• 디지털융복합연구
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• 제15권8호
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• pp.501-509
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• 2017

본 연구는 과훈련에 따른 선수의 인지정보처리와 정서변화에 관련된 국내 외 문헌들을 고찰하여 과훈련 시 유발되는 신체적 피로감이 어떻게 선수들의 인지정보처리와 정서변화에 미치는 영향을 신경생리학적으로 연구할 필요성을 제시하고, 스포츠 현장에서 활동하고 있는 선수와 코치들에게 뇌 생리적 관점에서 과훈련 증상에 대한 이해를 높일 수 있는 기초자료를 제공하는데 있다. 전자데이터베이스를 이용하여 최근 20년간 발행된 국내외 학술자료 및 연구보고서 등을 검토하였으며, 검색된 학술 문헌 중 본 연구의 목적에 부합되는 문헌들을 중심으로 검토하였다. 검토한 문헌을 바탕으로 과훈련에 따른 선수의 인지정보처리와 정서변화에 대하여 검토한 결과, 신체의 피로감이 과훈련을 통해 누적되어 운동수행 저하뿐만 아니라 선수들의 인지 및 정서적 능력 측면에서도 문제가 발생될 수 있다는 것을 알 수 있었다. 그리고 ERP를 이용한 P300의 진폭과 잠재기 및 EEG를 이용한 대뇌반구비대칭 차이지표를 이용한 접근은 과훈련이 인지정보처리과정과 정서적 변화에 미치는 효과를 뇌생리심리적으로 이해시킬 수 있는 새로운 연구방법의 가능성도 확인하였다.

• 대한방사성의약품학회지
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• 제7권2호
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• pp.105-111
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• 2021

⁸⁹Zr is a positron-emitting radioisotope, which has known as well-suited radioisotope for use in a monoclonal antibody-based imaging agent for immuno-PET. The purpose of this study was to quantitatively evaluate the diagnostic ability of general trastuzumab and thio-trastuzumab as HER2 positive receptors based on Df hexadentate iron chelator. Desferrioxamine-p-SCN (Df-Bz-NCS) and desferroixamine-maleimide (Df-Mal) were purchased from Macrocyclics (Dallas, TX, USA). The trastuzumab was purchased from Roche (Schweiz), and thio-trastuzumab was obtained from professor Hyo-Jeong Hong group (Kangwon National University). The radioisotope ⁸⁹Zr was produced by domestic purification system and KIRAMS using medical cyclotron (50 MeV, Scantronix). The conjugates of Df-trastuzumab and Df-thio-trastuzumab were prepared with Df-Bz-NCS and Df-Mal under basic aqueous solution (pH 8-9) at room temperature, respectively. The conjugates purified by PD-10 column were mixed with dried ⁸⁹Zr chloride. ⁸⁹Zr-labeled conjugates were purified and concentrated by Amicon ultra centrifugal filter. The preparation step and time of ⁸⁹Zr-labeled conjugates was shorted as 4 steps within 2 hours. ⁸⁹Zr-labeled conjugates showed the highly radiochemical purity of over 98%, and were very stable until 7 days by the analysis of radio-ITLC method. Each radio-labeled conjugates were also exhibited the highly stability in both PBS buffer and mouse serum. Immuno-PET imaging of ⁸⁹Zr-labeled conjugates in mice bearing gastric cancer xenograft tumors with HER2 expression showed high tumor uptake in the NCI-N87 HER2-expressing. However, ⁸⁹Zr-Df-Mal-thio-trastuzumab showed a relatively lower tumor-to-background ratio than ⁸⁹Zr-Df-Bz-trastuzumab, as well as whole-body distribution. In the results, ⁸⁹Zr-Df-Bz-trastuzumab was evaluated to have a relatively higher HER2 diagnostic ability than ⁸⁹Zr-Df-Mal-thio-trastuzumab.

• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.26-34
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• 2009

Porcine embryonic stem (ES) cells have a great potential as tools for transgenic animal production and studies of regulation of differentiation genes. Although several studies showed successful derivation of porcine ES-like cells, these cells were not maintained long-term in culture. Therefore, this study was conducted to establish porcine pluripotent ES-like cells using in vivo fertilized embryos and to maintain these cells in long term culture. Porcine ES-like cells from in vivo embryos obtained by immunosurgery or whole explant culture were successfully cultured for over 56 passages. Morphology of porcine ES-like cells was flat-shaped with a monolayer type colony. These cells stained for alkaline phosphatase throughout the culture. Furthermore, porcine ES-like cells reacted with antibodies against Oct-4, SSEA-1, SSEA-4, Tra-1-60, and Tra-1-81, which are typical markers of undifferentiated stem cells. To characterize the ability of porcine ES-like cells to differentiate into three germ layers, embryoid body formation was induced. After plating of these cells, porcine ES-like cells were spontaneously differentiated into various cell types of all three germ layers. In addition, porcine ES-like cells were successfully derived from IVF blastocysts in media containing human recombinant basic fibroblast growth factor.

• 디지털융복합연구
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• 제13권2호
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• pp.393-399
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• 2015

오늘날은 가히 웰빙과 LOHAS 시대를 넘어 힐링시대라 할 만큼 모두들 스스로의 치료(self therapy)에 관심이 많다. 이에 따라 민간요법과 관련된 분야의 활발한 산업화 그리고 질병치료가 아닌 건강증진 등에 대한 관심이 증대되면서 다양한 분야에서 대체의학이나 대체요법에 대한 연구가 수행되고 있다. 이렇듯 민간요법을 통한 건강증진 및 인간의 자연치유력에 대한 관심이 증대되고 있는 시점에서, 민간요법에 대한 인식을 구성하는 요인들을 탐색해 보는 것은 매우 의미 있는 일이다. 이에 본 연구에서는 선행연구를 토대로 인식 속성 관련 설문문항을 개발하여 요인분석 등을 이용하여 민간요법을 구성하는 요인에 대해 탐색해 보고, 인구통계학적인 특성들에 따라 인식 속성에 어떠한 차이가 있는지를 통계적 검정해 보았다. 그 결과 24개로 구성된 민간요법 관련 측정 변수들은 4개의 요인, 즉, 건강증진요인, 안전요인, 심리요인 그리고 대안요인 등으로 분류되었다. 그리고 전체적으로 30세 이하의 젊은 층보다는 40~60대의 중장년층 그리고 학력이 높을수록 민간요법에 대한 사용경험이 높으며, 성별로는 큰 차이가 없음을 알 수 있었다.

• 한국환경보건학회지
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• 제46권3호
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• pp.344-352
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• 2020

Objectives: Much attention has been paid to indoor air quality. Ventilation within schools is important because of indoor air quality and its effect on health and learning performance. In this study, we evaluated the carbon dioxide (CO₂) concentrations and ventilation rates in schools. Methods: This study measured the concentration of CO₂ in elementary, middle, and high school classrooms over six months. The seasons during the study were summer, fall, and winter. Sensor-based monitoring was used and the basic characteristics of the classroom were investigated. The body surface area of the students was used to calculate the CO₂ generation rate, and the air change per hour (ACH) was evaluated using mass balance modeling. Results: The average CO₂ concentration measured in most schools exceeded 1000 ppm. The ventilation rates varied from season to season. Compared to the recommended ventilation rate of 4.9 ACH, the roughly 3 ACH calculated in this study indicates that most schools possessed insufficient ventilation. Conclusions: The concentration of CO₂ in school classrooms could be an indicator of indoor air quality and can affect students' learning ability. In this study, CO₂ concentrations exceeding the standard indicate a lack of ventilation along with problems with indoor air quality. Therefore, appropriate improvements are needed to overcome these problems.

• 지역사회간호학회지
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• 제5권2호
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• pp.203-215
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• 1994

The purpose of this study was to investigate the needs for exercise of adult inpatients and ultimately to provide basic data for planning nursing intervention of patients who need exercise. The tool used for this study was a structured questionnaire which consisted of 42 items. The test items were classified into seven factors. The seven factors of the needs for exercise were need for achievement, need for activity, need for affiliation, need for autonomy, need for catharsis, need for homeostasis, and need for exhibition. Subjects of this study were 127 patients in two general hospitals in Seoul, and one general hospital in Chun Ann City. The needs of exercise were identified and the data were analyzed by gender, age, occupation, education level, monthly income, size of patient room, patient group who requires exercise and the other patient group who requires bed rest, using one-way ANOVA and Scheffe test as post-hoc. The findings of this study were as follows ; 1) Among the needs for exercise of adult patients, the highest level of the needs was the need for homeostasis. The next highest was the need for achievement, then need for activity, need for catharsis, need for autonomy, need for affiliation, and the lowest was the need for exhibition. 2) Maintaining physical fitness stood first in the need for homeostasis, relieving psychological and physical powerlessness in the need for activity, mood diversion in the need for catharsis, happiness of the family in the need for affiliation, maintaining the balanced body figure in the need for exhibition, practicing planned life in the need for achievement, and improving the ability to overcome the crisis in the need for autonomy. 3) Male patients exhibited the higher level of the needs than female patients in all factors except the need for exhibition and homeostasis. 4) There was no statistical difference in the needs between age, occupation, monthly income, and the size of patient room. 5) The need for achievement was high as the level of education goes higher. 6) The patients who performed regular exercise before admission had higher needs for activity, achievement, and autonomy, compared with the patients who did not perform the regular exercise before admission. 7) There was no statistical difference in the needs between patient group who requires exercise and the other patient group who requires bed rest. The need for homeostasis was the highest in both groups. The results from this study suggest that the need for exercise of adult inpatients should be assessed before planning exercise intervention.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1499-1506
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• 2016

Background: Crocus sativus and its major constituent crocin are well established to have anti-cancer properties in breast cancer cells (MCF-7). However the role of C. sativus extract (CSE) and crocin on caspase signaling mediated MCF-7 cell death at molecular level is remains unclear. In this study, we tried to unravel role of CSE and crocin on caspase mediated MCF-7 cells death and their in vivo preclinical toxicity profiling and immune stimulatory effect. Materials and Methods: CSE extract was fractionated by HPLC and crocin was isolated and characterized by NMR, IR, and MS. MCF-7 cells were treated with both CSE and crocin and expression of Bcl-2 and Bax was assessed after 24 and 36 hours. Furthermore, caspase 3, caspase 8 and caspase 9 expression was determined by Western blotting after 24 hours of treatment. DNA fragmentation analysis was performed for genotoxicity of CSE and crocin in MCF-7 cells. The in vivo toxicity profile of CSE (300 mg/kg of b.wt) was investigated in normal Swiss albino mice. In addition, peritoneal macrophages were collected from crocin (1, 1.5 and 2 mg/kg body weight) treated mice and analyzed for ex vivo yeast phagocytosis. Results: Immunoblot analysis revealed that there was time dependent decline in anti-apoptotic Bcl-2 with simultaneous upregulation of Bax in CSE and crocin treated MCF-7 cells. Further CSE and crocin treatment downregulated caspase 8 and 9 and cleaved the caspase 3 after 24 hours. Both CSE and crocin elicited considerable DNA damage in MCF-7 cells at each concentration tested. In vivo toxicity profile by histological studies revealed no observable histopathologic differences in the liver, kidney, spleen, lungs and heart in CSE treated and untreated groups. Crocin treatment elicited significant dose and time dependent ex vivo yeast phagocytosis by peritoneal macrophages. Conclusions: Our study delineated involvement of pro-apoptotic and caspase mediated MCF-7 cell death by CSE and crocin at the molecular level accompanied with extensive DNA damage. Further we found that normal swiss albino mice can tolerate the maximum dose of CSE. Crocin enhanced ex vivo macrophage yeast phagocytic ability.

• Journal of Ginseng Research
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• 제30권1호
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• pp.41-48
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• 2006

우리나라의 건강기능식품법에서는 인삼 또는 홍삼의 기능성이 자양강장, 면역증진 및 원기회복으로 한정되어 있다. 지금까지 홍삼에 함유된 갈변물질과 산성다당체에 대해서 많은 연구가 수행되었는데, 갈변물질은 항산화 활성과 면역증진기능, 그리고 산성다당체는 소화기관암의 증상개선, 면역기능 증진, 혈압개선, 혈액응고방지, 조혈기능, 알콜해독(간기능 회복, 고지혈 개선 등) 등의 효능이 우수하다고 보고되었다. 이러한 관점에서 현재 인삼과 홍삼의 3가지 기능성외에 항산화활성이라든지 그 외 더 많은 기능성을 추가할 필요성이 있으며, 또 홍삼에 함유되어 있는 활성성분을 적극적으로 이용하여 기준규격형제품은 물론이고 질병위험감소에 대해서 기능성을 표시할 수 있는 개별인정형 제품이나 나아가서는 일반 또는 전문의약품으로 개발하여 홍삼의 이용 가능성을 극대화시키고 국제적으로 경쟁력이 있는 한국인삼으로 그 위상을 제고시켜야 한다고 생각한다.

• 디지털융복합연구
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• 제18권8호
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• pp.511-518
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• 2020

본 연구의 목적은 유아기 위험상황에서 자신의 신체를 안전하게 보호할 수 있는 안전보건 능력의 중요성이 강조되고 있지만 유아안전보건지식 측정에 대한 타당화된 검사도구의 부족으로, 유아의 안전보건지식 측정할 수 있는 도구 개발 필요성에 대한 근거를 마련하고자 하는 것이다. 연구목적을 달성하기 위하여 교육전문가 및 유아교육전문가의 의견을 수렴하여 만 3~5세의 유아 안전보건지식 측정에 적합한 구성요인을 추출하였다. 이를 기초로 개발된 측정문항은 3-5세 유아 186명을 대상으로 설문조사하여 문항구성의 적합도와 내용의 신뢰도를 검증하였다. 문항반응분포시 0.8이하값은 삭제하였고, 상관도 r<.40이하의 문항도 삭제하였고, 신뢰도검사에서 .70이상으로 나타났다. 탐색적 요인분석을 거쳐 총 3영역 23개 문항으로 개발되었다. 최종문항에 대한 안전보건지식 총점과 상관도는 .80이상으로 나타나 유의미한 상관이 있는 것으로 나타났다. 본 연구에서 개발된 유아안전보건지식 측정도구는 문항변별도, 문항신뢰도인 문항내적 일치도, 내용타당도는 적절한 것으로 확인되어 유아안전보건지식 측정을 위한 기초자료로 활용될 것이며 추후 더욱 타당화된 검증을 위하여 유아안전보건교육 프로그램 실시후 안전보건지식 향상의 효과를 검증하는 후속연구가 진행되어야 할 것이다.