• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.978-984
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• 2004

In the course of screening for a novel inhibitor of CDC2, HY558-1 was isolated from a culture broth of Penicillium minioluteum F558. Moreover, it was found that HY558-1 had an effect on both the cell cycle regulation and apoptosis of human cervical adenocarcinoma HeLa cells. A flow cytometric analysis of HeLa cells revealed appreciable cell cycle arrest at the G1 and G2/M phases following treatment with HY558-1. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with HY558-1. To obtain further information on the cell cycle arrest and apoptotic induction induced by HY558-1, the expression of certain cell cycle and apoptosis-associated proteins was examined using a Western blot analysis. The results revealed that HY558-1 inhibited the phosphorylation of pRb and decreased the expression levels of CDK2, CDC2, and cyclin A in the cell cycle progression. It was also shown that the level of $p21^{WAF1/CIP1}$ was increased in HeLa cells treated with 0.52 mM of HY558-1. Accordingly, HY558-1 was found to inhibit the proliferation of HeLa cells through the induction of G1 phase arrest by inhibiting pRb phosphorylation via an upregulation of $p21^{WAF1/CIP1}$, and G2/M phase arrest by directly inhibiting CDC2 and cyclin A. Moreover, HeLa cells treated with 0.52 mM of HY558-1 exhibited apoptotic induction associated with the cleavage of Bid and release of cytochrome c from mitochondria into the cytosol. Subsequent investigation of the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) suggested that the mitochondrial pathway was primarily involved in the HY558-1-induced apoptosis in HeLa cells.

• 한국임상수의학회지
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• 제33권4호
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• pp.234-236
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• 2016

A 14 kg, 9-year-old, spayed female, Cocker Spaniel was presented to the Veterinary Medical Teaching Hospital of Seoul National University with a history of head tilt and circling. Otitis externa and media were diagnosed by computerized tomography, and total ear canal ablation was performed. In preanesthetic evaluation, systemic hypertension and second-degree atrioventricular block were observed, but there was no regurgitation through the heart valves. Systemic hypertension was managed with amlodipine (0.1 mg/kg, PO, BID) for the anesthesia. The dog was premedicated with cefazolin (22 mg/kg, IV) and midazolam (0.2 mg/kg, IV). Anesthesia was induced with alfaxalone (2 mg/kg, IV) and maintained with isoflurane and 100% oxygen following intubation. During surgery, vital signs (heart rate, respiratory rate, blood pressure, end tidal carbon dioxide partial pressure and body temperature) were maintained within normal ranges, but bradycardia was observed and corrected with glycopyrrolate (5 ug/kg IV, twice). During subcuticular suture, electrohemostasis was applied at the incision line, which was close to the trigeminal nerve. In no time at all, heart rate dramatically decreased from 110 to 60 beats per minute. No additional treatment was done because mean blood pressure was maintained above 70 mmHg. The heart rate recovered according to the decrease of end tidal isoflurane concentration and there were no complications associated with the anesthesia and surgery. Sudden bradycardia after electrical stimulation around the trigeminal nerve was considered as trigeminocardiac reflex (TCR). It is recommended to be careful of bradycardia from TCR when electrocautery is used in the craniofacial area during surgery.

• 치위생과학회지
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• 제11권1호
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• pp.31-35
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• 2011

본 연구는 치과 의료기관의 건강보험 청구실태를 파악하여 향후 효율적인 치과 건강보험 청구관리를 위한 기초자료를 제공하고자 2010년 4월 20일부터 6월 20일까지 서울, 인천 경기, 부산 울산 및 일부 지역에 소재한 치과 의료기관을 대상으로 설문조사를 실시한 결과 다음과 같은 결론을 얻었다. 1. 치과 건강보험 청구형태는 병원 내 인력을 활용하여 청구하는 단독청구가 45.4% 응답하였으며, 대행청구 54.6%이었다. 청구자의 직종은 치과위생사 78.2%, 일반사무원 7.2%, 간호조무사 6.5%, 치과의사 4.5%, 기타 3.5%로 조사되었다. 청구건수는 200건 이하가 23.9%, 201건-400건이 40.3%, 401건 이상이 35.8% 이었다. 2. 청구건수에 의한 분석에서 청구 건수가 적은 경우일수록 대행청구는 하는 의료기관이 76.3%로 많았고 청구건수가 많을수록 단독청구의 비율이 높아졌으며, 개원기간에 따른 분석에서는 단독청구를 하는 의료 기관은 개원기간이 5년 이하인 경우 82.7%, 개원기간이 16년 이상인 경우 31.3%로 개원기간이 짧을수록 단독청구를 하는 의료기관이 많았다(p<0.000). 3. 병원 인력 수에 따른 분석에서 4인 이하인 경우는 단독청구가 25.4%, 대행청구가 74.6%로 대행청구가 많았으나 9인 이상인 경우에는 단독청구가 71.1%, 대행청구가 32.6%이었다(p<0.000). 4. 치과위생사 수에 따라 청구방식의 차이를 분석한 결과 치과위생사가 없는 경우는 86.5%가 대행청구를하고 있었으며 치과위생사 수가 많아질수록 단독청구 방식을 선택하고 있었으며 통계적으로 유의한 차이가 있는 것으로 분석되었다(p<0.000). 5. 치과 의료기관에서 대행청구를 하는 이유를 분석한 결과 복잡한 청구절차가 28.0%로 가장 많았으며 다음으로 청구업무의 실력부족 22.6%, 청구업무인력의 인재부재가 20.8%이었다.

• 치위생과학회지
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• 제9권1호
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• pp.1-8
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• 2009

본 연구는 교정환자를 대상으로 의료서비스에 대한 만족도를 조사하여 교정환자의 다양한 요구와 치과의료기관의 경쟁력 강화를 위한 정보를 제공하고자 2008년 12월 3일부터 20일까지 부산지역 Y치과의원, 대구지역 B치과의원의 교정내원환자 226명을 대상으로 설문조사를 실시하였으며 SPSSWIN 14.0을 이용하여 분석한 결과 다음과 같은 결론을 얻었다. 1. 치과의원을 선택한 이유로는 '주위의 소개'가 50.7%로 가장 많았으며, 전체의 절반정도인 47.8%가 불만사항이 있을 때 '이야기 하는 편이다'라고 응답하였다. 교정치료 후 기대하는 것은 '가지런한 치아배열' 58.3%로 가장 많았으며, 교정치료 후 치아건강에 대한 기대로는 80.9%가 많이 혹은 아주 많이 나아질 것으로 기대하고 있었다. 교정치료 후 가장 하고 싶은 치료로는 미백이 70.6%로 가장 많았다. 2. 진료서비스 만족도에 유의한 영향을 미친 요인으로는 직원(치과위생사) 만족도가 가장 크고, 편의시설 만족도, 진료비 만족도, 치과의사 만족도, 친절성 만족도 순이었다. 3. 다른 사람에 대한 권유의사에 영향을 미친 요인으로는 진료비 만족도가 가장 크고, 치과의사 만족도, 직원(치과위생사) 만족도 순이었다. 교정환자가 원하는 치과의료서비스를 제공하기 위해서는 치과의료진의 지속적인 치과진료서비스 교육과 친절교육이 이루어져 교정환자의 요구도를 만족시켜야 할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1567-1574
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• 2014

The incidence and mortality rates of liver and nasopharyngeal cancer in Guangxi province of China have always been among the highest in the world, and cancer is one of the major diseases that pose a threat to the health of residents in Guangxi. However, no systematic study has been performed to evaluate the time trends in the structure of cancer-related deaths and cancer mortality. In this study, we reveal sex, age and geography differences of cancers mortality between three death surveys (1971 to 1973, 1990 to 1992, and 2004 to 2005). The results show that the standardized mortality rate of cancer in Guangxi residents has risen from 43.3/100,000 to 84.2/100,000, the share of cancer deaths in all-cause deaths has increased from 13.3% to 20.7%, and cancer has become the second most common cause of death. The five major cancers, liver cancer, lung cancer, gastric cancer, nasopharyngeal cancer and colorectal cancer, account for 60% of all the cancer deaths. Cancers with growing mortality rates over the past 30 years include lung cancer, colorectal cancer, liver cancer and female breast cancer, of which lung cancer is associated with the sharpest rise in mortality, with a more than 600% rise in both men and women. Cancer death in Guangxi residents occurs mainly in the elderly population above 45 years of age, especially in people over the age of 65. The areas with the highest mortality rates for liver cancer and nasopharyngeal cancer, which feature regional high incidences, include Chongzuo and Wuzhou. Therefore, for major cancers such as liver cancer, lung cancer, gastric cancer, nasopharyngeal cancer and female breast cancer in Guangxi, we can select high-risk age groups as the target population for cancer prevention and control efforts in high-prevalence areas in a bid to achieve the ultimate goal of lowering cancer mortality in Guangxi.

• 한국치위생학회지
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• 제9권4호
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• pp.685-698
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• 2009

Objectives : This study was to examine the recognition and understanding of the use, application of oral hygiene supplies among dental clinic patients in a bid to stress the necessity of education on the right awareness and use of oral hygiene supplies, to provide information on the development of educational programs and ultimately to help promote the oral health of people in general. Methods : The subjects in this study were 314 patients who visited dental clinics in North Jeolla Province in June 2009. Results : 1. Out of oral hygiene supplies, the largest number of the patients investigated(74.6%) were best cognizant of dental floss, and the greatest group(77.7%) had the right understanding of the use of toothpick. Currently, the oral hygiene supplies that were most widely in use were toothpicks(43.2%). 2. As to the relationship of awareness and understanding of the use, application of oral hygiene supplies to subjective oral health status, 50.0 percent of the patients who understood the use of toothpick found themselves to be in good health, and the gaps between them and the others were statistically significant. Among those who were aware of dental floss, the largest group(51.4%) considered themselves to be in good shape, and in the event of those who understood the use of dental floss, the greatest group(49.2%) deemed themselves to be in good health. Out of those who understood the use of mouse rinse, the largest group(53.7%) thought they were in good health(p<0.05). 3. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as parents to use oral hygiene supplies recommended by dental clinics(p<0.05). 4. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as patients to use oral hygiene supplies recommended by dental clinics(p<0.05). 5. The largest group of those who didn't put dental floss to use didn't use it for other reasons unspecified in the questionnaire, and the second greatest group of them didn't use it since it was so onerous to do that. There were statistically significant differences in the reason why they didn't use the oral hygiene supplies(p<0.05). 6. Awareness of dental floss and interdental brush, understanding of the use of the two and whether to use the two at present or not made statistically significant differences to whether they were likely to use the oral hygiene supplies in the future. And whether they were likely to use the oral hygiene supplies in the future was statistically significantly different according to awareness of mouse rinse and understanding of the use of it as well(p<0.05). Conclusions : Dental hygienists have to provide patients with various data of oral hygiene devices through oral health education and then only patients caring in dental clinics can choose the appropriate devices to claim for their own disease.

• 한국치위생학회지
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• 제10권5호
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• pp.819-829
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• 2010

Objectives : The purpose of this study was to examine factors affecting the effective demand of workers for dental treatment in a bid to provide some information on the improvement of oral health. Methods : The subjects in this study were 255 workers who were engaged in the pharmaceutical industry. A self-administered survey was conducted, and the collected data were analyzed by a spss(statistical package for the social science) win 12.0 program to find out their demographic characteristics, oral health awareness, oral health behavior and factors for the choice of a dental institution. Results : 1. Concerning the level of oral health awareness, 47.0 percent were at a low level(0 to three scores), and 35.2 percent were at an intermediate level(four to six scores). 2. As to oral health behavior involving a daily mean toothbrushing frequency, they brushed their teeth 2.65 times per day on the average. The most widely utilized oral hygiene supply was mouth rinse. Regarding oral health status, 53.7 percent weren't in good dental health, and just 23.0 percent got a dental checkup on a regular basis. 45.8 percent of those who didn't get a dental checkup cited time constraints as the reason. 26.0 percent received oral health education, and 91.4 percent were aware of the necessity of a corporate incremental dental care system. 3. Marital status, age and the reliability of dental institutions made differences to their choice of a dental institution. 4. As for the relationship between oral health awareness and the factors for the choice of a dental institution, oral health awareness had a statistically significant positive correlation to the reliability of dental institutions. Conclusions : The above-mentioned findings suggest that oral health education enables workers to have a correct knowledge on oral health, to change their own oral health behavior and to make the right choice of a dental institution in consideration of their oral characteristics. They should be urged to get a regular dental checkup not to develop chronic oral diseases so that they couldn't have to receive first-aid dental treatment. To promote the oral health of workers, oral health care personnels available should be utilized, and the incremental dental care system should be more vitalized to cut down on their effective demand for dental treatment.

• 한국치위생학회지
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• 제10권5호
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• pp.935-945
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• 2010

Objectives : The purpose of this study was to examine the knowledge of dental hygienists on infection control in dental office and their attitude toward that in a bid to provide some information on ways of enhancing the level of infection control in dental office. Methods : The subjects in this study were 220 dental hygienists who worked in dental hospitals and clinics in Busan and South Gyeongsang Province. A survey was conducted from May 17 to June 17, 2010, and the answer sheets from 183 respondents were analyzed with a SPSS WIN 12.0 program. Statistical data on frequency, percentage and mean were obtained, and t-test, Pearson correlation coefficient and one-way ANOVA were utilized. Results : They got a mean of $4.59{\pm}.68$ in six categories of infection control knowledge. They had the best knowledge on dental waste disposal, followed by hand washing, post-sterilization management, instrument disinfection and sterilization, surface management of dental equipment and wearing personal protective equipment. They got a mean of $3.99{\pm}.54$ in attitude, and they scored lowest in practice of surface management of dental equipment. Overall, they scored higher in every aspect of knowledge than in attitude(t=11.410, p=.0.000). There was the greatest gap between their knowledge and practice in surface management of dental equipment (t=13.885, p=0.000), and there was the smallest gap between their knowledge and practice in hand washing(t=5.460, p=0.000). And a positive correlation was found between knowledge and attitude, as better knowledge of infection control led to better attitude toward that(p<.001). Finally, concerning infection control knowledge and attitude by general characteristics, the presence or absence of infection control guidelines made differences to infection control knowledge, and infection control attitude was statistically significantly different according to infection control education experiences(t=6.501, p=.012) and the presence or absence of infection control guidelines(t=22.836, p=.000). Conclusions : In order to bolster infection control in dental office, the related system should be improved to legally require dental personnels to implement infection control. Every dental office must be furnished with infection control guidelines, and sustained education should be provided for dental hygienists to carry out infection control.

• 초등상담연구
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• 제10권1호
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• pp.95-108
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• 2011

이 연구는 초등학생의 직업포부를 형성하는 과정에 영향을 주는 요인들을 살펴 이에 대한 경험적 자료를 마련하는데 그 목적이 있다. 이러한 목적에 따라 초등학생의 개인적 특성 변인 중에서도 인지적 측면인 학업성취와 정의적 측면인 자아존중감, 환경적 측면인 부모의 학력을 변인으로 정하여 직업포부 수준과 어떤 관계가 있는지 연구문제를 설정하였다. 이 연구결과 성별에 따른 직업포부 수준은 여학생이 남학생보다 높게 나타났으며, 학년에 따라서는 통계적으로 유의미한 차이를 보이지 않았다. 그리고 초등학생의 학업성취는 직업포부 수준과정적인 상관관계를 보였으며, 자아존중감과 직업포부 수준의 관계는 통계적으로 유의하지 않았다. 학업성취와 자아존중감을 예측변인으로 했을 때 4학년의 경우에는 직업포부 수준의 9%, 6학년은 12%를 설명하였고, 부모의 학력에 따른 초등학생의 직업포부 수준은 통계적으로 유의미한 차이를 보이지 않았다. 특히 초등학생 4, 6학년이 직업의 특성뿐만 아니라 학업성취, 즉 자신의 능력을 함께 고려하여 직업포부를 결정한다는 것을 알 수 있었다.

• 한국건축시공학회지
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• 제10권1호
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• pp.163-173
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• 2010

최근 많은 FED프로젝트가 많이 진행되고 있습니다. 주한 미군기지 이전 계획이 발표됨에 따라 이에 참여할 건설업계는 발주기관 및 공구 수, 공구별 공사비, 입찰참가자격, 시공사 선정방법 등 구체적인 입찰사항에 대한 궁금증을 불러일으키고 있다. 사업 진행이 미군기지 이전시설 종합계획(MP) 수립 및 평택 주민 이주협의, 사업관리업체(PMC: Program Management Consortium) 선정 등 사업 초기부터 체계적으로 진행되지 않고 있어 계속 지연되고 있는 상황이다. 이는 물리적으로 군 시설이라는 특성에 시설공사에 까다로운 기준을 가진 주한미군이 공사를 공동으로 수행함에 따라 의사결정에 많은 시간이 소요되는 데다 주한 미군 공사를 수행하기 위한 지침 및 경험 부족으로 인하여 사업을 체계적으로 진행시키기 못하고 있다. 이에 따라 PMO(미군기지 이전사업 합동추진단:Program Management Office)는 건설사업관리용역업체(PMC:Program Management Consortium)로 CH2M HILL - 건원 컨소시엄을 선정하여 주한 미군기지 이전 사업을 종합적으로 관리하도록 하고 있다. 하지만 주한 미군 공사의 경우 FED(Far East District)와 국방부 조달본부에서 공동으로 관리하도록 되어 있어 효율적인 사업관리가 곤란한 실정이다. 또한 한미의 사업진행 문화의 이질성 및 세부실행지침 미비로 갈등이 발생하고 있으며, 이를 관리할 국내 전문가도 부족한 실정이다. 따라서 본 연구에서는 기존 행해졌던 FED 공사 사례를 바탕으로 하여 입찰부터 준공까지 각 단계별 정보의 흐름 및 수행 주체, 또한 각종 제한사항 및 규제 등을 분석하여 향후 미군 공사 수행에 있어서 기초 자료로 활용할 수 있도록 하고자 한다.