• 제목/요약/키워드: Berberis

검색결과 51건 처리시간 0.043초

초고압 저온처리에 의한 매자나무의 면역 활성 (Effect of Immune Activity on Berberis koreana Palibin by Ultra High Pressure Low Temperature Process)

  • 김영;한재건;하지혜;정향숙;권민철;안주희;김진철;최근표;정을권;이현용
    • 한국약용작물학회지
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    • 제16권6호
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    • pp.439-445
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    • 2008
  • This study was performed to investigate a methodology of improving immune activities of Berberis koreana Palibin by ultra high pressure on low temperature extraction process. Extraction yield was enhanced up to more than 35% by above process, compare to the control and extraction average. The cytotoxicity on human kidney cell (HEK293) was showed below 20.4%, 21.6% in adding 1.0 mg/ml of the highest concentration. Generally, the extracts by ultra high pressure extraction process showed less toxicity about 5% than the other processes. It could tell that toxic materials that existing in the plant could be reduced or broken by ultra high pressure process due to can be broken bond such the hydrogen bond, the electrostatic bond, the van der Waals bond, and the hydrophobic bond, can be broken by high pressure. The result could be employed to develop a new type of functional food from B. koreana Palibin by low temperature high pressure process.

Berberis amurensis complex의 형태 변이 분석 (Morphological Variation of Berberis amurensis Complex)

  • 현창우;김영동
    • 식물분류학회지
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    • 제38권2호
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    • pp.93-109
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    • 2008
  • 본 연구에서는 B. amurensis complex를 구성하는 세 분류군, 매발톱나무(B. amurensis Rupr. var. amurensis), 섬매발톱나무[B. amurensis var. quelpaertensis (Nakai) Nakai] 및 왕매발톱나무(B. amurensis var. latifolia Nakai)를 대상으로 형태 변이 분석을 수행하여 이들에 대한 기존의 분류학적 견해를 논의하였다. 분류군 간에 차이를 나타낼 것으로 조사된 22개 형질에 대한 변이 양상 조사 및 주성분분석을 수행한 결과 섬매발톱나무는 엽신의 길이와 폭, 엽정의 각도, 거치 간격, 절간 길이, 화서당 꽃의 수 등 여러 형질에서 나머지 두 분류군과 차이를 보였고, 왕매발톱나무는 엽정의 각도, 엽신의 폭과 길이 비에서 다른 두 분류군과 달랐다. 주성분분석 결과 엽신의 길이와 폭 및 엽정의 각도 등 잎의 형질이 분류군의 차이를 잘 반영하는 유용한 형질로 재확인되었으며, 절간의 길이와 화서를 구성하는 꽃의 수는 이들 종내 분류군들을 구분하는 새로운 식별형질로 밝혀졌다. 이들 세 분류군의 지리적 분포와 변이 양상 및 주성분분석 결과를 종합해 고려할 때 섬매발톱나무와 왕매발톱나무를 각각 변종으로 인정하는 것이 가장 타당할 것으로 판단하였다.

중부지방 낙엽조경수목의 열매색 특성 및 지속 기간에 관한 연구 (A study on the fruit color characteristics and persisting period of deciduous woody landscape plants in Middle area of Korea)

  • 서병기;심경구;정해준;심재성
    • 한국조경학회지
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    • 제23권3호
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    • pp.144-154
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    • 1995
  • The fruit color characteristics and persisting period of deciduous woody landscape plants were investigated through the field survey in Suwon region from January 1, 1992 to March 20, 1993. The summary of the study results was as follows; The total fruit color persisting period was about 320 days from May 1, 1992 when Prunus mume was beginning of fruit coloring, to March 20, 1993 when the fruits of Platanus occidentalis and platanus X acerifolia were persisting. And the plants of fruit persisting period over 60 days after leaf falling were Vibumum erosum, Ilex serrata, Ilex verticillata 'Christmas Cheer', Platanus X acerifolia, Platanus occidentalis, Berberis thunbergii 'Atropupurea', Ligustrum obtusifolium. According to the KBS standard color number, 52.6% of of the fruit color were red, 18.9% yellow, 11.6% black, green 9.5%, white 2.1%, violet 1.1%, and red is followed black 4.2%. Evodia daniellii, Ilex verticillata 'Christmas Cheer', Ilex serrata, Ginkgo biloba, Lindera obtusiloba, and Lindera erythrocarpa should be planted male and female species together for fruits. We got the new information on the fruit color characteritics and persisting period of Malus 'Hopa', Malus 'Almey', Malus 'Pioneer X', Acer rubrum, Malus prunifolia, Pyrus serotina, Berberis thunbergii 'Atropurpurea', Vibumum rhytidophyllum, Rosa spp. Vitis vinifera, Vaccinium angustifolium, Ilex verticillata 'Christmas Cheer', Magnolia stellata, Aronia arbutifolia, Sorbus alnifolia, Lonicera japonica var. aueroreticulta, and Ligustrum X vicaryi. And we need to introduce new cultivars of woody landscape plants including Malus spp, Berberis spp, Sorbus alnifolia yellow autumn leaf clone etc. for the better planting design. The fruit persisting period of woody landscape plants studied by fruit name was that sorosis 276 days, samara 155 days, legume 153 days, hip 133 days, pome was 124 days, drupe 92 days, berry 73 days, capsule 67 days, follicle 55 days and nut 52 days respectively.

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유산균 발효된 매자나무 추출물이 마우스 혈청중의 Melatonin 및 Serotonin의 함량에 미치는 영향 (The Effect of Extracts of Berberis koreana Bark by Lactobacillus Fermentation on the Concentration of Serotonin and Melatonin in the Serum of Treated ICR Mice)

  • ;김지선;서용창;최운용;안주희;마충제;윤창순;이현용
    • 한국약용작물학회지
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    • 제19권2호
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    • pp.117-125
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    • 2011
  • This study was to investigate the effect of fermentation extracts on the concentration of serotonin and melatonin in the serum of the ICR mice. The ICR mice were divided into water control group, lactobacillus fermentation solution including (Lactobacillus paracasei and Bifidobacterium longum B6) control group, positive control group (milk and doxylamine succinate), negative control group (caffein) and the groups treated with the extracts of Berberis koreana bark (WE: water extracts, FE-L.P: fermentation extracts of Lactobacillus paracasei, FE-B.L: fermentation extracts of Bifidobacterium longum B6). After ten-day feeding treatment, the mean concentration of serotonin for water control, WE, FE-L.P and FEB. L group was 134.72, 183.01, 232.09 and $223.78 ng/m{\ell}$, respectively. The mean concentration for FE-L.P and FE-B.L group were approximately 66% larger than that for water control group. The mean concentration of melatonin for water control, WE, FE-L.P and FE-B.L group was 76.92, 106.66, 157.56 and $141.81pg/m{\ell}$, respectively. The mean concentration of melatonin for FE-L.P and FE-B.L group were also larger than that for water control group. Our results indicated that the fermentation extracts of Berberis koreana bark have relatively greater potential to induce secretion of serotonin and melatonin. Therefore, the fermentation extracts have antidepressant effect.

초고압 및 초음파 추출공정을 이용한 매자나무 수피의 향장활성 증진 (Enhancement of Cosmeceutical Activities of Berberis koreana Bark by High Pressure and Ultrasonification Extraction Processes)

  • ;하지혜;최운용;서용창;김지선;김영옥;차선우;김진철;이현용
    • 한국약용작물학회지
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    • 제19권1호
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    • pp.54-65
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    • 2011
  • This study was performed to investigate the enhancement of cosmeceutical activities of Berberis koreana bark by different extraction processes. The extracts are WE (water extract at $100^{\circ}C$, control), USE (ultrasonification for 1 hours at $60^{\circ}C$ with water), HPE (high pressure for 5 minutes at $60^{\circ}C$ with water) and USE + HPE (ultrasonification process for 1 hours after high pressure for 5 minutes at $60^{\circ}C$ with water), respectively. The cytotoxicity of the extracts was in the range of 24.02~26.94% at 1.0 mg/ml concentration. The USE + HPE showed the lowest cytotoxicity. Compared to the WE, total phenolic and flavonoid contents in the USE + HPE increased to 121.5% and 154.2%. The USE + HPE showed the highest activity at 1.0 mg/ml concentration in 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, inhibition activity of xanthine oxidase and superoxide dismutase (SOD)-like test, respectively. Tyrosinase inhibition of WE, USE, HPE and USE + HPE at 1.0 mg/ml concentration was measured as 17.72, 19.62, 22.83 and 24.16%, respectively. Hyaluronidase inhibition activities of the USE + HPE were higher than 20.8%~29.5% of the WE. Our results suggested that the extracts from ultrasonification process after high pressure extraction has relatively high cosmeceutical activities, and that the bark of Berberis koreana could be considered as a candidate of new functional cosmetic agents.

화악산의 산림군락과 환경요인의 상관관계 분석 (Correlation Analysis between Forest Vegetation Type and Environment Factor in Mt. Hwaak)

  • 윤충원;김혜진;양희문;임종환;김영걸;신준환;이병천
    • 한국환경과학회지
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    • 제18권5호
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    • pp.579-588
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    • 2009
  • The purpose of this study was to explain relationship between community structure and their environment variables in Mt. Hwaak. Samples were collected by 101 plots using ZM phytosociological method and followed by cluster, importance value and canonical correspondence analysis. The forest vegetation classified into 8 community types such as Pinus densiflora community, Berberis amurensis community, Betula ermani community, Betula schmidtii community, Larix leptolepis community, Pinus koraiensis community, Cornus controversa community and Salix koreensis community. Altitude was considered as the highest factor correlated to the community types. Berberis amurensis community and Betula ermani community were located in upper slope area of high elevation, Comus controversa community and Salix koreensis community in valley area, and Pinus densiflora community in ridge area, respectively.

Diversity and Bioactive Potential of Culturable Fungal Endophytes of Medicinal Shrub Berberis aristata DC.: A First Report

  • Sharma, Supriya;Gupta, Suruchi;Dhar, Manoj K.;Kaul, Sanjana
    • Mycobiology
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    • 제46권4호
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    • pp.370-381
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    • 2018
  • Bioactive natural compounds, isolated from fungal endophytes, play a promising role in the search for novel drugs. They are an inspiring source for researchers due to their enormous structural diversity and complexity. During the present study fungal endophytes were isolated from a well-known medicinal shrub, Berberis aristata DC. and were explored for their antagonistic and antioxidant potential. B. aristata, an important medicinal shrub with remarkable pharmacological properties, is native to Northern Himalayan region. A total of 131 endophytic fungal isolates belonging to eighteen species and nine genera were obtained from three hundred and thirty surface sterilized segments of different tissues of B. aristata. The isolated fungi were classified on the basis of morphological and molecular analysis. Diversity and species richness was found to be higher in leaf tissues as compared to root and stem. Antibacterial activity demonstrated that the crude ethyl acetate extract of 80% isolates exhibited significant results against one or more bacterial pathogens. Ethyl acetate extract of Alternaria macrospora was found to have potential antibacterial activity. Significant antioxidant activity was also found in crude ethyl acetate extracts of Alternaria alternata and Aspergillus flavus. Similarly, antagonistic activity of the fungal endophytes revealed that all antagonists possessed inhibition potential against more than one fungal pathogen. This study is an important step towards tapping endophytic fungal diversity for bioactive metabolites which could be a step forward towards development of novel therapeutic agents.

저온고압 추출공정을 이용한 매자나무 수피의 항산화 및 항암활성 증진 (Enhancement of the Antioxidant and Anticancer Activities of Berberis koreana Bark by Using a Low Temperature and High-Pressure Extraction Process)

  • 김영;하지혜;정명훈;정을권;정애란;김진철;안주희;이현용
    • 한국식품과학회지
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    • 제41권3호
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    • pp.284-291
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    • 2009
  • 본 연구에서는 저온고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 저온고압 추출공정에 의한 매자나무 수피의 항산화활성과 항암활성의 증진을 확인하고자 연구를 수행 하였다. DPPH radical 소거 활성은 5분 저온고압 처리한 추출물이 93%로 가장 높은 활성을 나타내며 대조군인 ascorbic acid의 95%와 유사한 활성을 나타내었다. 저온고압 추출공정을 통한 매자나무 추출물이 일반 열수 추출물과 비교해 xanthine oxidase 저해활성이 높게 나타났으며 특히 5분 저온고압 처리한 추출물이 56.68%의 높은 저해 활성을 나타냈다. 따라서 저온고압 추출공정을 통해 매자나무 추출물의 항산화 활성의 증진이 이루어졌음을 알수 있다. 항암활성 측정 결과, 매자나무 수피 추출물의 높은 항암활성을 확인하였으며 5분 저온고압 처리한 추출물의 암세포 억제 활성이 가장 높게 나타남에 따라 저온고압 추출공정을 통해 항암활성의 증진이 가능함을 확인하였다. 세포독성 측정 결과 5분 저온고압 추출물이 정상 세포에 대한 독성이 가장 높은데 반해 15분 저온고압 처리한 추출물의 세포독성이 제일 낮은 것으로 미루어 저온고압 추출물의 세포독성이 저온고압 추출시간의 영향을 받는 것으로 사료된다. 따라서 매자나무 수피의 생체적용이 가능한 기능성 소재로서의 이용 가능성을 확인할 수 있으며 저온 고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 부여해 줄 수 있을 것으로 사료된다.

유산균 발효를 통한 매자나무 수피부의 항산화 활성 증진 (Enhancement of Antioxidant Activities of Bark of Berberis koreana Palibin by Lactic Acid Fermentation)

  • 하지혜;;서용창;최운용;김지선;김행훈;안주희;이현용
    • 한국약용작물학회지
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    • 제18권6호
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    • pp.421-428
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    • 2010
  • The bark of Berberis koreana Palibin was fermented by Bifidobacterium longum B6 and Lactobacillus paracasei at $37^{\circ}C$ for 72 hour, then extracted by water solvent at $100^{\circ}C$ for 180 min. Total pholyphenol and flavonoid contents were improved by fermentation process, compared to conventional water extraction. The barks of B. koreana Palibin extracts by B. longum B6 (FE-B.L.) and by L. paracasei (FE-L.P.) showed 17% and 16% cytotoxicity on human normal cell lines(HEK293) at 1.0 mg/$m{\ell}$ of the highest concentration, respectively, which was about 3~5% lower than 20% from normal extracts (NE). DPPH radical scavenging activity of the FE-B.L. and FE-L.P. were about 73% and 75.9% higher than 56.8% of NE. The highest inhibitory potency on xathine oxidase and superoxide dismutase (SOD)-like activities were also measured as 31.9% and 61.9% by adding FE-L.P. of 1.0 mg/$m{\ell}$. On tyrosinase inhibition test, the FE-L.P. showed highest activity as 75.9% at 1.0 mg/$m{\ell}$. Generally, FE-L.P. showed higher antioxidant activities as well as higher tyrosinase inhibition activity, possibly due to high contents of total pholyphenol and flavonoid. In general, fermentation of barks of B. koreana Palibin has relatively better biological activities than normal extracts. Specifically, the extracts fermented by L. paracasei showed higher activities than that from B. longum B6.