• 한국미생물·생명공학회지
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• 제17권2호
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• pp.109-114
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• 1989

150개의 토양시료로부터 두유 응고효소를 생산하는 세균 17균주를 분리하였으며 이들 균주는 Bacillus cereus(8균주), Bacillus pumilus(8균주) 및 Bacillus licheniformis(1균주)로 동정하였다. 분리된 두유 응고효소 생산균주중 Bacillus pumilus 118 및 Bacillus licheniformis 192균주가 비교적 높은 두유응고효소 활성을 나타내었고 이들 두 균주의 효소활성은 pH6.0~7.0의 범위에 있어서 pH7.0에 가까울수록 저하되며 그 최적온도는 $65^{\circ}C$였다.

• The Plant Pathology Journal
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• 제37권3호
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• pp.243-257
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• 2021

Bacillus pumilus is the causal agent of trunk bulges disease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic relationships of 20 isolates of B. pumilus group from four rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang). Multi rep-PCR results revealed the genetic profiling among the B. pumilus group isolates, while MLSA results showed 98-100% similarity across the 20 isolates of B. pumilus group species. These 20 isolates, formerly established as B. pumilus, were found not to be grouped with B. pumilus. However, being distributed within distinctive groups of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates close to B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. This is the first MLSA and rep-PCR study on B. pumilus group, which provides an in-depth understanding of the diversity of these rubber-pathogenic isolates in Malaysia.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.157-162
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• 1999

Bacillus pumilus PJ19 isolated from Pinus leaves showed optimum xylanase production when grown in yeast tryptone broth at $37^{\circ}C$, pH 7.2, and shaken at 200 rpm after 48 h of incubation. Xylanase production was induced by xylan and xylose but repressed in the presence of glucose. Xylanase production by B. pumilus PJ19 was not growth-associated and the maximum enzyme production was found after 36 h of incubation.

• 한국미생물·생명공학회지
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• 제48권1호
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• pp.48-56
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• 2020

This study aimed to optimize the culture conditions to improve the crude biosurfactant activity of Bacillus pumilus IJ-1, using a 3³ full-factorial design of response surface methodology (RSM). It was found that submerged fermentation of B. pumilus improved the activity of the crude biosurfactant. The factors selected for optimization were NaCl concentration, temperature, and tryptone concentration. Response surface analysis revealed that the fitted quadratic model was statistically significant and produced an adequate R² value (0.9898) and a low probability value (<0.0001). The optimum level for each factor was found to be 0.567% (w/v) NaCl, 21.851℃ and 0.765% (w/v) tryptone, respectively. Crude biosurfactant activity was found to be most affected by tryptone concentration; then temperature, and finally NaCl concentration. Our results may potentially facilitate large-scale biosurfactant production from B. pumilus IJ-1.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.59-68
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• 2023

Xylanase is an industrially relevant enzyme used for the production of xylobiose and xylose. Various methods are used to enhance the microbial yield of xylanase. In the present study, co-culturing of Bacillus subtilis and Bacillus pumilus were investigated using submerged fermentation for xylanase production, which was markedly increased when sal, sagwan, newspaper, wheat bran, and xylan were used as single carbon sources. Maximum xylanase production was reported after 5 days of incubation in optimized media at pH 7.0 and 37℃, resulting in 2.69 ± 0.25 µmol/min by coculture. The 1:1 ratio of sal and sagwan in optimized production media was shown to be suitable for xylanase synthesis in submerged fermentation (SMF). In comparison to mono-culture using B. pumilus and B. subtilis, co-culturing resulted in an overall 3.8-fold and 2.15-fold increase in xylanase production, respectively.

• Journal of Applied Biological Chemistry
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• 제58권1호
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• pp.81-88
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• 2015

Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배지 조성의 특성을 조사하기 위하여 다양한 탄소원, 질소원 및 무기염이 미치는 영향을 검토하였다. 그 결과 B. pumilus IJ-1은 0.5%(w/v) tryptone을 첨가하여 배양하였을 때 가장 많은 생물계면활성제를 생산하였다. B. pumilus IJ-1은 0-10% (w/v)의 NaCl 농도와 pH 5-10, $20-45^{\circ}C$의 환경에서 생장이 가능하였다. 그리고 생물계면활성제 생산은 pH 9.0의 초기 배지에서 $20^{\circ}C$, 72시간 동안 배양했을 때 최대였으며, 이러한 배양 조건 하에서 얻어진 배양액의 표면장력은 27.0 dyne/cm이었다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.661-667
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• 2013

Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen. Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be $40^{\circ}C$ and pH 9. Lipase BPL1 and lipase BPL2 were stable up to $30^{\circ}C$, whereas lipase BPL3 was stable up to $20^{\circ}C$. Lipase BPL2 was stable within a pH range of 6-10, whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward p-nitrophenyl caprylate ($C_8$). In addition, lipase BPL1 showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and medium-chain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.

• 한국토양비료학회지
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• 제49권4호
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• pp.401-408
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• 2016

The present study described the isolation of 2-methylbutyric acid (2-MBA) produced from Bacillus pumilus L1, to subsequently investigate its nematicidal activity for the control of the root-knot nematode. The results showed that 2-MBA could be purified by chromatographic techniques and was identified using nuclear magnetic resonance and liquid chromatography-mass spectrometry. Crude extract and partially purified compounds had a significant effect on the inhibition of egg hatchability and second-stage juvenile (J2) mortality. A dose-dependent effect of 2-MBA was observed for J2 mortality and egg hatchability. Egg hatchability was 69.2%, 59.9%, 32.7%, and 0.0% at 125, 250, 500, and $1000{\mu}g\;mL^{-1}$ of 2-MBA after 4 d of incubation, respectively. Meanwhile, J2 mortality was in the range of 24.4%-100.0% after 2 d of incubation, depending on the concentrations of 2-MBA used. A pot experiment also demonstrated that treatment of B. pumilus L1 culture caused a significant reduction in the number of galls, egg masses, and J2 population than that of the tap water (TW) control. However, as the B. pumilus L1 culture concentration was decreased, the efficacy of nematode control by treatment of B. pumilus L1 culture was reduced compared to that of TW. B. pumilus L1 inoculation at different concentrations also promoted cucumber plant growth. Therefore, our study demonstrated the potential of 2-MBA from B. pumilus L1 as a biocontrol agent against the root-knot nematode and a plant growth promoter for cucumber plants.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.370-377
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• 1999

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{\circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{\circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{\circ}C$, and was quite stable from pH5.0 to 10.0.

• 한국미생물·생명공학회지
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• 제32권4호
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• pp.291-296
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• 2004

고품질, 기능성 청국장 제조를 위한 균주 개발 연구의 일환으로, 재래식 청국장으로부터 생육이 빠르며, 면역증강활성이 우수한 JB-1 균주는 Bacillus pumilus 로 확인되었으며 Bacillus pumilus KCTC 10461BP로 균주기탁 되었다. JB-1으로 발효시킨 청국장은 관능성과 영양성이 우수하며 물추출물 2mg/ml의 농도에서 410% 정도의 면역세포 증식율을 나타내었으며, 산업적 규모의 6 kg 의 대두증자에 20 ml 배양액을 접종함으로서 16시간 이내에 저이취와 높은 생균수 및 아미노산 함량의 청국장 제조가 가능함을 확인하였다.