• 대한예방한의학회지
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• 제18권2호
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• pp.115-123
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• 2014

Objective : The objective of this study is to develop a Qigong exercise program to reinforce musculoskeletal system of seniors appropriate for physical strength and conditions of seniors aged 65 years or above based on health Qigong exercise of oriental medicine. Method : Qigong exercise motions that can improve strength, muscular endurance, flexibility, and cardiovascular endurance of seniors were developed by primarily performing literature review of Qigong experts on the fields like oriental medicine, Qigong exercise, physical education and health science and secondarily using motion training for increased validity of motions. Results : The Qigong exercise program for musculoskeletal system of seniors was designed with 12 motions repeated 8 times and 20 minutes per session, including shoulder exercises (alternate turning of left and right shoulders, turning both arms back and forth, holding and lifting elbow to put it aside), waist exercises (wrapping the head with hands to bow, twisting waist while looking at the tip of hand, large spinning of ball, putting hands together to pull back), and knee exercises (going up a down while lifting a rock, balancing the body while lifting a knee, lifting and spreading knees while drawing circle with arms, raising both arms to the side while lifting heels, breathing). Conclusion : Once the effects of Qigong exercise for musculoskeletal system of seniors developed in this study are tested, the program is expected to contribute to development of Qigong exercise, a core part of oriental medicine health improvement project.

• 대한의생명과학회지
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• 제14권2호
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• pp.69-76
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• 2008

${\gamma}$-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system, and its actions are mediated by subtypes of GABA receptors named as $GABA_A$, $GABA_B,\;and\;GABA_C,\;GABA_A$, receptor consisting of ${\alpha},\;{\beta},\;{\gamma}\;and\;{\delta}$ subunits is a heterooligomeric ligand-gated chloride channel. This study was performed to investigate regulation of $GABA_A$ receptor by protein kinase C(PKC). Ion currents were recorded using gramicidine-perforated patch and whole cell patch clamp. mRNA encoding the subunits of PKC expressed in major pelvic ganglion (MPG) neurons was detected by using RT-PCR. The GABA-induced inward current was increased by PKC activators and decreased by PKC inhibitors, respectively. These effects were not associated with intracellular $Ca^{2+}$ and GAG (1-oleoyl-2-acetyl-sn-glycerol), a membrane permeable diacylglycerol (DAG) analogue. These results mean that the subfamily of PKC participating in activation of $GABA_A$ receptor would be an atypical PKC (aPKC). Among theses, ${\xi}$ isoform of aPKC was detected by RT-PCR. Taking together, we suggest that excitable $GABA_A$ receptor in sympathetic MPG neuron seemed to be regulated by aPKC, particular in ${\xi}$ isoform. The regulatory roles of PKC on excitatory $GABA_A$ receptors in sympathetic neurons of MPG may be an important factor to control the functional activity of various pelvic organs such as bowel movement, micturition and erection.

• The Korean Journal of Pain
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• 제22권3호
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• pp.206-209
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• 2009

Background: The essential oil of Ocimum basilicum (EOOB) has a pleasant aroma and is known to have antimicrobial and insecticidal activities. In addition, it is used as a pain reliever in folk medicine. However, there are few reports on the antinociceptive activities of EOOB. Methods: This study examined the antinociceptive effects of EOOB using formalin and a plantar test in mice. In the formalin test, EOOB (50 mg/kg, 100 mg/kg, 150 mg/kg) was administered intraperitoneally and the licking time of the mice was measured. In the plantar test, intraperitoneal EOOB (50 mg/kg, 100 mg/kg) was administered and the withdrawal latency was measured using the Hargreaves method. Results: In the formalin test, EOOB (50 mg/kg, IP) showed significant decreases in licking time in the second phase. On the other hand, in the plantar test, there were no significant effects in any of the groups examined. Conclusions: These results support the traditional use of EOOB for the treatment of painful conditions. However, there is a need for more research to determine the active chemical constituents and the precise mechanism.

• Journal of Korean Neurosurgical Society
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• 제49권1호
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• pp.1-7
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• 2011

Objective: Glutamate is a key excitatory neurotransmitter in the brain, and its excessive release plays a key role in the development of neuronal injury. In order to define the effect of nimodipine on glutamate release, we monitored extracellular glutamate release in real-time in a global ischemia rat model with eleven vessel occlusion. Methods: Twelve rats were randomly divided into two groups: the ischemia group and the nimodipine treatment group. The changes of extracellular glutamate level were measured using microdialysis amperometric biosensor, in coincident with cerebral blood flow (CBF) and electroencephalogram. Nimodipine (0.025 ${\mu}g$/100 gm/min) was infused into lateral to the CBF probe, during the ischemic period. Also, we performed Nissl staining method to assess the neuroprotective effect of nimodipine. Results: During the ischemic period, the mean maximum change in glutamate concentration was $133.22{\pm}2.57\;{\mu}M$ in the ischemia group and $75.42{\pm}4.22\;{\mu}M$ (p<0.001) in the group treated with nimodipine. The total amount of glutamate released was significantly different (P<0.001) between groups during the ischemic period. The %cell viability in hippocampus was $47.50{\pm}5.64$ (p<0.005) in ischemia group, compared with sham group. But, the %cell viability in nimodipine treatment group was $95.46{\pm}6.60$ in hippocampus (p<0.005). Conclusion: From the real-time monitoring and Nissl staining results, we suggest that the nimodipine treatment is responsible for the protection of the neuronal cell death through the suppression of extracellular glutamate release in the 11-VO global ischemia model of rat.

• 대한의생명과학회지
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• 제15권4호
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• pp.319-326
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• 2009

Extracellular ATP elicits diverse physiological effects by binding to the G-protein-coupled P2Y receptors on the plasma membrane. In addition to the short-term effects of extracellular nucleotides on cell functions, there is evidence that such purinergic signalling can have long-term effects on cell proliferation, differentiation and death. The 3T3-L1 cell line derived from mouse embryo is a well-established and commonly utilized in vitro model for adipocytes differentiation and function. However, the distributions and roles of P2Y subtypes are still unknown in the preadipocyte. In this study, we identified the distributions and roles of P2Y subtypes in preadipocyte using $Ca^{2+}$ imaging and realtime PCR. ATP increased the $[Ca^{2+}]_i$ in a concentration-dependent manner. ATP increased $Ca^{2+}$ in absence and/or presence of extracellular $Ca^{2+}$. Suramin, non-selective P2Y blocker, largely blocked the ATP-induced $Ca^{2+}$ response. U73122, a PLC inhibitor, completely inhibited $Ca^{2+}$ mobilization in 3T3-L1 cells. The mRNA expression by realtime PCR of P2Y subtypes was $P2Y_2:P2Y_5:P2Y_6=1.0:12.5:0.3$. In conclusion, we showed that $P2Y_5$ receptor is a dominant purinergic receptor in preadipocytes, and multiple P2Y receptors could involve in differentiation and migration via regulating of intracellular calcium concentration.

• Korean Journal of Acupuncture
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• 제28권4호
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• pp.101-112
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• 2011

Objectives : This study was done to investigate the degree of the pulse wave parameter of post-mastectomy women and to identify the relationships between each of them. Methods : Data were obtained from public health center in Busan Metropolitan city. Participants were 82 post-mastectomy women aged 31-82. the data collection was from Oct, 1, 2010 to Oct, 31, 2010. the data were analyzed with descriptive statistics, one-way ANOVA, Duncan test. A p-value less than .05 was considered significant by 2-tailed test. All statistical analyses were performed with SPSS/Win(ver 12.0). Results : As for the difference in the pulse wave by the general characteristics. The pulse energy was showed highly in the order of Chon, Quan and Chuk of left and right pulse respectively. In the pulse energy of left Quan and right Chon, the case of both mastectomy was showed highly and in the pulse energy of left Chuk, the case of right mastectomy was showed highly. Left and right pulse energy of menopause female were significantly higher than normal range except left Chon and Chuk. In h1, h2, h4, h5, t1, t2, AP and Aw, the pulse energy of left Chon was showed the largest significantly. In As, the pulse energy of right Chon was showed the smallest and in Ad, right Chuk was showed the largest. In RAI, right and left Chuk was showed the highest respectively. Conclusions : From these result, we can see that there are relation between women's age, area of operation breast and menopause after mastectomy in pulse wave. The result of this study will become basic data necessary for the Oriental Medicine treatment to reduce or prevent women's functional difficulties, symptomatology after mastectomy.

• 디지털융복합연구
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• 제14권3호
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• pp.427-436
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• 2016

본 연구는 정신장애인의 성경험에 대한 간호중재를 개발하기 위한 이론적 토대를 제공하고자 시도되었다. 사전동의서를 받고 정신장애인 14명의 인터뷰를 Strauss & Corbin(1998)의 근거이론방법에 의해 분석하였다. 정신장애인의 고립감, 성욕으로부터 탈출함의 만족도는 맥락인 대상자의 태도, 경제력 및 다스림과 중재상황인 파트너, 엮어짐 및 연속성에 따라서 둥지형, 절제형, 엉킴형의 3가지 유형이 나타났다. 따라서 정신장애인의 성경험은 자신의 몸이 존재하는 공간으로써의 현실을 인지하는 것으로 이들의 성문제에 대한 전문가 개입의 현실을 파악하여 앞으로 전문가역할을 활발히 하기 위한 방안을 모색해야 할 것이다.

• 한국학교ㆍ지역보건교육학회지
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• 제23권2호
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• pp.51-64
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• 2022

Objectives: This study aims to examine diabetic patients' quality of life by using the data of the 8th Korea National Health and Nutrition Examination Survey (1st year, 2019), identify the factors related to this, and utilize the results as basic data for intervention that can improve diabetic patients' quality of life. Methods: For the research subjects, this study extracted 624 patients who were diagnosed with Diabetes by a doctor from the total sample of 8,110 participants of the 8th Korea National Health and Nutrition Examination Survey. The SPSS(version25.0) program was used for the analysis of the collected data. Then, this study used a backward elimination multiple regression analysis method that applied complex sample, to examine the factors related with the finally estimated quality of life. Results: The results of this study revealed that diabetic patients' quality of life was related with gender, age, occupation, restriction of activity, subjected health status. The final model explained 35.7% of the variance (Wald F=28.210, p<.001). Conclusions: In order to improve the quality of life of diabetic patients, it would be desirable to provide differentiated management by developing a customized intervention strategy that takes into account gender, age, and occupation. When managing diabetic patients, the state, local governments, and hospitals should include content that prevents and copes with restrictions on activities that may occur due to disease. In addition, it is required to prepare a strategy to induce positive perception of the subject's own health status.

• Fisheries and Aquatic Sciences
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• 제26권3호
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• pp.188-203
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• 2023

Lysozymes are well-known antibacterial enzymes that mainly target the peptidoglycan layer of the bacterial cell wall. Animal lysozymes are mainly categorized as g-type, c-type, and i-type based on protein sequence and structural differences. In this study, c-type (AcLysC) and g-like-type (AcLysG-like) lysozymes from Amphiprion clarkii were characterized in silico via expressional and functional approaches. According to in silico analysis, open reading frames of AcLysC and AcLysG-like were 429 bp and 570 bp, respectively, encoding the corresponding polypeptide chains with 142 and 189 amino acids. Elevated expression levels of AcLysC and AcLysG-like were observed in the liver and the heart tissues, respectively, as evidenced by quantitative real-time polymerase chain reaction assays. AcLysC and AcLysG-like transcript levels were upregulated in gills, head kidney, and blood cells following experimental immune stimulation. Recombinant AcLysC exhibited potent lytic activity against Vibrio anguillarum, whereas recombinant AcLysG-like showed remarkable antibacterial activity against Vibrio harveyi and Streptococcus parauberis, which was further evidenced by scanning electron microscopic imaging of destructed bacterial cell walls. The findings of this study collectively suggest the potential roles of AcLysC and AcLysG-like in host immune defense.

• 한국병원경영학회지
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• 제28권2호
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• pp.21-31
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• 2023

Purposes: Patient experience is a tool to evaluate the process and results of medical services provided by medical institutions from the patient's point of view. Patient satisfaction surveys are a meaningful and essential source of information for improving quality in healthcare organizations. This study aims to provide basic data for improving the quality of medical service that patients can feel by analyzing the recommendation intention and satisfaction of inpatients in specialty hospitals. Methodology: The subjects of this study were 879 inpatients in 28 specialty hospitals in 14 designated fields. We conducted a telephone survey with a structured questionnaire on the satisfaction and recommendation intention for specialty hospitals. Findings: In inpatients, hospital satisfaction was higher in nursing care services and hospital satisfaction was low in physicians care services. The overall patient satisfaction score was 91.4(SD=11.9) out of 100, and the intention of recommendation was 92.0(SD=14.1) out of 100. The factors affecting patient experience were designated fields, sex, age, residential area, monthly household income, and perceived health status. Practical Implications: This study confirmed the high level of patient satisfaction and recommendation intention among inpatients of specialty hospitals. Patient satisfaction can be of great value to healthcare providers in recognizing and improving the quality of care, as well as predicting patients' willingness to revisit medical institutions. This study can be used to improve the quality of hospital care services in specialty hospitals rather than general and tertiary general hospitals.