• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.978-984
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• 2004

In the course of screening for a novel inhibitor of CDC2, HY558-1 was isolated from a culture broth of Penicillium minioluteum F558. Moreover, it was found that HY558-1 had an effect on both the cell cycle regulation and apoptosis of human cervical adenocarcinoma HeLa cells. A flow cytometric analysis of HeLa cells revealed appreciable cell cycle arrest at the G1 and G2/M phases following treatment with HY558-1. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with HY558-1. To obtain further information on the cell cycle arrest and apoptotic induction induced by HY558-1, the expression of certain cell cycle and apoptosis-associated proteins was examined using a Western blot analysis. The results revealed that HY558-1 inhibited the phosphorylation of pRb and decreased the expression levels of CDK2, CDC2, and cyclin A in the cell cycle progression. It was also shown that the level of $p21^{WAF1/CIP1}$ was increased in HeLa cells treated with 0.52 mM of HY558-1. Accordingly, HY558-1 was found to inhibit the proliferation of HeLa cells through the induction of G1 phase arrest by inhibiting pRb phosphorylation via an upregulation of $p21^{WAF1/CIP1}$, and G2/M phase arrest by directly inhibiting CDC2 and cyclin A. Moreover, HeLa cells treated with 0.52 mM of HY558-1 exhibited apoptotic induction associated with the cleavage of Bid and release of cytochrome c from mitochondria into the cytosol. Subsequent investigation of the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) suggested that the mitochondrial pathway was primarily involved in the HY558-1-induced apoptosis in HeLa cells.

• Journal of Veterinary Clinics
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• v.33 no.4
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• pp.234-236
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• 2016

A 14 kg, 9-year-old, spayed female, Cocker Spaniel was presented to the Veterinary Medical Teaching Hospital of Seoul National University with a history of head tilt and circling. Otitis externa and media were diagnosed by computerized tomography, and total ear canal ablation was performed. In preanesthetic evaluation, systemic hypertension and second-degree atrioventricular block were observed, but there was no regurgitation through the heart valves. Systemic hypertension was managed with amlodipine (0.1 mg/kg, PO, BID) for the anesthesia. The dog was premedicated with cefazolin (22 mg/kg, IV) and midazolam (0.2 mg/kg, IV). Anesthesia was induced with alfaxalone (2 mg/kg, IV) and maintained with isoflurane and 100% oxygen following intubation. During surgery, vital signs (heart rate, respiratory rate, blood pressure, end tidal carbon dioxide partial pressure and body temperature) were maintained within normal ranges, but bradycardia was observed and corrected with glycopyrrolate (5 ug/kg IV, twice). During subcuticular suture, electrohemostasis was applied at the incision line, which was close to the trigeminal nerve. In no time at all, heart rate dramatically decreased from 110 to 60 beats per minute. No additional treatment was done because mean blood pressure was maintained above 70 mmHg. The heart rate recovered according to the decrease of end tidal isoflurane concentration and there were no complications associated with the anesthesia and surgery. Sudden bradycardia after electrical stimulation around the trigeminal nerve was considered as trigeminocardiac reflex (TCR). It is recommended to be careful of bradycardia from TCR when electrocautery is used in the craniofacial area during surgery.

• Journal of dental hygiene science
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• v.11 no.1
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• pp.31-35
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• 2011

The purpose of this study was to examine the state of the claim of dental clinics for payment from the national health insurance corporation in a bid to provide some information on the efficient management of payment claim by dental institutions. The findings of the study were as follows: As for the form of payment claim, 45.4 percent claimed payment by themselves, and 54.6 percent asked an agent to do that on behalf of them. Concerning the type of occupation of the applicants, dental hygienists demanded payment in the biggest number of the dental clinics(78.2%). The most common number of cases that the dental clinics demanded payment was between 201 and 400(40.3%). The dental clinics asked an agent to claim payment when the number of payment claim cases was smaller, and they claimed payment by themselves when the number of payment claim cases was larger. Regarding the reason why the dental institutions asked an agent for payment claim, the biggest group(28.0%) cited complicated claim procedure as the reason, and the second largest group(22.6%) answered that they weren't used to doing that. The third greatest group(20.8%) pointed out a shortage of personnels that would be responsible for that as the reason.

• Journal of dental hygiene science
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• v.9 no.1
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• pp.1-8
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• 2009

The purpose of this study was to examine the satisfaction level of orthodontic patients with medical service in a bid to provide information on the diverse needs of orthodontic patients and the improvement of the competitiveness of dental institutions. The subjects in this study were 226 orthodontic patients who visited Y and B dentist's offices respectively located in Busan and Daegu. A survey was conducted from December 3 to 20, 2008, and SPSSWIN 14.0 program was utilized to analyze the collected data. The findings of the study were as follows: 1. As to the reason why they chose the dentist's offices, the greatest group made that choice through the recommendation of people around them(50.7%). Approximately half them(47.8%) tended to talk about grievances when they had any, and the greatest group(58.3%) expected to have a regular set of teeth when they received orthodontic treatment (58.3%). And the largest group(80.9%) expected that treatment to improve their oral health a lot. The greatest group(70.6%) wanted to receive whitening treatment after completing orthodontic treatment. 2. As to factors significantly affecting their satisfaction level with treatment service, satisfaction level with the employees(dental hygienists) had the most significant impact, followed by satisfaction with amenities, medical fee, dentists and friendliness. 3. Their satisfaction level with medical fee exerted the most influence on intention of Recommendation offices they visited to others, followed by satisfaction with dentists and employees(dental hygienists).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1567-1574
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• 2014

The incidence and mortality rates of liver and nasopharyngeal cancer in Guangxi province of China have always been among the highest in the world, and cancer is one of the major diseases that pose a threat to the health of residents in Guangxi. However, no systematic study has been performed to evaluate the time trends in the structure of cancer-related deaths and cancer mortality. In this study, we reveal sex, age and geography differences of cancers mortality between three death surveys (1971 to 1973, 1990 to 1992, and 2004 to 2005). The results show that the standardized mortality rate of cancer in Guangxi residents has risen from 43.3/100,000 to 84.2/100,000, the share of cancer deaths in all-cause deaths has increased from 13.3% to 20.7%, and cancer has become the second most common cause of death. The five major cancers, liver cancer, lung cancer, gastric cancer, nasopharyngeal cancer and colorectal cancer, account for 60% of all the cancer deaths. Cancers with growing mortality rates over the past 30 years include lung cancer, colorectal cancer, liver cancer and female breast cancer, of which lung cancer is associated with the sharpest rise in mortality, with a more than 600% rise in both men and women. Cancer death in Guangxi residents occurs mainly in the elderly population above 45 years of age, especially in people over the age of 65. The areas with the highest mortality rates for liver cancer and nasopharyngeal cancer, which feature regional high incidences, include Chongzuo and Wuzhou. Therefore, for major cancers such as liver cancer, lung cancer, gastric cancer, nasopharyngeal cancer and female breast cancer in Guangxi, we can select high-risk age groups as the target population for cancer prevention and control efforts in high-prevalence areas in a bid to achieve the ultimate goal of lowering cancer mortality in Guangxi.

• Journal of Korean society of Dental Hygiene
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• v.9 no.4
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• pp.685-698
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• 2009

Objectives : This study was to examine the recognition and understanding of the use, application of oral hygiene supplies among dental clinic patients in a bid to stress the necessity of education on the right awareness and use of oral hygiene supplies, to provide information on the development of educational programs and ultimately to help promote the oral health of people in general. Methods : The subjects in this study were 314 patients who visited dental clinics in North Jeolla Province in June 2009. Results : 1. Out of oral hygiene supplies, the largest number of the patients investigated(74.6%) were best cognizant of dental floss, and the greatest group(77.7%) had the right understanding of the use of toothpick. Currently, the oral hygiene supplies that were most widely in use were toothpicks(43.2%). 2. As to the relationship of awareness and understanding of the use, application of oral hygiene supplies to subjective oral health status, 50.0 percent of the patients who understood the use of toothpick found themselves to be in good health, and the gaps between them and the others were statistically significant. Among those who were aware of dental floss, the largest group(51.4%) considered themselves to be in good shape, and in the event of those who understood the use of dental floss, the greatest group(49.2%) deemed themselves to be in good health. Out of those who understood the use of mouse rinse, the largest group(53.7%) thought they were in good health(p<0.05). 3. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as parents to use oral hygiene supplies recommended by dental clinics(p<0.05). 4. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as patients to use oral hygiene supplies recommended by dental clinics(p<0.05). 5. The largest group of those who didn't put dental floss to use didn't use it for other reasons unspecified in the questionnaire, and the second greatest group of them didn't use it since it was so onerous to do that. There were statistically significant differences in the reason why they didn't use the oral hygiene supplies(p<0.05). 6. Awareness of dental floss and interdental brush, understanding of the use of the two and whether to use the two at present or not made statistically significant differences to whether they were likely to use the oral hygiene supplies in the future. And whether they were likely to use the oral hygiene supplies in the future was statistically significantly different according to awareness of mouse rinse and understanding of the use of it as well(p<0.05). Conclusions : Dental hygienists have to provide patients with various data of oral hygiene devices through oral health education and then only patients caring in dental clinics can choose the appropriate devices to claim for their own disease.

• Journal of Korean society of Dental Hygiene
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• v.10 no.5
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• pp.819-829
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• 2010

Objectives : The purpose of this study was to examine factors affecting the effective demand of workers for dental treatment in a bid to provide some information on the improvement of oral health. Methods : The subjects in this study were 255 workers who were engaged in the pharmaceutical industry. A self-administered survey was conducted, and the collected data were analyzed by a spss(statistical package for the social science) win 12.0 program to find out their demographic characteristics, oral health awareness, oral health behavior and factors for the choice of a dental institution. Results : 1. Concerning the level of oral health awareness, 47.0 percent were at a low level(0 to three scores), and 35.2 percent were at an intermediate level(four to six scores). 2. As to oral health behavior involving a daily mean toothbrushing frequency, they brushed their teeth 2.65 times per day on the average. The most widely utilized oral hygiene supply was mouth rinse. Regarding oral health status, 53.7 percent weren't in good dental health, and just 23.0 percent got a dental checkup on a regular basis. 45.8 percent of those who didn't get a dental checkup cited time constraints as the reason. 26.0 percent received oral health education, and 91.4 percent were aware of the necessity of a corporate incremental dental care system. 3. Marital status, age and the reliability of dental institutions made differences to their choice of a dental institution. 4. As for the relationship between oral health awareness and the factors for the choice of a dental institution, oral health awareness had a statistically significant positive correlation to the reliability of dental institutions. Conclusions : The above-mentioned findings suggest that oral health education enables workers to have a correct knowledge on oral health, to change their own oral health behavior and to make the right choice of a dental institution in consideration of their oral characteristics. They should be urged to get a regular dental checkup not to develop chronic oral diseases so that they couldn't have to receive first-aid dental treatment. To promote the oral health of workers, oral health care personnels available should be utilized, and the incremental dental care system should be more vitalized to cut down on their effective demand for dental treatment.

• Journal of Korean society of Dental Hygiene
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• v.10 no.5
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• pp.935-945
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• 2010

Objectives : The purpose of this study was to examine the knowledge of dental hygienists on infection control in dental office and their attitude toward that in a bid to provide some information on ways of enhancing the level of infection control in dental office. Methods : The subjects in this study were 220 dental hygienists who worked in dental hospitals and clinics in Busan and South Gyeongsang Province. A survey was conducted from May 17 to June 17, 2010, and the answer sheets from 183 respondents were analyzed with a SPSS WIN 12.0 program. Statistical data on frequency, percentage and mean were obtained, and t-test, Pearson correlation coefficient and one-way ANOVA were utilized. Results : They got a mean of $4.59{\pm}.68$ in six categories of infection control knowledge. They had the best knowledge on dental waste disposal, followed by hand washing, post-sterilization management, instrument disinfection and sterilization, surface management of dental equipment and wearing personal protective equipment. They got a mean of $3.99{\pm}.54$ in attitude, and they scored lowest in practice of surface management of dental equipment. Overall, they scored higher in every aspect of knowledge than in attitude(t=11.410, p=.0.000). There was the greatest gap between their knowledge and practice in surface management of dental equipment (t=13.885, p=0.000), and there was the smallest gap between their knowledge and practice in hand washing(t=5.460, p=0.000). And a positive correlation was found between knowledge and attitude, as better knowledge of infection control led to better attitude toward that(p<.001). Finally, concerning infection control knowledge and attitude by general characteristics, the presence or absence of infection control guidelines made differences to infection control knowledge, and infection control attitude was statistically significantly different according to infection control education experiences(t=6.501, p=.012) and the presence or absence of infection control guidelines(t=22.836, p=.000). Conclusions : In order to bolster infection control in dental office, the related system should be improved to legally require dental personnels to implement infection control. Every dental office must be furnished with infection control guidelines, and sustained education should be provided for dental hygienists to carry out infection control.

• The Korean Journal of Elementary Counseling
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• v.10 no.1
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• pp.95-108
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• 2011

The purpose of this study was to examine variables affecting the occupational aspiration of elementary school students and the relationship of the variables in a bid to acquire empirical information on influential factors for the development of children's occupational aspiration. Out of the personal characteristics of school children, academic achievement, self-esteem and parental educational level that respectively belonged to the cognitive, affective and environmental domains were selected as the variables that might affect occupational aspiration. And the relationship of the variables to the level of occupational aspiration was selected as a research question of the study. The findings of the study were as follows: in relation to the level of occupational aspiration by gender, the girls were ahead of the boys. There were no statistically significant gaps in that aspect according to grade. And, the academic achievement of the elementary school students had a positive correlation to the level of their occupational aspiration, and there was no statistically significant relationship between self-esteem and the level of occupational aspiration. When academic achievement and self-esteem were selected as predictors, the two variables made a 8.5% prediction of the occupational aspiration of the fourth graders, and made a 12.3% prediction of the occupational aspiration of the sixth graders. There were no statistically significant differences in the level of occupational aspiration according to parental educational level. The fourth and sixth graders were found to have an occupational aspiration in consideration of not only the characteristics of occupations but their own academic achievement, namely their abilities.

• Journal of the Korea Institute of Building Construction
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• v.10 no.1
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• pp.163-173
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• 2010

A significant number of projects have been managed by US Army Corps of Engineers FED. Today, more information must be provided to the parties involved in the projects by phase than ever before. Specifically, USFK`s construction projects have been managed by FED(Far East District). Recently, however, large US private construction companies have been filling the role previously taken by FED. However, there is few construction managers to implement FED project in Korea. Accordingly, this study analyzes FED projects practices with focus on the construction management actors in order to suggest guideline. This study suggests a model FED construction process(Bid, Contract, Construction, As-Built Phase) by analyzing the existing FED project process using a IDEF0 methodology.