• The Journal of Korean Physical Therapy
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• v.11 no.3
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• pp.81-87
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• 1999

The purpose of this study was to determine the effect of electrical stimulation on the number of MCs and percent of degranulated MCs in rat skin. Twelve male Sprague-Dawley rats were divided into two group; electrical stimulation group (n=6) and control group (n=6). Each animals hair on the back was removed. The electrical stimulation group received an positive rectangular pulsed electrical stimulation, while the control group was given the same treatment without electricity. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with toluidine blue-nuclear fast red and alcian blue-safranin O. respectively. The MCs were counted using a light microscope and computerized image analysis system and calculated as the density and the percent. A t-test showed a significantly higher density of MCs in the electrical stimulated rats than control rats(p<0.05), and the percent of degranulated MCs in the electrical stimulated rats was higher than in the control rats (p<0.05) in toluidine blue stained sections. The density of MCs was significantly higher in the electrical stimulated rats than the control rats in alcian blue-safranin O Stained sections (P<0.01). An analysis of variance showed that the densities of CTMCs was significantly lower than the densities of MMCs and mixed MCs in electrical stimulated rat in alcian blue-safranin O Stained sections (p<0.05). These results suggest that the electrical stimulation may have potential for increasing the number of MCs and lead to degranulate the MCs in rat skin.

• The Journal of Korean Physical Therapy
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• v.12 no.1
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• pp.1-7
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• 2000

The purpose of this study was to determine the effect of microamperage electrical stimulation on the number of argyrophilic nucleolar organizer region (AgNOR) in rat skin. Twenty four male Sprague-Dawley rats were divided into electrical stimulation and control group. Bach animals hair on the back was removed. The electrical stimulation group received an positive rectangular positive electrical stimulation with $500{\mu}A$, while the control group was given the same treatment without electricity. The rats were sacrificed at 4 and 7 day of stimulation, respectively. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with silver nitrate. The AgNOR were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the epidermal keratinocyte. In control skin, the mean AgNOR count of epidermal keratinocyte at 4 and 7 day were 1.67 and 1.72, whereas electrical stimulated rat had mean AgNOR counts of 2.0 and 2.14, respectively. A Student's t-test showed a significantly higher mean AgNOR number at 4 ana 7 day in the electrical stimulated rats than control rats (p<0.05). The microamperage electric current stimulation increased the epidermal AgMOR expression in incisional wound skin. These results suggest that the microamperage electrical stimulation may promote migration and proliferative activity of epidermal keratinocyte in surgical wound.

• Journal of Veterinary Clinics
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• v.28 no.3
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• pp.318-322
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• 2011

Three dogs with severe traumatic spinal cord injury (TSCI) due to falling wound were admitted to the Veterinary Medical Center, Chonbuk National University for evaluation of severe pelvic limbs paralysis without deep pain, normal defecation and urination. Based on physical examination, neurological assessment and computed tomogram (CT), the diagnosis was made as subluxation and compressed fracture. All the cases were surgically treated with dorsal laminectomy and a spondylosyndesis using pin and bone cements. For 2 weeks, the dogs didn't show any improvement. Consequently, the dogs were treated with electroacupuncture (EA) and Duhuojisheng-tang (DHJST). All the dogs got back the deep pain and presented wagged tail on 14-35 days after starting EA with DHJST. Especially, two of 3 dogs recovered almost normal ambulation and capacities of urination and defecation. But, one dog failed to regain normal ambulation due to inflammation of operative site which is thought to be caused by the bone cement. From these cases, it was thought that the combination of EA and DHJST mightbe one of the suitable therapies in dogs with no neurological improvement.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.128-135
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• 2016

This study examined radiation damage and the optimal gamma-ray dose for mutation breeding in oat (Avena sativa L. cv. Samhan). The seed germination rate decreased as the dose increased over 500 Gy. The median lethal dose (LD₅₀) was approximately 392 Gy. The median reduction dose (RD₅₀) for plant height, tiller number, root length, and flash weight was 411, 403, 394, and 411 Gy, respectively. The optimal dose of gamma irradiation for inducing oat mutation appears to be in the range 300-400 Gy. We performed the comet assay to observe nuclear DNA damage induced by gamma-ray irradiation. This assay showed a clear difference with gamma-ray treatments. DNA damage increased temporarily 7 days after treatment depending on the dose, while no significant difference was identified in response to 300 Gy 30 days after the gamma-ray treatments. The growth characteristics of the M₂ generation decreased as the dose increased over 400 Gy.

• Korean Journal of Environment and Ecology
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• v.6 no.1
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• pp.72-85
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• 1992

The analysis of visitor's behavior in Hallasan National Park was executed for a month in september, 1990. In the characteristics of trip purpose, the nature-oriented motive was 68.9% of the total. And in the characteristics of the accompanied, the ratio of such items as company, friends,2-3 persons and above 12 persons was generally high. The ratio of lunch box was 62.7％ and the item of bring back waste treatment was 69.6% in the characteristic of activity. The degree of landscape satisfaction was 3.98 and comparatively high on considering the maximum degree of 5.0, but in the degree of facility, the degree of dissatisfaction was high. Therefore the degree of total satisfaction was 3.43. In the opinion of users, the entrance by order of arrival, the adoption of reserved system and the limitation of the entrance of a group were gen- orally objected but the adoptation of nature rest-rotation system was comparatively assented Specially, visitor center was rarely used but many visitor who has attended there positively think about it. In factor analysis, 4 factors were extracted. Facilitate, social. user behavior and natural property were the most affecting factor to psychological satisfaction test. Multiple regression results showed that cleanness, land-scape, safety, no. of wastebasket and crowdness items affected the total satisfaction variable in total and local area.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.338-344
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• 1992

This test has been carried out to clear the effectiveness of pyrethroid insecticides to the Diamond back moth, that was complained by a farmer. The results obtained are summarized as follows. Effectiveness of Pyrethroids to suceptable strain and the results of chemical anaysis on the ingredients of pyrethroikds were normal at recommended concentrations. The effect ualue of pyrethroid by which the blocks were only one time treated was 57-59%, whereas the blocks sprayed 4 consecutive times of pyrethroid didn't show any effectiveness as shown in larval increasing rate of 489-552%. Among the results of field test conducted from 1990 to 1991 at 7 area, Dae Gu area showed the highest Effectiveness as 95-98%. However, Pyong Taek area, showed the least effectiveness as 0% in effect value. The resistant population which was collected at Tae kwan Ryung area was resurrected susceptability as much as JMC, in case of Placing it in non-treatment environment, whereas the pupulation which was revealed to pyrethroids for 6 genetations showed as much as 341-544 times of resistance compared with JMC. The non-effectiveness of pyrethroids to Diamondback moth which was complainted by a certain farmer named Mr Ju, was clearly resulted by the development of insect resistance. In Korea, the resistance of Diamondback moth to pyrethroids has developed in some area, as well as the resistance of pyrethroids has developed according to the continuous use of Insecticides, and the susceptability resurrected by stopping the use of pyrethroids. The result from this test would suggest that the pyrethroid insecticides should be applied in turb with other insecticides.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.333-340
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• 2011

This study was conducted on the effect of plant growth retardants (PGRs) on growth of potted Ardisia pusilla and Ardisia pusilla var. variegata grown for a short term in an ebb and flow system. Plants were planted in 10 cm plastic pots, containing a mixture of peat moss and perlite (1 : 1, v/v) on 30 June 2005 and were grown until 23 Sep. 2005. The PGRs used were 50, 200, 350, 500 $mg{\cdot}L^{-1}$ daminozide (B- 9), 10, 40, 70, 100 $mg{\cdot}L^{-1}$ paclobutrazol (Boundy), and 5, 15, 25, 35 $mg{\cdot}L^{-1}$ ethephon (Florel). On every irrigation, the nutrient solution containing PGRs was supplied at a 2 cm depth and was kept for 15~20 minutes. The surplus nutrient solution was drained back into the tank for next irrigations. In both cultivars, paclobutrazol gave the most pronounced effect in inhibition of stretchiness. Ardisia pusilla, which was pinched just before initiation of paclobutrazol treatment, resulted in the greatest inhibition of shoot elongation. Daminozide treatments above 200 $mg{\cdot}L^{-1}$ also gave reduction of shoot length. However, ethephon treatments gave no dwarfing effect in Ardisia pusilla. Inhibition of stretchiness was observed even in the lowest concentration treatments. Among the PGRs, paclobutrazol was the most effective in suppressing plant stretchiness.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.36 no.3
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• pp.111-115
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• 2014

Purpose: This study evaluated powdered burn wound dressing materials from wild silkworm fibroin in an animal model. Methods: Fifteen rats were used in this experiment. Full-thickness $2{\times}2cm$ burn wounds were created on the back of rats under anesthesia. In the two experimental groups, the wounds were treated with two different dressing materials made from silkworm fibroin. In the Control Group, natural healing without any dressing material was set as control. The wound surface area was measured at five days, seven days and 14 days. Wound healing was evaluated by histologic analysis. Results: By gross observation, there were no infections or severe inflammations through 14 days post-injury. The differences among groups were statistically significant at seven days and 14 days, postoperatively (P<0.037 and 0.001, respectively). By post hoc test, the defect size was significantly smaller in experimental Group 1 compared with the Control Group and experimental Group 2 at seven days postoperatively (P=0.022 and 0.029, respectively). The difference between Group 1 and Group 2 was statistically significant at 14 days postoperatively (P<0.001). Group 1 and control also differed significantly (P=0.002). Group 1 showed a smaller residual scar than the Control Group and Group 2 at 14 days post-injury. Histologic analysis showed more re-epithelization in Groups 1 and 2 than in the Control Groups. Conclusion: Burn wound healing was accelerated with silk fibroin spun by wild silkworm Antheraea pernyi. There was no atypical inflammation with silk dressing materials. In conclusion, silk dressing materials can be used for treatment of burn wound.

• Journal of Agricultural Extension & Community Development
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• v.3 no.2
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• pp.197-209
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• 1996

Bangladesh that is known as the poorest country of the world has the large land of fertile soil and very good environment to live, even though we think she may be very bad to live. People being habituated to that good environment, the population explosion made the people to be starven, but the treatment has been simple support without deep analysis of the root of poverty. As the result it is general that the poverty is severer and severer in spite of continuous support. For the last century Korea changed from the country of poverty and despair to that of development and hope, to be model mid vision of under development countries including Bangladesh. At this point it is necessary to look back the way of development to help them to solve their poverty problem. That is the goal of this project, and this thesis is the result of it. It is evaluated for the 1st 5 years work of the project to apply the result to the next 5 years plan. This project had been proceeded from 1. Jul. 1990 to 30. Jun. 1995 at Chilmari area of Bangladesh, with teaching and training to wake up the mentality of the people, demonstration farming and cooperative association. It was proceeded as the Agriculture project of KDAB(Korean Development Association in Bangladesh, an NGO registered to Bangladesh) supported by Good Neighbors, KOICA and many churches and individuals. Especially the teaching and training work was proceeded as the branch of the Canaan Farmer`s School of Korea with instruction and support. After the basic survey for project, the confirmation of working place and the preparation of training facility, 10 times of long term training(3 months worse, boarding) was completed, with many times of short term training, informal training, demonstration farm, various demonstrating works. The results of the work are understanding of the not of problem by participatory observation, making plan to solve it, getting the trust from the people and beginning the practical work with the trainees for development of economy and living situation. The biggest problem of Bangladesh is being understood losing the desire to solve the poverty problem and the self-confidence to be able to do it. It is the conclusion that after solving the problem of thought and mentality, the education, technology and money can be effective for development. So the 1st 5 year project is evaluated as the basic work to analyse the root of problem, to chance mentality of people and to search income source. The next goal is to promote practical living level of people. For that it is necessary to develope die mentality of people including responsibility and self-confidence by teaching and training, to educate cooperative association and technology for economical development, to proceed integrated rural development work with economical development, educational improvement and reformation of environment. It is very important result that they decided to grow poultry for main economical source of Chilmari area, to do economical development work by joint operation of poultry through cooperative association.

• 한국과학기술학회:학술대회논문집
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• 2015.12a
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• pp.247-278
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• 2015

Recently, as DNR prevails more and more in Korea, discussions regarding meaningless medical life-sustaining-treatment (LST) intensified. Some of the Supreme Court decisions are even discussed in mass media, causing public debates. These cases tell us that, as life-sustaining medical technologies are highly developed, more sociological and policy-related analyses are needed on them. Firstly, this study will review 40 previous studies that analyze recent discussions in Korea about LST. Secondly, this study also shows that in bioethical and policy-related perspectives, governance about LST calls for a new implications regarding thanatoethics and thanatopolitics. In this new theoretical framework, death with dignity (DwD) can be understood as a process of giving back the thanatopower to the subject who chooses his way of ending based on his sound and free will. Thirdly, some of the new LST or resuscitation technologies such as automated external defibrillators (AED) are developed in RRI framework. However, if subjects themselves choose not to apply those technologies on them, as in the case of DNR (do not resuscitate) vows, meaning of developing such technologies are to be questioned. But currently such questions regarding the limitations of RRI are seldom asked. I argue that in order to properly apply RRI framework on existing technology, we also need to consider these points.