• Journal of Acupuncture Research
• /
• 제28권3호
• /
• pp.1-11
• /
• 2011

Objectives : This study is to compare effects on Intracutaneous Bee Venom pharmacopuncture and Intramuscular Bee Venom pharmacopuncture in Lumbar Disc Herniation. Methods : We investigated 34 cases of patients with lumbar disc herniation and devided into two groups. Group A were treated with intracutaneous bee venom pharmacupuncture and group B were treated with intramuscular bee venom pharmacopuncture. To evaluate the treatment effects of two groups, we used visual analog scale(VAS) and Aberdeen LBP scale. Results : In VAS and Aberdeen LBP scale, that of group A was further improved than that of group B, but there was no statistical significance. Conclusions : Intracutaneous bee venom pharmacupuncture and intramuscular bee venom pharmacopuncture were effective treatments for lumbar disc herniation, but there was no statistical significance.

• Journal of Acupuncture Research
• /
• 제19권3호
• /
• pp.41-50
• /
• 2002

Objective : To study anti-inflammatory, analgesic effect and molecular biological mechanism of honey bee venom for aqua-acupuncture, human mast cell line(HMC-1) and human glioma cell line(HS683) were treated with bee venom. Methods : Cell viability of bee venom was tested by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) asssay. To explore whether anti-inflammatory, analgesic effects of bee venom are associated with the control of gene expression, quantitative RT-PCR analysis of inflammation and pain related genes was performed. Results : The MTT assay demonstrated that cell viability was not decreased by treatment with 10-9 ug/ml bee venom in comparison with 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9, 10-10 and 10-11 ug/ml. sPLA2 and COX-l were down-regulated by treatment with 10-9 ug/ml bee venom in HS683 Cell line in comparison with control. COX-2 was up-regulated by treatment with 10-9 ug/ml bee venom in HS683 Cell line and HSP-2 was up-regulated by treatment with 10-9 ug/ml bee venom in HMC-1 Cell line in comparison with control. sPLA2, COX-1 and COX-2 showed no significant regulation in HMC-1 Cell line and cPLA2 also showed no significant regulation in both HMC-l and HS683 Cell line between control and bee venom treated group.

• Journal of Acupuncture Research
• /
• 제23권1호
• /
• pp.95-103
• /
• 2006

Objectives : This study was to evaluate the clinical effectiveness of Bee Venom acupuncture therapy for acute ankle sprain. Methods : From September 1st, 2005 to December 31st, 2005, the 60 patients who had visited Conmaul oriental medical hospital with acute ankle sprain were sample into 2 groups for this study; one group for the bee-venom acupuncture therapy combined with needle acupuncture therapy and the other group for needle acupuncture therapy only at least 3 times respectively. Both group had been treated with same additional infrared lamp, physical therapy and ankle supporter during the whole treatment period. Among 60 patients, 20 participants satisfied the whole inclusion criteria. To estimate the efficacy of treatments, Numerical Rating System(NRS) and Ankle-Hindfoot Scale(AHS) were applied before 1st and 4th treatment. Each score results were analysed and compared by Mann-Whitney test and Wilcoxon signed rank test with the level of 5% significance. Result : The NRS score in bee-venom acupuncture therapy group was increased significantly and the AHS score in bee-venom therapy group was decreased significantly compared to each of single acupuncture therapy group(P<0.01). The score change comparisons between the two groups had no significance before and after the treatment. Conclusion : Both bee-venom acupuncture therapy and single acupuncture therapy were effective to treat the acute ankle sprain. but there were no significant data to prove that bee-venom acupuncture therapy is more effective than single acupuncture therapy.

• Journal of Acupuncture Research
• /
• 제23권4호
• /
• pp.21-26
• /
• 2006

Objectives : This study was to evaluate the effectiveness of Bee Venom acupuncture therapy on chronic arthritis of ankle, Methods : We divided chronic arthritis of ankle patient into 2 group; one group combined bee venom acupuncture therapy and acupuncture therapy, another group was only acupuncture therapy. To estimate the efficacy of treatment that applied for two groups, we used visual analog scale(VAS). We compared the VAS score of two groups statistically. Results : 1. As a result of evaluation by using visual analog scale(VAS), treatment score at final was marked more higher than score before treatment on each groups. 2. treatment at final, acupuncture and bee venom acupuncture therapy group had significant result on visual analog scale(VAS) compared with acupuncture therapy group. Conclusion : Bee Venom acupuncture therapy can be used with acupuncture therapy for highly effective treatment for chronic arthritis of ankle.

• Journal of Acupuncture Research
• /
• 제26권4호
• /
• pp.115-123
• /
• 2009

Objectives : This study was to investigate the effectiveness of bee venom phamacopuncture complex therapy on the sequelae of peripheral facial palsy. Methods : We observed the effectiveness of bee venom pharmacopuncture complex therapy on three patients who have residual symptoms of Bell's palsy although early stage(0 to 3months) elapsed. H-B grade and Yanagiha's total score was used for evaluating the patient. Results : 1. Deviation of the bee venom therapy on the sequelae of peripheral facial palsy, One patient was improved from 19 to 39, another patient was improved from 25 to 40, the third patient was improved from 15 to 26 on Yanagiha's total score. 2. In the bee venom therapy on the sequelae of peripheral facial palsy, compared with baseline, at final, H-B grade and Yanagiha's total score was increased. Conclusions : Bee venom phamacopuncture can be available for relieving residual symptom of bell's palsy after the early stage.

• Journal of Acupuncture Research
• /
• 제23권4호
• /
• pp.15-20
• /
• 2006

Objectives : This study was to evaluate the effect of Bee venom-Acupuncture on knee joint with osteoarthritis. Methods : We investigated 30 cases of patients with osteoarthritis of knee joint. We flip a coin and divide into two groups. Bee venom-acupuncture was performed at one group, and the other group didn't do it. We evaluated the treatment effect of each group by using the WOMAC score. Results : 1. As a result of evaluation by using the WOMAC, the score after treatment was marked higher than that before treatment within each group. 2. After treatment, Bee venom-Acupuncture group showed significant difference on WOMAC score compared with acupuncture group. Conclusion : These results suggested that Bee venom-Acupuncture treatment should be more effective in the patient with osteoarthritis of knee joint.

• 대한약침학회지
• /
• 제9권1호
• /
• pp.139-154
• /
• 2006

Objective : This experiment was conducted to evaluate the effectiveness of Acupuncture, Bee Venom Acupuncture (BVA) and Bee Venom Herbal Acupuncture (BVHA) on post-stroke hemiplegic shoulder pain. Methods : 30 patients were randomly allocated into Acupuncture group, BVA group and BVHA group and was monitored weekly for 4 weeks; initial($T_0$), 1 week($T_1$), 2 weeks($T_2$), 3 weeks($T_3$) and 4 weeks($T_4$). Results : Visual analogue scale of shoulder pain showed significant decrease in BVA and BVHA groups compared to the Acupuncture group at T4 evaluation. Painless passive ROM of shoulder external rotation and Fugl-Meyer Motor Assessment of Upper Limb motor function showed significant increase in all groups. Modified Ashworth scale of the spasticity of upper limb showed no differences between the three groups. Conclusion : BVA & BVHA appears to be an effective in treating post-stroke hemiplegic shoulder pain. Further clinical studies must be done to obtain more concrete findings.

• Journal of Acupuncture Research
• /
• 제20권2호
• /
• pp.98-111
• /
• 2003

Objective : This study was designed to analyze the effects of bee venom and melittin on cell death in neuroblastoma cell line. Methods : MTT assay, morphologic method, DNA fragmentation, flow cytometry, immunocytochemistry analysis, RT-PCR and Western blot were performed. Results : The obtained results are summarized as follows: 1. The MTT assay demonstrated that neuroblastoma cell viability was significantly inhibitted dose-dependently by treatment with bee venom and melittin in comparison with control. 2. Cell culture demonstrated that control group proliferated highestly at he 5th day in comparison with the 4th day in bee venom and melittin group. And in bee venom and melitti group cell proliferation decreased 2.5 times than control group. 3. The morphologic study demonstrated that neuroblastoma cell showed apoptosis after treatment with bee venom and melittin for 6 hours using microscope. 4. The Flow cytometry demonstrated that apoptosis of neuroblastoma cell treated with bee venom and melittin was related with stop of cell cycle in stage of $G_0/G_1$. 5 .DNA fragmenation demonstrated that neuroblastoma cell treated with bee venom and melittin showed DNA ladder below 1 Kbp. 6. Immunocytochemistry assay demonstrated that Fos and MAPK which are related with cancer were down-regulated by treatment with bee venom and melittin. 7. RT-PCR analysis demonstrated that Fos and MAPK mRNA were transcripted. Fos was down-regulated form treatment with $5{\mu}g/ml$ bee venom and MAPK was down-regulated form $1{\mu}g/ml$ bee venom. 8. Western blot demonstrated that Fos was down-regulated from $1{\mu}g/ml$ bee venom whereas MAPK was expressed by $1{\mu}g/ml$ bee venom but down-regulated by $10{\mu}g/ml$ bee venom. Conclusions : We found that some cancer related genes ware down-regulated by treatment with bee venom and melittin. Further study is needed for investigating the anti-cancer effect of bee venom and melittin.

• Journal of Acupuncture Research
• /
• 제22권2호
• /
• pp.19-32
• /
• 2005

Objectives : to evaluate the analgesic effect of bee venom acupuncture on Choksamni (ST36) in the collagen-induced arthritis rats and investigate the role played by serotonergic receptor subtypes (5-HT1a, 5-HT2a) in the antinociceptive effect of bee venom acupuncture in a thermal hyperalgesia test Methods : Experiments were performed on 5 week-aged 60 male Sprague-Dawley rats according to National Institute of Health guidelines and the ethical guidelines of the International Association for the Study of Pain (IASP). Arthritis was induced with arthrogenic collagen emulsion (Bovine type II collagen ${\mu}g$ with incomplete Freund's adjuvant $100\;{\mu}g$). The onset of arthritis was considered to be present when erythema and swelling were detected in at least one joint. The thermal hyperalgesia was evaluated weekly with tail flick test in the rats of severity grade 3 without any injury at tail and foot (including inflammation, ulceration, snap). In the fourth week after first immunization, the analgesic effect of bee venom acupuncture (Choksamni, ST36) was measured with consecutive tail flick latency after intraperitoneal injection of spiroxatrine (1mg/kg) and spiperone (1mg/kg). Results : Chronic inflammatory pain was induced as time elapsed after the immunization of arthrogenic collagen and the maximum value was reached from third to fifth week. Chronic inflammatory pain induced by CIA was inhibited by bee venom acupuncture on the left ST36. The analgesic effect of bee venom acupuncture was inhibited by intraperitoneal injection of 5-HT1a antagonist spiroxatrine and 5-HT2a antagonist spiperone. Conclusions : Therefore, a conclusion. that the analgesic effect of bee venom acupuncture in the chronic inflammatory pain is partially mediated by 5-HT1a and 5-HT2a receptors can be made.

• 대한약침학회지
• /
• 제7권2호
• /
• pp.91-96
• /
• 2004

Objective : Alopecia areata is a common disease and the difficulty of its medical management is well known. This study was designed to investigate the effect of bee venom and Carthami Flos herbal acupuncture. Method : The patient was managed by bee venom, carthami-flos herbal acupuncture and General acupunture. Herbal acupuncture was injected subcutaneously into the lesion. We checked involvement in plaque, according to evaluation of the effect on alopecia areata. Results : After 5 month of treatment, the lesion had been replaced with new terminal hair. Evaluation of the effect on alopecia areata The score changed from 0 to 3 point. Conclusion : These result suggest that bee venom and Carthami Flos herbal acupuncture has good effect on alopecia areata. But further studies are required to concretely prove the effectiveness of bee venom and Carthmi Flos herbal acupuncture for treating alopecia areata.