Browse > Article
http://dx.doi.org/10.1080/12298093.2020.1852703

Pestalotiopsis kaki sp. nov., a Novel Species Isolated from Persimmon Tree (Diospyros kaki) Bark in Korea  

Das, Kallol (College of Agriculture and Life Sciences, Kyungpook National University)
Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University)
Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Mycobiology / v.49, no.1, 2021 , pp. 54-60 More about this Journal
Abstract
During the screening of Korean microflora, a fungal strain (KNU-PT-1804) belonging to the genus Pestalotiopsis was isolated from persimmon tree (Diospyros kaki) bark collected from North Gyeongsang Province, Korea. The strain, KNU-PT-1804, produced smaller conidia compared with related species P. kenyana, P. neglecta, and P. telopeae. The novelty of the strain was confirmed based on phylogenetic analysis using molecular datasets of internal transcribed spacer (ITS) regions, β-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1α) genes. Molecular phylogeny strongly supports that the strain is distinct from previously known Pestalotiopsis species, and we proposed the novel species, Pestalotiopsis kaki sp. nov., and provide a detailed description and illustration.
Keywords
Novel species; persimmon; Pestalotiopsis kaki; phylogeny; taxonomy;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Senanayake IC, Maharachchikumbura SSN, Hyde KD, et al. Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Divers. 2015;73(1):73-72.   DOI
2 Steyaert RL. Contributions al'etude monographique de Pestalotia de Not. et Monochaetia Sacc. (Truncatella gen. nov. et Pestalotiopsis gen. nov.). Bull Jard Bot Natl Belg. 1949;19(3):285-354.
3 Das R, Chutia M, Das K, et al. Factors affecting sporulation of Pestalotiopsis disseminata causing grey blight disease of Persea bombycina Kost., the primary food plant of muga silkworm. Crop Prot. 2010;29(9):963-968.   DOI
4 Maharachchikumbura SSN, Guo LD, Chukeatirote E, et al. Pestalotiopsis-morphology, phylogeny, biochemistry and diversity. Fungal Divers. 2011;50(1):167-187.   DOI
5 Xu J, Ebada SS, Proksch P. Pestalotiopsis a highly creative genus: chemistry and bioactivity of secondary metabolites. Fungal Divers. 2010;44(1):15-31.   DOI
6 Kohlmeyer J, Kohlmeyer VB. Fungi on Juncus roemerianus 16. More new coelomycetes, including Tetranacriella gen. nov. Bot Mar. 2001;44:147-156.   DOI
7 Maharachchikumbura SSN, Hyde KD, Groenewald JZ, et al. Pestalotiopsis revisited. Stud Mycol. 2014;79:121-186.   DOI
8 Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118.   DOI
9 White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press, Inc.; 1990. p. 315-322.
10 O'Donnell K, Cigelnik E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol. 1997;7(1):103-116.   DOI
11 Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-120.   DOI
12 Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425.
13 Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981;17(6):368-376.   DOI
14 Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool. 1971;20(4):406-416.   DOI
15 Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874.   DOI
16 Jeewon R, Liew ECY, Hyde KD. Phylogenetic evaluation of species nomenclature of Pestalotiopsis in relation to host association. Fungal Divers. 2004;17:39-55.
17 Maharachchikumbura SSN, Chukeatirote E, Guo L-D, et al. Pestalotiopsis species associated with Camellia sinensis (tea). Mycotaxon. 2013;123(1):47-61.   DOI
18 Wei JG, Xu T, Guo LD, et al. Endophytic Pestalotiopsis species associated with plants of Podocarpaceae, Theaceae and Taxaceae in southern China. Fungal Divers. 2007;24:55-74.
19 Rehner SA. Primers for Elongation Factor 1 alpha (EF1 alpha). 2001. http://ocid.nacse.org/research/deephyphae/EF1primer.pdf.
20 Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330.   DOI
21 Gure A, Wahlstrom K, Stenlid J. Pathogenicity of seed-associated fungi to Podocarpus falcatus in vitro. Forest Pathol. 2005;35(1):23-35.   DOI
22 Trapero A, Romero MA, Varo R, et al. First report of Pestalotiopsis maculans causing necrotic leaf spots in nursery plants of Arbutus unedo and Ceratonia siliqua in Spain. Plant Dis. 2003;87(10):1263.   DOI
23 Jeon YH, Kim SG, Kim YH. First report on leaf blight of Lindera obtusiloba caused by Pestalotiopsis microspora in Korea. Plant Pathol. 2007;56(2):349-349.   DOI
24 Swart L, Taylor JE, Crous PW, et al. Pestaiotiopsis leaf spot disease of Proteaceae in Zimbabwe. S Afr J Bot. 1999;65(3):239-242.   DOI
25 Liu F, Hou L, Raza M, et al. Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China. Sci Rep. 2017;7(1):866.   DOI
26 Arzanlou M, Torbati M, Khodaei S, et al. Contribution to the knowledge of pestalotioid fungi of Iran. Mycosphere. 2012;3(5):871-878.   DOI
27 Liu F, Bonthond G, Groenewald JZ, et al. Sporocadaceae, a family of coelomycetous fungi with appendage-bearing conidia. Stud Mycol. 2019;92:287-415.   DOI