• Applied Microscopy
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• 제43권4호
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• pp.155-163
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• 2013

Meta-kaolin (MK) and blast furnace slag (BS) were used as raw materials with NaOH and sodium silicate as alkali activators for making geo-polymers. The compressive strength with respect to the various pre-curing conditions was investigated. In order to improve the recycling rate of BS while still obtaining high compressive strength of the geo-polymers, it was necessary to provide additional CaO to the MK by adding BS. The specimens containing greater amounts of BS can be applied to fields that require high initial compressive strength. Alkali activator(s) are inevitably required to make geo-polymers useful. High temperature pre-curing plays an important role in improving compressive strength in geo-polymers at the early stage of curing. On the other hand, long-term curing produced little to no positive effects and may have even worsened the compressive strength of the geo-polymers because of micro-structural defects through volume expansion by high temperature pre-curing. Therefore, a pre-curing process at a medium range temperature of $50^{\circ}C$ is recommended because a continuous increase in compressive strength during the entire curing period as well as good compressive strength at the early stages can be obtained.

• 한국산학기술학회논문지
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• 제20권9호
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• pp.510-516
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• 2019

본 연구는 염소 수정란의 체외배양용 배지에 FBS, gBS 및 PVA 첨가시 배발달 및 apoptosis 발생률을 분석하여 체외배양시 각 첨가물의 효과를 조사하였다. 체외성숙 후 체외수정을 실시하여 체외배양 배지에 10% FBS, 10% gBS 및 10% PVA를 첨가하고 배발달 효율과 배반포의 품질을 확인하기 위해 TUNEL assay를 통해 apoptosis 발생 비율을 조사한 결과, 난할률 및 배반포 형성률에서 처리군 모두 대조군(무혈청) 보다 유의적으로 높은 결과를 보였다. 특히 gBS와 PVA 처리군에서 각각 31.95, 35.29%로 유의적으로 가장 높은 배반포 형성률을 보였다. 또한 TUNEL assay의 결과에서도 배발달 비교실험에서와 같이 대조군에서 가장 적은 총세포수와 가장 높은 apoptosis 비율을 보였으며, gBS와 PVA 처리군에서 가장 많은 총세포수 및 가장 적은 apoptosis 비율을 보였다. 염소의 체외배양 효율 향상을 위한 본 연구의 결과, 무혈청 배지나 FBS첨가 보다는 gBS나 PVA의 첨가가 배발달 효율뿐만 아니라 배반포 품질에도 긍정적임을 알 수 있었으나, gBS내 확인되지 않는 물질들의 위험성을 감안했을 때 PVA가 보다 안전하고 효율적일 것으로 생각된다.

• 한국자원식물학회지
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• 제7권2호
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• pp.171-175
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• 1994

The effects of medium, hormones, and PFP on the proliferation of red callus in leaf tissue cultures of Garden orach(Atriplex hortensis L.) was investigated. As a result,88% of leaf tissues formed eallus on MS nledium containing 2mg/$\ell$ 2,4-D. Fresh weight of callus was higher on MS medium than on Bsand NN media. It was also found that 2, 4-D was more effective than Dicamba and Picloram. The op-timum concentrations of hormones for callus proliferation depended on culture media. Isolated red cal-lus grew markedly both on MS medium supplemented with 1-2mg/$\ell$ 2, 4-D and Bs medium contain-ing 2-4111g/$\ell$ 2,4D. Callus proliferated on B5 and NN media containing Dicabma Img/$\ell$ as well as onthe same media containing 2mg/$\ell$ Picioram. The addition of PFP concentrations of 2, 5, and 40mg/ $\ell$rcspectiely to culture medium caused increase of callus fresh weight, especially under light condition.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.491-497
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• 1998

Four types of serum supplements viz. estrus cow serum (ECS), estrus buffalo serum (EBS), pro-estrus buffalo serum (PrBS) and post-estrus buffalo serum (PtBS), added to TCM-199, were evaluated for in vitro maturation and fertilization of buffalo follicular oocytes. The oocytes were recovered from buffalo ovaries after slaughter, using either aspiration or scoring (multiple incisions) method. The recovered oocytes were categorized as A, B and C based on their cumulus investment and ooplasm homogeneity and cultured in four media. The in vitro matured oocytes were inseminated with $1{\times}10^6$ spermatozoa washed in 2.9% sodium citrate solution. The scoring method yielded greater number of morphologically good oocytes than the aspiration method (3.85 vs 1.76 per ovary, p < 0.01). The maturation rates of three categories of oocytes did not differ from one another. The maturation rates of 80.00, 82.08, 78.77 and 66.23%, while the fertilization rates of 54.54, 55.38, 52.80 and 36.76% were recorded for media containing ECS, EBS, PrBS, and PtBS, respectively. The medium containing PtBS gave lower maturation, as well as fertilization, rates than the other three media (p < 0.05). Thus, the scoring method was better than the aspiration method for the recovery of follicular oocytes. The oocytes categorized A, B and C had similar maturation capabilities. The TCM-199 containing buffalo/cow serum collected at pro-estrus or estrus appeared better for in vitro maturation and fertilization of buffalo follicular oocytes than that containing serum collected at post estrus.

• 한국해양바이오학회지
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• 제11권1호
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• pp.14-22
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• 2019

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

• 한국육종학회지
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• 제41권4호
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• pp.412-419
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• 2009

수박에서 종자크기의 유전분석을 위해 종자크기가 다른 6계통을 양친으로 한 교배집단을 조사하였다. 전체 6계통 중 3계통은 수집 계통으로 giant seed(GS)인 'PI525088' big size(BS)인 'Charleston Gray' 그리고 medium seed(NS)인 'NT'를 사용하였으며, 다른 3계통은 보통 크기와 가장 작은 크기 계통간($^{\prime}NT^{\prime}{\times}^{\prime}TDR^{\prime}$) 교잡 및 여교잡으로부터 육성되어 종자크기만 상이한 near isogenic line으로서 small seed(SS)인 'NTss' micro seed(MS)인 'NTms' tomato seed size(TS)인 'NTts'가 이용되었다. 각각의 종자크기에 관련한 유전양상을 파악하기 위해 인접한 종자크기를 가진 계통들간의 조합인 $GS{\times}BS$, $BS{\times}NS$, $NS{\times}SS$, $MS{\times}TS$, 그리고 종자크기가 비교적 차이가 계통들간 큰 두 조합인 $NS{\times}TS$, $GS{\times}NS$에 대하여 P1, P2, $F_1$, $F_2$, $BC_1F_1(P1)$, $BC_1F_1(P2)$를 작성, 전개하여 종자크기의 분리를 관찰하여 유전양식을 판단하였다. $GS{\times}BS$, $NS{\times}SS$, $MS{\times}TS$ 의 경우 1개의 유전자 차이가 발견되었고, 유전양식은 부분우성이었고, $BS{\times}NS$의 경우 2개 이상의 유전자가 관여하는 것으로 판단되며, $GS{\times}TS$에서는 분리후대에서 매우 넓은 범위의 종자크기를 가진 개체들이 관찰되어 이들 두 계통들간에는 6개 이상의 유전자, $NS{\times}TS$의 경우에는 3개의 유전자가 종자크기의 차이를 만드는 것으로 판단되었다.

• 한국산학기술학회논문지
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• 제20권9호
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• pp.333-340
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• 2019

본 연구는 염소 난자의 체외배양 시 혈청과 호르몬 첨가의 효과를 알아보기 위하여 체외성숙 효율, 유전자발현 및 체외수정 후 배발달율을 조사하였다. 본 실험에서는 기본 체외성숙 배양액에 10% gBS, 10% gFF를 첨가하여 혈청유무에 따른 효과와 FSH를 조합하여 체외성숙 효율을 조사한 결과, 처리구 모두 대조구보다 유의적으로 높은 성숙률을 보였다. 특히 gBS와 FSH를 조합하였을 때 처리구 중에서도 77.8%로 유의적으로 가장 높은 체외성숙 결과를 보였다. 또한, 각 처리구로부터 얻은 성숙 난자를 이용하여 체외수정 배발달 효율을 조사한 결과 모두 유의적으로 향상되었으며 특히 gBS와 gFF에 FSH를 혼합 처리한 두 그룹이 우수한 결과를 보였다. 난자의 성숙과 관련된 BMP15와 GDF9의 mRNA 발현량을 비교한 결과, 처리구간 특이점이나 유의적 차이가 나타나지 않았다. 본 연구의 결과 염소 난자의 체외성숙에 관한 추가적인 실험이 필요할 것으로 생각되나, gBS, gFF 및 FSH 첨가가 성숙 효율 향상에 도움이 됨을 알 수 있었다.

• 한국산학기술학회논문지
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• 제15권4호
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• pp.2134-2141
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• 2014

본 연구는 육상 100m 수평속도 요인에 기여하는 지면반력분석을 하기 위하여 여자 단거리 선수 8명을 대상으로 스타트 블록간의 거리를 세 가지(Bunch Start, Medium Start, Elongated Start)유형으로 8주간 출발에 관여하는 운동역학적 요인을 분석하였다. 최대 수평 지면반력은 단거리 경기 진행방향에 대한 추진력으로 전이되는 요인으로서 기록단축에 중요한 수치라고 볼 수 있다. 분석 결과는 앞쪽에 위치한 왼발에서는 우수그룹은 BS, 비우수그룹은 MS가 지면반발력 힘값이 가장 크게, 뒤쪽에 위치한 오른발에서는 우수, 비우수그룹 모두 MS에서 지면반발력 힘값이 가장 큰 결과를 보였다. 뒷발에 위치한 오른발은 우수그룹의 MS(0.83 BW)에서는 BS에서 추진력으로 전이될 때, 가장 유리한 출발 유형이라고 할 수 있다. 최대 수직 지면반력의 분석 결과는 앞쪽에 위치한 왼발에서는 우수그룹은 ES, 비우수그룹은 BS가 지면반발력 힘값이 가장 크게 나타났고, 뒷쪽에 위치한 오른발에서는 우수그룹은 BS, 비우수그룹은 MS에서 반발력 힘값이 가장 크게 나타났다. 지면 반발력이 크게 되면 초기 수평속도를 빠르게 얻을 수 있을 것으로 사료되며, 결국에는 전방 추진력을 크게 하는 자세나 방법을 찾게 되면 초기 기록의 향상을 가져올 수 있어 기록단축을 할 수 있다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권6호
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• pp.3012-3028
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• 2017

In this paper, we propose a joint power control strategy for both the uplink and downlink transmission by considering the energy requirements of the user equipments' uplink data transmissions in data and energy integrated communication networks (DEINs). In DEINs, the base station (BS) adopts the power splitting (PS) aided simultaneous wireless information and power transfer (SWIPT) technique in the downlink (DL) transmissions, while the user equipments (UEs) carry out their own uplink (UL) transmissions by exploiting the energy harvested during the BS's DL transmissions. In our DEIN model, there are M UEs served by the BS in order to fulfil both of their DL and UL transmissions. The orthogonal frequency division multiple access (OFDMA) technique is adopted for supporting the simultaneous transmissions of multiple UEs. Furthermore, a transmission frame is divided into N time slots in the medium access control (MAC) layer. The mathematical model is established for maximizing the sum-throughput of the UEs' DL transmissions and for ensuring their fairness during a single transmission frame T, respectively. In order to achieve these goals, in each transmission frame T, we optimally allocate the BS's power for each subcarrier and the PS factor for each UE during a specific time slot. The original optimisation problems are transformed into convex forms, which can be perfectly solved by convex optimisation theories. Our numerical results compare the optimal results by conceiving the objective of maximising the sum-throughput and those by conceiving the objective of maximising the fair-throughput. Furthermore, our numerical results also reveal the inherent tradeoff between the DL and the UL transmissions.

• 한국초지조사료학회지
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• 제15권1호
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• pp.1-12
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• 1995

This study was to investigate an effective biological control of forage diseases and provide a basic data and a model in improving variety of antagonistic bacteria, with growth promoting effect on forage, through cell fusion. The results obtained were summarized as follows; 1. The antagonistic himbacterium against soil-borne phathogenic fungi Fusarium oxysporum and Rhizoctonia solani was isolated from continuous cropping himsphere soil of forage, and its biological and physiological characteristics were investigated. This bacterium was identified as Bacillus subrilis and named BS 101. Another strain for cell fusion was Bacillus thur ingiensis ssp. kurstaki HD-I(BT 37669) with insecticidal crystal. 2. The auxotropic mutants of BS 101 and BT 37669 were derived after mutagenesis using N-methyl-N'nitro- Nitrosoguanidine(NTG) to give amino acid requirement marker. n e s e auxotropic mutants of BS 101 and BT 37669 were named BS 1013(his-) and BT 69(asp-), respectively. 3. The best protoplast requirement was obtained using DM 3 medium, containing 5% casamino acid, 1 M $MgCI_2$ and 2% bovine semm albumin, to give Fusant 3, 7 and 8. BT toxin gene was not identified with fusants by Southern blotting. However, SDS-PAGE analysis of strains showed various protein patterns among fusants. 4. From the dark culture experiment, growth of forage in inoculated soil with antagonistic bacteria was delayed than that of non-inoculated soil with antagonistic bacteria in each continuous cropping soil and in each sterilized soil. On the other hand, growth duration of forage was different between continuous cropping soil and sterilized soil. 5. Seed germination of Alfalfa, Italian ryegrass and Orchardgrass were significantly improved by inoculation of antagonistic bacteria(p< 0.05).