• Molecules and Cells
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• v.37 no.2
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• pp.126-132
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• 2014

As a tumor suppressor homologue during mitosis, Chk2 is involved in replication checkpoints, DNA repair, and cell cycle arrest, although its functions during mouse oocyte meiosis and early embryo development remain uncertain. We investigated the functions of Chk2 during mouse oocyte maturation and early embryo development. Chk2 exhibited a dynamic localization pattern; Chk2 expression was restricted to germinal vesicles at the germinal vesicle (GV) stage, was associated with centromeres at pro-metaphase I (Pro-MI), and localized to spindle poles at metaphase I (MI). Disrupting Chk2 activity resulted in cell cycle progression defects. First, inhibitor-treated oocytes were arrested at the GV stage and failed to undergo germinal vesicle breakdown (GVBD); this could be rescued after Chk2 inhibition release. Second, Chk2 inhibition after oocyte GVBD caused MI arrest. Third, the first cleavage of early embryo development was disrupted by Chk2 inhibition. Additionally, in inhibitor-treated oocytes, checkpoint protein Bub3 expression was consistently localized at centromeres at the MI stage, which indicated that the spindle assembly checkpoint (SAC) was activated. Moreover, disrupting Chk2 activity in oocytes caused severe chromosome misalignments and spindle disruption. In inhibitor-treated oocytes, centrosome protein ${\gamma}$-tubulin and Polo-like kinase 1 (Plk1) were dissociated from spindle poles. These results indicated that Chk2 regulated cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development.

• Nuclear Engineering and Technology
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• v.23 no.1
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• pp.56-65
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• 1991

The LOFT LOCE L2-3 was simulated using the RELAP5/MOD2 Cycle 36.04 code to assess its capability in predicting the thermal-hydraulic phenomena in LBLOCA of a PWR. The reactor vessel was simulated with two core channels and split downcomer modeling for a base case calculation using the frozen code. The result of the base calculation showed that the code predicted the hydraulic behavior, and the blowdown thermal response at high power region of the core reasonably and that the code had deficiencies in the critical How model during subcooled-two-phase transition period, in the CHF correlation at high mass flux and in the blowdown rewet criteria. An overprediction of coolant inventory due to the deficiencies yielded the poor prediction of reflood thermal response. Improvement of the code, RELAP5 / MOD2 Cycle 36.04, based on the sensitivity study increased the accuracy of the prediction of the rewet phenomena.

• Journal of the Korean Society of Industry Convergence
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• v.14 no.2
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• pp.59-65
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• 2011

$CO_2$ Welding which uses $CO_2$ instead of inert gas is most widely used in industrial sites. Welding rod for $CO_2$ Welding is roughly divided into solid wire and flux cored wire. $CO_2$ Welding has higher efficiency than any other welding methods, and also economic and speedy to handle, that's why is used frequently for welding general structures. As most of studies about $CO_2$ Welding are focused on metallurgical changes of successful joints, they developed theories about the change of configuration on weld parts. This study is especially focused on not only the change of configuration on weld parts, but also the change of the penetrating depth through changing the position of welding torch. For inspection, applied AWS A5.20 E70-1 among welding wires and fixed moving angles of torch, but controled the values of voltage and the position of welding. Also Automatic Feed Mechanism is used for exact movement of material, specimen is a piece of steel for general structures. By measuring and analyzing the configuration of sliced section and the values of welding leg length and welding throat after welding, the outcome about the changes turned out.

• Journal of the Korean Wood Science and Technology
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• v.6 no.1
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• pp.8-14
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• 1978

In order to determine the feeding value of t:1e waste wood of Lentinus eddodes, the waste wood was milled and treated with $Ca(OH)_2$ and cellulase, then raw waste wood and the treated waste wood were fed to broiler chicken and Korean native goates. The results obtained were as follows; 1. The chemical composition of the waste wood was c. protein 2.26%. c. fat 2.57%. c. fiber 44.60 and c. ash 5.58%. No differences were detected between the raw waste wood and $Ca(OH)_2$ or cellulase treated waste wood in chemical composition. 2. In amino acid composition. no cystine and methionine was showed, but trace amount of Vitamin A, $B_1$, and $B_2$, and cholin chloride were detected. 3. Trichoderma viride SANK. No. 16374 was used to decompose the cellulose. 4. In broiler performance, when waste wood meal was subsituted for 5% wheat bran, no adverse effect was showed on weight gain, feed in take and feed efficiency. But $Ca(OH)_2$ and cellulase treated waste wood meal could be subsituted for 8% wheat bran. 5. In Korean native goat performance, feeding waste wood meal subsituted by 50% various bran (25% defatted rice bran and 25% barley bran) had no adverse effect on growth and feed efficiency. 6. Considering the above results, though the waste wood meal could be replaced to part of various bran, there was a bottle-neck to process the waste wood. The waste wood of Lentinus edodes was little feeding value on bed of ruminant.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.1B
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• pp.24-33
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• 2002

The cell search scheme for W-CDMA consists of the following three stages: slot synchronization(1st stage), group identification and frame boundary detection(2nd stage), and long code identification(3rd stage). The performance of the cell search when a mobile station is switched on, which is referred to as initial cell search, is decreased by the initial frequency and timing error. In this paper, we propose the pipeline structured initial cell search scheme using time trackers to compensate for the impact of the initial timing error in the stage 2 and stage 3. The simulation results show that the performance of the proposed scheme is maximal 1.5dB better than that of the conventional one when the initial timing error is near ${\pm}T_c$/2.

• Nuclear Engineering and Technology
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• v.22 no.1
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• pp.12-19
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• 1990

The Loss of Offsite Power Transient at 77.5% power which occurred on June 9, 1981 at the Kori Unit 1 PWR (Pressurized Water Reactor) is simulated using the RELAP5/MOD2 system thermal-hydraulics computer code. Major thermal-hydraulic parameters are compared with the available plant data. The comparison of the analysis results with the plant data demonstrates that the RELAP5/MOD2 code has the capability to simulate the thermal-hydraulic behaviour of PWRs under accident conditions of this type with accuracy, except the pressurizer pressure and level. The pressurizer pressure increase is sensitive to the in surge now it is believed that the interracial heat transfer in a horizontal stratified flow regime may be estimated low and the compression effect due to insurge flow may be high. In the nodalization sensitivity study it is found that S/G noding with junctions between bypass plenum and steam dome is preferred to simulate the S/G water level decreasing and avoid the spurious level peak at trubine trip.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05b
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• pp.513-518
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• 1996

This report presents a demonstration of application of realistic evaluation methodology to a posturated cold leg large break LOCA in a Westinghouse three-loop pressurized water reactor with 17$\times$17 fuel. The new method of this analysis can be divided into three distinct step: 1) Best Estimate Code Validation and Uncertainty Quantification 2) Realistic LOCA Calculation 3) Limiting Value LOCA Calculation and Uncertainty Combination RELAP5/MOD3/K ［1］, which was improved from RELAP5/MOD3.1, and CONTEMPT4/MOD5 code were used as a best estimate thermal-hydraulic model for realistic LOCA calculation. The code uncertainties which will be determined in step 1) were quantified already in previous study ［2］, and thus the step 2) and 3) for plant application were presented in this paper. The application uncertainty parameters are divided into two categories, i.e. plant system parameters and fuel statistical parameters. Single parameter sensitivity calculations were performed to select system parameters which would be set at their limiting value in Limiting Value Approach (LVA) calculation. Single run of LVA calculation generated 27 PCT data according to the various combinations of fuel parameters and these data provided input to response surface generation. The probability distribution function was generated from Monte Carlo sampling of a response surface and the upper 95$^{th}$ percentile PCT was determined. Break spectrum analysis was also made to determine the critical break size. The results show that sufficient LOCA margin can be obtained for the demonstration NPP.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.2
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• pp.35-42
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• 2021

In this study, a steel frame that realized the second floor of a structure was fabricated in referring to NFPA 13. In addition, a riser pipe system with groove joints was installed, and a seismic simulation test was performed using static cyclic loading. Cyclic loading tests on the maximum allowable side sway of seismic design standards for buildings in Korea were conducted using actuators to analyze the seismic behavior of the riser pipe system and major piping elements due to the deformation of the steel frame structure or the displacement-dominant behavior caused by the relative displacement between the structural members in the event of a seismic load. Moreover, the deformation angle of the riser pipe system was measured using an image measurement system because it is difficult to measure using the conventional sensors.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.14 no.2
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• pp.38-43
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• 2015

Tungsten inert gas (TIG) welding is commonly used in industries that require airtightness, watertightness, oiltightness, and precision. It is a non-consumable welding method that is commonly used for the welding of non-ferrous metals, but it can be used to weld most metals. The methods of TIG welding can be divided into three types. The first, manual welding is done directly on the metal by a welder with a torch. The second, semi-automatic welding, gets help from a material supplying machine, but it is conducted by a welder. Lastly, automated welding is conducted fully by a machine during its process and operation. Depending on the selection of electrode, the amount of heat that is applied to the base material and the electrode rod changes and makes the shape of welded parts different. A direct-current positive electrode was used for this study. Through the change of shielding gas type on a structural steel (SS-400) that is commonly used in industry, the composition and shape changes in welded parts were detected after welding. The heat-affected area, hardness value, and tensile strength were also identified through hardness testing and tensile testing. In this study, it was found that the higher hardness value of the heat-affected is, the weaker the tensile strength becomes.

• Journal of the Earthquake Engineering Society of Korea
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• v.21 no.2
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• pp.87-93
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• 2017

Many studies are conducted in several fields for fragility analysis of structures or elements which is a probabilistic seismic safety analysis in consideration with uncertainty of seismic loading. It is hard to directly conduct fragility analysis for an infrastructure with social importance due to its size. Therefore, a fragility analysis for an infrastructure mainly conducted in element level or conducted with scaled model built in accordance with similarity law. In this article, fragility analysis for prototype and scaled model of reinforced concrete column was conducted with numerical models which had been updated by the results of shaking table test and pseudo dynamic test. As a result, response stress from the numerical analysis result of prototype model was higher than that from scaled model due to different stiffness ratios between steel and concrete. However, the probability of failure for scaled model was higher than that for prototype model because failure criteria for scaled model was down due to similarity law. Also it was evaluated that probability of failure by using log normal standard deviation of response stresses by spectrum matched accelerograms was more reliable than probability of failure by using existing coefficient of variation normally used.