• Title/Summary/Keyword: Bench-scale

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Characteristics of Glucose Oxidase Reaction of Onion Juice (양파 착즙액과 포도당 산화효소의 반응 특성)

  • Choi, Bong-Young;Lee, Eun-Mi;Kim, Young-Ran;Kim, Hyun-Jong;Chung, Bong-Woo
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.417-422
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    • 2003
  • The onions are considered to be a favorable functional source of beverage because they contain much sugar and various nutrients, and they are juicy vegetable. Recently, consumers have a new trend to take functional foods with health benefits. To meet this need, this study was the basic research to establish a manufacturing process of functional onion beverage by glucose oxidase. Glucose oxidase catalyzes reaction of glucose oxidation and makes generation of gluconic acid. Kinetics of the reaction was also investigated, and maximum glucose consumption rate $(V_{max})$ of $26.1{\times}10^{-2}\;g/L{\cdot}min$ and $K_m$ of 5.84 g/L were obtained. Optimum conditions were obtained when the glucose oxidase catalyzed reaction was carried out at temperature of $25^{\circ}C$, agitation rate of 450 rpm and aeration rate of 4 vvm in a 2.5 L jar fermentor. Finally, the enzyme reactor was 10-times scaled up and a similar glucose oxidation performance was achieved in the scaled-up reactor.

Drying Characteristic of High Moisture Coal using a Flash Dryer (기류건조기를 이용한 고수분 석탄의 건조 특성)

  • Kim, Sang Do;Lee, Si Hyun;Rhim, Young Joon;Choi, Ho Kyung;Lim, Jeong Hwan;Chun, Dong Hyuk;Yoo, Ji Ho
    • Korean Chemical Engineering Research
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    • v.50 no.1
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    • pp.106-111
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    • 2012
  • Drying characteristic of high moisture coal using a 5 kg/hr bench scale flash dryer was investigated. Moisture content and heating value of raw coal as received basis were 29.74 wt% and 4,270 kcal/kg, respectively. Gas inlet temperature and gas inlet flow rate were $400{\sim}600^{\circ}C$ and 10~20 m/sec, respectively. The raw coal was ground and classified to the particle size range of $100{\sim}2,000{\mu}m$. The moisture removal rate of raw coal was dramatically increased with increasing gas inlet temperature and decreasing gas inlet flow rate. The heating value of dried coal was increased to 5,100~5,900 kcal/kg. To examine the chemical change on the surface of high moisture coal during flash drying process, FT-IR spectral analysis was carried out. As a result, major changes in hydroxyl, carboxyl and carbonyl peak was confirmed.

Simultaneous Removal of Phenol and Hexavalent Chromium by Rhodococcus sp. CP01 (Rhodococcus sp. CP01에 의한 페놀과 6가 크롬이온의 동시 제거)

  • 최광현;오영숙;김병동;최성찬
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.279-284
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    • 2000
  • Simultaneous reduction of Cr(VI) and degradation of phenol was observed in batch and bench-scale continuous stirred tank reactors using Rhodococcus sp. CP01 isolated from leachate. The strain CP01, which was capable of utilizing phenol as a sole source of carbon and energy, completely reduced added hexavalent chromium (0.25 mM) to its trivalent form during 60 hr batch assay under optimal conditions (pH 7.0 and 1,000 mg/L of phenol concentration). The rates of Cr(VI) reduction and phenol degradation were estimated as 4.17 $\mu$M Cr(VI) and 38.4 mg phenol.$L^{-1}{\cdot}hr^{-1}$, respectively. The continuous culture experiment was conducted for 46 days using synthetic feed containing different levels of chromate (0.0625 to 0.25 mM) and phenol(1,000 to 4,000 mg/L). With a hydraulic retention time of 100 hr, Cr(VI) reduction efficiency was mostly 100% for influent Cr(VI) and phenol concentrations of 0.125 mM and 3,000 mg/L, respectively. During quasi-steady-state operation, specific rate of Cr(VI) reduction was calculated as 0.34 mg Cr(VI).g $protein^{-1}{\cdot}hr^{-1}$ which was comparable to reported values obtained by using glucose as growth substrate. The results suggest the potential application of biological treatment for detoxification of wastewater contaminated simultaneously with Cr(VI) and pheonol.

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Optimization of Ultrafiltration Process using $MIEX^{(R)}$+Coagulation Process ($MIEX^{(R)}$+응집공정을 이용한 한외여과 공정의 최적화 : 다양한 전처리 공정의 적용에 따른 막 오염 현상 규명)

  • Son, Hee-Jong;Hwang, Young-Do;Roh, Jae-Soon;Jung, Chul-Woo;Kang, Lim-Seok
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.7
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    • pp.753-761
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    • 2005
  • In this study, pretreatment of organic matters with $MIEX^{(R)}$ was evaluated using bench-scale experimental procedures on four organic matters to determine its effect on subsequent UF membrane filtration. For comparison, coagulation process was also used as a pretreatment of UF membrane filtration. Moreover, the membrane fouling potential was identified using different fractions and molecular weights of organic matters. From the removal property of MW organic matters by coagulation process for the sample water NOM and AOM, the removal efficiency of high MW organic matters were much higher than those of low MW organic matters. It was shown that the removal efficiency of high MW organic matter more than 10 kDa was lower than that of low MW organic matter for $MIEX^{(R)}$ process. For the change of permeate flux by the pretreatment process, $MIEX^{(R)}$+UF process showed high removal efficiency of organic matter as compared with coagulation-UF processes, but high reduction rate of permeate flux was presented through the reduction of removal efficiency of high MW organic matter. From sequential filtration test results in order to examine the effect of MW of organic matter on membrane fouling, it was found that the membrane foulant was occurred by high MW organic matter, and the DOC of organic matter less than 0.5 mg/L was working as the membrane foulant. In the case of sample water composed of low MW organic matter less than 10 kDa, since the low MW organic matter less than 10 kDa has high removal efficiency by $MIEX^{(R)}$, low reduction rate of permeate flux is obtained as compared with coagulation-UF processes. In summary, it is required to conduct the research on physical/chemical characteristic of original water before pretreatment process of membrane process is selected, and a pertinent pre-treatment process should be employed based on the physical/chemical characteristic of original water.

Novel Thin Film Composite Forward Osmosis Membranes of Highly Enhanced Water Flux with Interlayer Polysiloxane Between Polysulfone and Polyamide (폴리술폰과 폴리아미드 경계층에 형성된 폴리실록산을 이용한 정삼투 복합 박막의 유량 향상)

  • Jung, Boram;Kim, Nowon
    • Membrane Journal
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    • v.26 no.5
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    • pp.391-400
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    • 2016
  • In this work, novel thin film composite (TFC) forward osmosis (FO) membranes are developed via interfacial polymerization on the polysulfone (PS) substrate, using TEOS as the a sol-gel reagent to form hydrophilic interlayer polymer between PS and polyamide (PA). The PS substrate was cast on a very thin polyester nonwoven to reduce membrane resistance. With the incorporation of TEOS (tetraethoxy silane) polymer in the interface between PS and PA, the formed TFC FO membrane exhibits better hydrophilicity and improved water flux, and therefore superior membrane performance. By changing the polymerization sequence of PA interfacial polymerization and TEOS sol-gel condensation, the surface properties and performance of FO membranes are changed significantly. The permeability of FO membranes were estimated using the bench-scale FO test equipment. The distribution of the polysiloxane on composite membrane and morphology are also studied with FE-SEM and EDAX. The PS_PA_TEOS membrane showed highly enhanced water flux (79.2 LMH) but reverse salt flux (RSF) value (7.10 GMH) also increased. However, the flux of PS_TEOS_PA membrane increased moderately (54.1 LMH) without increasing RSF value (1.60 GMH) compare with PS_PA membrane.

Inactivation of Infectious Cryptosporidium parvum by Various Disinfectants (다양한 소독제에 의한 감염성 크립토스포리디움 불활성화율 평가)

  • Byun, Seung-Heon;Lee, Mok-Young;Cho, Eun-Ju;Yoon, Tae-Ho;Kim, Tae-Ho;Han, Sun-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.5
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    • pp.533-539
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    • 2007
  • Cell culture infectivity assay using HCT-8 cell was combined with most-probable-number technique to evaluate the inactivation of Cryptosporidium parvum by various disinfectants, including chlorine, ozone, and UV light. The assay was demonstrated to be as sensitive as animal infectivity assay, which has been considered the "gold standard" for assessing Cryptosporidium oocyst infectivity, and a valuable tool to evaluate inactivation of C. parvum by disinfectants. Bench-scale inactivation study showed that at the condition of $5^{\circ}C$ and pH 7.0, CT value of $1,250mg{\cdot}min/L$ by chlorine and $16mg{\cdot}min/L$ by ozone were required to achieve approximately 1.0 log inactivation of C. parvum, suggesting that even ozone could not be sufficient to inactivate C, parvum at low. temperature. Unlike chlorine and ozone, UV light is very effective to inactivate C. parvum, regardless of temperature. A UV light dose of 2 $mJ/cm^2$ provided at least 3 log inactivation of C. parvum.

Experimental Study on the Characteristics of Coal Gasification by 1 T/D BSU Coal-Slurry Entrained Gasifier (1 T/D급 습식 분류상 석탄가스화기에서의 석탄가스화 특성연구)

  • 박태준;김재호;손성근;이재구;홍재창;김용구;최영찬
    • Journal of Energy Engineering
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    • v.8 no.4
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    • pp.553-559
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    • 1999
  • This study has been implemented to investigate various characteristics of coals which are imported from abroad. KIER has developed 1 T/D bench-scale unit, entrained-flow coal-slurry gasification technology to investigate the followings: 1) to assess the appropriate foreign coals for gasification. 2) to establish the data base for gasification phenomena, 3) to minimize the technical risks prior to introduction of commercial scaled IGCC power plant, 4) to develop essential key technologies and to establish operational experiences for coal gasification. The foreign coals used in the gasification are Cyprus and Alaska coals from U. S. A. Cyprus coal(bituminous) and Alaska coal (lignite) were shown about 1.29$0^{\circ}C$. The concentrations of coal-slurry for Cyprus were maintained up to 58%, 62% and 65% in order to enable to feed satisfactorily it into the gasifier without any other problems at feeding systems. However, the Alaska coal was unable to maintain slurry concentration over the 60% due to its high viscosity. During the experiments, $O_2/coal$ ratios in both coals ere maintained from 0.6~1.2, but especially Alaska coal was required excessive oxygen feed due to its high ingerent moisture contents. During the experiments with two different coals, the concentrations of syngas $(H_2+CO)$ were shown as 40~62%, and the heating value of syngas were detected as 1,400~2,050 kcal/N㎥

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Experiment for Reduction Effectiveness of Pollutants with the Improvement of Infiltration Ability in Vegetation Filter Strip (식생여과대내 침투능력 향상에 따른 오염물질 저감효과에 대한 실험)

  • Lee, Young-A;Choi, I-Song;Oh, Jong-Min
    • Journal of Korea Water Resources Association
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    • v.40 no.2 s.175
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    • pp.183-190
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    • 2007
  • Soil layer in vegetation filter strip is one of the very important factor for reduction of non-point pollutants by physical, chemical and biological reactions of it through infiltration process. This study was carried out to prepare more effective vegetation filter strip through modification of soil layer for river water quality improvement. Therefore, the main aims of this study are to compare and evaluate normal (straighten type) and modified (step type) vegetation filter strip, which make artificially change the soil layer to improve infiltration ability, through bench scale experiments. In the results of this study, vegetation filter strip of step type is much more highly reduction effectiveness of pollutants in surface flow compared with normal vegetation filter strip. In case of below effluent, however, it appeared that the vegetation filter strip modified soil layer showed lower reduction effectiveness of pollutants than the general vegetation filter strip. This result was judged because effluent through the vegetation filter strip of step type passed bigger size of aggregate or sand than the vegetation filter strip of straighten type. If we compare it as a definition of pollutant load to estimate total amount of reduced pollutants by vegetation filter strip, reduced pollutants load by step type showed higher than those by straighten type because below effluent amount relied on total effluent amount was higher at step type (4%) than at straighten type (2%). In conclusion, the vegetation filter strip of step type to improve infiltration effect is much more reduction effectiveness of pollutants than vegetation filter strip of straighten type.

Enhancement of SNUF Active Trailing-edge Flap Blade Mechanism Design (SNUF뒷전 플랩 블레이드 메커니즘의 설계 개선)

  • Natarajan, Balakumaran;Eun, WonJong;Shin, SangJoon
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.23 no.7
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    • pp.645-653
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    • 2013
  • Seoul National University flap(SNUF) blade is a small-scale rotor blade incorporating a small trailing-edge flap control surface driven by piezoelectric actuators at higher harmonics for vibration attenuation. Initially, the blade was designed using two-dimensional cross-section analysis and geometrically exact one-dimensional beam analysis, and its material configuration was finalized. A flap-deflection angle of ${\pm}4^{\circ}$ was established as the criterion for enhanced vibration reduction based on an earlier simulation. The flap-linkage mechanism was designed and static bench tests were conducted for verifying the performance of the flap-actuation mechanism. Different versions of test beds were developed and tested with the designed flap and the selected APA 200M piezoelectric actuators. Through significant improvements, a maximum deflection of ${\pm}3.7^{\circ}$ was achieved. High-frequency experiments were conducted for evaluating the performance, and the transfer function of the test bed was determined experimentally. With the static tests almost complete, the rotor power required for testing the blade in a whirl tower (centrifugal environment) was calculated, and further preparations are underway.

Development of a Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect Hepatitis B Surface Antigen (HBsAg), Antibody to HBsAg, and Antibody to Hepatitis C

  • Ryu, Ji Hyeong;Kwon, Minsuk;Moon, Joung-Dae;Hwang, Min-Woong;Lee, Jeong-Min;Park, Ki-Hyun;Yun, So Jeong;Bae, Hyun Jin;Choi, Aeran;Lee, Hyeyoung;Jung, Bongsu;Jeong, Juhee;Han, Kyungja;Kim, Yonggoo;Oh, Eun-Jee
    • Annals of Laboratory Medicine
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    • v.38 no.6
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    • pp.578-584
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    • 2018
  • Background: Accurate, rapid, and cost-effective screening tests for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection may be useful in laboratories that cannot afford automated chemiluminescent immunoassays (CLIAs). We evaluated the diagnostic performance of a novel rapid automated fluorescent lateral flow immunoassay (LFIA). Methods: A fluorescent LFIA using a small bench-top fluorescence reader, Automated Fluorescent Immunoassay System (AFIAS; Boditech Med Inc., Chuncheon, Korea), was developed for qualitative detection of hepatitis B surface antigen (HBsAg), antibody to HBsAg (anti-HBs), and antibody to HCV (anti-HCV) within 20 minutes. We compared the diagnostic performance of AFIAS with that of automated CLIAs-Elecsys (Roche Diagnostics GmbH, Penzberg, Germany) and ARCHITECT (Abbott Laboratories, Abbott Park, IL, USA)-using 20 seroconversion panels and 3,500 clinical serum samples. Results: Evaluation with the seroconversion panels demonstrated that AFIAS had adequate sensitivity for HBsAg and anti-HCV detection. From the clinical samples, AFIAS sensitivity and specificity were 99.8% and 99.3% for the HBsAg test, 100.0% and 100.0% for the anti-HBs test, and 98.8% and 99.1% for the anti-HCV test, respectively. Its agreement rates with the Elecsys HBsAg, anti-HBs, and anti-HCV detection assays were 99.4%, 100.0%, and 99.0%, respectively. AFIAS detected all samples with HBsAg genotypes A-F and H and anti-HCV genotypes 1, 1a, 1b, 2a, 2b, 4, and 6. Cross-reactivity with other infections was not observed. Conclusions: The AFIAS HBsAg, anti-HBs, and anti-HCV tests demonstrated diagnostic performance equivalent to current automated CLIAs. AFIAS could be used for a large-scale HBV or HCV screening in low-resource laboratories or low-to middle-income areas.