• Title/Summary/Keyword: Bar development

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Optimization of a microarray for fission yeast

  • Kim, Dong-Uk;Lee, Minho;Han, Sangjo;Nam, Miyoung;Lee, Sol;Lee, Jaewoong;Woo, Jihye;Kim, Dongsup;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • v.17 no.3
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    • pp.28.1-28.9
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    • 2019
  • Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

A Study on the Basic Development Length of GFRP Rebar With Ribs (이형 GFRP 보강근의 기본정착길이에 대한 연구)

  • Moon, Do Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.5A
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    • pp.485-493
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    • 2010
  • GFRP rebar with ribs resemble those of deformed steel rebar was developed in 2005. It was reported that ribs of the GFRP rebar were sheared off due to the lower shear strength of polymer. In this study, the basic development length of the GFRP rebar was investigated through pull-out tests, models specified in ACI440.1R-03 and -06, and empirical model derived by Cosenza et al. (2002). As a results of pull-out tests, the critical embeddment length, which is defined as the length when failure mode is changed from pull-out to bar fracture, was 20 times of bar diameter for GFRP rebar and was 15 times for steel rebar. It is believed that the basic development of the GFRP rebar is 21 times of bar diameter, which is determined from the application of average bond strength into the model equation specified in ACI440.1R-03. Compared to the model equation in ACI440.1R-06, that in ACI440.1R-03 is recommendable for design purpose. The Cosenza et al.'s model underestimates the basic development length of the GFRP rebar.

A Study of The Medical Classics in the '$\bar{A}yurveda$' (아유르베다'($\bar{A}yurveda$) 의경(醫經)에 관한 연구)

  • Kim, Kj-Wook;Park, Hyun-Kuk;Seo, Ji-Young
    • The Journal of Dong Guk Oriental Medicine
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    • v.10
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    • pp.119-145
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    • 2008
  • Through a simple study of the medical classics in the '$\bar{A}yurveda$', we have summarized them as follows. 1) Traditional Indian medicine started in the Ganges river area at about 1500 B. C. E. and traces of medical science can be found in the "Rigveda" and "Atharvaveda". 2) The "Charaka(閣羅迦集)" and "$Su\acute{s}hruta$(妙聞集)", ancient texts from India, are not the work of one person, but the result of the work and errors of different doctors and philosophers. Due to the lack of historical records, the time of Charaka(閣羅迦) or $Su\acute{s}hruta$(妙聞)s' lives are not exactly known. So the completion of the "Charaka" is estimated at 1st$\sim$2nd century C. E. in northwestern India, and the "$Su\acute{s}hruta$" is estimated to have been completed in 3rd$\sim$4th century C. E. in central India. Also, the "Charaka" contains details on internal medicine, while the "$Su\acute{s}hruta$" contains more details on surgery by comparison. 3) '$V\bar{a}gbhata$', one of the revered Vriddha Trayi(triad of the ancients, 三醫聖) of the '$\bar{A}yurveda$', lived and worked in about the 7th century and wrote the "$Ast\bar{a}nga$ $Ast\bar{a}nga$ hrdaya $samhit\bar{a}$ $samhit\bar{a}$(八支集) and "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$(八心集)", where he tried to compromise and unify the "Charaka" and "$Su\acute{s}hruta$". The "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$" was translated into Tibetan and Arabic at about the 8th$\sim$9th century, and if we generalize the medicinal plants recorded in each the "Charaka", "$Su\acute{s}hruta$" and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", there are 240, 370, 240 types each. 4) The 'Madhava' focused on one of the subjects of Indian medicine, '$Nid\bar{a}na$' ie meaning "the cause of diseases(病因論)", and in one of the copies found by Bower in 4th century C. E. we can see that it uses prescriptions from the "BuHaLaJi(布唅拉集)", "Charaka", "$Su\acute{s}hruta$". 5) According to the "Charaka", there were 8 branches of ancient medicine in India : treatment of the body(kayacikitsa), special surgery(salakya), removal of alien substances(salyapahartka), treatment of poison or mis-combined medicines(visagaravairodhikaprasamana), the study of ghosts(bhutavidya), pediatrics(kaumarabhrtya), perennial youth and long life(rasayana), and the strengthening of the essence of the body(vajikarana). 6) The '$\bar{A}yurveda$', which originated from ancient experience, was recorded in Sanskrit, which was a theorization of knowledge, and also was written in verses to make memorizing easy, and made medicine the exclusive possession of the Brahmin. The first annotations were 1060 for the "Charaka", 1200 for the "$Su\acute{s}hruta$", 1150 for the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and 1100 for the "$Nid\bar{a}na$". The use of various mineral medicines in the "Charaka" or the use of mercury as internal medicine in the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and the palpation of the pulse for diagnosing in the '$\bar{A}yurveda$' and 'XiZhang(西藏)' medicine are similar to TCM's pulse diagnostics. The coexistence with Arabian 'Unani' medicine, compromise with western medicine and the reactionism trend restored the '$\bar{A}yurveda$' today. 7) The "Charaka" is a book inclined to internal medicine that investigates the origin of human disease which used the dualism of the 'Samkhya', the natural philosophy of the 'Vaisesika' and the logic of the 'Nyaya' in medical theories, and its structure has 16 syllables per line, 2 lines per poem and is recorded in poetry and prose. Also, the "Charaka" can be summarized into the introduction, cause, judgement, body, sensory organs, treatment, pharmaceuticals, and end, and can be seen as a work that strongly reflects the moral code of Brahmin and Aryans. 8) In extracting bloody pus, the "Charaka" introduces a 'sharp tool' bloodletting treatment, while the "$Su\acute{s}hruta$" introduces many surgical methods such as the use of gourd dippers, horns, sucking the blood with leeches. Also the "$Su\acute{s}hruta$" has 19 chapters specializing in ophthalmology, and shows 76 types of eye diseases and their treatments. 9) Since anatomy did not develop in Indian medicine, the inner structure of the human body was not well known. The only exception is 'GuXiangXue(骨相學)' which developed from 'Atharvaveda' times and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$". In the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$"'s 'ShenTiLun(身體論)' there is a thorough listing of the development of a child from pregnancy to birth. The '$\bar{A}yurveda$' is not just an ancient traditional medical system but is being called alternative medicine in the west because of its ability to supplement western medicine and, as its effects are being proved scientifically it is gaining attention worldwide. We would like to say that what we have researched is just a small fragment and a limited view, and would like to correct and supplement any insufficient parts through more research of new records.

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Development of Online Model for Mean Effective Strain, Roll Force and Area Reduction in Bar Rolling with Three Rolls (콕스밀에서 평균변형율, 압하력, 단면감소율에 대한 수식모델개발)

  • Je S. H.;Hwang S. M.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2001.10a
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    • pp.162-165
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    • 2001
  • In industrial practice, caliber design in shape rolling depends on the designer's experience, which in general is obtained through costly trial-and error process. on-line model which is relations of mean effective strain, roll force and area reduction is derived from finite element process simultion in bar rolling with three rolls.

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A Development of Single Action Press Robot (프레스 단동로봇의 개발)

  • 허성창;황병복
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1997.03a
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    • pp.261-264
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    • 1997
  • A single action press robot, which consists of a driving unit, rotator, up-down feed base and feed bar, is developed and applied for the press automation. The driving unit is made up with a face cam and blade cam, which have a phase angle. The feeding system consists of a double speed-up apparatus and linear motion guides, and has a fast motion characteristics. A horizontal feeding speed of the feed bar is increased twice by the double speed-up apparatus. The driving mechanism could be simplified due to the speed-up of the feeding unit.

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Effects on the Development of Plutella xylostella and Spodoptera litura after Feeding on Transgenic Cabbage Expressing Potato Proteinase Inhibitor II and Bar Genes

  • Lee, Yeon-Hee;Lee, Sang-Guei;Park, Beom-Seok;Lee, Young-Su;Jin, Yong-Moon;Kim, Ho-il;Suh, Seok-Cheol
    • Journal of Plant Biotechnology
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    • v.6 no.3
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    • pp.145-150
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    • 2004
  • Cabbage plants were transformed with the potato proteinase inhibitor II (PINII) gene, bar gene, and hpt gene using Agrobacterium. The expression of the PINII gene was driven by its own promoter which was wound-inducible. Ten transgenic plants were obtained from medium containing hygromycin as a selection antibiotic. The integration and expression of PINII and bar genes were confirmed by Southern and Northern hybridization. Growth and development of diamondback moths (Plutella xylostella) and tobacco cutworm (Spodoptera litura) larvae were examined on $T_1$ plants. The weight of the larvae and pupae of these two insects grown on transgenic plants was not different compared to those grown on wild type plants. However, the pupation and emergence rate of diamondback moths and tobacco cutworms fed on some transgenic plants was lower than on wild type plants. These results suggest that the PINII transgene under the control of a wound-induced promoter may be used for control of insects in transgenic cabbage through reduction of insect progeny number.

Development of composite torsion shaft for the aircraft structure under multiple load condition (복합하중을 받는 복합소재 중공 토크바 설계)

  • Jeong, Jong-Jae;Kim, Seung-Chul;Kim, Jeong
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.6
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    • pp.484-491
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    • 2016
  • The purpose of this development is weight reduction of hollow type steel torque bar by changing the material from steel to composite. Structure analysis is executed by the finite element model generated by the structural load condition and geometric structure requirement. According to this analysis result, optimized ply sequence and wall thickness are defined. To simulate analysis result, torsion test for composite torque bar was performed. Throughout the test result, the stiffness and strength requirement of composite torque bar was verified.

A Study of Laboratory Measurement of EO GRD Resolution for Airborne EO/IR Sensor (항공용 EO/IR 센서의 EO GRD 분해능 실험실 측정 연구)

  • Huh, Joon;Kim, Chang-Woo;Kim, Sungsoo;Kim, Byoung-Wan
    • Journal of the Korea Institute of Military Science and Technology
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    • v.17 no.6
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    • pp.793-799
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    • 2014
  • EO GRD(Ground Resolved Distance) resolution of airborne EO/IR(Electro-Optical/Infrared) sensor is a critical factor in test and evaluation for EO sensor performance. We propose the laboratory measurement set-up for EO GRD by constructing optical collimator which includes integrated sphere, blackbody, equivalent 3-bar target and 6 DOF motion simulator. GRD is measured in the photographic imagery of bar targets by 3 different distances for 3 EO/IR sensors and the measured results were analyzed statistically. We found that at least 7 sheets of imagery are needed in order to obtain meaningful EO GRD. The result of statistical analysis shows that the distribution of the measured GRD is nearly symmetric about the average GRD, and the better imagery ratio above the average GRD is about 40~70%. Also from the best GRD analysis, it is estimated that the design goal for EO GRD should be 30% superior to the required GRD.

A Study on the Mechanical Properties of Gas Pressure Welded Splices of Deformed Reinforcing Bar (가스압접 이형철근의 기계적 강도 특성 연구)

  • Jeon, Juntai
    • Journal of the Society of Disaster Information
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    • v.11 no.4
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    • pp.520-526
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    • 2015
  • Reinforcing bar splices are inevitable in reinforced concrete structure. In these days, there are three main types of splices used in reinforced concrete construction site - lapped splice, mechanical splice and welded splice. Low cost, practicality in construction site, less time consuming and high performance make gas pressure welding become a favorable splice method. However, reinforcing bar splice experiences thermal loading history during the welding procedure. This may lead to the presence of residual stress in the vicinity of the splice which affects the fatigue life of the reinforcing bar. Therefore, residual stress analysis and tensile test of the gas pressure welded splice are carried out in order to verify the load bearing capacity of the gas pressure welded splice. The reinforcing bar used in this work is SD400, which is manufactured in accordance with KS D 3504. The results show that the residual stresses in welded splice is relatively small, thus not affecting the performance of the reinforcing bar. Moreover, the strength of the gas pressure welded splice is high enough for the development of yielding in the bar. As such, the reinforcing bar with gas pressure welded splice has enough capacity to behave as continuous bar.