• Title/Summary/Keyword: Barley

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Comparison of Liquid and Solid-State Fermentation Processes for the Production of Enzymes and Beta-Glucan from Hulled Barley

  • Lee, Se Yeon;Ra, Chae Hun
    • Journal of Microbiology and Biotechnology
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    • v.32 no.3
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    • pp.317-323
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    • 2022
  • Solid-state fermentation using hulled barley was carried out to produce enzymes and β-glucan. The one-factor-at-a-time experiments were carried out to determine the optimal composition of the basal medium. The modified synthetic medium composition in liquid-state fermentation was determined to be 70 g/l hulled barley, 0 g/l rice bran, 5 g/l soytone, and 6 g/l ascorbic acid. Optimal pretreatment conditions of hulled barley by solid-state fermentation were evaluated in terms of maximum production of fungal biomass, amylase, protease, and β-glucan, which were 1.26 mg/g, 31310.34 U/g, 2614.95 U/g, and 14.6% (w/w), respectively, at 60 min of pretreatment condition. Thus, the solid-state fermentation process was found to enhance the overall fermentation yields of hulled barley to produce high amounts of enzymes and β-glucan.

Special References on Status and Prospects of Seed Quality Researches in Wheat and Barley (맥류품질 연구 현황과 전망)

  • Ha, Yong-Woong;Lee, Eun-Sup;Nam, Jung-Hyun;Song, Hyeon-Suk;Hong, Byeong-Hee;Cho, Chang-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.s01
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    • pp.18-38
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    • 1988
  • The purpose of this report is to suggest researchers the future directions for studing barley and wheat quality by reviewing the researches that have been reported on the qualities of them until now as well as by discussing the future prospects in it; to summarize 196 reports, however, is so difficult in many respects that some of them were refered in this review, and only the subjects of all the reports were listed in the reference instead.

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Inheritance of Waxy and Fractured Starch Endosperm of Barley (보리 찰성 및 분장성 전분의 유전)

  • Nam, Jung-Hyun;Lee, Eun-Sup;Chung, Tae-Young;Park, Moon-Woong;Cho, Chang-Hwan;Shim, Jae-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.1
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    • pp.16-18
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    • 1986
  • This experiment was conducted to know the genetic nature of waxy and fractured starch endosperm genes in 1983. F$_2$ seeds involve simple recessive gene(1:3) for the waxy and fractured starch endosperm genes, respectively. Also, association between waxy and fractured starch endosperm have shown to be segregated as expected to fit in the ratio 9:3:3:1 respectively of normal-nonwaxy: fractured-nonwaxy: normal-waxy: fractured-waxy showing the acceptable value of X$^2$ test of independence.

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Effect of Continual Application of Liquid Pig Manure on Malting Barley Growth and Soil Environment in Double Cropping System of Rice-Malting Barley (벼.맥주보리 작부체계에서 돈분액비 연용이 맥주보리 생육과 토양 환경에 미치는 영향)

  • Lee, Seong-Tae;Seo, Dong-Cheol;Kim, Eun-Seok;Song, Won-Doo;Lee, Won-Gyu;Heo, Jong-Soo;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.3
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    • pp.341-348
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    • 2010
  • To investigate the effect of continual pre-plant application of liquid pig manure (LPM) on malting barley growth, quality and soil environment in double cropping system of rice and malting barley, the liquid pig manure was applied after harvesting rice and malting barley for 3 years. Field experiment was designed with non-fertilizer, chemical fertilizer (CF) recommended by soil testing, rice (LPM 50%+CF 50%)+malting barley (CF 100%), rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%), rice (LPM 100%)+malting barley (CF 100%) and rice (LPM 100%)+malting barley (LPM 100%). By continuous application of LPM 100%, the contents of available $P_2O_5$ and exchangeable K in the soil were increased. The available $P_2O_5$ increased from 243 to 350 mg $kg^{-1}$ and exchangeable K was changed 0.31 to 0.44 $cmol_{c}\;kg^{-1}$. However, the contents of available $P_2O_5$ and exchangeable K were not significant changes in rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%) plot. Bulk density of soil was not affected by application of LPM. The microbial density was high in order of bacteria > actinomycetes > fungi. The population of aerobic bacteria in rice (LPM 100%)+malting barley (LPM 100%) plot was higher than other plots. The ratio of aerobic bacteria/fungi and biomass C content were the highest in rice (LPM 100%)+malting barley (LPM 100%) plot. The yield of malting barley was increased 22% by increasing culm length, panicle length, No. of panicle and 1,000 grains as 358 kg $10a^{-1}$ in rice (LPM 100%)+malting barley (LPM 100%) plot compared with 294 kg $10a^{-1}$ in rice (CF 100%)+malting barley (CF 100%) plot. The content of ${\beta}$-glucan was low by 4.5 and 4.4% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot, respectively. The content of crude protein was the lowest by 8.2% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot and the quality of malting barley was good as within 11%.

Effects of Barley Straw Management Practices on Greenhouse Gases(GHGs) Emission During Rice Cultivation in Rice-barley Double Cropping System (벼보리 이모작 재배에서 보리짚 처리 방법이 벼재배시 온실가스 배출에 미치는 영향)

  • Ko, Jee-Yeon;Lee, Jae-Saeng;Jung, Ki-yul;Choi, Young-Dae;Ramos, Edwin P;Yun, Eul-Soo;Kang, Hwang-Won;Park, Seong-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.1
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    • pp.65-73
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    • 2008
  • Because main barley straw management is changing these days from off-fields to burning that may relate to air quality concerning the global warming, this study was conducted to investigate the effects of barley-straw management practices on greenhouse gas emissions during rice cultivation in rice-barley double cropping system. The treatments were barley straw burning, off-field usage of barley straw and incorporation of barley straw in paddy fields. Laboratory experiment showed that burning of barley straw at the rate of $4.5Mg\;ha^{-1}$ emitted GHGs in the amounts of 4,607, 19.5, and $0.9kg\;ha^{-1}$ of $CO_2$, $CH_4$, and $N_2O$, respectively. During the rice cultivation of the rice-barley double cropping system, the highest GHG emission by evaluated close-static chamber method was observed from the soil incorporation of barley straw with 387 and $1.0kg\;ha^{-1}$ of $CH_4$ and $N_2O$, respectively. The GHGs emissions from the barley straw burning and off-field usage treatments were 233 and $160kg\;ha^{-1}$ for $CH_4$ and 0.80 and $0.79kg\;ha^{-1}$ for $N_2O$, respectively. The barley straw burning treatment showed the greatest GHGs emission among barley straw management practices in rice-barley double cropping system when considering GHGs emissions both during burning and from paddy fields during the cropping seasons. As a result, the GHGs emissions recorded in the barley straw incorporation to soil and off-field usage treatments were 22.4 and 66.8%, respectively, less than sum of GHGs emissions from the burning of barley straw and from paddy fields during rice cultivation.

Biochemical and Genetic Variation of Hordein Subunits in Korean Barley

  • Lee, Sung-Shin;Kim, Jin-Baek;Kim, Dong-Sub;Nam, Jung-Hyun;Kim, Jae-Chul;Kim, Chung-Kon;Hong, Byung-Hee;Seo, Yong-Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.100-105
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    • 2001
  • One-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used to determine whether it would provide improved resolving power of hordein proteins concomitant with improved identification of Korean barley cultivars and germplams. This system gave rapid and reproducible separations of hordein polypeptides. Total fourteen of clear and easily scorable subunits were identified in Korean barley cultivars and germplasms and their polymorphic constitutions could provide biochemical genetic information in progeny analysis and endosperm quality improvement in barley breeding programs. Each hordein polypeptides residing in B, C, and D hordein pattern designations were scored to prepare a cultivar catalogue of protein patterns. On the basis of this character, 7 hordein polypeptide patterns were constructed from 108 barley cultivars and experimental lines. The molecular weight of hordein subunits in Korean barley cultivars and experimental lines varied in the range of 98 to 48 kDa. In contrast, less polymorphic hordein polypeptides were found in the low protein barley lines including malting barleys than those found in Korean barley cultivars and experimental lines.

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Proteome Analysis of Paenibacillus polymyxa E681 Affected by Barley

  • Seul, Keyung-Jo;Park, Seung-Hwan;Ryu, Choong-Min;Lee, Yong-Hyun;Ghim, Sa-Youl
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.934-944
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    • 2007
  • Paenibacillus polymyxa E681 is known to be able to suppress plant diseases by producing antimicrobial compounds and to promote plant growth by producing phytohormones, and secreting diverse degrading enzymes. In spite of these capabilities, little is known regarding the flow of information from the bacterial strain to the barley roots. In an attempt to determine the flow of information from the bacterial strain to barley roots, the strain was grown in the presence and absence of barley, and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry were used. 2D-PAGE detected approximately 1,000 spots in the cell and 1,100 spots in the supernatant at a pH 4-10 gradient. Interestingly, about 80 spots from each sample showed quantitative variations. Fifty-three spots from these were analyzed by MALDI-TOF mass spectrometry and 28 proteins were identified. Most of the cytosolic proteins expressed at higher levels were found in P. polymyxa E681 cells grown in the presence of barley rather than in the absence of barley. Proteins detected at a lower level in the surpernatant of P. polymyxa E68l cells grown in the presence of barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase, heat-shock protein HtpG, spermidine synthase, OrfZ, ribonuclease PH, and coenzyme PQQ synthesis protein, and flagellar hook-associated protein 2 whereas proteins detected at a higher level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley included D-alanyl-D-alanine ligase A, isopentenyl-diphosphate delta-isomerase, ABC transporter ATP-binding protein Uup, lipase. Many of the proteins belonging to plant-induced stimulons are associated with biosynthetic metabolism and metabolites of proteins and transport. Some of these proteins would be expected to be induced by environmental changes resulting from the accumulation of plant-secreted substances.

A Study on Mechanized System of Barley Harvesting (보리의 기계수확체계(機械收穫體系) 시험(試驗))

  • Kim, Jeung Soo;Lee, Dong Hyeon;Baek, Poong Ki;Jeung, Doo Ho
    • Journal of Biosystems Engineering
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    • v.7 no.2
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    • pp.36-44
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    • 1983
  • Farm population was rapidly decreasing due to shift of the people from farm sector to the non-farm sector caused by the economic growth of the country. Especially, a great shortage of farm labor in busy farming period in June and October is becoming a serious problem in maintaining or promoting land productivity. The peak of labor requirement in summer is caused by rice transplanting and barley harvesting. In order to reduce the restrictions imposed on farm management by the concurrence of labor requirement and the lack of labor, the experimental study for mechanization of barley harvesting has been carried out in the fields. 1. The machines for barley harvesting were knap-sack type reapers, windrow reaper (power tiller attachment), binder and combine. The order of higher efficiency of machine for barley harvesting was combine, binder, windrow reaper (WR), knapsack type reaper 1(KSTR1), and knap sack type reaper 2(KSTR2; mist and duster attachment). 2. The ratio of grain loss for the manual, binder, and combine plot was about four percent of total field yield. 3. The total yield of barley in 35 days and 40 days harvesting after heading were 514 kg and 507kg per 10 ares respectively. The yield of 35 days-plot was higher than other experimental plots. 4. The lowest yield was recorded in 30 days-plot due to the large quantity of immatured grains and having lighter 1000-grain weight. The ratio of immatured grains was 2.66 percent and 1000-grain weight was 29.4 grams. 5. The total harvesting cost of the windrow reaper was 10,178 won per 10 ares. It was the lowest value compared to other machines. The next were combine, binder, KSTR1, KSTR2, and manual in sequence. As a result, the optimum time of barley harvesting for mechanization was 35-40 days after heading. Combine, binder, and windrow reaper were recommended as the suitable machines for barley harvesting in the work efficiency. However, in total harvesting cost, the windrow reaper was the most promising machine for barley harvesting.

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Serological detection of barley stripe mosaic virus infection in the seeds of barley and wheat cultivars grown in Korea (혈청학적방법에 의한 보리와 밀종자의 보리${\cdot}$줄무늬 모자이크 바이러스 감염상조사)

  • La Yong-Joon;Park Yang-Kyo
    • Korean journal of applied entomology
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    • v.18 no.1 s.38
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    • pp.29-33
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    • 1979
  • An attempt was made to find out whether barley stripe mosaic virus (BSMV) occurs in barley and wheat cultivars in Korea. Modified Hamilton's technique for serological detection of barley stripe mosaic virus was used to assay for BSMV in mature seed embryos. Of the 51 barley and wheat seed lots assayed, BSMV was detected in 34 of them. BSMV was detected in covered, naked, and matting barley as well as in wheat. The level of BSMV infection in the infected cultivars varied from $2\~35\%$. This is the first reported occurrence of barley stripe mosaic virus in Korea.

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Yield and Nitrogen Uptake under Reduced Nitrogen Fertilizer during Early Growth of Rice in the Rice-Barley Double Cropping System

  • Seo, Jong-Ho;Cho, Hyeon-Suk;Kim, Chung-Guk;Lee, Jin-Mo;Park, Seong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.1-6
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    • 2004
  • N fertilizer required by rice could be reduced greatly in the rice-barley double cropping system than in the rice single cropping system. This study was conducted to investigate how much of the N fertilizer during the early stage of rice in the rice-barley double cropping system, could be saved compared to that in the rice single cropping system. This experiment was carried out at the paddy field of the National Crop Experiment Station in Suwon, Korea during three years from 1999 to 2001. Amounts of soil mineral nitrogen (SMN) and SPAD values of rice leaf during rice growing season in the rice-barley double cropping system were higher than those in the rice single cropping system under the same amount of N application during two years. Yield and N uptakes of rice at harvesting time were also higher in the rice-barley double cropping system than in the rice single cropping system during two years. Yield and N uptake of rice in the rice single cropping system were decreased when basal N fertilizer was omitted, but those reductions were not found by either omitting basal N fertilizer or omitting N fertilizer at tillering stage in the rice-barley double cropping system during 2000 and 2001. But yield and N uptakes of rice were decreased by 70 kg/10a and 2kgN/10a by the omission of both N application at basal and tillering stages in the rice-barley double cropping system in 2002. It was concluded that N fertilizer as much as tillering N fertilizer could be saved in the rice-barley double cropping system.