• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1484-1489
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• 2014

A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at $10^{\circ}C$ (43% activity remained), with the optimal temperature at $20^{\circ}C$, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.1
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• pp.159-177
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• 2018

Participatory sensing is becoming popular and has shown its great potential in data acquisition for ambient assisted living. In this paper, we propose an incentive mechanism in participatory sensing for ambient assisted living, which benefits both the platform and the mobile devices that participated in the sensing task. Firstly, we analyze the profit of participant and platform, and a Stackelberg game model is formulated. The model takes privacy, reputation, power state and quality of data into consideration, and aims at maximizing the profit for both participant and publisher. The discussion of properties of the game show that there exists an unique Stackelberg equilibrium. Secondly, two algorithms are given: one describes how to reach the Stackelberg equilibrium and the other presents the procedures of employing the incentive strategy. Finally, we conduct simulations to evaluate the properties and effectiveness of the proposed mechanism. Simulation results show that the proposed incentive mechanism works well, and the participants and the publisher will be benefitted from it. With the mechanism, the total amount of sensory data can be maximized and the quality of the data can be guaranteed effectively.

• Geomechanics and Engineering
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• v.22 no.3
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• pp.255-264
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• 2020

With the ongoing development of deep mining of coal resources, some coal mine dynamic disasters have exhibited characteristics of both coal-gas outbursts and rockbursts. Therefore, research is required on the mechanism of rockburst-outburst coupling disaster. In this study, the failure characteristics of coal-rock combination structures were investigated using lab-scale physical simulation experiments. The energy criterion of the rockburst-outburst coupling disaster was obtained, and the mechanism of the disaster induced by the gas-solid coupling instability of the coal-rock combination structure was determined. The experimental results indicate that the damage of the coal-rock structure is significantly different from that of a coal body. The influence of the coal-rock structure should be considered in the study of rockburst-outburst coupling disaster. The deformation degree of the roof is controlled by the more significant main role of the gas pressure and the difference in the strength between the rock body and the coal body. The outburst holes and spall characteristics of the coal body after the failure of the coal-rock structure are strongly affected by the difference in strength between the roof and the coal body. The research results provide an in-depth understanding of the mechanism of rockburst-outburst coupling disasters in deep mining.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.277-287
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• 2018

Background: Temperature is an essential condition in red ginseng processing. The pharmacological activities of red ginseng under different steam temperatures are significantly different. Methods: In this study, an ultrahigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was developed to distinguish the red ginseng products that were steamed at high and low temperatures. Multivariate statistical analyses such as principal component analysis and supervised orthogonal partial least squared discrimination analysis were used to determine the influential components of the different samples. Results: The results showed that different steamed red ginseng samples can be identified, and the characteristic components were 20-gluco-ginsenoside Rf, ginsenoside Re, ginsenoside Rg1, and malonyl-ginsenoside Rb1 in red ginseng steamed at low temperature. Meanwhile, the characteristic components in red ginseng steamed at high temperature were 20R-ginsenoside Rs3 and ginsenoside Rs4. Polar ginsenosides were abundant in red ginseng steamed at low temperature, whereas higher levels of less polar ginsenosides were detected in red ginseng steamed at high temperature. Conclusion: This study makes the first time that differences between red ginseng steamed under different temperatures and their ginsenosides transformation have been observed systematically at the chemistry level. The results suggested that the identified chemical markers can be used to illustrate the transformation of ginsenosides in red ginseng processing.

• Journal of Microbiology
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• v.44 no.6
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• pp.685-688
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• 2006

The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.12-1,16-8. All of the strains expressed class 3 PorB protein. Among the five pulsed-field gel electrophoresis patterns observed, pattern III predominated.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.285.1-285.1
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• 2007

• 대한핵의학회:학술대회논문집
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• 2000.05a
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• pp.75.2-75.2
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• 2000

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.267.1-267.1
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• 2008

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.247.1-247.1
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• 2007

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.244.1-244.1
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• 2007