한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제18권6호
  • /
  • Pages.421-428
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  • 2010
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

유산균 발효를 통한 매자나무 수피부의 항산화 활성 증진

Enhancement of Antioxidant Activities of Bark of Berberis koreana Palibin by Lactic Acid Fermentation

  • 하지혜 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • ;
  • 서용창 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 최운용 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 김지선 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 김행훈 (농촌진흥청 농업과학기술원 농업유전자원센터) ;
  • 안주희 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 이현용 (강원대학교 바이오산업공학부 생물소재공학과)
  • Ha, Ji-Hye (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Jeong, Myoung-Hoon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Seo, Yong-Chang (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Kim, Ji-Seon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Kim, Haeng-Hoon (National Agrobiodiversity Center, NAAS, RDA) ;
  • Ahn, Ju-Hee (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology)
  • 투고 : 2010.06.28
  • 심사 : 2010.12.07
  • 발행 : 2010.12.30
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초록

The bark of Berberis koreana Palibin was fermented by Bifidobacterium longum B6 and Lactobacillus paracasei at $37^{\circ}C$ for 72 hour, then extracted by water solvent at $100^{\circ}C$ for 180 min. Total pholyphenol and flavonoid contents were improved by fermentation process, compared to conventional water extraction. The barks of B. koreana Palibin extracts by B. longum B6 (FE-B.L.) and by L. paracasei (FE-L.P.) showed 17% and 16% cytotoxicity on human normal cell lines(HEK293) at 1.0 mg/$m{\ell}$ of the highest concentration, respectively, which was about 3~5% lower than 20% from normal extracts (NE). DPPH radical scavenging activity of the FE-B.L. and FE-L.P. were about 73% and 75.9% higher than 56.8% of NE. The highest inhibitory potency on xathine oxidase and superoxide dismutase (SOD)-like activities were also measured as 31.9% and 61.9% by adding FE-L.P. of 1.0 mg/$m{\ell}$. On tyrosinase inhibition test, the FE-L.P. showed highest activity as 75.9% at 1.0 mg/$m{\ell}$. Generally, FE-L.P. showed higher antioxidant activities as well as higher tyrosinase inhibition activity, possibly due to high contents of total pholyphenol and flavonoid. In general, fermentation of barks of B. koreana Palibin has relatively better biological activities than normal extracts. Specifically, the extracts fermented by L. paracasei showed higher activities than that from B. longum B6.

키워드

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