• 제목/요약/키워드: Bar development

검색결과 827건 처리시간 0.023초

Optimization of a microarray for fission yeast

  • Kim, Dong-Uk;Lee, Minho;Han, Sangjo;Nam, Miyoung;Lee, Sol;Lee, Jaewoong;Woo, Jihye;Kim, Dongsup;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • 제17권3호
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    • pp.28.1-28.9
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    • 2019
  • Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

이형 GFRP 보강근의 기본정착길이에 대한 연구 (A Study on the Basic Development Length of GFRP Rebar With Ribs)

  • 문도영
    • 대한토목학회논문집
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    • 제30권5A호
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    • pp.485-493
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    • 2010
  • 국내에서 개발된 이형 GFRP 보강근은 표면에 구축된 이형의 전단강도가 콘크리트의 전단강도 보다 상대적으로 작아 이형철근과 달리 이형 자체가 전단파괴되는 파괴모드를 보이는 것으로 확인된바 있다. 본 논문에서는 이형을 갖는 GFRP 보강근의 기본정착길이를 인발실험과 설계모델식과 해석적 엄밀식을 통해 고찰하였다. 실험결과, 동일조건하에서 파괴모드가 변화되는 임계정착길이가 이형철근은 직경의 15배, 이형 GFRP 보강근은 20배인 것으로 나타났다. 또한 실험결과를 ACI440.1R-03 설계모델식에 적용하여 분석한 결과, 충분한 횡구속이 수반된 경우 직경 9 mm의 이형 GFRP 보강근의 기본정착길이는 직경의 21배인 것으로 나타났다. 반면, ACI440.1R-06에 제시된 기본정착길이 모델은 실험결과에 비하여 너무 과대한 기본정착길이를 요구하는 것으로 나타났다. Cosenza 등(2002)의 모델은 실험결과에 비하여 더 적은 기본 정착길이를 요구하므로, 설계목적의 사용은 제한적인 것으로 판단되었다.

아유르베다'($\bar{A}yurveda$) 의경(醫經)에 관한 연구 (A Study of The Medical Classics in the '$\bar{A}yurveda$')

  • 김기욱;박현국;서지영
    • 동국한의학연구소논문집
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    • 제10권
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    • pp.119-145
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    • 2008
  • Through a simple study of the medical classics in the '$\bar{A}yurveda$', we have summarized them as follows. 1) Traditional Indian medicine started in the Ganges river area at about 1500 B. C. E. and traces of medical science can be found in the "Rigveda" and "Atharvaveda". 2) The "Charaka(閣羅迦集)" and "$Su\acute{s}hruta$(妙聞集)", ancient texts from India, are not the work of one person, but the result of the work and errors of different doctors and philosophers. Due to the lack of historical records, the time of Charaka(閣羅迦) or $Su\acute{s}hruta$(妙聞)s' lives are not exactly known. So the completion of the "Charaka" is estimated at 1st$\sim$2nd century C. E. in northwestern India, and the "$Su\acute{s}hruta$" is estimated to have been completed in 3rd$\sim$4th century C. E. in central India. Also, the "Charaka" contains details on internal medicine, while the "$Su\acute{s}hruta$" contains more details on surgery by comparison. 3) '$V\bar{a}gbhata$', one of the revered Vriddha Trayi(triad of the ancients, 三醫聖) of the '$\bar{A}yurveda$', lived and worked in about the 7th century and wrote the "$Ast\bar{a}nga$ $Ast\bar{a}nga$ hrdaya $samhit\bar{a}$ $samhit\bar{a}$(八支集) and "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$(八心集)", where he tried to compromise and unify the "Charaka" and "$Su\acute{s}hruta$". The "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$" was translated into Tibetan and Arabic at about the 8th$\sim$9th century, and if we generalize the medicinal plants recorded in each the "Charaka", "$Su\acute{s}hruta$" and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", there are 240, 370, 240 types each. 4) The 'Madhava' focused on one of the subjects of Indian medicine, '$Nid\bar{a}na$' ie meaning "the cause of diseases(病因論)", and in one of the copies found by Bower in 4th century C. E. we can see that it uses prescriptions from the "BuHaLaJi(布唅拉集)", "Charaka", "$Su\acute{s}hruta$". 5) According to the "Charaka", there were 8 branches of ancient medicine in India : treatment of the body(kayacikitsa), special surgery(salakya), removal of alien substances(salyapahartka), treatment of poison or mis-combined medicines(visagaravairodhikaprasamana), the study of ghosts(bhutavidya), pediatrics(kaumarabhrtya), perennial youth and long life(rasayana), and the strengthening of the essence of the body(vajikarana). 6) The '$\bar{A}yurveda$', which originated from ancient experience, was recorded in Sanskrit, which was a theorization of knowledge, and also was written in verses to make memorizing easy, and made medicine the exclusive possession of the Brahmin. The first annotations were 1060 for the "Charaka", 1200 for the "$Su\acute{s}hruta$", 1150 for the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and 1100 for the "$Nid\bar{a}na$". The use of various mineral medicines in the "Charaka" or the use of mercury as internal medicine in the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and the palpation of the pulse for diagnosing in the '$\bar{A}yurveda$' and 'XiZhang(西藏)' medicine are similar to TCM's pulse diagnostics. The coexistence with Arabian 'Unani' medicine, compromise with western medicine and the reactionism trend restored the '$\bar{A}yurveda$' today. 7) The "Charaka" is a book inclined to internal medicine that investigates the origin of human disease which used the dualism of the 'Samkhya', the natural philosophy of the 'Vaisesika' and the logic of the 'Nyaya' in medical theories, and its structure has 16 syllables per line, 2 lines per poem and is recorded in poetry and prose. Also, the "Charaka" can be summarized into the introduction, cause, judgement, body, sensory organs, treatment, pharmaceuticals, and end, and can be seen as a work that strongly reflects the moral code of Brahmin and Aryans. 8) In extracting bloody pus, the "Charaka" introduces a 'sharp tool' bloodletting treatment, while the "$Su\acute{s}hruta$" introduces many surgical methods such as the use of gourd dippers, horns, sucking the blood with leeches. Also the "$Su\acute{s}hruta$" has 19 chapters specializing in ophthalmology, and shows 76 types of eye diseases and their treatments. 9) Since anatomy did not develop in Indian medicine, the inner structure of the human body was not well known. The only exception is 'GuXiangXue(骨相學)' which developed from 'Atharvaveda' times and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$". In the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$"'s 'ShenTiLun(身體論)' there is a thorough listing of the development of a child from pregnancy to birth. The '$\bar{A}yurveda$' is not just an ancient traditional medical system but is being called alternative medicine in the west because of its ability to supplement western medicine and, as its effects are being proved scientifically it is gaining attention worldwide. We would like to say that what we have researched is just a small fragment and a limited view, and would like to correct and supplement any insufficient parts through more research of new records.

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콕스밀에서 평균변형율, 압하력, 단면감소율에 대한 수식모델개발 (Development of Online Model for Mean Effective Strain, Roll Force and Area Reduction in Bar Rolling with Three Rolls)

  • 제상현;황상무
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2001년도 추계학술대회 논문집
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    • pp.162-165
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    • 2001
  • In industrial practice, caliber design in shape rolling depends on the designer's experience, which in general is obtained through costly trial-and error process. on-line model which is relations of mean effective strain, roll force and area reduction is derived from finite element process simultion in bar rolling with three rolls.

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프레스 단동로봇의 개발 (A Development of Single Action Press Robot)

  • 허성창;황병복
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 1997년도 춘계학술대회논문집
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    • pp.261-264
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    • 1997
  • A single action press robot, which consists of a driving unit, rotator, up-down feed base and feed bar, is developed and applied for the press automation. The driving unit is made up with a face cam and blade cam, which have a phase angle. The feeding system consists of a double speed-up apparatus and linear motion guides, and has a fast motion characteristics. A horizontal feeding speed of the feed bar is increased twice by the double speed-up apparatus. The driving mechanism could be simplified due to the speed-up of the feeding unit.

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Effects on the Development of Plutella xylostella and Spodoptera litura after Feeding on Transgenic Cabbage Expressing Potato Proteinase Inhibitor II and Bar Genes

  • Lee, Yeon-Hee;Lee, Sang-Guei;Park, Beom-Seok;Lee, Young-Su;Jin, Yong-Moon;Kim, Ho-il;Suh, Seok-Cheol
    • Journal of Plant Biotechnology
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    • 제6권3호
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    • pp.145-150
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    • 2004
  • Cabbage plants were transformed with the potato proteinase inhibitor II (PINII) gene, bar gene, and hpt gene using Agrobacterium. The expression of the PINII gene was driven by its own promoter which was wound-inducible. Ten transgenic plants were obtained from medium containing hygromycin as a selection antibiotic. The integration and expression of PINII and bar genes were confirmed by Southern and Northern hybridization. Growth and development of diamondback moths (Plutella xylostella) and tobacco cutworm (Spodoptera litura) larvae were examined on $T_1$ plants. The weight of the larvae and pupae of these two insects grown on transgenic plants was not different compared to those grown on wild type plants. However, the pupation and emergence rate of diamondback moths and tobacco cutworms fed on some transgenic plants was lower than on wild type plants. These results suggest that the PINII transgene under the control of a wound-induced promoter may be used for control of insects in transgenic cabbage through reduction of insect progeny number.

복합하중을 받는 복합소재 중공 토크바 설계 (Development of composite torsion shaft for the aircraft structure under multiple load condition)

  • 정종재;김승철;김정
    • 한국항공우주학회지
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    • 제44권6호
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    • pp.484-491
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    • 2016
  • 본 연구의 목적은 항공기 메커니즘 구조물에 사용되는 금속 소재 토크바를 복합소재를 이용하여 무게절감이 가능한 구조물을 개발 하는것에 있다. 토크바에 주어진 하중조건 및 구조물의 설계 경계조건에 부합하는 유한요소모델을 이용하여 구조해석을 수행 하였다. 이를 바탕으로 최적화된 복합소재의 적층 조건 및 토크바의 두께 요건을 설정하였다. 설계요건에 맞게 제작된 시제품을 이용하여 복합소재 토크바의 비틀림 시험을 실시하였으며, 동구조 시험을 통해서 토크바의 강도 및 강성 요구조건을 만족한다는 것을 증명하였다.

항공용 EO/IR 센서의 EO GRD 분해능 실험실 측정 연구 (A Study of Laboratory Measurement of EO GRD Resolution for Airborne EO/IR Sensor)

  • 허준;김창우;김성수;김병완
    • 한국군사과학기술학회지
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    • 제17권6호
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    • pp.793-799
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    • 2014
  • EO GRD(Ground Resolved Distance) resolution of airborne EO/IR(Electro-Optical/Infrared) sensor is a critical factor in test and evaluation for EO sensor performance. We propose the laboratory measurement set-up for EO GRD by constructing optical collimator which includes integrated sphere, blackbody, equivalent 3-bar target and 6 DOF motion simulator. GRD is measured in the photographic imagery of bar targets by 3 different distances for 3 EO/IR sensors and the measured results were analyzed statistically. We found that at least 7 sheets of imagery are needed in order to obtain meaningful EO GRD. The result of statistical analysis shows that the distribution of the measured GRD is nearly symmetric about the average GRD, and the better imagery ratio above the average GRD is about 40~70%. Also from the best GRD analysis, it is estimated that the design goal for EO GRD should be 30% superior to the required GRD.

가스압접 이형철근의 기계적 강도 특성 연구 (A Study on the Mechanical Properties of Gas Pressure Welded Splices of Deformed Reinforcing Bar)

  • Jeon, Juntai
    • 한국재난정보학회 논문집
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    • 제11권4호
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    • pp.520-526
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    • 2015
  • 철근 콘크리트 구조에서 철근의 이음은 불가피하게 사용된다. 최근 들어 철근 콘크리트 구조물에 가장 많이 사용되고 있는 철근 이음에는 겹침 이음, 기계 이음, 그리고 용접 접합 등이 있다. 이중에서 저비용, 건설 현장에서의 실용성, 공사 기간 단축 및 고성능 등의 장점으로 인하여 가스 압접 이음의 효용성이 대두되고 있다. 그러나 가스 압접 이음 과정동안 철근이 열을 받게 되고 이는 접합부 주위에 잔류 응력으로 남아 철근의 피로수명에 영향을 미칠 수 있다. 그러므로 가스 압접 접합부의 명확한 잔류 응력 분석과 가스 압접 후 철근의 하중지지 능력을 확인하기 위한 인장 시험이 수행되어야 한다. 이 연구에서는 공용중인 철근(KS D3504 SD400)에 대하여 3차원 해석을 수행하여 연구 결과 잔류 응력은 상대적으로 작기 때문에 철근의 피로 수명에 영향을 미치지 않으며 인장 실험 결과에서도 가스 압접된 철근의 항복강도가 기준항복강도보다 높게 측정되어 하중 저항 능력도 가스 압접 이음부가 연속된 철근으로서의 거동에 충분히 그 성능을 발휘하는 것으로 나타났다.