• Title/Summary/Keyword: Base region

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Phylogenetic Analysis of Dendropanax morbifera Using Nuclear Ribosomal DNA Internal Transcribed Spacer (ITS) Region Sequences (Internal transcribed spacer (ITS) region의 염기서열 분석에 의한 보길도산 황칠나무의 분자 계통학적 연구)

  • Shin, Yong Kook
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1341-1344
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    • 2016
  • Dendropanax morbifera is an endemic tree species of Korea, it is restricted to the southern parts of Korea. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) for Dendropanax morbifera grown at Bogil-do, Korea was determined. We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. The determined sequences consisted of 689 residues. ITS1 was 222 bp long while ITS2 was 233 bp long. The 5.8S rDNA was 160 bp long. The ITS region sequences of the Dendropanax species included in this study were obtained from GenBank. Oreopanax polycephalus was used as the outgroup. A pairwise alignment was calculated using the Clustal X program. A phylogenetic tree was constructed by the neighbor-joining method using the Tree view program. Sequence similarities among species including D. morbifera Bogil-do isolate showed the range 92.6 to 99.7% in sequence-based phylogenetic analysis using total 615 base pairs of ITS1, 5.8S rDNA and ITS2. D. morbifera Bogil-do isolate exhibited the highest degree of relatedness to D. chevalieri, sharing 99.7% ITS region similarity. D. morbifera Bogil-do isolate also showed to D. trifidus, sharing 99.4% ITS region similarity.

Region Query Reconstruction Method Using Trie-Structured Quad Tree in USN Middleware (USN 미들웨어에서 트라이 구조 쿼드 트리를 이용한 영역 질의 재구성 기법)

  • Cho, Sook-Kyoung;Jeong, Mi-Young;Jung, Hyun-Meen;Kim, Jong-Hoon
    • Journal of Korea Spatial Information System Society
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    • v.10 no.1
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    • pp.15-28
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    • 2008
  • In ubiquitous sensor networks(USN) environment, it is essential to process region query for user-demand services. Using R-tree is a preferred technique to process region query for in-network query environment. In USN environment, USN middleware must select sensors that transfers region query with accuracy because the lifetime of sensors is that of whole sensor networks. When R-tree is used, however, it blindly passes the region query including non-existent sensors where MBR(Minimum Boundary Rectangle) of R-tree is Intersected by region of query. To solve in this problem, we propose a reconstruction of region query method which is a trie-structured Quad tree in the base station that includes sensors in region of query select with accuracy. We observed that the proposed method delays response time than R-tree, but is useful for reducing communication cost and energy consumption.

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Anterior Cranial Base Reconstruction in Complex Craniomaxillofacial Trauma: An Algorithmic Approach and Single-Surgeon's Experience

  • Shakir, Sameer;Card, Elizabeth B.;Kimia, Rotem;Greives, Matthew R.;Nguyen, Phuong D.
    • Archives of Plastic Surgery
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    • v.49 no.2
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    • pp.174-183
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    • 2022
  • Management of traumatic skull base fractures and associated complications pose a unique reconstructive challenge. The goals of skull base reconstruction include structural support for the brain and orbit, separation of the central nervous system from the aerodigestive tract, volume to decrease dead space, and restoration of the three-dimensional appearance of the face and cranium with bone and soft tissues. An open bicoronal approach is the most commonly used technique for craniofacial disassembly of the bifrontal region, with evacuation of intracranial hemorrhage and dural repair performed prior to reconstruction. Depending on the defect size and underlying patient and operative factors, reconstruction may involve bony reconstruction using autografts, allografts, or prosthetics in addition to soft tissue reconstruction using vascularized local or distant tissues. The vast majority of traumatic anterior cranial fossa (ACF) injuries resulting in smaller defects of the cranial base itself can be successfully reconstructed using local pedicled pericranial or galeal flaps. Compared with historical nonvascularized ACF reconstructive options, vascularized reconstruction using pericranial and/or galeal flaps has decreased the rate of cerebrospinal fluid (CSF) leak from 25 to 6.5%. We review the existing literature on this uncommon entity and present our case series of n = 6 patients undergoing traumatic reconstruction of the ACF at an urban Level 1 trauma center from 2016 to 2018. There were no postoperative CSF leaks, mucoceles, episodes of meningitis, or deaths during the study follow-up period. In conclusion, use of pericranial, galeal, and free flaps, as indicated, can provide reliable and durable reconstruction of a wide variety of injuries.

Effects of Base Changes at the Transcription Start Site on Stringent Control of rnpB in Escherichia coli

  • Choi, Hyun-Sook;Park, Jeong Won;Hong, Soon Kang;Lee, Kangseok;Lee, Younghoon
    • Molecules and Cells
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    • v.26 no.2
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    • pp.212-215
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    • 2008
  • The GC-rich discriminator sequence between the -10 region and the transcription start site of the rnpB promoter is responsible for stringent control of M1 RNA synthesis. The rnpB promoter also contains a G nucleotide at the previously identified transcription start site. In this study, we examined by mutagenesis of G to A whether this +1G nucleotide is involved in the stringent response. We found that the change did not alter the stringent response. Since the +1 mutation might alter transcription initiation, we compared the transcription start sites of the wt and mutant promoters by primer extension analysis. Surprisingly, we found that wild type rnpB transcription starts at both the +1G position (70%) and the -1C position (30%), and that the +1A mutation led to transcription initiation exclusively at the -1C position. We also generated two transversion mutations at the -1 position, both of which led to transcription starting exclusively at that position. The -1G mutant promoter gave a stringent signal similar to the wild-type, whereas the -1A mutant generated a significantly less stringent signal. Base on these results, we propose that a short sequence, up to 7 bp downstream of the -10 region, is involved in the stringent response of the rnpB promoter.

Cooperative Management Framework for the Transboundary Coastal Area in the Western Part of Korean Peninsula (서해연안 접경지역 현황 및 남북한 협력관리 방안)

  • Nam, Jung-Ho;Kang, Dae-Seok
    • Journal of Environmental Policy
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    • v.3 no.2
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    • pp.1-29
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    • 2004
  • As a result of very limited access due to the military confrontation between South and North Koreas for the last five decades, ecosystems in the transboundary coastal area in the western part of Korean Peninsula have been protected from intensive developments in both Koreas. In the core of the recent two military collisions lies the fishery resources represented as blue crabs as well as the politico-military aspect. Increasing development pressures from both sides as reflected in the South Korea supporting the construction of an industrial complex in Kaesung, North Korea, is the main factor which threatens the sustainable resource base in this region. This research is aimed to develop a cooperative management system for the well-preserved transboundary coastal area between South Korea and North Korea. The Pressure-State-Response (PSR) framework of OECD was used to assess environmental conditions, socioeconomic pressures on the environment of the region, and policy responses of both Koreas to those pressures. Protection of ecosystems, peace settlement, and prosperity of the region and the entire peninsula were proposed as the management goals of the cooperative management system. The designation of the area as a Co-managed Marine Protected Area System (COMPAS) through close cooperation among South Korea, North Korea, and international entities was suggested as a way to achieve those goals. Revision of legal and institutional mechanisms, strengthening knowledge base for optimal COMPAS management, integration of the marine protected area and DMZ (demilitarized zone) ecosystem, enhancing stakeholder participation, building international partnership, and securing financial resources were presented as six management strategies.

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DNAse 1 Hypersensitive Sites of Lung Specific Transcription Factor Gene (폐특이 전사조절 유전자의 DNAse 1 Hypersensitive Sites)

  • Lee, Yong-Chul
    • Tuberculosis and Respiratory Diseases
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    • v.48 no.6
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    • pp.879-886
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    • 2000
  • Background : Thyroid Transcription Factor-1(TTF-1) acts as a tissue specific transcription factor in the regulation of lung specific gene expression and as morphogenic protein during lung organogenesis. Currently, there is very little information on the cis-acting sequences and transcription factors that direct the TTF-1 gene expression. DNAse 1 hypersensitive (DH) sites represent a marker for active or potentially active chromatin and are likely to be especially important in gene regulation, being associated with many DNA sequences that regulate gene expression. It is clear that DH regions correlate with genetic regulatory loci and binding for sequence-specific DNA-binding proteins. Methods : We have used DH site assays to identify putative distal regulatory elements in H441 lung adenocarcinoma cells, which express the TTF-1 gene and HeLa cells. Results : There are four DH sites 5' of the TTF-1 gene. These sites are located at base pair approximately +150, -450, -800, and -1500 from the start of transcription. Conclusion : These data suggest that there may be at least one intragenic site and regulatory region 5' prime to the promotor region.

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Estimation of Site Index Equation for Pinus densiflora at Mt. Osu Region using Schnute Growth Function (Schnute 생장함수를 이용한 오서산지역 소나무림의 지위지수 추정)

  • Pyo, Jung-Kee;Lee, Young-Jin;Son, Yeong-Mo;Lee, Kyeong-Hak;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.43 no.4
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    • pp.9-14
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    • 2009
  • The purpose of this study was to estimate height growth pattern and to derive site index equation for Pinus densiflora grown at Mt. Osu region, Chungnam. A total of 21 repre - sentative trees from the established temporary plots around Boryeng and Cheongyang regions were destructively sampled to measure diameter, height, volume growth per 1-year interval by using of DTRS- 2000 measurement instrument. The Schnute growth function was select ed for height growth prediction using 181 height-age paired observations and derived anamorphic base age invariant site index curves (base index age 30 years). The fitted index and the asymptotic value of Schnute model indicated both 0.96 and 10.8 meter, respectively. The results suggested in this study could be very useful to understand the height growth pattern and productivity of the site quality on Pinus densiflora grown at Mt. Osu region, Chungnam.

Projection of Potential Cultivation Region of Satsuma Mandarin and 'Shiranuhi' Mandarin Hybrid Based on RCP 8.5 Emission Scenario (RCP 8.5 기후변화시나리오에 근거한 온주밀감과 '부지화'의 잠재적 재배지 변화 예측)

  • Moon, Young-Eel;Kang, Seok-Beom;Lee, Hyejin;Choi, Young-Hun;Son, In-Chang;Lee, Dong-Hoon;Kim, Sung-Ki;An, Moon-Il
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.19 no.4
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    • pp.215-222
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    • 2017
  • The potential change of the cultivation area of main citrus cultivars, satsuma mandarin (Citrus unshiu Marc.) and 'Shiranuhi' mandarin hybrid [(Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata] were determined with base year (1981 to 2010) to 2090. The meteorological data provided by the Korea Meteorological Administration (KMA), and the digital agricultural climate map of 30m-solution based on the Representative Concentration Pathways (RCP) 8.5 was used for projection of potential cultivation area. As a result, the potential suitable region of satsuma mandarin included almost Jeju region during base year. At the 2030s, the potential suitable region of satsuma mandarin increased and the cultivable region also increased focused on the coast region of Jeonnam province. From the 2060s, the suitable area spread out to mountain area of Jeju, Jeonnam, Gyeongnam, and the coast region of Kangwon, and the cultivable region expanded to the area of Gyeongbuk, Chungnam, and Jeonbuk. In the case of 'Shiranuhi' mandarin hybrid, the suitable region included only the partial coast area of Jeju, and cultivable area covered Jeju region and the partial southern coast of Jeonnam during the standard period. At the 2030s, the suitable region of 'Shiranuhi' included the current cultivation area of satsuma mandarin, and the cultivable region moved to northward by the partial southern coast region. At the 2090s, the slightly increased suitable region covered all Jeju regions, Jeonnam, Gyeongnam, and the coast area of Kangwon, and the cultivable region proceeded northward focusing on the coastline. In conclusion, the prediction of the potential land for citrus cultivation based on the RCP 8.5 showed that the suitable region of satsuma mandarin decreased, whereas that of cultivation of 'Shiranuhi' increased. Moreover, it was forecasted that citrus cultivation area would extend to Kangwon region at the end of the $21^{st}$ century.

Implementation of 500BASE-T with 2 Pairs UTP (2조 UTP를 이용한 500BASE-T의 구현)

  • Chung, Hae;Jeon, Seong-Bae;Kim, Jin-Hee;Park, Hyung-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.10B
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    • pp.1150-1158
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    • 2011
  • More than 100 Mbps rate is needed in the UBcN for a subscriber to receive broadband traffics with multi-channel like UDTV or 3DTV. Although the optical fiber is recently deployed for the FTTH, the UTP is the most widely used medium and will be used in UBcN age. Network providers may consider the 1000BASE-T or the vectorized VDSL if they adopts the UTP in the place where does not have optical fibers. But UTP should be expanded because 1000BASE-T and vectorized UTP needs 4 and 3 pairs cable, respectively while residential region has not exceeding 2 pair UTP cable. To solve the problem, we propose a 500BASE-T technology using 2 pairs UTP in this paper. The technology introduces a rate adaptation sublayer and a SERDES sublayer above and under the PCS, respectively. The rate adaptation sublayer is compatible for the GMII. Also, if we modify the SERDES sublayer, the technology can easily obtain 250BASE-T with 2 pairs UTP. We implement such functions with FPGA and analog board and verify the function of rate adaptation and symbol vector synchronization, and effective transmission rate by experiments. In particular, we show that link efficiency is increased by enable control in the rate adaptation sublayer.

Genetic Screening of the Canine Transcription Factor AP-2 Beta(TFAP2B) Gene in Dogs with Patent Ductus Arteriosus(PDA) (동맥관 개존증(PDA)에 이환된 개에서의 전사 인자 AP-2 beta(TFAP2B) 유전자 스크리닝)

  • Nam, So-Jeong;Hyun, Chang-Baig
    • Journal of Veterinary Clinics
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    • v.26 no.2
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    • pp.123-129
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    • 2009
  • Patent ductus arteriosus(PDA) is an abnormal shunt between the descending aorta and pulmonary artery through the incompletely closed ductus arteriosus and is the most common congenital heart defect in dogs. Recent human genetic studies found that a the gene mutation in transcription factor AP-2 beta(TFAP2B) was responsible for syndromic cases of PDA. Mutations in the TFAP2B gene are associated with certain congenital cardiac defects in humans that include PDA. In this study, we isolated the entire coding exons of canine TFAP2B gene for genetic screening in dogs with PDA. Analysis of the deduced amino acid sequence suggested that the canine TFAP2B are phylogenetically closer to the human TFAP2B(100% identity in amino acid sequence) than mouse and rat. In cTFAP2B gene screening, one single c.936+203G>A base change was found in affected Maltese dogs with PDA. However, further screening found the same base change in one unaffected control dog, suggesting this base change might be polymorphism. No other base changes were found in other dog breeds enrolled in this study. Because the base change was located in the intronic region and found in an unaffected control dog, TFAP2B might not be responsible for familial PDA in Malteses and sporadic cases of other dog breeds, although the gene promoter region should be investigated before reaching to this conclusion. A future study that may take this study further would be to collect more samples and to screen TFAP2B in various breeds of dogs with PDA and other various congenital heart defects.