• Korean Journal of Pharmacognosy
• /
• v.45 no.2
• /
• pp.181-185
• /
• 2014

This study was carried out to investigate the acute toxicity of the bark of Diospyros kaki (Ebenaceae) in mice. The aqueous extract of the bark of Diospyros kaki (AEDK) was administered orally at a doses of 5 mg/kg, 50 mg/kg, 300 mg/kg and 2,000 mg/kg. In this study, number of deaths, clinical signs, body weights and pathological examinations were investigated after administration of AEDK. There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and AEDK treated groups in clinical signs, organ weights and gross pathological findings. AEDK did not show any toxic effect in mice.

• Mycobiology
• /
• v.49 no.1
• /
• pp.54-60
• /
• 2021

During the screening of Korean microflora, a fungal strain (KNU-PT-1804) belonging to the genus Pestalotiopsis was isolated from persimmon tree (Diospyros kaki) bark collected from North Gyeongsang Province, Korea. The strain, KNU-PT-1804, produced smaller conidia compared with related species P. kenyana, P. neglecta, and P. telopeae. The novelty of the strain was confirmed based on phylogenetic analysis using molecular datasets of internal transcribed spacer (ITS) regions, β-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1α) genes. Molecular phylogeny strongly supports that the strain is distinct from previously known Pestalotiopsis species, and we proposed the novel species, Pestalotiopsis kaki sp. nov., and provide a detailed description and illustration.

• Journal of the Korean Wood Science and Technology
• /
• v.52 no.2
• /
• pp.191-203
• /
• 2024

Persimmon wood (Diospyros kaki) is a seasonal fruit-producing plant with a beautiful dark pattern in its wood that is suitable for high-quality furniture, sculptures and musical instruments. The utilization of persimmon wood can be improved by determining its anatomical characteristics, such as juvenile and mature wood. This study aimed to determine the boundaries between juvenile and mature wood and observe the anatomical properties of juvenile and mature wood and their variations in the axial direction. Three 30-year-old persimmon (D. kaki) trees grown in Karo, North Sumatra, Indonesia, were used in this study. The boundary between juvenile and mature wood was determined by measuring the fiber length and vessel element length from near the pith to near the bark. Anatomical observations were conducted in the juvenile and mature wood areas. The results showed that the average boundaries between juvenile and mature wood were 44.11 mm from the pith and were not significantly different in the axial direction of the trees. Furthermore, the wood anatomy categories of juvenile and mature wood differed significantly in terms of fiber diameter, fiber proportion, vessel proportion, and axial parenchyma proportion. In the axial direction, vessel diameter, ray parenchyma frequency, and ray parenchyma proportion at the base, middle, and top of the tree were significantly different.

• The Korean Journal of Mycology
• /
• v.47 no.1
• /
• pp.13-18
• /
• 2019

A fungal strain, designated PTT-2, was isolated from the bark of the trunk of a persimmon (Diospyros kaki) tree in Cheongdo, Korea. The isolate showed morphological similarities with Leptosphaerulina saccharicola. Strain PTT-2 had more rapid growth on potato dextrose agar medium than on oatmeal agar, malt extract agar, and synthetic nutrient poor agar media, with colony sizes of 53.8 mm, 49.8 mm, 48.4 mm, and 28.1 mm after 7 days at $25^{\circ}C$ temperature, respectively. Strain PTT-2 produced ascospores, which had irregular wavy edges, oblong to ellipsoidal shape, hyaline appearance and $23.6{\times}10{\mu}m$ size. The black ascomata were developed on PDA medium, and asci were recorded. A BLAST search of the internal transcribed spacer (ITS) region, TEF1-${\alpha}$ and RPB2 gene sequences revealed that strain PTT-2 showed more than 99% nucleotide similarity with a strain of Leptosphaerulina saccharicola previously reported from Thailand. A neighbor-joining phylogenetic tree was constructed by concatenating the above-mentioned sequences, and showed that strain PTT-2 clustered in the same clade with L. saccharicola. Based on these findings, this is the first record of Leptosphaerulina saccharicola occurring in Korea.