Gene Cloning, Purification, and Characterization of a Cold-Adapted Lipase Produced by Acinetobacter baumannii BD5
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Park, In-Hye
(Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
Kim, Sun-Hee (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) Lee, Yong-Seok (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) Lee, Sang-Cheol (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) Zhou, Yi (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) Kim, Cheol-Min (School of Medicine, College of Medicine, Pusan National University) Ahn, Soon-Cheol (School of Medicine, College of Medicine, Pusan National University) Choi, Yong-Lark (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) |
1 | Arpigny, J. L. and K. E. Jaeger. 1999. Bacterial lipolytic enzymes: Classification and properties. Biochem. J. 343: 177- 183 DOI ScienceOn |
2 | Han, S. J., J. H. Back, M. Y. Yoon, P. K. Shin, C. S. Cheong, M. H. Sung, S. P. Hong, I. Y. Chung, and Y. S. Han. 2003. Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01. Biochimie 85: 501- 510 DOI ScienceOn |
3 | Hong, M. C. and M. C. Chang. 1998. Purification and characterization of an alkaline lipase from a newly isolated Acinetobacter radioresistens CMC-1. Biotechnol. Lett. 20: 1027-1029 DOI ScienceOn |
4 | Jaeger, K. E. and T. Eggert. 2002. Lipases for biotechnology. Curr. Opin. Biotechnol. 13: 390-397 DOI ScienceOn |
5 | Kulakova, L., A. Galkin, T. Nakayama, T. Nishino, and N. Esaki. 2004. Cold-active esterase form Psychrobacter sp. Ant300: Gene cloning, characterization, and the effects of Gly→Pro substitution near the active site on its catalytic activity and stability. Biochim. Biophys. Acta 1696: 59-65 DOI PUBMED |
6 | Kumar, S., K. Kikon, A. Upadhyay, S. S. Kanwar, and R. Gupta. 2005. Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expr. Purif. 41: 38-44 DOI ScienceOn |
7 | Quyen, D. T., T T. G. Le, T. T. Nguyen, T. K. Oh, and J. K. Lee. 2005. High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1. Protein Expr. Purif. 39: 97-106 DOI ScienceOn |
8 | Ryu, H. S., H. K. Kim, W. C. Choi, M. H. Kim, S. Y. Park, N. S. Han, T. K. Oh, and J. K. Lee. 2006. New cold-adapted lipase from Photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases. Appl. Microbiol. Biotechnol. 70: 321-326 DOI ScienceOn |
9 | Snellman, E. A. and R. R. Colwell. 2004. Acinetobacter lipases: Molecular biology, biochemical properties and biotechnological potential. J. Ind. Microbiol. Biotechnol. 31: 391-400 DOI ScienceOn |
10 | Suzuki, T., T. Nakayama, D. W. Choo, Y. Hirano, T. Kurihara, T. Nishino, and N. Esaki. 2003. Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp. strain B11-1. Protein Expr. Purif. 30: 171-178 DOI ScienceOn |
11 | Kim, H. K., Y. J. Jung, W. C. Choi, H. S. Ryu, T. K. Oh, and J. K. Lee. 2004. Sequence-based approach to finding functional lipases from microbial genome databases. FEMS Microbiol. Lett. 235: 349-355 DOI PUBMED |
12 | Tang, S. J., K. H. Sun, G. H. Sun, T. Y. Chang, and G. C. Lee. 2000. Recombinant expression of the Candida rugosa lip4 lipase in Escherichia coli. Protein Expr. Purif. 20: 308-313 DOI ScienceOn |
13 | Kim, S. H., I. H. Park, S. C. Lee, Y. S. Lee, Y. Zhou, C. M. Kim, S. C. Ahn, and Y. L. Choi 2007. Discovery of the three novel lipase (lipA1, lipA2, and lipA3) and lipase-specific chaperone (lipB) genes present in Acinetobacter sp. DYL129. Appl. Microbiol. Biotechnol. 77: 1041-1051 PUBMED |
14 |
Breuil, C. and D. J. Kushner. 1974. Partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (Acinetobacter |
15 | Jaeger, K. E., B. W. Dijkstra, and M. T. Reetz. 1999. Bacterial, biocatalysts: Molecular biology, three-dimensional structures, and biotechnological applications of lipases. Annu. Rev. Microbiol. 53: 315-351 DOI ScienceOn |
16 | Sullivan, E. R., J. G. Leahy, and R. R. Colwell. 1999. Cloning and sequence analysis of the lipase and lipase chaperone encoding genes from Acinetobacter calcoaceticus RAG-1, and redefinition of a proteobacterial lipase family and an analogous lipase chaperone family. Gene 230: 277-285 DOI ScienceOn |
17 | Choo, D. W., T. Kurihara, T. Suzuki, K. Soda, and N. Esaki. 1998. A cold-adapted lipase of an alaskan psychrotroph, Pseudomonas sp. strain B11-1: Gene cloning and enzyme purification and characterization. Appl. Environ. Microbiol. 64: 486-491 PUBMED |
18 | Quyen, D. T., T. T. Nguyen, T. T. G. Le, H. K. Kim, T. K. Oh, and J. K. Lee. 2004. A novel lipase/chaperone pair from Ralstonia sp. M1: Analysis of the folding interaction and evidence for gene loss in R. solanacearum. Mol. Gen. Genomics 272: 538-549 DOI ScienceOn |
19 | Rashid, N., Y. Shimada, S. Ezaki, H. Atomi, and T. Imanaka. 2001. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A. Appl. Environ. Microbiol. 67: 4064-4069 DOI ScienceOn |
20 | Schlieben, N. H., K. Niefind, and D. Schomburg. 2004. Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus. Protein Expr. Purif. 34: 103-110 DOI ScienceOn |
21 | Snellman, E. A., E. R. Sullivan, and R. R. Colwell. 2002. Purification and properties of the extracellular lipase, LipA of Acinetobacter sp. RAG-1. Eur. J. Biochem. 269: 5771-5779 DOI ScienceOn |
22 | Zhang, J., S. Lin, and R. Zeng. 2007. Cloning, expression and characterization of a cold-adapted lipase gene from an Antarctic deep-sea psychrotrophic bacterium, Psychrobacter sp. 7195. J. Microbiol. Biotechnol. 17: 604-610 PUBMED |
23 | Kojima, Y., M. Kobayashi, and S. Shimizu. 2003. A novel lipase from Pseudomonas fluorescens HU380: Gene cloning, overproduction, renaturation-activation, two-step purification, and characterization. J. Biosci. Bioeng. 96: 242-249 DOI PUBMED |
24 | Luo, Y., Y. Zheng, Z. Jiang, Y. Ma, and D. Wei. 2006. A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification. Appl. Microbiol. Biotechnol. 73: 349- 355 DOI ScienceOn |
25 | Suzuki, T., T. Nakayama, T. Kurihara, T. Nishino, and N. Esaki. 2001. Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain No. 6. J. Biosci. Bioeng. 92: 144-148 DOI ScienceOn |
26 | Alquati, C., L. De Gioia, G. Santarossa, L. Alberghina, P. Fantucci, and M. Lotti. 2002. The cold-active lipase of Pseudomonas fragi. Eur. J. Biochem. 269: 3321-3328 DOI ScienceOn |
27 | Kok, R. G., J. J. Van Thor, I. M. Nugteren-Roodzant, M. B. Brouwer, M. R. Egmond, C. B. Nudel, B. Vosman, and K. J. Hellingwerf. 1995. Characterization of the extracellular lipase, LipA, of Acinetobacter calcoaceticus BD413 and sequence analysis of the cloned structural gene. Mol. Microbiol. 15: 803- 818 DOI ScienceOn |
28 | Bell, P. J. L., A. Sunna, M. D. Gibbs, N. C. Curach, H. Nevalainen, and P. L. Bergquist. 2002. Prospecting for novel lipase genes using PCR. Microbiology 148: 2283-2291 PUBMED ScienceOn |
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