• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.799-805
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• 2016

Methicillin-resistant Staphylococcus aureus (MRSA), the major causative agent of nosocomial infection, has also been reported from non-human sources. A sequence type (ST) 541 MRSA isolate designated K12PJN53 was isolated from a healthy pig in 2012. The genome of K12PJN53 consists of 44 contiguous sequences (contigs), totalling 2,880,108 bases with 32.88% GC content. Among the annotated contigs, 14, 17, and 18 contained genes related to antimicrobial resistance, adherence, and toxin genes, respectively. The genomic distance of strain K12PJN53 was close to the ST398 strains. This is the first report of the draft genome sequence of a novel livestock-associated MRSA ST541 strain.

• 한국작물학회지
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• 제65권4호
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• pp.314-326
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• 2020

본 연구는 유전자의 확대와 집적을 통해 벼 흰잎마름병균 K3a에 대응하는 저항성 계통을 육성하였고 육성계통에 대한 육종과정, 병 저항성반응을 분석하여 저항성 계통의 기초자료와 재배 효과를 제공하여 우수한 벼 흰잎마름병 저항성 품종 개발에 활용하고자 실험을 수행하였다. 벼 흰잎마름병 저항성 Xa3 유전자를 가지고 있는 중만생 자포니카 품종 황금누리를 반복친으로, Xa21 유전자를 가진 중만생 인디카 근동질유전자계통 IRBB21을 수여친으로 인공교배 후 3번 여교배하여 ABLs21을 얻었다. 생물검정과 분자표지검정을 활용하여 저항성 유전자 Xa3, Xa21이 집적을 확인하였다. ABLs21이 보유한 저항성 유전자는 분자표지 9643.T4 (Xa3), U1/I1 (Xa21)로 PCR 한 결과 모두 증폭되어 저항성 유전자를 가지는 것으로 확인되었다. ABLs21과 모부본의 벼 흰잎마름병 레이스에 대한 저항성 반응은 황금누리, IRBB3가 K1, K2, K3 레이스에 저항성 반응을 보였지만 K3a, K4, K5에는 감수성 반응을 나타냈다. IRBB21은 K1에 감수성 반응이었고 K2~K5에는 저항성 반응이었다. K3a 레이스 균주 접종 시 유묘단계에서 황금누리, IRBB21, ABL21-1, 분얼단계에서 황금누리, IRBB21, 성체단계에서 황금누리가 감수성 반응이었다. ABL21-1은 분얼단계에서 중도저항성을, 성체단계에서 저항성 반응을 나타냈다. K3a 레이스 18개 균주 접종 결과 ABL21-1은 각각의 수여친보다 병반길이와 표준편차가 작아 안정적인 저항성을 보여주었다. 18개 균주 각각의 반복간 병반길이의 유의차는 없어 균주의 병원성은 안정적이었으며 군집분석 결과 HB4032 균주의 병원력이 가장 큰 것으로 나타났다. ABLs21의 분자표지 다형성은 63.2%이며 평균 86.1 cM의 염색체단편이 이입되었다. ABLs21의 Xa21 유전자 부위로 추정되는 곳에 수여친의 염색체단편 이입이 일어났다.

• 한국염색가공학회지
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• 제26권4호
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• pp.322-330
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• 2014

The phospholipid-based MPCE(2-Methacryloxyethyl Phosphoryl Chlorine) copolymer was mixed with wet polyurethane for coating of nylon fabrics. The substitution rate of water in coagulation bath with DMF was changed under control of the size of formed hydrophilic microporous cell enable to manufacture excellent breathable, anti-bacterial and moisture control fabrics. Biocompatible property, vapor permeability, hydrostatic pressure, moisture management and anti-bacterial property were investigated for treated nylon fabrics. In result, increased moisture transmission rates, decreased water resistance and outstanding moisture control property could be confirmed by enhanced hydrophilicity of wet-coated nylon fabric with MPCE copolymer.

• Journal of Microbiology
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• 제44권4호
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• pp.453-456
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• 2006

16 chicken isolates and four clinical isolates of VanB-vanA incongruent vancomycinresistant Enterococcus faecium strains without vanS were isolated in 1999. Pulsed-field gel electrophoresis revealed only a peripheral relationship between the chicken isolates and clinical isolates, but suggested clonal spread in the chicken isolates.

• Applied Biological Chemistry
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• 제19권2호
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• pp.75-81
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• 1976

세균포자의 안정성과 관련하여 DPA-Ca-spore complex의 해리에 영향을 주는 여러 요인중 15%까지의 NaCl 농도에 의해서 이루어 지는 높은 이온 강도의 효과에 대하여 실험하고 다음과 같이 결론을 얻었다. 1) NaCl에 의한 포자 현탁액중의 이온강도의 변화는 포자로 부터 Ca-DPA의 해리를 촉진시켜 주며 이런 현상은 포자의 열 저항성을 감소시키고 따라서 보관중 포자의 안정성이 상당히 저하 되었다. 2) 세포질의 membrane은 포자내에서 Ca-DPA의 안정성을 유지하는데 permeability barrier로써 중요한 역할을 한다.

• Pediatric Infection and Vaccine
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• 제16권1호
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• pp.6-12
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• 2009

There are relatively few novel antimicrobial agents despite the dramatic increase in antimicrobial resistance and multiple drug resistance of clinical isolates worldwide. Vancomycin is still the most widely used antibiotic for treating resistant Gram-positive coccal infections in children, especially for methicillin-resistant Staphylococcus aureus. For children with Gram-positive coccal infections where vancomycin is not effective or older therapeutic agents cannot be tolerated, linezolid, quinupristin-dalfopristin or daptomycin may be useful in the appropriate clinical setting. For Gram-negative bacterial infections, new carbapenems await clinical application. Tebipenem pivoxil is a novel oral carbapenem undergoing clinical trials for acute otitis media in pediatric patients. Antiviral drug development is now progressing at the pace of antibiotic development 30 years ago. Newer antiviral agents used for the treatment of herpes viruses and hepatitis C virus infections in children are included in this review.

• The Plant Pathology Journal
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• 제37권5호
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• pp.489-493
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• 2021

Bacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among P. syringe pv. actinidiae strains were determined by examining the presence of strA-strB or aadA, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the rpsL gene. All four streptomycin-resistant strains of P. syringe pv. actinidiae investigated in this study contained strA-strB as a resistant determinant. The presence of the aadA gene and a mutation in codon 43 of the rpsL gene was not identified.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1989년도 추계 학술대회지
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• pp.28-29
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• 1989

• 한국축산식품학회지
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• 제38권1호
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• pp.203-208
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• 2018

The objective of this study was to investigate the pathogenicity and antimicrobial resistance of foodborne pathogens isolated from farmstead cheeses. Twenty-seven isolates, including 18 Bacillus cereus, two Escherichia coli, and seven Staphylococcus aureus, were subjected to polymerase chain reaction (PCR) to detect virulence genes and toxin genes, and the antibiotic resistances of the isolates were determined. All E. coli isolates were determined by PCR to be non-pathogenic. Among the 18 B. cereus isolates, 17 isolates (94.4%) were diarrheal type, as indicated by the presence of nheA, entFM, hbIC, cytK and bceT genes, and one isolate (5.6%) was emetic type, based on the presence of the CER gene. Among the seven S. aureus isolates, three (42.9%) had the mecA gene, which is related to methicillin-resistance. Most B. cereus isolates (94.7%) showed antibiotic resistance to oxacillin and penicillin G, and some strains also showed resistance to ampicillin (26.3%), erythromycin (5.3%), tetracycline (10.5%), and vancomycin (5.3%). These results indicate that microbial food safety measures for farmstead cheese must be implemented in Korea because antibiotic resistant foodborne pathogens, with resistance even to vancomycin, harboring virulence genes were found to be present in the final products of farmstead cheese.

• 한국작물학회지
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• 제49권5호
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• pp.429-433
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• 2004

A single recessive gene, rxp, controls the bacterial leaf pustule (BLP) resistance in soybean and in our previous article, it has been mapped on linkage group (LG) D2 of molecular genetic map of soybean. A total of 130 recombinant inbred lines (RILs) from a cross between BLP-resistant SS2-2 and BLP-susceptible Jangyeobkong were used to identify molecular markers linked to rxp. Fifteen simple sequence repeat (SSR) markers on LG D2 were screened to construct a genetic map of rxp locus. Only four SSR markers, Satt135, Satt372, Satt448, and Satt486, showed parental polymorphisms. Using these markers, genetic scaffold map was constructed covering 26.2cM. Based on the single analysis of variance, Satt372 among these four SSR markers was the most significantly associated with the resistance to BLP. To develop new amplified fragment length polymorphism (AFLP) marker linked to the resistance gene, bulked segregant analysis (BSA) was employed. Resistance and susceptible bulks were made by pooling equal amount of genomic DNAs from ten of each in the segregating population. A total of 192 primer combinations were used to identify specific bands to the resistance, selecting three putative AFLP markers. These AFLP markers produced the fragment present in SS2-2 and the resistant bulk, and not in Jangyeobkong and the susceptible bulk. Linkage analysis revealed that McctEact97 $(P=0.0004,\;R^2=14.67\%)$ was more significant than Satt372, previously reported as the most closely linked marker.