• 한국산림과학회지
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• 제101권1호
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• pp.77-82
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• 2012

표고를 재배하기 위하여 톱밥배지를 $65^{\circ}C$로 저온살균하는 과정은 살균시설비용이 적게들고 배지성분도 보전되는 장점이 있으나, 오염 확률이 높아서 효과는 일정하지가 않았다. 본 연구는 참나무 톱밥배지의 저온살균 후 성공률이 높은 효과적인 배양조건을 알고자 하였다. 참나무톱밥배지를 $65^{\circ}C$, $100^{\circ}C$, $121^{\circ}C$로 살균한 후, 표고톱밥종균을 접종하고 $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$에서 배양하거나, 표고종균의 접종간격을 1 cm, 2 cm, 4 cm(표면적 비율은 4%, 11%, 25%)로 하여 표고균사의 생장과 배지의 오염정도를 비교하였다. 저온살균된 배지에서 표고균사생장은 배양온도 $25^{\circ}C$ 이하에서는 $100^{\circ}C$나 $121^{\circ}C$ 살균된 배지에서 처럼 양호하였으나, $30^{\circ}C$ 배양에서는 표고균사생장이 크게 위축되었다. 그리고 저온살균배지에서 표고종균 접종밀도를 1 cm 간격으로 하였을 때에는 배지가 표고균사로 완전점유되었으나, 2~4cm 간격 접종밀도에서는 14~33%의 배지가 오염되었다. 따라서 저온살균배지는 $25^{\circ}C$ 이하에서 25% 이상의 충분한 종균을 접종하여 배양하는 것이 성공적이라고 결론짓는다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.147-150
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• 2003

Water toxicity monitoring based on the continuous cultivation of Photobacterium phorphoreum is presented. Normally, after 10 days of operation, a dark variant, which emits no light, appears and dominates the population, resulting in a rapid decrease in bioluminescence. Therefore, to overcome this problem, a fluidized-bed reactor is used in which alginate-immobilized cells are grown and leaking cells are continuously released into the effluent Experimental results revealed that the dominance of dark variants was suppressed inside the immobilized beads, thereby mitigating the rapid loss of bioluminescence. Plus, a high dilution rate (1.2 h$\^$-1/) prevented the occurrence of other microbial contamination in the reactor The concentration and bioluminescence of the released cells were sufficient to measure the water toxicity for more than 4 weeks.

• 한국산림과학회지
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• 제96권4호
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• pp.401-407
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• 2007

본 연구는 표고버섯 재배의 정책 자료를 제시하고자 표고버섯 주산단지의 재배농가 총 88호를 대상으로 표고버섯 재배에 대한 경제성을 분석하였다. 표고버섯 재배농가의 평균 생산비는 151,699원/100본으로 분석되었고, 이 가운데 중간재비가 56.0%, 노동비용이 41.8%를 차지하였다. 노동집약적인 표고버섯 산업의 특성상 노동투입량에 따라 평균 생산비는 최고인 충북지역과 최소인 경기지역 사이에는 37.2%의 큰 격차가 있었다. 평균 조수입은 178,627원/100본으로 평균 경영비 116,613원/100본을 제외하면 평균 소득은 62,014원/100본, 평균 소득률은 34.7%로 분석되었다. 생산량, 품등, 경영비 특히 노동비용의 차이 등에 의해 소득률이 최고인 전북지역과 최소인 경기지역 사이에는 15.8%포인트 격차를 보였다. 이러한 분석결과 국내 표고버섯 산업의 경쟁력 향상을 위해서는 생산비에서 가장 높은 비중을 차지하고 있는 조성비 및 노동투입비용 절감이 최우선 과제라 할 수 있다.

• 한국지역지리학회지
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• 제17권2호
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• pp.141-149
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• 2011

사자평 고산습지는 유역분지 및 기반암의 특성과 화전의 영향을 받아 형성되었다. 사자평 유역분지는 출구가 좁고, 기반양은 많은 암설을 생산하여 사면 아래로 공급한다. 그리고 화전을 위하여 삼림을 제거함으로써 표토의 세립물질이 사전천의 하상에 퇴적되어, 시전천의 유로 주변에 고산습지가 형성되었다 사자평의 습원에는 도로를 따라 우곡이 형성되었는데, 우곡은 습원으로 공급되는 사력물질과 통류의 공급을 차단함으로써 습원을 건조화 시킨다. 이를 방지하기 위해서는 배수로의 수위를 기반암 풍화층의 높이까지 높여 습원으로 통류가흐를 수 있도록 하여야 하며, 배수로의 모양을 자연스러운 불규칙한 형태로 조정하여야 하고 시전천의 하상에 인위적인 방해물을 적치하여 시전천의 하상이 낮아지는 것을 방지하여야 한다.

• 한국산림과학회지
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• 제84권3호
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• pp.361-368
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• 1995

표고버섯 원목(原木) 재배(栽培)에 있어서 골목화 기간을 단축하여 해균(害菌)의 침입을 막고 생산량 증대를 위한 방법으로 생리활성화물질(生理活性化物質)이 표고버섯 골목화에 미치는 영향을 실험분석하였다. 생리활성화물질은 파(Allium fistulosm), 보리(Hordeum vulagare var. hexastichon), 표고버섯(Lentinus edodes), 당근(Daucus carota var. sativa), 유자(Citrus junos)를 사용하였는데 그 중 파(Allium fistulosm)의 추출액이 제일 유효하였으며, 생리활성화물질(生理活性化物質)에 효과가 높은 수종(樹種)은 굴참나무(Quercus variabilis)로 나타났다.

• 농업과학연구
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• 제39권1호
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• pp.35-41
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• 2012

This research was conducted to investigate the influence of organic bed soil substrates on growth and yield of organically grown ginseng transplantation in a shaded plastic houses. The pH and EC of the substrates used for this study were 5.93-6.78 and 0.03-0.15 dS/m, respectively. The concentrations of NH4-N and $NO_3$-N were 14.01-68.63 mg/L, 5.60-58.83 mg/L respectively. and the average quantum in the shaded plastic houses was 11-15% of natural light. The maximum temperature in the shaded plastic houses is higher ($3-7^{\circ}C$) than that of outside open field from the last part of April to early in August. Emergence date of ginseng was on March 21 in the mongolian type shaded plastic house, and was on March 29 in normal type shaded plastic house. Both roots and shoot growth of ginseng were excellent in the bed soils with PPV-2, compared with other compounds used. We concluded that the PPV-2 could be promising a good bed soil substrate for organic ginseng cultivation in shaded plastic house.

• 한국농공학회논문집
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• 제56권6호
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• pp.149-158
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• 2014

The effects of spot cooling on growing ever-bearing strawberry in hydroponic cultivation during summer by spot cooling system was estimated in plastic greenhouse located in Pyeongchang. The temperature of cooling water was controlled by heat pump and maintained at the range of $15{\sim}20^{\circ}C$. Cooling pipes were installed in root zone and very close to crown. Spot cooling effect was estimated by applying system in three cases which were cooling root zone, crown plus root zone, and crown only. White low density polyethylene pipe in nominal diameter of 16 mm was installed on crown spot, and Stainless steel flexible pipe in nominal diameter of 15A was installed in root zone. Crown and root zone cooling water circulation was continuously performed at flowrates of 300 ~ 600 L/hr all day long. Strawberry yields by test beds were surveyed from Aug. 1 to Sep. 30. The accumulated yield growth rate compared with a control bed of crown cooling bed was 25 % and that of crown plus root zone cooling bed was 25 % and that of root zone cooling bed was 20 %. The temperatures of root spot in root zone cooling was maintained at $18{\sim}23.0^{\circ}C$ and that of crown spot in crown cooling was maintained at $19{\sim}24^{\circ}C$. Also, the temperatures of root spot in crown plus root zone cooling bed was maintained at $17.0{\sim}22.0^{\circ}C$ and that of crown spot was maintained at $19{\sim}25^{\circ}C$.

• 한국작물학회지
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• 제44권2호
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• pp.97-101
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• 1999

No-till direct seeding cultivation of rice has major advantages such as saving of labor and cost by eliminating tillage, preparation of seed bed and trans-planting procedure compared to the conventional transplanting cultivation. A field experiment was conducted to evaluate the effects of straw treatment and nitrogen levels on the rice growth in no-till direct-seeding cultivation. Rice straw, vetch straw, and the mixture of both of the straws were mulched on the surface of soil before seeding while 4 levels of nitrogen fertilizer, 0, 7, 9, and 11 kkg/10a respectively, were applied at 3 split times, 3-weeks after sowing, 5-weeks after sowing and the panicle initiation stage. Mulching of vetch straw significantly reduced seedling establishment of rice which may be attributed to low oxidation-reduction potential of soil by vetch mulching treatment. Vetch straw increased the concentration of soil ammonium leading to an extension of the greenish leaf to panicle initiation stage. Agronomic nitrogen use efficiency (AD $E_{N}$) in heavy-mixed straw mulching plots was lower than other treatments. Grain yield and AU $E_{N}$ in the vetch treatment were less affected by fertilized N levels. Conclusively, it is suggested that heavy straw mulching was not efficient for rice seedling establishment and nitrogen usage.e.

• 한국균학회지
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• 제46권4호
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• pp.495-503
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• 2018

2016년 4월부터 6월까지 표고 원목재배 임가 4곳과 표고 톱밥재배 임가 1곳의 재배사 내 실내 공기에서 진균을 포집 및 분리 동정하였다. 총 6개의 국내 미기록 진균(Cenangium acuum, Periconia macrospinosa, Neopestalotiopsissurinamensis, Metarhiziummarquandii, Trichoderma petersenii, Trichoderma paratroviride)을 분리하였으며 이들 균류에 대하여 형태학적 특성 및 internal transcribed spacer rDNA region, large subunitregion, ${\beta}-tubulin$ 또는 translation elongation factor $1{\alpha}$유전자 염기서열에 기반하여 계통학적 분석 결과를 기술하였다.

• The Plant Pathology Journal
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• 제38권5호
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• pp.472-481
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• 2022

Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.