This study was designed to observe the adaptive changes of mandibular condyles to displacement of mandibular condyle in adult animals. In this study, 16 rabbits weighting about 3.5 kg was selected. Four rabbits were preserved for control group and 8 animals were divided into 3 groups, 2 weeks, 4 weeks and 8 weeks. The experimental animals were performed oblique osteotomy on both mandibular ramus and internal wiring at mandibular border. The experimental animals were sacrificed consecutively on the 2 weeks, 4 weeks and 8 weeks after oblique osteotomy and mandibular condyles were dissected out carefully to produced tissue specimen. The specimens were fixed with 10% N formaline solution for 24 hours and rinsed with phosphate buffer solution. It was decalcified with 5% nitric acid for 15 days. Thereafter the specimens were dehydrated in alcohol series and embedded paraffin as usual manner. The mebedded specimen were sectioned in $4-6{\mu}m$ microtome, stained with hematoxylin-eosin and azan stain and observed through light microscope. The following results were observed from this experiment. When there was postional change of condyle head after mandibular ramus oblique osteotomy in adult rabbit, 1. The density of chondrocyte was generally increased at condylar cartilage and the thickness of condylar cartilage was increased posterosuperior aspect of the mandibular condyle slightly. 2. The density of chondrocyte was increased at proliferative zone so fibrous articular zone, porliferative zone and hypertrophic zone was clearly distinguished. 3. Active endochondral bone formation was occurred at mandibular condyle.
This study examined the key factors that significantly improved the demand of multi-habitation. It determined the factors at the macroscopic level (or push factors) and the microscopic level (or pull factors). Focusing on a microscopic viewpoint, this study looked at the process of settlement through investigating 78 MH residents in the Seoul metropolitan area. The survey included the questions, such as who they are, how they prepared for moving, and how much they enjoyed their rural lives. In addition, any differences in this process were analyzed depending on respondents' characteristics. Major findings are as follows: First, general macro-level circumstances seemed supportive for the MH lifestyles. Second, six keywords were determined to represent the recent MH trends. They are "semi-sedentism, clustering, young people, female, money, and policy". Third, the distances between the original towns for native residents and new second-home towns for MH residents affected the interactions among them. However, these two groups had better relationships when the second-home towns were apart from the original towns. I then considered the need of a buffer zone between the two residential areas for MH residents. The conceptual difference between MH residents (i.e., semi-sedentism) and original rural residents (i.e., sedentism) might require certain types of buffer zones to continue good relationships among them.
To find out the status and characteristics of exotic plants in the Gwangreung Forest, a representative forest ecosystem in central Korea, we surveyed the species composition, coverage, and habitat illuminance of exotic plant species in a set of quadrats established along roads and trails in and around the Gwangreung Forest and buffer zone areas, In 1932, only five species of exotic plants were found along paths and roads in the Gwangreung Forest. However, the number of species in the forest has dramatically increased since 2000, when Ambrosia artemisiifolia L. and A. trifida L. were first recorded, and in 2007, 38 species of exotic plants were recorded. Among the 11 families, 23 genera and 25 species of exotic plants recorded in the quadrats, Compositae was the most common family (11 species), and perennial herbs, 42%, were the most frequently occurring life type, followed by annual herbs (31%) and biennial herbs (19%). Plants of North American origin comprised 48% of exotic species identified in our surveys. Exotics were found most frequently in quadrats along roads in the forest, followed by the buffer zones and hiking trails. The number of species and individuals of exotic plants decreased as we moved deeper into the forest, but Aster pilosus Willd. and Erigeron annuus (L.) Pers. were identified along hiking trails in the interior, and appeared to be capable of spreading further and more rapidly into the forest than other species.
The cariostatic effect of fluoride had been established by many epidemiologic and experimental studies. But, there are still different views on the mechnism of cariostasis and remineralization, especially about the proper fluoride concentration. The purpose of this study is to ascertain the remineralization of caries lesion and influence of fluoride concentration which affect remineralization by a study based on dynamic mechanism. The subjects, sound permanent teeth without demineralization or crack, were immersed in lactic acid buffered demineralization solution for 4 days. Dental caries with surface zone and subsurface lesion were artificially produced. All specimens were immersed in lactic acid buffered remineralization solution which had fluoride concentrations of 1 ppm, 2 ppm, 3 ppm for 10days. Final conclusions were obtained by observing the specimens for every 10 days under polarized microscopy. 1. Remineralization of caries lesion as well as demineralization of enamel were produced by changing the degree of saturation of lactic acid buffer solution. 2. Remineralization of caries lesion was facillitated by fluoride ion in lactic acid buffer solution. but, remineralization of the entire caries lesion was not increased as fluoride ion concentration increased.
Amongst the various antimicrobial peptides, lysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. Here we propose the biosynthesis and refolding of recombinant lysozyme in Escherichia coli expressed in inclusion body form. The Agrius lysozyme gene was amplified using gene specific primers and then ligated into the pGEX-4T-1 vector, which contained the glutathione S-transferase (GST) gene as a fusion partner. A recombinant lysozyme was expressed in E. coli Rosetta cells using a pGEX-4T-1 expression vector, and the fusion protein was induced by ioporpyl-${\beta}$-D-thiogalactopyranoside (IPTG). The recombinant protein produced as an inclusion body was resolubilized in solubilization buffer, and the resultant solution was dialyzed in refolding buffer. After thrombin cleavage, the recombinant lysozyme was purified by ion exchange chromatography and reverse phase chromatography. The recombinant lysozyme was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoreactivity against the anti-Agrius lysozyme was observed by western blot analysis of this protein. The recombinant lysozyme displayed antibacterial activity against Bacillus megaterium and Micrococcus luteus, which was confirmed by the inhibition zone assay.
Kim, H.S.;Oh, S.S.;Ha, D.W.;Ha, H.S.;Ko, R.K.;Moon, S.H.
Progress in Superconductivity and Cryogenics
/
v.14
no.1
/
pp.1-3
/
2012
LMO($LaMnO_3$) buffer layer of superconducting coated conductor was deposited on IBAD-MgO template in the plasma atmosphere at $650^{\circ}C$ which is relatively low compared with conventional deposition temperature of more than $800^{\circ}C$. Deposition method of LMO was DC sputtering, and target and deposition chamber were connected to the cathode and anode respectively. When DC voltage was applied between target and chamber, plasma was formed on the surface of target. The tape substrate was located with the distance of 10 cm between target and tape substrate. When anode bias was connected to the tape substrate, electrons were attracted from plasma in target surface to the tape substrate, and only tape substrate was heated by electron bombardment without heating any other zone. The effect of electron bombardment on the surface of substrate was investigated by increasing bias voltage to the substrate. We found out that the sample of electron bombardment had the effect of surface heating and had good texturing at low controlling temperature.
Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
/
v.17
no.1
/
pp.75-94
/
2019
As the present single-layer repository concept requires too large an area for the site of the repository, a multi-layer repository concept has been suggested to improve the disposal density. The effects of the excavation damaged zone around the multi-layer repository constructed in the deep host rock on the temperature distribution in the repository were analyzed. For the thermal analysis of the multi-layer repository, the hydrothermal model was used to consider the resaturation process occurring in the buffer, backfill and rock. The existence of an excavation damaged zone has a significant effect on the temperature distribution in the repository, and the maximum peak temperatures of double-layer and triple-layer repositories can rise to $7^{\circ}C$ and $12^{\circ}C$, respectively depending on the size of the excavation damaged zone and the degree of decrease of the thermal conductivity. The dominant factor affecting the peak temperature in the multi-layer repository is the decrease of thermal conductivity in the excavation damaged zone, and the excavation damaged zone formed around the deposition hole has more significant effects on the peak temperature than does the excavation damaged zone formed around the disposal tunnel.
KSCE Journal of Civil and Environmental Engineering Research
/
v.43
no.6
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pp.735-747
/
2023
In recent times, the sharp increase in extreme flood damages due to climate change has posed a challenge to effectively address flood-related issues solely relying on conventional flood management infrastructure. In response to this problem, this study aims to consider the effectiveness of nature-based flood management approaches, specifically levee retreat and relocation. To achieve this, we utilized a 1D numerical model, HEC-RAS, to analyze the flood reduction effects concerning floodwater levels, flow velocities, and time-dependent responses to a 100-year frequency flood event. The analysis results revealed that the effect of creating a flood buffer zone of the nature-based solution extends from upstream to downstream, reducing flood water levels by up to 30 cm. The selection of the flow roughness coefficient in consideration of the nature-based flood buffer space creation characteristics should be based on precise criteria and scientific evidence because it is sensitive to the flood control effect analysis results. Notably, floodwater levels increased in some expanded floodplain sections, and the reduction in flow velocities varied depending on the ratio of the expanded cross-sectional area. In conclusion, levee retreat and floodplain expansion are viable nature-based alternatives for effective flood management. However, a comprehensive design approach is essential considering flood control effects, flow velocity reduction, and the timing of peak water levels. This study offers insights into addressing the challenges of climate-induced extreme flooding and advancing flood management strategies.
Almost all Drivers who are not familiar with local areas usually rely on road signs equipped along the roadways. The road signs in Korea present the name of the city along the driver’s direction. The consistency of guided name on the road-signs is important for drivers. The discordances among road signs frustrate drivers particularly when the drivers are confused with whether or not they are in the right direction. There are several studies focusing on the continuity of information on the road signs. Most of the researches, however, do not suggest the objective way but diagnose present problem. Applying the Analytic Hierarchy Process (AHP), we evaluate the impact of information on road signs and select the candidate information considering the score and limited number of information. We also suggest the reasonable spatial influence area of road sign information using geo-spatial analysis. From this study, we expect that the director in charge of selecting information can make decision reasonably without difficulties of choosing information.
This study was carried out to suggest the basic data of planting method for construction of buffer green space based on the land use in case of reclaimed land by analyzing land structure, planting concept, and planting structure in buffer green space, Rokko Island, Kobe, Japan. Rokko Island(total area: 580ha) is divided into port and logistics industry area and urban area by constructing the box type large-scale buffer green space. The land structure of buffer green space were biased mounding type, parallel mounding type, and complex mounding type. The width of buffer green space was 50meters in case of northern area, from 28 to 32meters in case of eastern area, and 37.5meters in case of western area, and the slope of that was from 18 to 25 degrees and the height of that was from 2 to 15meters. There were applied landscape and buffer planting concept on the sea side area of northern buffer green space, on the other hand landscape and shade planting concept on the Inner city side area of that. According to the result of planting structure analysis of northern buffer green space, the main woody species were those of deciduous-evergreen species grow in warm-temperate forest zone such as Quercus glauca, Cinnamomum camphora, Machilus thunbergii, Elaeagnus maritima. The results of maximum number of species and planting density by $100mm^2$ was that 9 species 22 individuals in canopy layer, 9 species 15 individuals in understory layer, 3 species 67 individuals in shrub layer, and 14 species 104 individuals in total. The plant coverage of northern buffer green space based on the ecological planting method was from 69 to 139% in case of canopy layer, from 26 to 38% in case of understory layer, from 6 to 7% in case of shrub layer, and from 101 to 184% in total. Index of plant crown volume of northern buffer green space based on the ecological planting method was from 1.40 to $3.12m^3/m^2$ in case of canopy layer, from 0.43 to $0.55m^3/m^2$ in case of understory layer, $0.06m^3/m^2$ in case of shrub layer, and from 1.89 to $3.73m^3/m^2$ in total.
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