with achene color. Average achene length was 10.0mm and it had positive correlation with achene shape, stem length, days to flowering and leaf shape. It also had negative correlation with leaf color, stem color, achene color, leaf partition. Bidens tripartite accessions could be divided into identifiable six groups from the cluster analysis at the distance 0.06 using Ward's minimum-variance method.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.195-203
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• 2012

The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil：17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

• Korean Journal of Weed Science
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• v.16 no.2
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• pp.114-121
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• 1996

This study was conducted to investigate seed germination, seedling growth, and responses to herbicides of Bidens tripartita L. When the field-collected seeds were stored under a dry-room temperature, dry-low temperature, wet-low temperature, or dry-high temperature condition, no seeds were germinated in a growth chamber with 14 hr photoperiod up to 35 days after the storage. Exceptionally, however, some seeds stored under a wet-room temperature condition were germinated after 25 days of the storage. This might be due to the fact that the seed coats were damaged by fungi which developed during the storage. Seeds stored under a wet-low temperature condition (stratification) began to be germinated after 3 months of the storage and the germination rate increased with a prolonged stratification. Almost all seeds were germinated after 9 months of the stratification. These results suggest that the dormancy of B. tripartita L. seeds relate to the seed coat and thus several attempts were made to induce seed germination through damaging or weakening the seed coat. Freezing($-20^{\circ}C$), drying($100^{\circ}C$), or swelling($40^{\circ}C$) of the seeds was not effective to induce the germination. Treatments of concentrated sulfuric acid, $KNO_3$, or gibberellin to the seeds had no effect on inducing the germination. However, ethrel had a stimulatory effect on the germination of the seeds with an optimum concentration of 250ppm. A seed cutting was also effective to induce the germination, but seedlings from the seeds had cutted cotyledons. Germination of the stratified seeds varied with the temperature condition to which they were subjected, but not with light. The germination rate was the highest at 35 - $40^{\circ}C$. Although the seeds were not able to germinate under a submerged condition, seedlings after 2-leaf-stage exhibited better growth under a submerged or a subirrigated condition than under an upland condition. Among the herbicides tested, pyrazosulfuton-ethyl, linuron, and bentazone were found to be effective for controlling B. tripartita L., having more herbicidal effect with an earlier application.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.8-13
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• 1996

This study was conducted to know the effect of storage period, harvesting time and light condition on dormancy breaking of major weeds. Echinochloa crus-galli, Cyperus serotinus, Aeschynomene indica, Ludwigia prostrata and Bidens tripartita had the 10 or 30% germination ability within 30 days after harvest but scirpus juncoides, Potamogeton distinctus, Aneilema japonica and Monochoria vaginalis had not germination untill 120 days after harvest of seed. C. serotinus and P. hydropiper required strong light for germination but S. juncoides, L. prostrata and B. tripartita showed the less tham 5% germination in the dark condition gerntinator. S. juncoides germination showed negative response in chemical soaking but A. japonica, B. tripartita and L. prostrata showed high germination percentage in Acetone, Chloroform and distilled water soaking during the 10 days after treatment.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.429-436
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• 2010

Herbicidal activity and characteristics of chrysophanic acid were investigated in semi-field condition. At early and middle post-emergence, Trifolium repens appeared to be very susceptible to chrysophanic acid of $2,000{\mu}g\;mL^{-1}$. However, herbicidal activity of chrysophanic acid of $2,000{\mu}g\;mL^{-1}$ estimated by visual injury to Artemisia princeps was not caused considerable phytotoxicity. Also by foliar application, the concentration of crysophanic acid for effectively control to Polygonum aviculare was much higher than $2,000{\mu}g\;mL^{-1}$. Herbicidal activity of chrysophanic acid to Echinochloa crus-galli, Cypres difformis, Setaria viridis, Digitaria sangguinalis, Bidens tripartita by foliar application was more effective at concentration ranges from 4,000 to $6,000{\mu}g\;mL^{-1}$. These results suggest that chrysophanic acid demanded for higher than $2,000{\mu}g\;mL^{-1}$ to successful weed control in the field condition.

• Korean Journal of Environmental Biology
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• v.17 no.4
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• pp.407-414
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• 1999

Distribution of biota and removal efficiency of organic matter in natural wetland systems were examined from June to October 1998. The aquatic macrophyte communities were consisted of 13 families and 22 species. The composition of occurrence species were as follows: Phragmites communis Trinius are 35%, Persicaria thunbergii Nakai are 19%, Typha angustifolia L. are 17%, Bidens tripartita L. are 16%, Echinochloa crus-galli (L.) Beauv. are 12% and others are l％. The fauna collected from 5 sites consisted of total 6 classes of macroinvertebrates. The composition of occurrence classes were as follows: Insecta are 76%, O1igochaeta are 19%, Gastrapoda are 4％ and others are 1%. These included 18 families, 7 orders of Insects. The larve and mosquito and midges were found in wetlands. Preventive strategies are needed for the suppression of mosquito at the stage of wetland planning and design. In wetland, removal efficiency of dissolved organic carbon is low because of primary production and the background level of DOC in the wetland discharge seems to be 5 mgC/1, but those of BOD was ca. 50%. Wetlands receiving water of low concentration can not work as purification field.