• Journal of Microbiology and Biotechnology
• /
• v.26 no.5
• /
• pp.909-917
• /
• 2016

The shiitake mushroom industry has suffered from Camptomyia (gall midges) pest, which feeds on the mycelium of shiitake mushroom during its cultivation. It has been postulated that fungal damage of shiitake bed-logs is associated with infestation by the insect pest, but this is not well understood. To understand the fungal damage associated with Camptomyia pest, various Trichoderma species were isolated, identified, and characterized. In addition to two previously known Trichoderma species, T. citrinoviride and T. deliquescens, two other Trichoderma species, T. harzianum and T. atroviride, were newly identified from the pestinfested bed-log samples obtained at three mushroom farms in Cheonan, Korea. Among these four species, T. harzianum was the most evident. The results of a chromogenic media-based assay for extracellular enzymes showed that these four species have the ability to produce amylase, carboxyl-methyl cellulase, avicelase, pectinase, and ß-glucosidase, thus indicating that they can degrade wood components. A dual culture assay on PDA indicated that T. harzianum, T. atroviride, and T. citrinoviride were antagonistic against the mycelial growth of a shiitake strain (Lentinula edodes). Inoculation tests on shiitake bed-logs revealed that all four species were able to damage the wood of bed-logs. Our results provide evidence that the four green mold species are the causal agents involved in fungal damage of shiitake bed-logs infested by Camptomyia pest.

• Journal of Forest and Environmental Science
• /
• v.14 no.1
• /
• pp.138-144
• /
• 1998

To provide further information for reutilization of the bed-logs decayed by oak mushroom(Lentinus edodes), the properties of wood-based board prepared with mixed materials of normal wood and bed-logs were examined. Wood fibers in bed-logs showed different morphology from normal wood fibers, that is, shorter length and rough surface. Thickness swelling increased with increasing mixing ratio of bed-log. On the other hand, modulus of rupture(MOR) in bending strength decreased. In products, the visual surface of board prepared from higher mixing rate of bed-log chips was smooth. Consquently, it is considered that the optimum mixing rate of bed-log to normal wood is the range of 25-50% and the bed-log can be used for raw material of board instead of normal wood.

• Korean journal of applied entomology
• /
• v.35 no.1
• /
• pp.45-51
• /
• 1996

As the pest of the Letinus edoeds, five insect species and six animal species are investigated injuring the fresh mushroom. Of these, insect and animals, Achorutes armatus, Scaphidium amurense and Philomycus confusa caused severe damage to mushroom fruitbody, and a greate deal of damage was done to mushroom lamella by mites (Rhizoglyphis sp., and Histiostoma sp.). As the pest of Lentinus edodes bed logs, two insect species from immature bed logs, eight insects and one isopod species from matured bed logs were investigated. Of those, Moechotypa diphysis was found to be the most injurious species from both immature and matured bed logs. As the pest of Pleurotus ostreatus, six insects and the two mite species were investigated from sporophore. Of those, Lycoriella sp. and Mycophilla sp. are considered to be very serious. while two insects and five mite species were investigated from the mushroom bed. Of those, one insect (Lycoriella sp.) and two mites (Histiostoma feroniarum and Throglyphus bankslogior) found to be abundant and caused serious damage.

• The Korean Journal of Mycology
• /
• v.35 no.1
• /
• pp.33-36
• /
• 2007

An attempt was made to investigate the status of harmful microorganisms occurring on different kinds of oak bed-logs during shiitake cultivation. As a result, totally 14 species of harmful microorganisms, including Trametes versicolor, were confirmed. Twelve kinds of harmful microorganisms were observed on Quercus acutissima, 9 kinds on Q. mongolica and 10 kinds on Q. aliena. Diatrype stigma, Hypoxylon truncatum, Hypoxylon sp. and Trichoderma sp. occupied 75.1% of the total harmful fungi occurred on Q. acutissima. H. truncatum and Trichoderma sp. occupied 71.2% of the total harmful fungi occurred on Q. mongolica. On Q. aliena, the occurrence of H. truncatum, Trichoderma sp. and Hypoxylon howeianum was 80.3%. D. stigma and Hypoxylon sp. were observed exclusively on Q. acutissima bed-logs, and the outbreak ratios were 51.6% and 13.1%, respectively. H. truncatum was observed on 46.6% of Q. aliena bed-logs and Trichoderma sp. was observed on 30.3% of Q. mongolica bed-logs.

• The Korean Journal of Mycology
• /
• v.21 no.3
• /
• pp.217-223
• /
• 1993

The investigation was aimed to reduce the period of bed log through the protection of harmful fungi and to know the effects of physiological activating substances on the yield increase of Lentinus edodes. Mycelial growth was evaluated both the bed logs and physiological activating substances, respectively. I percent extract of Allium cepa and malt as a physiological activating substance was highly effective in mycelial growth of Lentinus edodes. Better effects among the bed logs by the application fo physiological activating substances observed in both Quercus serrata and Carpinus laxiflora. This results suggested that supplement of physiological activating substances to the bed log would be beneficial for the production of Lentinus edodes.

• Korean Journal of Plant Resources
• /
• v.18 no.3
• /
• pp.512-517
• /
• 2005

To establish the economical cultivation method of Gastrodia elata Blume by controlling the shading rate and the mixing ratio of recycling spawn bed logs, a serial experiment was carried out. When G. elata was grown in the field with no shading, the yield of spawn tuber was higher than that of tuber of G. elata. The yield of G. elata was high in the rainproof green house covered with one layer of $95\%$ shading net, in the rainproof green house covered with two layers of $95\%$ shading net, and in the field with no shading, in that order. In the case of mixing ratio of recycling spawn bed logs, the yield was high in the fresh logs, in the mixing ratio of 2:1, and the ratio of 1:1, in that order. The income of the rainproof green house covered with one layer of $ 95\%$ shading net which brought the best yield was $81\%$ in the mixing ratio of 2:1 over the fresh logs.

• Journal of Mushroom
• /
• v.14 no.3
• /
• pp.119-126
• /
• 2016

Five empirical farmhouses were selected to reduce the high temperature damage in oak mushroom cultivation using bed-logs. The cultivation facilities were categorized as follows: those having two blackout curtains or one blackout curtain and outdoor oak mushroom cultivation. The inequality of the indoor condition, oak mushroom hyphae rampant ratio, and fruit body production in each test condition was evaluated. $3^{\circ}C$ was lower in indoor temperature of cultivation facility having two blackout curtains than one blackout curtain. Specifically, the indoor air humidity average of cultivation facilities having one or two blackout curtains was 10% lower than that of outdoor oak mushroom cultivation. This condition is not ideal for oak mushroom cultivation as continuous indoor humidity control is essential for producing good fruit bodies. The Inoculated bed-log surface and oak mushroom hyphae rampant ratio of bed-logs cultivated with two blackout curtains was superior to other tested conditions. The mushroom production ratio observed in facilities with two blackout curtains was 117-204% higher than those cultivated in facilities with only one blackout curtain. Furthermore, the mushroom production ratio increased in based on these findings, we recommended five cultivation facility models to reduce high temperature damage in oak mushroom cultivation using bed-logs.

• Korean Journal of Plant Resources
• /
• v.9 no.1
• /
• pp.1-9
• /
• 1996

The effects of multipore spawn inoculation of Lentinus edodes on the mycelial growth stage and on the quantitative and qualitative yields of fruit-bodies for 5 years were investigated at natural environmental conditions. The spawn inoculation quantity did not affect the mycelial rooting at a range of 3.0kg-6.0kg per $m^3$ of bed-logs while the increase of spawn quantity by multipore inoculation increased the mycelial growth and the inner erosion of bed-logs, resulting in the increased yields of total fruit-bodies of L. edodes per $m^3$ of bed-logs for 5 years but resulting in the shortened major production period of fruit-bodies to the the first three years. Major production of fruit-bodies occurred during May-June and August-September, which was not affected by the spawn inoculation quantity. No significant differences in dry weight of a fruit-body were observed as increasing the spawn quantity by multipore inoculation; however the heaviest dry weight of a fruit-body was 1.63g at the spawn inoculation of 4.0kg per $m^3$/TEX> of bed-logs.

• Economic and Environmental Geology
• /
• v.27 no.3
• /
• pp.289-294
• /
• 1994

This paper describes an effective utilization method of well logging data and boring cores for understanding the geology of Keoje-do area. Six holes were drilled in 1988-1989, and all rock cores were recovered. Nevertheless, seven kinds of logs were carried out in each borehole. The geologic situations of the drilling sites are accurately evaluated from a comparison between core descriptions and well logging data. Porosities and clay contents of sedimentary rocks can be calculated using the data of density and gamma-ray logs. Fractured zones are easily detected from the change in inside diameter of borehole by caliper log. Sonic, density and gamma-ray logging data clearly indicate alternated and dyke zones; the former can be detceted from an acoustic wave attenuation and a decline of compaction by sonic and density logs, the latter can be detected from the amount of potassium contents of bed rock and dyke by gamma-ray log.

• The Korean Journal of Mycology
• /
• v.31 no.2
• /
• pp.53-58
• /
• 2003

Studies were conducted to investigate the changes of teleomorph of Diatrype stigma on bed-log of shiitake (Lentinula edodes) and appearance of secondary harmful fungi from December 1999 to August 2001. The color of stroma was changed from red brown to dark brown after 12 months. The size of ostioles became smaller and the number of ostioles decreased as time passed. Most of the ostioles disappeared after 20 months from toleomorph formation. Perithecia and ascospores were gradually disappeared, and perithecia containing ascospores were observed at 10% of bed-logs tested after 20 months. Concerning the secondary harmful fungi infecting the bedlog of shiitake after teleomorph of D. stigma, Hypoxylon truncatum (Anamorph : Nodulisporium sp.), Schizophyllum commune and Trichoderma spp. were observed on stroma of D. stigma. Penicillium sp. occurred on spore-horn of Libertella sp. (Anamorph of D. stigma). Besides, Hypoxylon howeianum and some species of Myxomycetes were observed. Also, Libertella sp. appeared again at some bed-logs.