• Journal of Conservation Science
• /
• v.36 no.5
• /
• pp.326-332
• /
• 2020

The this study's objective was to conduct species identification and tree-ring dating for the wood elements of Daewoong-jeon(main temple), Hwagye-temple, Seoul, Korea. Most of the wood elements were made from Pinus spp.(hard pine). However, one large beam was constructed from Abies spp.. For the tree-ring dating, cores were taken from the elements using a drill, and ring-width plots of individual samples were produced with the TSAP software program. The tree-ring dating results showed that, the date of the outermost ring of most elements (20 elements), including beams and pillars, were from AD 1839 to 1870. Among them, five elements had bark, and felling dates were AD 1868 and 1869. These dates confirmed the historical records that Daewoongjeon was rebuilt in AD 1870 from the records of hidden materials(Bokjangmul) in Jongdori by the survey 2012.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.3
• /
• pp.301-305
• /
• 2016

Two newly recorded species of Melastomataceae, Medinilla rubicunda and M. septentrionalis, were found from the Phnom Bokor National Park and the Central Cardamom Protected Forest in Cambodia. Medinilla rubicunda is similar to M. laurifolia from Indonesia (Sumatra and Java) and Thailand, but is readily distinguished from the latter by its shorter petiole and a cyme bearing fewer than six flowers. Medinilla septentrionalis is similar to M. nana from China (Yunnan) and Vietnam, but is clearly distinguished from the latter by its terete branchlets, which have a thin and not a corky bark. Descriptions, photographs, and a diagnostic key of the three species of Cambodian Medinilla are provided for species identification.

• Journal of the Korean Wood Science and Technology
• /
• v.33 no.2 s.130
• /
• pp.56-64
• /
• 2005

This study was performed to investigate the frequency of blue staining fungal species collected from pine trees, Experimental Forests of Kangwon National University in Korea based on their morphological characteristics. In addition the tolerance to fungicide, Woodguard, was assessed to get basic knowledges for preventing blue stain of wood. Totally Leptographium-type fungi were dominated by 79.3% among Ophiostomatoid fungi associated with scolityd bark beetles in pine trees. Leptographium-type Ds-isolates which have unusual morphology were collected as frequency of 17.0%. The most distinct differeneces of these Ds-isolates from L. procerum were the presence of roughened hyphae and flask-shaped conidiophores that have never been mentioned formerly for L. procerum, but since these Ds-isolates formed black concentric rings being a property of L. procerum, the Df-isolates were characterized as Leptographium-type fungi, which are the most common species with the highest frequency by 33.2% in this particular area. According to our experimental results, Leptographium-type Ds- and Df-isolates were very resistant to fungicide, Woodguard, therefore it was suggested that a new method for wood protection from the blue staining fungi should be developed. Exact identification of blue staining isolates collected from pine trees is keep going.

• The Korean Journal of Food And Nutrition
• /
• v.12 no.3
• /
• pp.320-327
• /
• 1999

The flavonoids are one of the most numberous and widespread groups of natural consituents. The low molecular weight of benzo-${\gamma}$-pyrone derivative are ubiquitous in plants and are vegetables nuts, seeds, leaves, flowers, and bark. The flavonoids constitute of a large class of compounds ubiquitous in plants containing a number of phenolic hydroxyl groups attached to ring structures conferring the antioxidant activity. Epidemiologic studies suggest that the dietary intake of antioxidants constitutes a risk factor for vasclar disease indicating that oxidation may be important in the pathogenesis of human athero-sclerosis. Elevated plasma low density lipoprotein (LDL) cholesterol concentration are associated with accelerated atherosclerosis, LDL is oxidized by smooth muscle cells resulting in several chemicals and physical changes of LDL. Oxidized LDL is responsible for cholesterol loading of macrophages foam cells formation and atherogenesis. There have been insulficient tests of the protective effects of flavonoids against LDL oxidation to make definitive statements about their structure activity relationships. How-ever hydroxylation of the flavone uncleas can appears to be advantageous because polyhydroxylated aglycone flavonoids are potent inhibitor of LDL modification. This identification may lead to new and more effective antioxidant strategies for abrogating the atherosclerotic process the leading cause of death and disability in industrialized societies.

• Mycobiology
• /
• v.44 no.4
• /
• pp.202-216
• /
• 2016

Arthonia coreana, Arthonia superpallens, and Arthonia zelkovae are new species from South Korea. All new species are in the Euarthonia tribe, based on the key characteristics of colorless hypothecium and multi-cellular spores. A. coreana has a dull brownish hypophloedal thallus without bleaching and rounded or curved big apothecia in comparison with those of Arthonia punctiformis. A. coreana consistently exhibits 4-septate ascospores, which is a distinctive characteristic that distinguishes it from other Arthonia species. A. superpallens has a white-greenish thallus, pale yellowish apothecia, and a trentepohlioid alga. However, A. superpallens has no distinct prothallus, adnate, and convex apothecia, no pycnidia, and is UV-, in contrast with related species in the Arthonia antillarum group. A. zelkovae has a white, epiphloedal thallus, brownish-black epruinose apothecia covered with a whitish bark layer, and smaller ascospores in comparison with those of A. punctiformis. A. zelkovae consists of a chlorococcoid alga, which differs from related Arthonia species such as A. punctiformis, Arthonia pinastri, and Arthonia glaucella. Although A. zelkovae is similar to Arthonia dispersa in its white-colored thallus, blackish apothecia, and the presence of a chlorococcoid photobiont, A. zelkovae differs from the latter in having larger-sized 3-septate ascospores. Arthonia cinnabarina f. marginata, A. glaucella, Arthonia ilicinella, Arthonia lapidicola, Arthonia leioplacella, Arthonia pertabescens, A. pinastri, Arthonia spadicea, and Arthonia stellaris are newly described in Korea. The diagnostic characteristics of these species are discussed and presented. An artificial key is provided to facilitate identification of Arthonia species from Northeast Asia.

• The Korean Journal of Mycology
• /
• v.49 no.3
• /
• pp.315-324
• /
• 2021

In this study, the fungal strains KNU-Gunwi 2B and KNU-SOT5 were isolated from root-soil in a hillside and the cherry tree bark (Prunus serrulata), respectively from Gyeongbuk province in Korea. The strain KNU-SOT5 produced dark brown chlamydospores that were smooth to lightly rough-walled, globose to ellipsoidal, and the conidia were aseptate, guttulate, mostly fusiform with a diameter of 5.3-17.6×4.2-7.0 ㎛. Strain KNU-Gunwi 2B produced phialides that were smooth-walled, cylindrical with semi-papillate apices and the conidia were pale-green, broadly ellipsoid, and sometimes cylindrical with a diameter of 4.4-8.0×2.3-4.0 ㎛. The strain KNU-SOT5 and KNU-Gunwi 2B were resolved based on cultural and morphological characteristics, along with the phylogenetic analysis using the small subunit (SSU), large subunit (LSU), and internal transcribed spacers (ITS) regions. The fungal strains KNU-SOT5 and KNU-Gunwi 2B were identified as Dothidea insculpta and Metarhizium rileyi, which have not been reported in Korea.

• The Plant Pathology Journal
• /
• v.37 no.2
• /
• pp.124-136
• /
• 2021

Bark canker, wood discoloration, and wilting of the duku tree (Lansium domesticum) along the watershed of Komering River, South Sumatra Province, Indonesia first appeared in 2013. The incidence of tree mortality was 100% within 3 years in badly infected orchards. A Ceratocystis species was consistently isolated from the diseased tissue and identified by morphological and sequence analyses of the internal transcribed spacer (ITS) and β-tubulin regions. Pathogenicity tests were conducted and Koch's postulates were confirmed. The fungus was also pathogenic on Acacia mangium, but was less pathogenic on mango. Partial flooding was unfavourable for disease development. Two described isolates (WRC and WBC) had minor variation in morphology and DNA sequences, but the former exhibited a more pathogenic on both duku and acacia. The ITS phylogenies grouped the most pathogenic isolate (WRC) causing wilting of the duku tree within the aggressive and widely distributed ITS5 haplotype of C. fimbriata.

• Journal of the Korean Wood Science and Technology
• /
• v.31 no.1
• /
• pp.16-21
• /
• 2003

The methanol extractives from root bark of Ulmus davidiana var japonica nakai were fractionated with n-hexane, ethyl ether, ethyl acetate and waster, the water soluble fraction was also fractionated with silicagel column chromatograhy. The chemical structure of purifided compounds were identified with UV, IR, ¹H-NMR and ¹³C-NMR spectra and the antibacterial activities also were investigated. Two different antibacterial compounds (compound A and B) were fractionated with silicagel chromatography and TLC. Compounds B was identified as a catechin rahmnoside. The both of compounds had antibacterial activity on Staphylococcus aureus and Salmonella typhimurium.

• The Korean Journal of Mycology
• /
• v.50 no.4
• /
• pp.319-329
• /
• 2022

In July 2020, wilting symptoms were observed among adzuki bean plants (Vigna angularis var. angularis L.) in the fields in Yeosu, Korea. Infected plants showed yellowing of leaves, browning inside the stems, splitting of stem bark, and wilting. When these plants were uprooted, their roots were found to be brown. The fungal pathogens NC20-737, NC20-738, and NC20-739 were isolated from symptomatic stem and root tissues. These pathogens were identified as a Fusarium fujikuroi species complex based on their morphological characteristics. Molecular identification was performed using the DNA sequence of translation elongation factor 1 alpha and the RNA polymerase II second largest subunit regions. The nucleotide sequences of all three isolates were similar to the F. fujikuroi reference isolates NRRL 13566 and NRRL 5538 of the National Centre for Biotechnology Information GenBank. A pathogenicity test was conducted by the soil inoculation method with cornmeal sand inoculum. Approximately 3 weeks after inoculation, symptoms were observed only in the inoculated adzuki bean seedlings. To the best of our knowledge, this is the first report of Fusarium root rot caused by F. fujikuroi in adzuki beans, both in Korea and worldwide.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.23 no.1
• /
• pp.135-143
• /
• 2009

There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. Moutan Root Sark (MRS) has been frequently used as analgesic. antispasmodic, anti-inflammatory and remedies for female diseases. In this study. the antioxidant activity of extract from MRS was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The MRS extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the MRS extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.