• Title/Summary/Keyword: Basic Dye

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Structural Changes and Histochemical Study of Endosperm on Panax ginseng C.A. Meyer during Embryo Development (인삼(Panax ginseng C.A. Meyer) 종자의 배발달에 따른 배유의 구조변화 및 조직화학적 연구)

  • 유성철;김유갑
    • Journal of Ginseng Research
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    • v.16 no.1
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    • pp.37-43
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    • 1992
  • Structural changes of the endosperm of Panax ginseng C.A. Meyer from fertilization to germination were investigated by light microscope. The endosperm of the ginseng seed is cellular type. Since endosperm cells adjacent embryo continuously breakdown and disappear with the elongation of embryo, the real of endosperm is gradually decreased. As the anatropous ovules of immature seed with green seed coat developes more and more, ovary cells adjacent ovary cavity become abundant by the periclinal division, their size is decreased, hypotrophy of cell wall discern, and they are gradually differentiated in seed coat. Though embryo responds strongly to basic dye at the stage of completion of endosperm formation, tissue of endosperm responds to acidic dye positively Cell wall of embryo and endosperm are composed of primary cell wall not lignified. Endosperm cells adjacent embryo begin to breakdown in the endosperm tissue of indehiscent seed before the beginning of the after-ripening. Dehiscent seed of which seed coat is opened through after-ripening represent the form as a seedling in the result of embryo developments with the formation of organs; radicle, cotyledon, plumule. Umbilifom layer represents strong positive response to the toluidine blue and the basic function. Umbiliform layer that endosperm cells breakdown and disappear is observed clearly at the periphery of the embryo cotylemon, while slightly at the periphery of the radicle.

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Photoactivity of SnO2-Doped TiO2 Powder Sensitized with Quinacridone (Quinacridone을 첨가시킨 SnO2가 도핑된 TiO2 분말의 광촉매 특성)

  • Jung, Miewon;Kwak, Yunjung
    • Applied Chemistry for Engineering
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    • v.18 no.6
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    • pp.650-653
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    • 2007
  • $SnO_2$-doped $TiO_2$ powder was obtained from tin (IV) bis(acetylacetonate) dichloride and titanium diisopropoxide bis(acetylacetonate) with quinacridone as the dye sensitizer molecule. The structural changes of the reaction mixture were monitored by fourier transform infrared (FT-IR) spectroscopy. The morphology and microstructure of gel powder were studied by field-emission scanning electron microscopy (FE-SEM) and X-ray diffractometry (XRD). The photocatalytic activity of these powders with the anatase structure was investigated by using indigo carmine solution as a test dye

FRET-Based Quantitative Discrimination of Bisulfite-Untreated DNA from Bisulfite-Treated DNA

  • Lee, Eun Jeong;Cho, Yea Seul;Song, Seongeun;Hwang, Sang-Hyun;Hah, Sang Soo
    • Bulletin of the Korean Chemical Society
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    • v.35 no.5
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    • pp.1455-1459
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    • 2014
  • We report a sensitive and reliable FRET-based nanotechnology assay for efficient detection and quantification of bisulfite-unmodified or modified DNA. Bisulfite-untreated DNA or bisulfite-treated DNA is subjected to PCR amplification with biotin-conjugated primers so that the amounts of bisulfite-untreated and treated DNA can be differentiated. Streptavidin-coated quantum dots (QDs) are used to capture biotinylated PCR products intercalated with SYBR Green, enabling FRET measurement. Key features of our method include its low intrinsic background noise, high resolution, and high sensitivity, enabling detection of as little as 1.75 ng of bisulfite-untreated DNA in the presence of an approximately 1,000-fold excess of bisulfite-untreated DNA compared to bisulfate-treated DNA, with the use of PCR reduced (as low as 15 cycles). SYBR Green as an intercalating dye as well as a FRET acceptor allows for a single-step preparation without the need for primers or probes to be chemically conjugated to an organic fluorophore. Multiple acceptors per FRET donor significantly enhance the signal-to-noise ratio as well. In consideration of the high relevance of bisulfite treatment to DNA methylation quantitation, our system for FRET measurement between QDs and intercalating dyes can be generally utilized to analyze DNA methylation and to potentially benefit the scientific and clinical community.

Investigation of Fluorescent Shape Memory Polyurethanes Grafted with Various Dyes

  • Chung, Yong-Chan;Choi, Jae-Won;Lee, Seung-Hwan;Chun, Byoung-Chul
    • Bulletin of the Korean Chemical Society
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    • v.32 no.spc8
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    • pp.2988-2996
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    • 2011
  • Shape memory polyurethane (SMPU), grafted with a fluorescent dye (Rhodamine, Mehylene violet, or Fluorescein) through an allophanate linking, was tested for the fluorescence and the shape recovery effect. The main chain of SMPU was composed of 4,4'-methylenebis(phenylisocyanate) (MDI), poly(tetramethyleneglycol) (PTMG), and 1,4-butanediol (BD), and a fluorescent dye was connected through a second MDI linked to the carbamate moiety of the main chain. Three series of SMPU, differing according to their dye content, were prepared to compare their shape recovery and fluorescence properties. In tensile mechanical property, maximum stress increased up to 350% compared to the linear SMPU, and strain remained above 2000%. Shape recovery went to as high as 97%, and remained almost same after repetitive shape recovery test cycles. Finally, the fluorescence emission of SMPU was demonstrated in the luminescence spectrum and fluorescent light emission pictures. In addition, the response of SMPU to external stimuli such as metal ions was investigated.

Utilization of Cotton Stalks-Biomass Waste in the Production of Carbon Adsorbents by KOH Activation for Removal of Dye-Contaminated Water

  • Fathy, Nady A.;Girgis, Badie S.;Khalil, Lila B.;Farah, Joseph Y.
    • Carbon letters
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    • v.11 no.3
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    • pp.224-234
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    • 2010
  • Four activated carbons were produced by two-stage process as followings; semi-carbonization of indigenous biomass waste, i.e. cotton stalks, followed by chemical activation with KOH under various activation temperatures and chemical ratios of KOH to semi-carbonized cotton stalks (CCS). The surface area, total pore volume and average pore diameter were evaluated by $N_2$-adsorption at 77 K. The surface morphology and oxygen functional groups were determined by SEM and FTIR, respectively. Batch equilibrium and kinetic studies were carried out by using a basic dye, methylene blue as a probe molecule to evaluate the adsorption capacity and mechanism over the produced carbons. The obtained activated carbon (CCS-1K800) exhibited highly microporous structure with high surface area of 950 $m^2/g$, total pore volume of 0.423 $cm^3/g$ and average pore diameter of 17.8 ${\AA}$. The isotherm data fitted well to the Langmuir isotherm with monolayer adsorption capacity of 222 mg/g for CCS-1K800. The kinetic data obtained at different concentrations were analyzed using a pseudo-first-order, pseudo-second-order and intraparticle diffusion equations. The pseudo-second-order model fitted better for kinetic removal of MB dye. The results indicate that such laboratory carbons could be employed as low cost alternative to commercial carbons in wastewater treatment.

A Study on the Dye Wastewater Treatment Using TiO2 Photocatalyst/Ozonation (광촉매/오존을 이용한 염색폐수처리에 관한 연구)

  • Kim, Chang-Kyun;Chung, Ho-Jin;Kim, Jong-Suk
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.6
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    • pp.663-670
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    • 2007
  • This study was performed to provide basic information for evaluating the efficiency and applicable extent of photocatalysis and ozonation for the treatment of dye wastewater. The treatability of dye wastewater by $UV/TiO_2$ and $UV/TiO_2/O_3$ advanced oxidation process (AOP) was investigated under various conditions. The experiments were conducted in a batch reactor of 50 liters equipped with twelve UV Lamps of 16W. In $UV/TiO_2$ AOP, the removal efficiency of TCODMn and Color increased to 58% and 67% respectively with increasing UV intensity. Also, The removal efficiency of TCODMn and Color increased to 97% and 99% respectively with increasing $H_2O_2$. Acid area was more efficient than neutral and alkalic areas in wastewater treatment, and pH 5 was the most effective and the treatment efficiency continually increased as the amount of photocatalyst was increased. When the photocatalyst was increased, TCODMn was removed faster than Color.

In Situ Crosslinked Ionic Gel Polymer Electrolytes for Dye Sensitized Solar Cells

  • Shim, Hyo-Jin;Kim, Dong-Wook;Lee, Chang-Jin;Kang, Yong-Ku;Suh, Dong-Hack
    • Macromolecular Research
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    • v.16 no.5
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    • pp.424-428
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    • 2008
  • We prepared an ionic gel polymer electrolyte for dye-sensitized solar cells (DSSCs) without leakage problem. Triiodide compound (BTDI) was synthesized by the reaction of benzene tricarbonyl trichloride with diethylene glycol monotosylate and subsequent substitution of tosylate by iodide using NaI. Bisimidazole was prepared by the reaction of imidazole with the triethylene glycol ditosylate under strongly basic condition provided by NaH. BTDI and bisimidazole dissolved in an ionic liquid were injected into the cells and permeated into the $TiO_2$ nanopores. In situ crosslinking was then carried out by heating to form a network structure of poly(imidazolium iodide), thereby converting the ionic liquid electrolytes to a gel or a quasi-solid state. A monomer (BTDI and bisimidazole) concentration in the electrolytes of as low as 30 wt% was sufficient to form a stable gel type electrolyte. The DSSCs based on the gel polymer electrolytes showed a power conversion efficiency of as high as 1.15% with a short circuit current density of $5.69\;mAcm^{-2}$, an open circuit voltage of 0.525 V, and a fill factor of 0.43.

A Study on the Color of Natural Solvent for the Red Color Reproduction of Safflower

  • Lee, Mi Young;Wi, Koang Chul
    • Journal of Conservation Science
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    • v.37 no.1
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    • pp.13-24
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    • 2021
  • Safflower, a natural dye representing red, is the dye that materials and dyeing method are recorded in the literature, including materials and dyeing. Although the safflower is the same, the ash used as a mordant is recorded differently in each literature, which greatly affects the aesthetic perspective in realizing the traditional safflower red. Therefore, the optimal conditions for realizing the traditional safflower red were sought. The experiment was conducted by pH investigation, dyeing and color analysis by dyeing solution water, concentration, and temperature by ash, and the unique color of red was confirmed. As a result of the test, the pH point of time when the uniq ue color was expressed was 11.53 as goosefoot ash (natural bedrock water), which was 1:100 for concentration and 70℃ for temperature, which was easier to extract red pigment than other ash, indicating that it is suitable for safflower dyeing. The analysis of the ash showed that K and Si play an important role in dyeing, especially Si, which is an element that inhibits carthamon. The color of red was similar to that of KS Standard vivid purplish red in the ash of the goosefoot, and the other ash was deep purplish pink. In the light of findings, it was possible to quantify the dyeing method through traditional materials and find the standard color of red color, and it is judged to be a basic data for studying the unique color of natural materials.

Evaluation of Cell Viability and Delivery Efficiency in Electroporation System According to the Concentrations of Propidium Iodide and Yo-Pro-1 (전기천공시스템에서 Propidium Iodide와 Yo-Pro-1의 농도에 따른 세포 생존율과 전달효율 평가)

  • Bae, Seo Jun;Im, Do Jin
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.898-906
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    • 2019
  • In this study, basic research was conducted to provide guidelines for selecting fluorescent dye and using proper concentration of fluorescent dye to use evaluation of cell viability and fluorescent dye delivery efficiency. Propidium iodide and Yo-Pro-1 were used as fluorescent dyes. In the evaluation of cell viability and the efficiency of delivery using Propidium Iodide and Yo-Pro-1, the histogram of each fluorescent dye was different depending on the type of fluorescent dye and the concentration used. These results were related to the characteristics of the fluorescent dyes used. This was related to the properties of the fluorescent dyes used. From these results, it was found that the analytical results depending on the characteristics of the fluorescent dyes used in the cell analysis. The effect of the fluorescent dye on the cell was confirmed, but it was confirmed that it did not affect the analysis result. In addition, the influence of interference between fluorescent signals was confirmed when two or more kinds of fluorescent dyes were used for analysis. The higher the concentration of Yo-Pro-1 was, the larger the effect of interference was, and the concentration of Propidium Iodide did not affect the interference of fluorescence signal. This study confirmed that the evaluation of the cell viability and the evaluation of the delivery efficiency were influenced by the type and concentration of the fluorescent dyes and it was related to the characteristics of the fluorescent dyes. Based on the results, appropriate concentrations of fluorescent dyes suitable for evaluation of cell viability and delivery efficiency were suggested.

Antimicrobial Activity and Physical Properties of Acrylic Acid Grafted Nylon 6 (아크릴산 그라프트 Nylon 6의 항균성 및 물성)

  • 김수미;송화순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.5
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    • pp.686-695
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    • 2000
  • Recently, with a growing interest of health and environment, chitosan which was good in no harmful effect to human body and environment, has been watched as the finish treatment of hygiene and pleasantness. The purpose of this study is to develop multifunctional fabric that was improved antimicrobial activity and deodorization rate, water absorption, static voltage and dye absorption by treatment of nylon 6 grafted with acrylic acid added in chitosan. FT-IR spectra of the grafted with acrylic acid added in chitosan shows peaks of COOH and NH2. Antimicrobial activity and deodorization rate of chitosan and grafted with acrylic acid added in chitosan were increased greatly than the control, durability of laundry of grafted with acrylic acid added in chitosan was good. In case of chitosan was dyed acid dyes and grafted with acrylic acid added in chitosan was dyed basic dyes, dye absorption of them were increased than the control. Moisture regain, absorption time and tensile strength of grafted with acrylic acid added in chitosan was increased greatly than the control.

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