• 대한산업공학회지
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• 제41권5호
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• pp.488-498
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• 2015

Research on stem cells can be divided into three categories : pluripotent stem cell, adult stem cell, and induced pluripotent stem cell. Technology life cycle (TLC) on research stage is analyzed for the three stem cell categories based on diffusion model. Three diffusion models-logistic, Bass, and Bass model with integration constant (BMIC)-are applied to the number of articles related to each stem cell category in SCOPUS lists. Two different parameter estimation methods is used for each of logistic and Bass model. Results show that (1) the current year, 2015, lies in growth period at pluripotent stem cell and adult stem cell, and lies in growth period or maturity period at induced pluripotent stem cell. (2) Model fitness is the highest at BMIC model. (3) Imitation effect works best at the research area of induced pluripotent stem cell.

• 한국식품조리과학회지
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• 제24권2호
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• pp.222-227
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• 2008

The objective of this study was to evaluate the antioxidant activity of yuza chocolate,and to determine the optimum ratio of yuza powder for its preparation. Yuza peel and flesh powders were added to chocolate at weight percentages of 0, 3, and 6%, respectively. Color values (L-value, redness, and yellowness), total phenol content, total antioxidant capacity, DPPH radical scavenging activity, and sensory characteristics were then measured in the samples of yuza-containing chocolate. As the amount of yuza powder increased, total phenol content, antioxidant capacity, and radical scavenging activity increased. Depending on the level of yuza powder, significant differences (p <0.05) were shown in aroma, taste, bitterness, and overall acceptability however, there were no significant differences in texture. In terms of sensory acceptability, the addition of 3% yuza peel powder to chocolate was optimal.

• 공업화학
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• 제26권5호
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• pp.593-597
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• 2015

차세대 고밀도 연료인 tricyclopentadiene (TCPD)는 dicyclopentadiene (DCPD)를 Diels-Alder 소중합 반응을 통하여 제조하여 왔다. 이에 본 연구에서는 다양한 음이온 전구체와 양이온 전구체의 조합으로 만들어진 이온성 액체 촉매를 이용한 tricyclopentadiene (TCPD) 합성에 관한 연구를 수행하였다. 본 연구에 사용된 2가지 음이온 전구체는 copper(I) chloride (CuCl), iron(III) chloride ($FeCl_3$)이며 양이온 전구체는 triethylamine hydrochloride (TEAC), 1-butyl-3-methylimidazolium chloride (BMIC)이다. 이온성 액체 촉매의 소중합을 통한 TCPD의 제조는 기존 Diels-Alder 반응보다 DCPD의 전환율과 TCPD의 수율 측면에서 우수하였다. 또한, 음이온/양이온 전구체의 조합으로 제조된 이온성 액체 촉매의 산도와 TCPD 수율과의 상관관계가 있었다. 이온성 액체 촉매의 루이스 산도가 낮은 음이온 전구체로 CuCl를 이용하였을 때가 $FeCl_3$를 사용하였을 때보다 TCPD 수율이 좋았다. $FeCl_3$를 음이온 전구체로 하고 양이온 전구체로 BMIC를 사용하여 두 전구체의 몰 비를 조절하여 루이스 산도를 낮추면 TCPD 수율을 증가시킬 수 있었다.

• 대한지역사회영양학회지
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• 제10권4호
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• pp.453-461
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• 2005

This study was conducted to investigate the mothers' nutritional knowledge on weaning of 101 infants (8 for 4 mo, 44 for 5 mo, 45 for 6 mo, 4 for 7 mo) at a public health center of Incheon. Informations on the mothers' nutritional knowledge were obtained by questionnaires. In this survey $41.6\%$ of infants were breast-feeding, $43.6\%$ of them were bottle-feeding, and $14.9\%$ of them were mixed feeding right after birth. The rate of breast-feeding right after birth was significantly higher in mothers of high school graduate than mothers of college/university graduate (p < 0.05). The infants of mothers graduated high school began to be weaned significantly earlier than the infants of mothers graduated college/university (p < 0.05). $95.1\%$ of infants (n = 101) began to be weaned 4 to 6 months. $83.1\%$ of infants were fed home-made weaning foods. $66.7\%$ of infants were fed rice gruel, $18.5\%$ of them were fruit juice, $6.2\%$ of them were mixed grain, and $4.9\%$ of them were commercially prepared weaning foods as their first supplementary foods. As main supplementary foods, $32.8\%$ of infants were fed vegetable, $30.5\%$ of them were rice gruel, and $27.7\%$ of them were fruit juice. Mothers' nutritional knowledge related to weaning was significantly higher among mothers of college/ university graduate than mothers of high school graduate (p < 0.05). $86.1\%$ of mothers disagreed with the questionnaire in which breast-fed infants aged over 4 months needed to be fed iron sufficient food. For the improvement of nutritional status especially iron nutritional status of infants, nutritional education for mothers with weaning aged infants has to be increased and related programs have to be operated effectively.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.520-520
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• 2018

• Bulletin of the Korean Chemical Society
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• 제29권9호
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• pp.1797-1799
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• 2008

• 한국미생물·생명공학회지
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• 제37권2호
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• pp.99-104
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• 2009

Streptomyces coelicolor A3(2)의 $\beta$-glucosidase 유전자를 분리하여 대장균에서 발현하여 특성을 조사하였다. 최적 활성을 나타내는 온도는 pH 5에서는 $20^{\circ}C$, pH 6에서는 $60^{\circ}C$에서 높은 활성을 나타냈다. pH에 따른 활성은 pH 3 이하와 pH 9 이상의 범위에서는 낮은 활성을 나타냈으며 pH 7에서 가장 높은 활성을 나타냈다. $\alpha$-pNPG($\rho$-nitrophenyl-$\alpha$-D-glucopyranoside), $\beta$-pNPG ($\rho$-nitrophenyl-$\beta$-D-glucopyranoside), $\beta$-pNPF($\\rho$-nitrophenyl-$\beta$-D-fucopyranoside)는 pH 3-10까지 비슷한 활성을 나타냈으며, $\alpha$-pNPG가 pH 7에서 다소 높은 활성을 보였다. $\beta$-pNPGA는 pH 5-9까지 높은 활성을 나타냈으며, 특히 pH 9에서 3배 이상의 높은 활성을 나타냈다. 기질 $\alpha$-pNPG, $\beta$-pNPG, $\beta$-pNPF의 온도에 따른 활성변화는 $\beta$-pNPF의 활성이 $60^{\circ}C$에서 증가하였고, $\beta$-pNPGA는 $30-50^{\circ}C$까지 활성이 증가하여 $50^{\circ}C$에서 최대활성을 나타내었다. 당화 flavonoid를 이용한 기질특이성의 상대활성은 daidzin, glycitin, genistin, 순으로 나타났으며 esculin과 apigenin-7-glucose는 기질로 사용하지 않았다. $\beta$-Glucosidase 활성은 EDTA, DTT에 의해 억제되었으며, $MnSO_4$, $CaCl_2$, KCl, $MgSO_4$에 의해 증가하였고, 특히 Mn이온에 의해 증가하였다. $CuSO_4$, NaCl에 의해 효소활성이 저해되었으며, 특히 $ZnSO_4$의 경우 효소활성이 강하게 억제되었다.

• BMB Reports
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• 제53권2호
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• pp.88-93
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• 2020

Cisplatin is a widely used anti-cancer agent. However, the effectiveness of cisplatin has been limited by the commonly developed drug resistance. This study aimed to investigate the potential effects of endoplasmic reticulum (ER) stress to overcome drug resistance using the cisplatin-resistant A2780/CisR ovarian cancer cell model. The synthetic chalcone derivative (E)-3-(3,5-dimethoxyphenyl)-1-(2-methoxyphenyl)prop-2-en-1-one (named DPP23) is an ER stress inducer. We found that DPP23 triggered apoptosis in both parental cisplatin-sensitive A2780 and cisplatin-resistant A2780/CisR ovarian cancer cells due to activation of reactive oxygen species (ROS)-mediated unfolded protein response (UPR) pathway in the endoplasmic reticulum. This result suggests that ROS-mediated UPR activation is potential in overcoming drug resistance. DPP23 can be used as a target pharmacophore for the development of novel chemotherapeutic agents capable of overcoming drug resistance in cancer cells, particularly ovarian cancer cells.

• BMB Reports
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• 제44권7호
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• pp.452-457
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• 2011

The Egr-1 is an immediate early response gene encoding a transcription factor that functions in the regulation of cell growth, differentiation, and apoptosis. Estrogen has diverse physiological effects, including cellular proliferation and neuroprotection against brain injury. There are two types of estrogen receptors (ERs), $ER{\alpha}$ and $ER{\beta}$. $ER{\alpha}$-induced Egr-1 expression has been extensively studied; however, the role of $ER{\beta}$ is yet not known. In the present study, we investigated whether or not $ER{\beta}$ induces Egr-1 expression in C6 rat glioma cells, which express $ER{\beta}$ but not $ER{\alpha}$. Our results show that $ER{\beta}$ promoted up-regulation of Egr-1 expression via a non-genomic mechanism involving the Raf/MEK1/Erk/Elk-1 signaling cascade.

• Biomolecules & Therapeutics
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• 제27권2호
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• pp.210-215
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• 2019

Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.