Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Influence of various serum supplement on in vitro culture for goat embryos

다양한 혈청 물질의 첨가가 염소 수정란의 체외배양에 미치는 영향

  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Jeon, Dayeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 전다연 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이성수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김승창 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김찬란 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2019.07.19
  • Accepted : 2019.09.06
  • Published : 2019.09.30
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Abstract

This study examined the effects of fetal bovine serum (FBS), goat blood serum (gBS), and poly-vinyl alcohol (PVA) on the in vitro development and embryo quality of goats for an improvement of embryo production. For the experiment, an in vitro fertilized embryo culture medium was supplemented with 10% FBS, 10% gBS, and 10% PVA to determine their effects on the embryo development efficiency and blastocyst quality. The results showed that the non-serum supplementation group showed significantly lower cleavage rate and blastocyst formation. On the other hand, the gBS and PVA supplementation groups showed a significant increase in the cleavage rate and better blastocyst formation than the control and FBS supplementation group. Furthermore, a TUNEL assay performed to confirm the blastocyst quality showed the same pattern as the embryo development experiment. These results showed that the supplemented gBS or PVA was more efficient in enhancing the in vitro development efficiency of goats than the supplementation of FBS or non-serum. On the other hand, considering the risk of an unidentified factor in gBS, PVA appears to be safer and more efficient in the in vitro development of goat embryos.

본 연구는 염소 수정란의 체외배양용 배지에 FBS, gBS 및 PVA 첨가시 배발달 및 apoptosis 발생률을 분석하여 체외배양시 각 첨가물의 효과를 조사하였다. 체외성숙 후 체외수정을 실시하여 체외배양 배지에 10% FBS, 10% gBS 및 10% PVA를 첨가하고 배발달 효율과 배반포의 품질을 확인하기 위해 TUNEL assay를 통해 apoptosis 발생 비율을 조사한 결과, 난할률 및 배반포 형성률에서 처리군 모두 대조군(무혈청) 보다 유의적으로 높은 결과를 보였다. 특히 gBS와 PVA 처리군에서 각각 31.95, 35.29%로 유의적으로 가장 높은 배반포 형성률을 보였다. 또한 TUNEL assay의 결과에서도 배발달 비교실험에서와 같이 대조군에서 가장 적은 총세포수와 가장 높은 apoptosis 비율을 보였으며, gBS와 PVA 처리군에서 가장 많은 총세포수 및 가장 적은 apoptosis 비율을 보였다. 염소의 체외배양 효율 향상을 위한 본 연구의 결과, 무혈청 배지나 FBS첨가 보다는 gBS나 PVA의 첨가가 배발달 효율뿐만 아니라 배반포 품질에도 긍정적임을 알 수 있었으나, gBS내 확인되지 않는 물질들의 위험성을 감안했을 때 PVA가 보다 안전하고 효율적일 것으로 생각된다.

Keywords

References

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