• Food Science and Preservation
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• v.25 no.1
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• pp.145-154
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• 2018

The purpose of this study was to investigate the ability of natural antioxidants as stabilizers for meat by-products to prevent lipid oxidation. The white internal organs were evaluated using different treatments: no antioxidant (control), ascorbic acid (T1), Artemisiacapillaris Thunb. (T2), Opuntia (T3), Schisandra chinensis (T4), and Saururuschinensis (Lour.) Baill (T5). Antioxidant activities were analyzed by measuring DPPH contents, superoxide anion radical levels, nitrate scavenging activities, and total polyphenol contents. T1 and T2 showed higher antioxidant activities and total polyphenol contents (p<0.05). Additionally, changes in physicochemical properties (pH, color, volatile basic nitrogen [VBN], and thiobarbituric acid reactive substances [TBARS]) and microbiological aspects in white internal organs processed with antioxidants were investigated. As storage time increased, the CIE $a^*$ and $b^*$ values of the white internal organs processed with natural antioxidants were decreased (p<0.05), and CIE $L^*$ values were low, particularly for the T3 sample relative to that in the control. Moreover, the pH, VBN, and TBARS values of samples T2-T5 were increased after 7 days of storage, but showed low values compared with those of the control (p<0.05). Moreover, compared with the control group, the treatments showed antimicrobial effects. Our results indicated that these natural antioxidants could be used as lipid oxidation stabilizers of meat by-products during storage and that Artemisiacapillaris Thunb. and Opuntia may have applications as natural antioxidants in the meat by-product industry.

• Korean Journal of Plant Resources
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• v.13 no.3
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• pp.227-242
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• 2000

Site characteristics, vegetation structure and dynamics of Kalopanax septemlobus forests in Mt. Heungjeong, Mt. Balwang and Mt. Gariwang were studied to provide the information need for national resources conservation and restoration of K. septemlobus forest in Gangwon-do of Korea. The K. septemlobus forests were distributed at 780m to 1,300m in altitude, steep slope, ridges-slope areas with facing northeast to northwest in good nutrition area. The annual diameter growth of those trees ranged 1.60mm to 2.41mm. Forty-three plots(20$\times$20m) were set up at three natural population of Kalopanax septemlobus forest and TWINSPAN method was used for vegetation structure analysis. The result of TWINSPAN ordination show that geographical features played a role in determining community types in the study areas. Community type of the study areas classified into three groups by TWINSPAN; K. septemlobus-Quercux mongolica- Acer mono community(Mt. Balwang), K. septemlobus-Q. mongolica- Tilia amurensis community(Mt. Heungjeong), K. septemlobus-Betula costata-Q. mongolica community(Mt. Gariwang). In most of the K. septemlobus forests, major woody species appeared predominantly were Q. mongolica, T. amurensis, Acer pseudo-sieboldianum, Lindera obtusiloba, A. mono, Magnolia sieboldii, Ulmus davidiana var. japonica. And also, Those species were surveyed in all the areas. Species diversity(H') of investigated areas ranged 0.3124~0.13992 and this value relatively higher than that of other forests with similar site. The range of similarity indices between surveyed areas was 64.1~64.54%. The distribution pattern of Morista's index show that Kalopanax septemlobus distributed randomly in tree and concentrated a few sample plots in subtree layer and shurb layer. K. septemlobus occupied 37.1~58.8% of tree layer story, 8.4~17.6% of subtree layer, 0~0.38% of shurb layer each surveyed area. The association analysis showed that Kalopanax septemlobus positively associated with Betula ermani, Deutzia glabrata, Carpinus cordata, Fraxinus mandshurica and negatively associated with Symplocos chinensis var. pilosa, Styrax obassia, Acer mono, Euonymus macroptera, Ulmus davidiana var. japonica. According to the diameter distribution of Kalopanax septemlobus community, the community of Mt. Gariwang will gradually decline and replaced by Quercus community. The communities of Mt. Heungjeong and Mt. Balwang has its seedlings and saplings, so it may be sustained.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.312-323
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• 1999

External morphology, principal component analysis, cluster analysis, and vegetation were investigated to understand the taxonomic relationships and native environmental characters of Megaleranthis saniculifolia populations in four different habitats. Morphological characters such as calyx length and width, calyx index, presence or absence of serrate in calyx lobe, length of peduncle, branch of peduncle and fruit characters were useful for the identification of four different habitats. But, characters of plant height, bract and seed in four habitats were similar. The results obtained based on the principal component(PC) analysis of treated 96 OTU were divided into two groups by PC 1, 2, 3, and the sums of contributions for the total variance were 66.79%(PC1 31.3%, PC2 20.7%, PC3 15.8%, respectively), and only Mt. Taeki population was distinctly different from populations of other three habitats. In cluster analysis based on average linkage cluster analysis and Ward's method, there were similarities in the composition of clustered taxa, and each populations were not identified. Importance value by relative coverage and frequency appeared in M. saniculifolia(50.81%), Aruncus dioicus(12.64%), Corydalus turtschanovii(11.62%), Veratrum oxysepalum(11.45%), Anemone koraiensis(8.96%), Meehania urticifolia(8.76%), Filipendula palmata(7.06%), Aconitum pseudo-laeve(5.66%), Pseudostellaria palibiniana(5.45%) and Smilacina japonica(5.25%), respectively. These species were considered to be highly similar with M. saniculifolia. The highest importance value in all investigated sites was M. saniculifolia, but specific composition of high level different from each habitat. Average diversity of species was 1.40, and the highest in Mt. Kwangdeok(1.31), lowest in Mt. Jumbong(1.17). Average soil pH was 5.25 and similar in each habitat. Although the lowest content of K ion, but the highest the EC, water capacity, organic compound, Ca content were found in soil of Mt. Sobaek. Soils in Mt. Kwangdeok had the lowest content of EC, organic compound, Ca. Soils of Mt. Jumbong showed the highest of Mg content, but the lowest of water capacity, $P_2O_5$, and K contents.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.145-152
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• 2015

The present study aimed to investigate comparative anti-inflammatory effects of Litsea japonica fruit and leaf extract considering the balance of safety and efficacy. Dose response studies were performed to determine the inhibitory effects of 70% EtOH extract of leaf (L70%) on the pro-inflammatory enzymes expression, COX-2/PGE2 and NO/iNOS, and pro-inflammatory cytokines production, IL-$1{\beta}$, IL-6, and TNF-$\alpha$ in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We also examined comparative effects of 30 and 70% EtOH extract of fruits (F30% and F70%) at low concentration ($10{\mu}g/ml$ ) in the same conditions. L70% at 50 and $100{\mu}g/ml$ showed inhibitory effects on almost all the inflammatory mediators we examined except for COX-2 regulation, but there were no effects at $10{\mu}g/ml$. Since $100{\mu}g/ml$ of L70% have 18.2% cytotoxicity, we compared the effects of fruit extract, F30% and F70% at $10{\mu}g/ml$ on the regulation of NO/iNOS, PGE2, IL-$1{\beta}$, IL-6, and TNF-$\alpha$ and obtained that fruit extacts are more efficacious and safe than leaf. This study suggests that the 30% EtOH fraction of L. japonica fruit could be a good candidate for development as a functional food supplement in the prevention of inflammatory disorders.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.116-122
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• 2009

Heteropappus arenarius Kitam., an autumn-flowering biennial belonging to wild chrysanthemums, is found to be native in southeastern coastal area and Jeju island of Korea. It could play a good role for ground cover plants on a large-scale landscape area, especially, barren soil or sloping hillside. This study was initiated to screen optimum germination temperature influenced by local strain and harvesting stage of H. arenarius. The following was the response of seed germination between local strain and temperature. The average of final germination percentage (FG) was the highest in 'Guryongpo' (89.7%) among four local strains, followed by 'Gujwa' (87.3%), 'Gampo' (87.3%), and 'HKNU-I' (71.5%). The average of $T_{50}$ was shorter in 'Gujwa' (3.6 d) and 'Guryongpo' (4.0 d) than the others. The average of FG and $T_{50}$ was the highest as 76.2% and shortest as 3.6 d in $20^{\circ}C$, respectively, followed by $30^{\circ}C$, $25^{\circ}C$, and $15^{\circ}C$. In case of 'Gujwa', however, FG and T50 was higher in $20^{\circ}C$ and shorter in $15^{\circ}C$ than others. In the relationship between harvesting stage and temperature, the average of FG was greatly higher in Stage III (90.7%) and Stage IV (88.6%) than the others including Stage II (35.7%) and Stage I (26.0%). The average of $T_{50}$ was shorter in Stage IV (3.7 d) and Stage III (4.3 d) than the others, which showed less than 50% of FG. Nevertheless, the available range of seed harvesting stage was from Stage I to Stage IV because H. arenarius seeds could germinate at all stages. In conclusion, it was recommended that the optimum temperature and harvesting stage was $20^{\circ}C$ and Stage $III{\sim}IV$, respectively, for seed germination of H. arenarius.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.396-418
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• 2018

This study was carried out to investigate the flora and the vegetation of Samusan mountain on Jecheon-si, located in the middle part of the Korean peninsula. The vascular plants which were collected in 9 times from June 2014 to October 2015 were identified as 502 taxa in total, including 102 families, 314 genera, 445 species, 6 subspecies, 49 varieties, 1 form and 1 hybrid. The largest families were as follows; Asteraceae (70 taxa), Poaceae (52 taxa), Rosaceae (30 taxa), Fabaceae (28 taxa), and Cyperaceae (20 taxa). Of them, Korean endemic plants numbered 10 taxa, and vascular plants listed in the red data according to the International Union for Conservation of Nature (IUCN) numbered 14 taxa. The floristic regional indicator plants found in this area were 61 taxa in total. Among them, 5 taxa revealed the floristic grade V, 11 for floristic grade IV, 14 for floristic grade III, 10 for floristic grade II, and 21 for floristic grade I. The alien plants were identified as 43 taxa and the percentage of naturalized index (NI) was 8.6%, and urbanization index (UI) was 13.4%, respectively. Samples of the forest vegetation on the Samusan Mt. were mainly classified as Pinus densiflora, Quercus variabilis, Q. acutissima, Q. mongolica, Zelkova serrata and Robinia pseudoacacia forest. The vertical structures of the forest were stable and the DBH-Class analyses showed that the dominant tree species would be maintained. In the surveyed areas, high plant diversity was shown, and a number of endemic, rare, calcicole plants and phytogeographically important plants were found. Nonetheless, numerous and diverse biological resources native have been consistently disturbed or damaged by human activities without some form of protection. Therefore, it is needed to set up strategies for conservation forest vegetation in this study area.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.363-371
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• 2018

Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at $5^{\circ}C$), warm stratification (0, 4, 8, or 12 weeks at $23^{\circ}C$, followed by 8 weeks at $5^{\circ}C$, and then incubation at $23^{\circ}C$), and $GA_3$ treatments (0, 10, 100, or 1000 mg/L). The treated seeds were incubated on aseptic media at room chamber ($23^{\circ}C$, a 16h photoperiod of fluorescent lamps with $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures ($23^{\circ}C$). $GA_3$ application overcame seed dormancy and promoted germination. Following $GA_3$ treatment (0, 10, 100, or 1000 mg/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and $GA_3$ treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.378-383
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• 2018

This study was carried out to investigate the optimum cultivation distance for high yielding cultivation of the new variety of Gomchwi in highland and lowland. The test material was used 'Sammany' variety and the area was cultivated with a highland of 750 m above sea level and a lowland of 20 m above sea level. The planting distance was 5 treatments of $20{\times}20cm$, $20{\times}30cm$, $30{\times}30cm$, $35{\times}40cm$ and $40{\times}40cm$, and the plant was cultivated at 35% shading net in the open field. The annual growth of 'Sammany' variety was higher in the highland than in the lowland, but the number of leaves was higher in the lowland than that in the highland. The leaf number per plant was the highest in the $40{\times}40cm$ plot and the more the planting distance was, the more tendency was. However, the leaf weight per plant was higher in the highland than in the lowland. The number of leaves per 10a were higher in the lowland than that of highland and the tendency was more as the planting distance was narrower. Yields were slightly higher in the highland than that of lowland and the highest treatment plot was 2,983 kg/10a in $20{\times}20cm$. In the second year, the growth was high in the highland, and the highland was high in the leaf number and leaf weight and the same tendency in the yields. Among the planting distances, Yields in both areas were the highest in the $20{\times}20cm$ treatment, with 3,369 kg/10a in lowland and 7,257 kg/10a in highland. The growth of the third year was slightly better in the highland than that of the lowland but the difference was not significant. However, the number of leaves and leaf weight per plant were higher in the lowland than that of highland. And, in terms of leaf number and yield per 10a, it was also higher in the lowland than that in the highland. The narrower the planting distance was increased yields, and the highest yield was 6,051 kg/10a in $20{\times}20cm$. The lowland was high in yield until the third year, but the highland was the highest in the second year and decreased slightly in the third year. The narrower the planting distance in the both areas, the higher the yield, and the leaf size tended to be small.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.303-311
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• 2018

In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, $PGE_2$, IL-6, and $IL-1{\beta}$) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the $CH_2Cl_2$ and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and $CH_2Cl_2$ fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_2$). The $CH_2Cl_2$ fraction also inhibited the production of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6, and $IL-1{\beta}$). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.

• Geophysics and Geophysical Exploration
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• v.8 no.1
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• pp.7-17
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• 2005

The determination of seismic velocities in refractors for near-surface seismic refraction investigations is an ill-posed problem. Small variations in the computed time parameters can result in quite large lateral variations in the derived velocities, which are often artefacts of the inversion algorithms. Such artefacts are usually not recognized or corrected with forward modelling. Therefore, if detailed refractor models are sought with model based inversion, then detailed starting models are required. The usual source of artefacts in seismic velocities is irregular refractors. Under most circumstances, the variable migration of the generalized reciprocal method (GRM) is able to accommodate irregular interfaces and generate detailed starting models of the refractor. However, where the very-near-surface environment of the Earth is also irregular, the efficacy of the GRM is reduced, and weathering corrections can be necessary. Standard methods for correcting for surface irregularities are usually not practical where the very-near-surface irregularities are of limited lateral extent. In such circumstances, the GRM smoothing statics method (SSM) is a simple and robust approach, which can facilitate more-accurate estimates of refractor velocities. The GRM SSM generates a smoothing 'statics' correction by subtracting an average of the time-depths computed with a range of XY values from the time-depths computed with a zero XY value (where the XY value is the separation between the receivers used to compute the time-depth). The time-depths to the deeper target refractors do not vary greatly with varying XY values, and therefore an average is much the same as the optimum value. However, the time-depths for the very-near-surface irregularities migrate laterally with increasing XY values and they are substantially reduced with the averaging process. As a result, the time-depth profile averaged over a range of XY values is effectively corrected for the near-surface irregularities. In addition, the time-depths computed with a Bero XY value are the sum of both the near-surface effects and the time-depths to the target refractor. Therefore, their subtraction generates an approximate 'statics' correction, which in turn, is subtracted from the traveltimes The GRM SSM is essentially a smoothing procedure, rather than a deterministic weathering correction approach, and it is most effective with near-surface irregularities of quite limited lateral extent. Model and case studies demonstrate that the GRM SSM substantially improves the reliability in determining detailed seismic velocities in irregular refractors.