• Journal of Microbiology and Biotechnology
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• v.19 no.2
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• pp.128-135
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• 2009

Acinetohacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene was cloned and expressed in Escherichia coli BL21(trxB) as an inclusion body, which was subsequently solubilized by urea, and then purified using Ni-affinity chromatography. After being purified, the lipase was refolded by incubation at $4^{\circ}C$ in the presence of a 1:10 molar ratio of lipase:chaperone. The maximal activity of the refolded lipase was observed at a temperature of $35^{\circ}C$ and pH 8.3 when p-NP caprate(C10) was used as a substrate; however, 28% of the activity observed at $35^{\circ}C$ was still remaining at $0^{\circ}C$. The stability of the purified enzyme at low temperatures indicates that it is a cold-adapted enzyme. The refolded lipase was activated by $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$, whereas $Zn^{2+}\;and\;Cu^{2+}$ inhibited it. Additionally, 0.1% Tween 20 increased the lipase activity by 33%, but SDS and Triton X-100 inhibited the lipase activity by 40% and 70%, respectively.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.5
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• pp.61-72
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• 2019

The purpose of this study is to propose the clue of planning guideline for the elderly welfare center supporting the healthy life of the elderly through deriving the planning element of the elderly welfare center based on the Salutogenic Model(SM). The research method was proceeded with literature review. The meaning of Sense of Coherence(SOC) and Generalised Resistance Resources(GRR) which constitute Salutogenic Model is established. The correlation between SOC and GRR is analyzed. The conclusions of this study are as follows. First, EBD, BD, HD, UD, and BFD were studied as planning theories that could enhance the SOC of space. Second, based on the five planning theories, 43 planning elements needed for the elderly welfare center plan were derived. Third, as a result of classifying the plan elements based on the SOC, 'Manageability' is divided into 22 elements, 'Meaningfulness' is 11 and 'Comprehensibility' is 10 elements. Fourth, the details of the SOC items for each theory are as follows: BFD focuses on 'Manageability' with 'Manageability'(74%) and 'Comprehensibility'(26%), but 'Meaningfulness' does not exist. And UD regards 'Comprehensibility'(66%) as important, and 'Manageability' and 'Meaningfulness' as 17%. BD, on the other hand, has a high percentage of 'Meaningfulness'(70%), 'Comprehensibility'(21%) and 'Manageability'(9%). Next, the 'Manageability' of HD and EBD is 46%. And HD was 'Meaningfulness'(34%), 'Comprehensibility'(20%), and EBD was 'Meaningfulness'(30%) and 'Comprehensibility'(24%). The three items of SOC showed different distribution according to the spatial planning theory. As a result of the analysis, the spatial planning theory with the 'Comprehensibility' was related to Universal Design(UD), and the spatial planning theory with the 'Manageability' was related to Barrier-Free Design(BFD). In addition, the spatial planning theory of 'Meaningfulness' was related to Bio-philic Design(BD). Therefore, the plan of the elderly welfare center needs to approach the multidimensional design methodology to enhance the SOC(Sense of Coherence).

• Animal Bioscience
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• v.35 no.5
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• pp.721-729
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• 2022

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

• Tuberculosis and Respiratory Diseases
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• v.87 no.2
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• pp.165-175
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• 2024

Background: The prevalence of small airway dysfunction (SAD) in patients with chronic obstructive pulmonary disease (COPD) across different ethnicities is poorly understood. This study aimed to estimate the prevalence of SAD in stable COPD patients. Methods: We conducted a cross-sectional study of 196 consecutive stable COPD patients. We measured pre- and post-bronchodilator (BD) lung function and respiratory impedance. The severity of COPD and lung function abnormalities was graded in accordance with the Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines. SAD was defined as either difference in whole-breath resistance at 5 and 19 Hz > upper limit of normal or respiratory system reactance at 5 Hz < lower limit of normal. Results: The cohort consisted of 95.9% men, with an average age of 66.3 years. The mean forced expiratory volume 1 second (FEV₁) % predicted was 56.4%. The median COPD assessment test (CAT) scores were 14. The prevalence of post-BD SAD across the GOLD grades 1 to 4 was 14.3%, 51.1%, 91%, and 100%, respectively. The post-BD SAD and expiratory flow limitation at tidal breath (EFL_T) were present in 62.8% (95% confidence interval [CI], 56.1 to 69.9) and 28.1% (95% CI, 21.9 to 34.2), respectively. COPD patients with SAD had higher CAT scores (15.5 vs. 12.8, p<0.01); poor lung function (FEV₁% predicted 46.6% vs. 72.8%, p<0.01); lower diffusion capacity for CO (4.8 mmol/min/kPa vs. 5.6 mmol/min/kPa, p<0.01); hyperinflation (ratio of residual volume to total lung capacity % predicted: 159.7% vs. 129%, p<0.01), and shorter 6-minute walk distance (367.5 m vs. 390 m, p=0.02). Conclusion: SAD is present across all severities of COPD. The prevalence of SAD increases with disease severity. SAD is associated with poor lung function and higher symptom burden. Severe SAD is indicated by the presence of EFL_T.

• Journal of Broadcast Engineering
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• v.21 no.3
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• pp.349-356
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• 2016

In this paper, we propose an efficient assignment method that assigns quantization parameter (QP) in accordance with group of picture (GOP) structure given in HEVC encoder. Each video frames can have difference QP values based on given GOP configuration for HEVC encoding. Particularly, for important frames we can assign low QP values, and vice versa. However, there has not been thorough investigation on efficient QP assignment method by far. Even in HEVC reference software encoder, only monotonic QP assignment method is employed. Thus, the proposed method assign adaptive QP values to each GOP so that temporal dynamic activity between GOPs can be exploited. Through the experiment, the proposed method showed a 7.3% gain of compression performance in terms of BD-rate compared to HEVC test model (HM) in low-delay configuration, and outperformed the existing QP assignment study on average.

• Fashion & Textile Research Journal
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• v.21 no.3
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• pp.308-317
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• 2019

This study provided data for classifying and characterizing the lower half of the body shape for obese adult men in their 30s and 40s. Data of 492 adult males who were obese with a WHO criteria of >25 BMI were used for analysis. The results of the study are as follows. Six factors extracted from the factorial analysis as independent factors for cluster analysis were classified into three types. Type 1 (65.4%) had the lowest height of the lower half of the body with short circumference and length. Type 2 (20.3%) had the lowest height of the lower half of the body with the largest thickness, width and circumference from the back to the hip, but short in length. Type 3 (14.2%) had the lowest height of the lower half of the body with medium height and waist-height; however, the curve from the waist to the hip was the largest with the largest waist circumference, hip circumference, and width and thickness of the lower half of the body. This study will help to design a slack pattern that utilizes body shape characteristics of men in their 30s and 40s. In a follow-up study, we analyze the slack pattern and educational pattern by the company and study the necessity for a slack pattern for obese males.

• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.366-373
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• 2011

We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using two-dimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 ${\theta}/{\delta}$, and down-regulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ${\geq}2$ times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS-11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus' adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.345-352
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• 2009

This study utilized 332,625 records of linear type scores consisting for 15 primary traits, 22,175 final score and 84,612 pedigree information of 22,175 Holstein cows from 1993 to 2007 in Korea to estimate genetic parameters for 16 type traits. Genetic and error (co)variances between two traits selected from 16 traits were estimated using bi-trait pairwise analyses with DFREML package. The estimated heritabilities for stature (ST), strength (STR), body depth (BD), dairy form (DF), rump angle (RA), thurl width (TW), rear legs side view (RLSV), foot angle (FA), fore udder attachment (FUA), rear udder height (RUH), rear udder width (RUW), udder cleft (UC), udder depth (UD), front teat placement (FTP), front teat length (FTL) and final score (FS) were 0.31, 0.21, 0.25, 0.10, 0.29, 0.19, 0.09, 0.06, 0.12, 0.13, 0.12, 0.08, 0.26, 0.20, 0.28 and 0.15, respectively. ST had the highest positive genetic correlation with BD (0.90), while RLSV had the highest negative genetic correlation with FA (-0.56). RA had negative genetic correlation with most udder traits (-0.17~-0.02). Especially, RUW had the higher positive genetic correlation with STR (0.60), BD (0.62), and TW (0.49), however, UD had the higher negative genetic correlation with STR (-0.40) and BD (-0.40). FTL had negative genetic correlation with FUA, RUH, RUW, UC and UD. FS had positive genetic correlation with UC, UD and FTP (0.12, 0.18 and 0.20). However, additional research is needed on the use of these parameters in the genetic evaluation because estimated genetic and error variance-covariance matrices were not positive definite.

• Journal of Korea Multimedia Society
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• v.21 no.1
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• pp.18-25
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• 2018

In High Efficiency Video Coding (HEVC), integer motion estimation (IME) requires a large amount of computational complexity because HEVC adopts the high flexible and hierarchical coding structures. In order to reduce the computational complexity of IME, this paper proposes the search range reduction algorithm, which takes advantage of motion vectors similarity between different layers. It needs only a few modification for HEVC reference software. Based on the experimental results, the proposed algorithm reduces the processing time of IME by 28.1% on average, whereas its the $Bj{\emptyset}ntegaard$ delta bitrate (BD-BR) increase is 0.15% which is negligible.

• Food Science of Animal Resources
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• v.38 no.5
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• pp.950-958
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• 2018

The physicochemical characteristics and oxidative stability of wet-aged and dry-aged pork cuts were investigated at different aging periods (1, 7, 14 and 21 d). Samples were assigned into four groups in terms of shoulder blade-wet aging (SW), shoulder blade-dry aging (SD), belly-wet aging (BW), and belly-dry aging (BD). SD showed significantly higher pH at 21 d of aging than the other samples. Wet-aged cuts had significantly higher released water (RW) %, lightness ($L^*$) and shear force compared to the dry-aged meats. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed greater degradation of proteins for dry-aged cuts than the wet-aged cuts. At the end of aging, wet-aged cuts showed significantly lower thiobarbituric acid-reactive substances (TBARS) value than the dry-aged samples, indicating higher oxidative stability for wet-aged pork cuts. However, dry-aging led to higher degradation of proteins resulting in increased water-holding capacity (WHC) and decreased shear force value.