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http://dx.doi.org/10.3839/jabc.2015.014

Characteristics of Culture Conditions for the Production of Biosurfactant by Bacillus pumilus IJ-1  

Park, Eunjin (Department of Neuroscience, Graduate School, Inje University)
Kim, Jiyeon (College of General Education, Inje University)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.1, 2015 , pp. 81-88 More about this Journal
Abstract
To characterize the culture medium for the biosurfactant production by Bacillus pumilus IJ-1, the influences of various carbon, nitrogen and mineral sources were assessed. As a result, the highest biosurfactant production was observed after 96 h cultivation containing 0.5% (w/v) tryptone. The strain was able to grow and produce biosurfactant at 0-10% (w/v) NaCl, in the pH range of 5-10, and at $20-45^{\circ}C$. Optimal culture conditions for the biosurfactant production were at $20^{\circ}C$ and pH 9.0 after 72 h incubation and the surface tension of biosurfactant was 27.0 dyne/cm.
Keywords
Bacillus pumilus IJ-1; biosurfactant; culture conditions; medium composition; surface tension;
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