The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification and Characterization of Ceratocystis fimbriata Causing Lethal Wilt on the Lansium Tree in Indonesia

  • Suwandi, Suwandi (Department of Plant Protection, Faculty of Agriculture, Sriwijaya University) ;
  • Irsan, Chandra (Department of Plant Protection, Faculty of Agriculture, Sriwijaya University) ;
  • Hamidson, Harman (Department of Plant Protection, Faculty of Agriculture, Sriwijaya University) ;
  • Umayah, Abu (Department of Plant Protection, Faculty of Agriculture, Sriwijaya University) ;
  • Asriyani, Khoirotun Dwi (Department of Plant Protection, Faculty of Agriculture, Sriwijaya University)
  • Received : 2020.08.05
  • Accepted : 2021.01.25
  • Published : 2021.04.01
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Abstract

Bark canker, wood discoloration, and wilting of the duku tree (Lansium domesticum) along the watershed of Komering River, South Sumatra Province, Indonesia first appeared in 2013. The incidence of tree mortality was 100% within 3 years in badly infected orchards. A Ceratocystis species was consistently isolated from the diseased tissue and identified by morphological and sequence analyses of the internal transcribed spacer (ITS) and β-tubulin regions. Pathogenicity tests were conducted and Koch's postulates were confirmed. The fungus was also pathogenic on Acacia mangium, but was less pathogenic on mango. Partial flooding was unfavourable for disease development. Two described isolates (WRC and WBC) had minor variation in morphology and DNA sequences, but the former exhibited a more pathogenic on both duku and acacia. The ITS phylogenies grouped the most pathogenic isolate (WRC) causing wilting of the duku tree within the aggressive and widely distributed ITS5 haplotype of C. fimbriata.

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References

  1. Al Adawi, A. O., Al Jabri, R. M., Deadman, M. L., Barnes, I., Wingfield, B. and Wingfield, M. J. 2013. The mango sudden decline pathogen, Ceratocystis manginecans, is vectored by Hypocryphalus mangiferae (Coleoptera: Scolytinae) in Oman. Eur. J. Plant Pathol. 135:243-251. https://doi.org/10.1007/s10658-012-0081-7
  2. Badan Pusat Statistik‒Statistics Indonesia. 2018. Statistics of annual fruit and vegetable plants Indonesia 2017. URL https://www.bps.go.id/publication/2018/10/05/081665ec9eb65fdce8a69473/statistik-tanaman-buah---buahan-dan-sayuran-tahunan-indonesia-2017 [17 March 2020].
  3. Barnes, I., Fourie, A., Wingfield, M. J., Harrington, T. C., Mc-New, D. L., Sugiyama, L. S., Luiz, B. C., Heller, W. P. and Keith, L. M. 2018. New Ceratocystis species associated with rapid death of Metrosideros polymorpha in Hawai'i. Persoonia 40:154-181. https://doi.org/10.3767/persoonia.2018.40.07
  4. Brawner, J., Japarudin, Y., Lapammu, M., Rauf, R., Boden, D. and Wingfield, M. J. 2015. Evaluating the inheritance of Ceratocystis acaciivora symptom expression in a diverse Acacia mangium breeding population. South. For. 77:83-90. https://doi.org/10.2989/20702620.2015.1007412
  5. Brito, R. A. S., Cavalcante, G. P., Borel, F. C. and Maffia, L. A. 2019. Detection and isolation of Ceratocystis fimbriata in mango trees on semi-selective medium. Eur. J. Plant Pathol. 155:667-669. https://doi.org/10.1007/s10658-019-01785-0
  6. Chen, S., Van Wyk, M., Roux, J., Wingfield, M. J., Xie, Y. and Zhou, X. 2013. Taxonomy and pathogenicity of Ceratocystis species on Eucalyptus trees in South China, including C. chinaeucensis sp. nov. Fungal Divers. 58:267-279. https://doi.org/10.1007/s13225-012-0214-5
  7. Engelbrecht, C. J. B. and Harrington, T. C. 2005. Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato, cacao and sycamore. Mycologia 97:57-69. https://doi.org/10.1080/15572536.2006.11832839
  8. Ferreira, M. A., Harrington, T. C., Piveta, G. and Alfenas, A. C. 2017. Genetic variability suggests that three populations of Ceratocystis fimbriata are responsible for the Ceratocystis wilt epidemic on kiwifruit in Brazil. Trop. Plant Pathol. 42:86-95. https://doi.org/10.1007/s40858-017-0131-y
  9. Fourie, A., Wingfield, M. J., Wingfield, B. D. and Barnes, I. 2015. Molecular markers delimit cryptic species in Ceratocystis sensu stricto. Mycol. Prog. 14:1020. https://doi.org/10.1007/s11557-014-1020-0
  10. Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  11. Hardie, M., Akhmad, N., Mohammed, C., Mendham, D., Corkrey, R., Gafur, A. and Siregar, S. 2018. Role of site in the mortality and production of Acacia mangium plantations in Indonesia. South. For. 80:37-50. https://doi.org/10.2989/20702620.2016.1274857
  12. Harrington, T. C., Huang, Q., Ferreira, M. A. and Alfenas, A. C. 2015. Genetic analyses trace the Yunnan, China population of Ceratocystis fimbriata on pomegranate and taro to populations on Eucalyptus in Brazil. Plant Dis. 99:106-111. https://doi.org/10.1094/PDIS-01-14-0056-RE
  13. Harrington, T. C., Kazmi, M. R., Al-Sadi, A. M. and Ismail, S. I. 2014. Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto. Mycologia 106:224-242. https://doi.org/10.3852/13-189
  14. Harrington, T. C., Thorpe, D. J. and Alfenas, A. C. 2011. Genetic variation and variation in aggressiveness to native and exotic hosts among Brazilian populations of Ceratocystis fimbriata. Phytopathology 101:555-566. https://doi.org/10.1094/PHYTO-08-10-0228
  15. Jimu, L., Wingfield, M. J., Mwenje, E. and Roux, J. 2015. Diseases on Eucalyptus species in Zimbabwean plantations and woodlots. South. For. 77: 221-230. https://doi.org/10.2989/20702620.2014.1001682
  16. Katoh, K., Rozewicki, J. and Yamada, K. D. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinform. 20:1160-1166. https://doi.org/10.1093/bib/bbx108
  17. Keith, L. M., Matsumoto, T. K. and McQuate, G. T. 2013. First report of Dolabra nepheliae associated with corky bark disease of langsat in Hawaii. Plant Dis. 97:990.
  18. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35:1547-1549. https://doi.org/10.1093/molbev/msy096
  19. Li, Q., Harrington, T. C., McNew, D., Li, J., Huang, Q., Somasekhara, Y. M. and Alfenas, A. C. 2016. Genetic bottlenecks for two populations of Ceratocystis fimbriata on sweet potato and pomegranate in China. Plant Dis. 100:2266-2274. https://doi.org/10.1094/PDIS-03-16-0409-RE
  20. Luchi, N., Ghelardini, L., Belbahri, L., Quartier, M. and Santini, A. 2013. Rapid detection of Ceratocystis platani inoculum by quantitative real-time PCR assay. Appl. Environ. Microbiol. 79:5394-5404. https://doi.org/10.1128/AEM.01484-13
  21. Moller, W. J. and DeVay, J. E. 1968. Carrot as a species-selective isolation medium for Ceratocystis fimbriata. Phytopathology 58:123-124.
  22. Naidoo, K., Steenkamp, E. T., Coetzee, M. P., Wingfield, M. J. and Wingfield, B. D. 2013. Concerted evolution in the ribosomal RNA cistron. PLoS ONE 8:e59355. https://doi.org/10.1371/journal.pone.0059355
  23. Nasution, A., Glen, M., Beadle, C. and Mohammed, C. 2019. Ceratocystis wilt and canker: a disease that compromises the growing of commercial Acacia-based plantations in the tropics. Aust. For. 82:80-93. https://doi.org/10.1080/00049158.2019.1595347
  24. Oliveira, L. S. S., Harrington, T. C., Ferreira, M. A., Damacena, M. B., Al-Sadi, A. M., Al-Mahmooli, I. H. S. and Alfenas, A. C. 2015. Species or genotypes? Reassessment of four recently described species of the Ceratocystis wilt pathogen, Ceratocystis fimbriata, on Mangifera indica. Phytopathology 105:1229-1244. https://doi.org/10.1094/PHYTO-03-15-0065-R
  25. Putranto, R.-A., Herlinawati, E., Rio, M., Leclercq, J., Piyatrakul, P., Gohet, E., Sanier, C., Oktavia, F., Pirrello, J., Kuswanhadi and Montoro, P. 2015. Involvement of ethylene in the latex metabolism and tapping panel dryness of Hevea brasiliensis. Int. J. Mol. Sci. 16:17885-17908. https://doi.org/10.3390/ijms160817885
  26. Roux, J. and Wingfield, M. J. 2009. Ceratocystis species: emerging pathogens of non-native plantation Eucalyptus and Acacia species. South. For. 71:115-120. https://doi.org/10.2989/SF.2009.71.2.5.820
  27. Suwandi, S., Junita, A., Suparman, S., Umayah, A., Hamidson, H., Muslim, A. and Irsan, C. 2018. Curative activity of watery fermented compost extract as a bark treatment against tapping panel dryness. Open Agric. J. 12:74-83. https://doi.org/10.2174/1874331501812010074
  28. Swofford, D. L. 2002. PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Associates, Sunderland, MA, USA.
  29. Tarigan, M., Roux, J., van Wyk, M., Tjahjono, B. and Wingfield, M. J. 2011. A new wilt and die-back disease of Acacia mangium associated with Ceratocystis manginecans and C. acaciivora sp. nov. in Indonesia S. Afr. J. Bot. 77:292-304. https://doi.org/10.1016/j.sajb.2010.08.006
  30. Tayler, V. A. and Stephens, J. 1929. Native rubber in the Dutch East Indies: report to the Rubber Growers' Association. Rubber Growers Association, London, UK. 48 pp.
  31. Techavuthiporn, C. 2018. Langsat - Lansium domesticum. In: Exotic fruits reference guide, eds. by S. Rodrigues, E. de Oliveira Silva and E. S. de Brito, pp. 279-283. Academic Press, New York, USA.
  32. Trang, T. T., Eyles, A., Davies, N., Glen, M., Ratkowsky, D. and Mohammed, C. 2018. Screening for host responses in Acacia to a canker and wilt pathogen, Ceratocystis manginecans. For. Pathol. 48:e12390. https://doi.org/10.1111/efp.12390
  33. van Wyk, M., Al Adawi, A. O., Khan, I. A., Deadman, M. L., Al Jahwari, A. A., Wingfield, B. D., Ploetz, R. and Wingfield, M. J. 2007. Ceratocystis manginecans sp. nov., causal agent of a destructive mango wilt disease in Oman and Pakistan. Fungal Divers. 27:213-230.
  34. Van Wyk, M., Al-Adawi, A. O., Wingfield, B. D., Al-Subhi, A. M., Deadman, M. L. and Wingfield, M. J. 2005. DNA based characterization of Ceratocystis fimbriata isolates associated with mango decline in Oman. Australas. Plant Pathol. 34:587-590. https://doi.org/10.1071/AP05080
  35. Van Wyk, M., Wingfield, B. D., Marin, M. and Wingfield, M. J. 2010. New Ceratocystis species infecting coffee, cacao, citrus and native trees in Colombia. Fungal Divers. 40:103-117. https://doi.org/10.1007/s13225-009-0005-9
  36. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  37. Whitman, W. F. 1980. Growing and fruiting the langsat in Florida. Proc. Fla. State Hortic. Soc. 93:136-140.
  38. Xu, K. C., Zhang, R. Q., Li, J., Bai, Y. H., Yang, X. D., Sun, Y. X. and Huang, Q. 2019. Camellia sinensis, a new host plant of Ceratocystis fimbriata from China. Plant Dis. 103:2670.
  39. Yaacob, O. and Bamroongrugsa, N. 1991. Lansium domesticum Correa. In: Plant resources of South-East Asia. No. 2. Edible fruits and nuts, eds. by E. Westphal, P. C. M. Jansen, E. W. M. Verheij and R. E. Coronel, pp. 186-190. Pudoc, Wageningen, The Netherlands.