• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.53-62
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• 2019

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.11
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• pp.1573-1579
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• 2010

This research was performed to determine the antioxidant activity, nitrite scavenging activity, and its inhibitory activity on angiotensin converting enzyme (ACE), xanthine oxidase, $\alpha$-glucosidase, and elastase of hot water extract from pine bud (WPB). Antioxidant activity of WPB was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity. DPPH radical scavenging activity and SOD-like activity of WPB were remarkably increased in a dose-dependent manner, and were about 71.4 and 85.4% at 2 mg/mL, respectively. The xanthine oxidase and ACE inhibitory activities were about 70.9 and 51.9% at 2 mg/mL of WPB, respectively. Nitrite scavenging activity of WPB was about 59.1, 53.8, and 39.5% on pH 1.2, 3.0, and 6.0 at 2 mg/mL, respectively. The WPB also showed elastase and $\alpha$-glucosidase inhibitory effects. These results revealed that pine bud have strong antioxidant activity and positive effects on the inhibition of xanthine oxidase, ACE, and elastase.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.141-145
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• 2013

The aim of this study was to investigate the effect of two male cultivars of asparagus with low different chilling periods on bud breaking, relative spear growth rate, shoot number and yield. Four-month-old plants of two male cultivars of asparagus were dried to impose dormancy and placed in a coldroom at $5^{\circ}C$ to satisfy the chilling requirement for 0, 2, 4, and 6 weeks. After the specified chilling time, the pots were placed in greenhouse. The results showed that mean days to bud break of 'Jersey Giant' significantly decreased with an increasing chilling period from 0 to 6 weeks, but 'Jersey Supreme' was not affected. The relative spear growth rate of 'Jersey Supreme' was significantly stimulated by chilling compared to non-chilled plants. Shoot production and total spear weight of 'Jersey Supreme' tended to be greater than 'Jersey Giant' irrespective of chilling treatments. Following low temperature treatment, 'Jersey Supreme' showed shorter dormant period than 'Jersey Giant', faster growth of spear. The present study showed that asparagus male cultivar at duration of chilling treatment had an affirmative effect on bud breaking, spear growth rates, shoot number and yield of asparagus.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.43-49
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• 1985

This study was conducted to investigate the existance of dormancy in newly collected tubers of Eleocharis kuroguwai Ohwi from paddy fields and to determine the effect of various growth regulators on the bud sprouting and tuberization of E. kuroguwai. The maximum percent sprouting of E. kuroguwai collected at every month during winter was less than 60% regardless of varied collection tunes until 50 days after incubation, suggesting the presence of dormancy in E. kuroguwai tubers. This dormancy was markedly broken as the time went by, probably due to the exposure of tubers the extremly low temperature. The treatment of $BA10^{-3}M$ and $BA10^{-3}+GA10^{-6}M$ increased bud sprouting about 10 and 11 times, respectively, than that of the untreated control indicating that BA seems to be one of the most effective agents among the growth regulators used on the bud sprouting of E. kuroguwai. Foliar application of BA increased tuberization of E. kuroguwai by an average of 34.4% as compared with the untreated control. The highest increase, 39.8% was obtained with BA treatment at $10^{-5}M$.

• Journal of Korean Society of Forest Science
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• v.40 no.1
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• pp.57-62
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• 1978

This study was carried out to determine the proper transplanting time of Platanus occidentalis L. (plane tree, sycamore) with 5 to 6 cm and 25 to 30 cm in diameter of breast height at the forest nursery of Chonnam National University in 1977. For that purpose, the experiment of the time of transplantation, and the moisture content and soluble sugar were analysed. The results are as follows: 1. Both its rooting and growth were slightly different in the period of January to early April (before bud-break), but remarkably declined after its bud-break (mid-April to May). 2. And also, its moisture content and soluble sugar were slightly different (January to early April), but, on the other hand, considerably increased in the content of moisture and sharply decreased in soluble sugar after bud-break (mid-April to May). 3. In comparision with healthy trees, rooting and growth of trees infected with Cankers were unusually retarded and its moisture content and soluble sugar were much less. 4. The proper time to plant sycamore was recognized to be the period of November to March, since the higher amounts of soluble sugar and the lesser amounts of moisture in that period. 5. The sudden exposure to the sun of the boles of diseased and wounded trees could be in death in case of sun-scald on the side of south-west. 6. Pruning wounds should be treated with an antiseptic, as soon as they are made, to prevent entrance of decay or disease while the wound is healing. 7. The wound and sun-scald can be presented by covering the trunk with straw ropes before transplantations.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.387-390
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• 2000

The experiment was conducted to investigate the effects of vine management and yield on fruit quality and bud burst of 'Campbell Early' grapevines in Hwaseong and Suwon area. The extreme value of minimum temperature of Hwaseong area, frequently causing cold damage, was lower than that of Suwon area in winter. Narrow spacing, overbearing, and poor vine management were observed in Hwaseong area, where the harvest time was delayed by 7 days compared with that of Suwon area. Total carbohydrate content of bearing mother branches was lower with 1.2~1.7%, and the percentage of bud burst was extremely lower at $-15^{\circ}C$. In Suwon area, there were no differences in cluster and berry size, but soluble solids content was lower and skin coloration was poorer when yield was high. Total carbohydrate content was lower when yield was high. The percentage of bud burst was lower at cold treatments below $-20^{\circ}C$. The results suggest that appropriate yield based on fruit quality is 2.3~2.6 MT/10a.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.171-179
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• 2004

Hormonal requirements inducing different regeneration pathways with particular emphasis on somatic embryo-genesis in Alhagi graecorum were studied. While combination of 0.5 $\mu{M}$ 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 $\mu{M}$ 6-benzylaminopurine (BAP) and 5 $\mu{M}$ 1-naphthaleneacetic acid (NAA) in MS medium induced callus formation and callus maintenance from internodal explants, each alone or in combination with other induced distinct regeneration pathway. Adventitious bud formation was induced on MS medium supplemented with 2.5 $\mu{M}$ BAP. It was improved when 2.5 $\mu{M}$ BAP was used in combination with 5 $\mu{M}$ NAA. MS medium containing 0.5 $\mu{M}$ 2,4-D or 5 $\mu{M}$ NAA induced the formation of abnormal direct somatic embryos. While increase of 2,4-D concentration (1.125-9) resulted in the formation of viable embryogenic mass, increase of NAA did not change its effect. NAA should be used in combination with 2,4-D even at low concentration (0.5 $\mu{M}$) to form embryogenic mass. In A. gaecorum, the role of 2,4-D as trigger of somatic embryogenesis and BAP as trigger of adventitious bud formation was deduced, but for maximum yield certain auxin-cytokinin ratio should be applied. Embryogenic masses characterized by high water content, low peroxidase activity, and low number of peroxidase and glutamate oxaloacetate transaminase bands in comparison with calli obtained under conditions stimulating adventitious bud formation. The resulted differential gene expression, which could be detected by native-PAGE patterns, could be used as marker for organogenic pathway in A. graecorum.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.151-156
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• 2010

The Objective of this study was to investigate the effect of ethylene exposure and silver thiosulfate (STS) on flower senescence and vase life of cut iris (Iris hollandica). Cut iris flowers were pretreated in 1 mM STS solution for 30 minutes and exposed to 0, $3mL{\cdot}L^{-1}$ethylene for 24 hours. The vase life of iris treated at a bud stage was showed day 4.2 by exposure to $3mL{\cdot}L^{-1}$ ethylene and day 4.6 by exposure to $0mL{\cdot}L^{-1}$ ethylene. The pulsing of STS increased vase life of iris treated at a half-open stage. But, the vase life of iris was not affected by exposure to ethylene. Therefore, iris flowers were not sensitive to ethylene exposure. Iris flowers harvested at a bud stage do not progress to fully open flowers and then show flower senescnce, the optimum harvest stage seems to be a stage when flowers are open to some degree.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.273-277
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• 2005

Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1 $mg/{\ell}\;BA\;and\;2\;mg/{\ell}$ IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1 $mg/{\ell}\;IBA$. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.6-9
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• 2005

Plant biotechnology involving genetic modification has been rather controversial. However, the major issues related to safety are being addressed by continued improvements in technology. Some of the related facts will be highlighted to set the tone for a scientific discussion on the possibilities of using the technology for crop improvement. Our main research interest is to understand the molecular regulation of shoot bud regeneration in plant tissue culture, which is essential for crop improvement by biotechnology. We have isolated and characterized some genes that are associated with adventitious shoot regeneration. These include a MADS-box cDNA (PkMADS1) from paulownia kawakamii, which regulates vegetative shoot development and in vitro shoot regeneration from leaf explants. Another gene we have characterized from petunia codesfor a cytokinin binding protein (PETCBP). Preliminary functional analysis of this gene indicated that this also affects adventitious shoot bud initiation. Also, the antisense suppression of this gene in petunia causedexcessive branching. Results from our work and selected other publications will be used to highlight the possibilities of manipulation of such genes to improve crop species.