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http://dx.doi.org/10.5762/KAIS.2019.20.9.510

Influence of various serum supplement on in vitro culture for goat embryos  

Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Jeon, Dayeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.20, no.9, 2019 , pp. 510-516 More about this Journal
Abstract
This study examined the effects of fetal bovine serum (FBS), goat blood serum (gBS), and poly-vinyl alcohol (PVA) on the in vitro development and embryo quality of goats for an improvement of embryo production. For the experiment, an in vitro fertilized embryo culture medium was supplemented with 10% FBS, 10% gBS, and 10% PVA to determine their effects on the embryo development efficiency and blastocyst quality. The results showed that the non-serum supplementation group showed significantly lower cleavage rate and blastocyst formation. On the other hand, the gBS and PVA supplementation groups showed a significant increase in the cleavage rate and better blastocyst formation than the control and FBS supplementation group. Furthermore, a TUNEL assay performed to confirm the blastocyst quality showed the same pattern as the embryo development experiment. These results showed that the supplemented gBS or PVA was more efficient in enhancing the in vitro development efficiency of goats than the supplementation of FBS or non-serum. On the other hand, considering the risk of an unidentified factor in gBS, PVA appears to be safer and more efficient in the in vitro development of goat embryos.
Keywords
Serum; In Vitro Culture; Goat; Embryo; Blastocyst;
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