• Title/Summary/Keyword: Baek-Kyung

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Overproduction of Recombinant Human VEGF (Vascular Endothelial Growth Factor) in Chinese Hamster Ovary Cells

  • Lee, Seong-Baek;Park, Jeong-Soo;Lee, Seung-Hee;Park, Jun-Ho;Yu, Sung-Ryul;Kim, Hee-Chan;Kim, Dong-Jun;Byun, Tae-Ho;Baek, Kwang-Hee;Ahn, Young-Joon;Yoon, Jae-Seung
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.183-187
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    • 2008
  • Vascular endothelial growth factors (VEGFs) are a family of proteins that mediate angiogenesis. $VEGF_{165}$ is a VEGF-A isoform and has been extensively studied owing to its potential use in therapeutic angiogenesis. This study established Chinese hamster ovary (CHO) cells overexpressing recombinant human $VEGF_{165}$ $(rhVEGF_{165})$ protein. The production rate of the established CHO cells was over 80mg/l of $rhVEGF_{165}$ protein from a 7-day batch culture process using a 7.5-l bioreactor with a 5-l working volume and serum-free medium. The $rhVEGF_{165}$ protein was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure that resulted in a 48% recovery rate. The purified $rhVEGF_{165}$ protein was a glycosylated homodimeric protein with a higher molecular weight (MW) than the protein expressed from insect cells, suggesting that the glycosylation of the $rhVEGF_{165}$ protein in CHO cells differed from that in insect cells. The purified $rhVEGF_{165}$ protein in this study was functionally active with a half-maximal effective concentration of 3.8ng/ml and specific activity of $2.5{\times}10^5U/mg$.

MAXILLARY SINUSITIS AS A COMPLICATION OF ORAL BISPHOSPHONATE RELATED OSTEONECROSIS OF THE JAW: A CASE REPORT (경구용 비스포스포네이트 관련 악골괴사의 합병증로 발생한 상악동염; 증례보고)

  • Kim, Yeong-Ran;Kwon, Yong-Dae;Lee, Baek-Soo;Choi, Byung-Joon;Walter, Christian;Al-Nawas, Bilal
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.35 no.1
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    • pp.39-40
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    • 2009
  • Maxillary sinusitis is an infectious disease which can arise from odontogenic etiology and a maxillary osteomyelitis can spread into the sinus and consequently develop maxillary sinusitis. In this case report, a mid eighty's lady was diagnosed as BRONJ with maxillary sinusitis as a complication. The patient was managed successfully in collaboration with a endocrinologist. Through serial follow-up of serum CTX, we could decide the timing of surgical intervention.

Phytosterols from the Rice (Oryza sativa) Bran

  • Jung, Ye-Jin;Park, Ji-Hae;Shrestha, Sabina;Song, Myoung-Chong;Cho, Suengmok;Lee, Chang-Ho;Han, Daeseok;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.175-178
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    • 2014
  • Three phytosterols of rare occurrence, schleicheol 2 (1), $7{\beta}$-hydroxysitosterol (2), and $7{\alpha}$-hydroxysitosterol (3), were isolated from the n-hexane fraction of rice (Oryza sativa) bran, for the first time. Some nuclear magnetic resonance (NMR) assignments in the literatures are inaccurate. This study employed two-dimensional NMR experiments to identify exact peak assignments.

Development of the platform independent music streaming web services based HTML5 (플렛폼 독립적인 HTML5 기반의 음원 스트리밍 웹서비스 개발)

  • Choi, Jae-Sung;Kwon, Hang-Geul;Park, Si-Hong;Baek, Tae-San
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.955-957
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    • 2014
  • HTML5에 들어서면서 웹서비스 구현에 보편적으로 사용되는 신기술들이 속속들이 나오고 있다. 브라우저 자체적인 그래픽 구현을 위한 Canvas/SVG, 멀티미디어와 관련된 audio, video, webaudio API 등이 추가되었고, 이로 인해 HTML5는 웹 환경에 더 빠르고 정교하며 편리한 인터페이스를 제공해 주었다. 이 중 아직도 표준화가 진행 중이거나 많은 브라우저에서 지원하지 않아 섣불리 사용 할 수 없는 기술들 또한 상당 수 존재한다. 본 논문에서 HTML5기술들을 활용하여 다양한 플랫폼에서 공통적으로 동작되는 플랫폼 독립적인 음원 스트리밍 웹서비스를 개발한다.

The Constituents of the Aerial Part of Gastrodia elata Blume

  • Liu, Xiang Qian;Baek, Wan-Sook;Ahn, Duk-Kyun;Choi, Ho-Young;Yook, Chang-Soo
    • Natural Product Sciences
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    • v.8 no.4
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    • pp.137-140
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    • 2002
  • From the MeOH extract of the aerial part of Gastrodia elata Blume (GEB) (Family: Orchidaceae), eight compounds have been isolated on repeated column chromatography, and their structures were elucidated as dotriacontanoic acid (1), beta-sitosterol (2), 4-hydroxybenzaldehyde (3), docosanoic acid oxiranylmethyl ester (4), hentriacotanoic acid (5), octadecanoic acid (6), benzoic acid (7) and gastrodin (8) on the basis of their spectral evidences including EI-Mass and 2D-NMR spectrum. All of them were obtained from the aerial part of Gastrodia elata Blume for the first time, in which compound 4 is a novelty to our best knowledge. It is also known that a phenolic glucoside, gastrodin is a major constituent just like Gastrodia rhizome.

Protective Roles of Mushrooms in Experimental Colon Carcinogenesis

  • Kim, Dong-Hyun;Jang, Il-Sung;Park, Jong-Baek;Lee, Seung-Won
    • Archives of Pharmacal Research
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    • v.18 no.2
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    • pp.79-83
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    • 1995
  • There is epidemilogical evidence that the population with high fecal ${\beta}-glucuronidase$activity has greater risk of colon cancer than the population with low facal ${\beta}-glucuronidase$. This relationship was investigated by using the mouse-dimethylhydrazine colon carcinogenesis model and the fraction of Glucidum which is a .${\beta}-glucuronidase$inhibitor. Mice with low facal ${\beta}-glucuronidase$activity induced by consumption of the ether fraction of G lucidum had significantly fewer aberrant crypts(AC) after injections of 1, 2-dimethylhydeazine (DMH) than mice treated with DMH alone. The result supports the hypothesis that the inhibitor such as the ether fraction of G lucidum can protect an animal against the induction of colon cancer.

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