• Title/Summary/Keyword: Bacillus pumilus

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Isolation and Identification of Bacteria Producing a Soybean Milk Clotting Enzyme (두유 응고효소 생산균의 분리 및 동정)

  • 하덕모;이철우
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.109-114
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    • 1989
  • Seventeen bacterial strains producing an extracellular soybean milk clotting enzyme were Isolated from 150 soil samples, and identified as Bacillus cereus(8 strains), Bacillus pumilus(8 strains) and Bacillus licheniformis (1 strain). Among them, Bacillus pumilus strain 118 and Bacillus licheniformis strain 192 showed relatively high soybean milk clotting activity. The coagulability of enzymes from these strains decreased as the pH of soybean milk was increased from 6.0 to 7.0. The optimum temperature for soybean milk clotting activity was $65^{\circ}C$.

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Current Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches

  • Husni, Ainur Ainiah Azman;Ismail, Siti Izera;Jaafar, Noraini Md.;Zulperi, Dzarifah
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.243-257
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    • 2021
  • Bacillus pumilus is the causal agent of trunk bulges disease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic relationships of 20 isolates of B. pumilus group from four rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang). Multi rep-PCR results revealed the genetic profiling among the B. pumilus group isolates, while MLSA results showed 98-100% similarity across the 20 isolates of B. pumilus group species. These 20 isolates, formerly established as B. pumilus, were found not to be grouped with B. pumilus. However, being distributed within distinctive groups of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates close to B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. This is the first MLSA and rep-PCR study on B. pumilus group, which provides an in-depth understanding of the diversity of these rubber-pathogenic isolates in Malaysia.

Characterization of Xylanase Produced by Bacillus pumilus Strain PJ19

  • Hamzah, Ainon;Abdulrashid, Nooraini
    • Journal of Microbiology and Biotechnology
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    • v.9 no.2
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    • pp.157-162
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    • 1999
  • Bacillus pumilus PJ19 isolated from Pinus leaves showed optimum xylanase production when grown in yeast tryptone broth at $37^{\circ}C$, pH 7.2, and shaken at 200 rpm after 48 h of incubation. Xylanase production was induced by xylan and xylose but repressed in the presence of glucose. Xylanase production by B. pumilus PJ19 was not growth-associated and the maximum enzyme production was found after 36 h of incubation.

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Optimization Using 33 Full-Factorial Design for Crude Biosurfactant Activity from Bacillus pumilus IJ-1 in Submerged Fermentation

  • Kim, Byung Soo;Kim, Ji Yeon
    • Microbiology and Biotechnology Letters
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    • v.48 no.1
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    • pp.48-56
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    • 2020
  • This study aimed to optimize the culture conditions to improve the crude biosurfactant activity of Bacillus pumilus IJ-1, using a 33 full-factorial design of response surface methodology (RSM). It was found that submerged fermentation of B. pumilus improved the activity of the crude biosurfactant. The factors selected for optimization were NaCl concentration, temperature, and tryptone concentration. Response surface analysis revealed that the fitted quadratic model was statistically significant and produced an adequate R2 value (0.9898) and a low probability value (<0.0001). The optimum level for each factor was found to be 0.567% (w/v) NaCl, 21.851℃ and 0.765% (w/v) tryptone, respectively. Crude biosurfactant activity was found to be most affected by tryptone concentration; then temperature, and finally NaCl concentration. Our results may potentially facilitate large-scale biosurfactant production from B. pumilus IJ-1.

Assessment and Optimization of Xylanase Production Using Mono-Culture and Co-Cultures of Bacillus subtilis and Bacillus pumilus

  • Chitranshu Pandey;Neeraj Gupta
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.59-68
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    • 2023
  • Xylanase is an industrially relevant enzyme used for the production of xylobiose and xylose. Various methods are used to enhance the microbial yield of xylanase. In the present study, co-culturing of Bacillus subtilis and Bacillus pumilus were investigated using submerged fermentation for xylanase production, which was markedly increased when sal, sagwan, newspaper, wheat bran, and xylan were used as single carbon sources. Maximum xylanase production was reported after 5 days of incubation in optimized media at pH 7.0 and 37℃, resulting in 2.69 ± 0.25 µmol/min by coculture. The 1:1 ratio of sal and sagwan in optimized production media was shown to be suitable for xylanase synthesis in submerged fermentation (SMF). In comparison to mono-culture using B. pumilus and B. subtilis, co-culturing resulted in an overall 3.8-fold and 2.15-fold increase in xylanase production, respectively.

Characteristics of Culture Conditions for the Production of Biosurfactant by Bacillus pumilus IJ-1 (Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배양 특성)

  • Park, Eunjin;Kim, Jiyeon
    • Journal of Applied Biological Chemistry
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    • v.58 no.1
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    • pp.81-88
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    • 2015
  • To characterize the culture medium for the biosurfactant production by Bacillus pumilus IJ-1, the influences of various carbon, nitrogen and mineral sources were assessed. As a result, the highest biosurfactant production was observed after 96 h cultivation containing 0.5% (w/v) tryptone. The strain was able to grow and produce biosurfactant at 0-10% (w/v) NaCl, in the pH range of 5-10, and at $20-45^{\circ}C$. Optimal culture conditions for the biosurfactant production were at $20^{\circ}C$ and pH 9.0 after 72 h incubation and the surface tension of biosurfactant was 27.0 dyne/cm.

Isolation and Biochemical Characterization of Bacillus pumilus Lipases from the Antarctic

  • Arifin, Arild Ranlym;Kim, Soon-Ja;Yim, Joung Han;Suwanto, Antonius;Kim, Hyung Kwoun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.5
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    • pp.661-667
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    • 2013
  • Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen. Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be $40^{\circ}C$ and pH 9. Lipase BPL1 and lipase BPL2 were stable up to $30^{\circ}C$, whereas lipase BPL3 was stable up to $20^{\circ}C$. Lipase BPL2 was stable within a pH range of 6-10, whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward p-nitrophenyl caprylate ($C_8$). In addition, lipase BPL1 showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and medium-chain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.

Identification of 2-methylbutyric Acid as a Nematicidal Metabolite, and Biocontrol and Biofertilization Potentials of Bacillus pumilus L1

  • Lee, Yong-Seong;Cho, Jeong-Yong;Moon, Jae-Hak;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.4
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    • pp.401-408
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    • 2016
  • The present study described the isolation of 2-methylbutyric acid (2-MBA) produced from Bacillus pumilus L1, to subsequently investigate its nematicidal activity for the control of the root-knot nematode. The results showed that 2-MBA could be purified by chromatographic techniques and was identified using nuclear magnetic resonance and liquid chromatography-mass spectrometry. Crude extract and partially purified compounds had a significant effect on the inhibition of egg hatchability and second-stage juvenile (J2) mortality. A dose-dependent effect of 2-MBA was observed for J2 mortality and egg hatchability. Egg hatchability was 69.2%, 59.9%, 32.7%, and 0.0% at 125, 250, 500, and $1000{\mu}g\;mL^{-1}$ of 2-MBA after 4 d of incubation, respectively. Meanwhile, J2 mortality was in the range of 24.4%-100.0% after 2 d of incubation, depending on the concentrations of 2-MBA used. A pot experiment also demonstrated that treatment of B. pumilus L1 culture caused a significant reduction in the number of galls, egg masses, and J2 population than that of the tap water (TW) control. However, as the B. pumilus L1 culture concentration was decreased, the efficacy of nematode control by treatment of B. pumilus L1 culture was reduced compared to that of TW. B. pumilus L1 inoculation at different concentrations also promoted cucumber plant growth. Therefore, our study demonstrated the potential of 2-MBA from B. pumilus L1 as a biocontrol agent against the root-knot nematode and a plant growth promoter for cucumber plants.

isolation of Xylanase-producing Thermo-tolerant Bacillus sp. and Its Enzyme Production (Xylanase를 생산하는 내열성 Bacillus속 균주의 분리와 효소생산 조건)

  • 박영서;강미영;장학길;박귀근;강종백;이정기;오태광
    • Microbiology and Biotechnology Letters
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    • v.27 no.5
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    • pp.370-377
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    • 1999
  • Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{\circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{\circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{\circ}C$, and was quite stable from pH5.0 to 10.0.

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Isolation of Immuno-stimulating Strain Bacillus pumilus JB-1 from Chungkook-jang and Fermentational Characteristics of JB-1 (청국장으로부터 면역증강활성이 우수한 Bacillus pumilus JB-1의 분리 및 분리균의 청국장 발효특성)

  • Kim, Yeong-Suk;Gwon, Jeong-Suk;Gwon, Gi-Seok;Son, Ho-Yong;Gwon, Ha-Yeong
    • Microbiology and Biotechnology Letters
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    • v.32 no.4
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    • pp.291-296
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    • 2004
  • To produce functional and high nutritional chungkook-jang, an immuno-stimulating and rapid growing bacterium was isolated from one of Korean traditional food, chungkook-jang. The isolated bacterium was identified as Bacilpumilus and deposited in Korean Collection for Type Culture (KCTC 10461BP). The chungkook-jang fermented by JB-l has good taste and pleasant smell with high content of free amino acids, and the water extract of chungkook-jang showed 410% of immuno-stimulation activity at concentration of 2 mg/ml. The rapid fermentation in 16 h is achieved by inoculation of 20 ml culture broth of JB-l into 6 kg cooked soybean in commercial scale at $40^{\circ}C$.