The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Leptosphaerulina saccharicola Isolated from Persimmon (Diospyros kaki) Tree Bark in Korea

  • Fulbert, Okouma Nguia (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Ayim, Benjamin Yaw (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Das, Kallol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lim, Yang-Sook (Agricultural Environment Research Department, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2019.01.30
  • Accepted : 2019.03.08
  • Published : 2019.03.01
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Abstract

A fungal strain, designated PTT-2, was isolated from the bark of the trunk of a persimmon (Diospyros kaki) tree in Cheongdo, Korea. The isolate showed morphological similarities with Leptosphaerulina saccharicola. Strain PTT-2 had more rapid growth on potato dextrose agar medium than on oatmeal agar, malt extract agar, and synthetic nutrient poor agar media, with colony sizes of 53.8 mm, 49.8 mm, 48.4 mm, and 28.1 mm after 7 days at $25^{\circ}C$ temperature, respectively. Strain PTT-2 produced ascospores, which had irregular wavy edges, oblong to ellipsoidal shape, hyaline appearance and $23.6{\times}10{\mu}m$ size. The black ascomata were developed on PDA medium, and asci were recorded. A BLAST search of the internal transcribed spacer (ITS) region, TEF1-${\alpha}$ and RPB2 gene sequences revealed that strain PTT-2 showed more than 99% nucleotide similarity with a strain of Leptosphaerulina saccharicola previously reported from Thailand. A neighbor-joining phylogenetic tree was constructed by concatenating the above-mentioned sequences, and showed that strain PTT-2 clustered in the same clade with L. saccharicola. Based on these findings, this is the first record of Leptosphaerulina saccharicola occurring in Korea.

Keywords

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Fig. 1. Cultural characteristics of Leptosphaerulina saccharicola PTT-2. A, B, colonies on potato dextrose agar; C, D, colonies on oatmeal agar; E, F colonies on malt extract agar; G, H colonies on synthetic nutrient poor agar (scale bar = 10 μm).

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Fig. 2. Morphological characteristics of Leptosphaerulina saccharicola PTT-2. A, asci; B, C, D, ascospores of PTT-2 (scale bar: A = 250 μm, B~D = 10 μm).

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Fig. 3. Neighbor-joining phylogenetic tree, based on the concatenated ITS, TEF1-α, LSU and RPB2 gene sequences shows the phylogenetic position of Leptosphaerulina saccharicola PTT-2 among members of the genus Leptosphaerulina. The strain isolated in this study was shown in boldface. Bootstrap values (based on 1,000 replications) are shown at the branch points. Botryosphaeria qingyuanensis CERC 2946 was used an outgroup.

Table 1. GenBank numbers of fungal strains used for phylogenetic analyses in this study

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Table 2. Comparison of morphological characteristics of isolate PTT-2 with reference to strains of Leptosphaerulina saccharicola

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