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http://dx.doi.org/10.7732/kjpr.2018.31.4.303

Antioxidant and Anti-inflammatory Effects of Ficus erecta var. sieboldii Leaf Extract in Murine Macrophage RAW 264.7 Cells  

Jung, Yong-Hwan (Biodiversity Research Institute, Jeju Technopark (JTP))
Ham, Young-Min (Biodiversity Research Institute, Jeju Technopark (JTP))
Yoon, Seon-A (Biodiversity Research Institute, Jeju Technopark (JTP))
Oh, Dae-Ju (Biodiversity Research Institute, Jeju Technopark (JTP))
Kim, Chang-Suk (Biodiversity Research Institute, Jeju Technopark (JTP))
Yoon, Weon-Jong (Biodiversity Research Institute, Jeju Technopark (JTP))
Publication Information
Korean Journal of Plant Resources / v.31, no.4, 2018 , pp. 303-311 More about this Journal
Abstract
In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, $PGE_2$, IL-6, and $IL-1{\beta}$) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the $CH_2Cl_2$ and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and $CH_2Cl_2$ fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_2$). The $CH_2Cl_2$ fraction also inhibited the production of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6, and $IL-1{\beta}$). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.
Keywords
Antioxidant; Anti-inflammatory; Ficus erecta var. sieboldii; Oxidant stresses; Pro-inflammatory factors;
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