• 제목/요약/키워드: BM

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Characterization of Low-Temperature Enzymatic Reactions through Heterologous Expression and Functional Analysis of Two Beta-Glucosidases from the Termite Symbiotic Bacterium Elizabethkingia miricola Strain BM10

  • Dongmin LEE;Tae-Jong KIM
    • Journal of the Korean Wood Science and Technology
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    • 제51권4호
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    • pp.270-282
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    • 2023
  • Lower termites need symbiotic microbes for cellulose digestion. Elizabethkingia miricola strain BM10 has been proposed as a symbiotic microbe that assists in low-temperature digestion and metabolism of Reticulitermes speratus KMT1, a termite on Bukhan Mountain, Seoul, Korea. In E. miricola strain BM10, β-glucosidase genes expressed at 10℃ were identified, and the psychrophilic enzymatic characteristic was confirmed by heterogeneously expressed proteins. Crude β-glucosidase in the culture broth of E. miricola strain BM10 showed specific enzymatic properties, and its substrate affinity was 4.69 times higher than that of Cellic CTec2. Among the genes proposed as β-glucosidase, two genes, bglB_1 and bglA_2, whose gene expression was more than doubled at 10℃ than at 30℃, were identified. They were heterogeneously expressed in Escherichia coli and identified as psychrophilic enzymes with an optimal reaction temperature of about 20℃-25℃. In this study, E. miricola strain BM10, a symbiotic bacterium of lower termites, produced psychrophilic β-glucosidases that contribute to the spread of the low-temperature habitat of a lower termite, R. speratus KMT1.

p38 mitogen-activated protein kinase contributes to TNFα-induced endothelial tube formation of bone-marrow-derived mesenchymal stem cells by activating the JAK/STAT/TIE2 signaling axis

  • Sukjin Ou;Tae Yoon Kim;Euitaek Jung;Soon Young Shin
    • BMB Reports
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    • 제57권5호
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    • pp.238-243
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    • 2024
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into endothelial cells in an inflammatory microenvironment. However, the regulatory mechanisms underlying this process are not entirely understood. Here, we found that TIE2 in BM-MSCs was upregulated at the transcriptional level after stimulation with tumor necrosis factor-alpha (TNFα), a major pro-inflammatory cytokine. Additionally, the STAT-binding sequence within the proximal region of TIE2 was necessary for TNFα-induced TIE2 promoter activation. TIE2 and STAT3 knockdown reduced TNFα-induced endothelial tube formation in BM-MSCs. Among the major TNFα-activated MAP kinases (ERK1/2, JNK1/2, and p38 MAPK) in BM-MSCs, only inhibition of the p38 kinase abrogated TNFα-induced TIE2 upregulation by inhibiting the JAK-STAT signaling pathway. These findings suggest that p38 MAP contributes to the endothelial differentiation of BM-MSCs by activating the JAK-STAT-TIE2 signaling axis in the inflammatory microenvironment.

트리플 밴드 초소형 전압 제어 발진기 (SMD형) 설계 및 제작 (Design and Fabrication of Triple Band Ultra-small VCO (SMD Type))

  • 조익현;김정삼;윤동한
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2003년도 하계종합학술대회 논문집 I
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    • pp.334-337
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    • 2003
  • GSM / DCS1800 / PCS 대역에서 사용 가능한 9.5mm x 6.5mm 크기의 트리플 밴드 초소형 전압제어 발진기를 설계하였다. 설계된 트리플 밴드 VCO는 GSM(880∼915MHz) 대역에서 7±3dBm의 출력전력, DCS1800 (1720∼1785MHz) 대역에서 8±3dBm의 출력전력, PCS(1720∼1780MHz) 대역에서 0±3dBm의 출력전력의 특성을 보인다. 위상 잡음의 특성은 -110dBc @100KHz 이하의 만족할만한 성능을 보인다.

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Fractal Interest Rate Model

  • Rhee, Joon-Hee;Kim, Yoon-Tae
    • 한국통계학회:학술대회논문집
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    • 한국통계학회 2005년도 춘계 학술발표회 논문집
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    • pp.179-184
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    • 2005
  • Empirical findings on interet rate dynamics imply that short rates show some long memories and non-Markovin. It is well-known that fractional Brownian motion(fBm) is a proper candidate for modelling this empirical phenomena. fBm, however, is not a semimartingale process. For this reason, it is very hard to apply such processes for asset price modelling. With some modifications, this paper investigate the fBm interest rate theory, and obtain a pure discount bond price and Greeks.

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중간가공열처리를 적용한 Al 7175 합금 형단조품의 기계적 성질

  • 손영일;이경훈;은일상;송영범;이용연
    • 한국추진공학회:학술대회논문집
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    • 한국추진공학회 1995년도 제5회 학술강연회논문집
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    • pp.181-191
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    • 1995
  • Al 7175 합금 형단조에서 주조조직 파괴를 위한 빌렛트제작(BM)공정과 ITMT공정 이 기계적 성질에 미치는 영향을 조사하였다. BM공정과 ITMT 공정을 동시 적용한 형단조품의 기계적 성질은 길이방향에서 인장강도가 10%, 연성이 50%, 충격흡수 에너지가 20% 증가되었고 횡방향에서도 인장강도가 7%, 연성이 9% 증가되었다. 이와 같은 기계적 성질의 향상은 BM과 ITMT공정을 동시 적용한 형단조품 미세조직의 결정립크기가 17$\mu\textrm{m}$로서 일반단조조직보다 3배이상 미세하고, 균일한 등축재결정 조직을 나타내는데 기인하는 것으로 판단된다.

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A Performance Consideration on Conversion Loss in the Integrated Single Balanced Diode Mixer

  • Han, Sok-Kyun;Kim, Kab-Ki
    • Journal of information and communication convergence engineering
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    • 제1권3호
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    • pp.139-142
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    • 2003
  • In this paper, we consider the factors that affect a conversion loss performance in designing a single balanced diode mixer integrated with IRF(Image Reject Filter), based on the embedded electrical wavelength placed between the IRF and mixer, diode matching and LO drive amplifier. To evaluate the conversion loss performance, we suggest two types of a single balanced mixer using 90 degree branch line coupler, microstrip line and schottky diode. One is only mixer and the other is integrated with IRF and LO drive amplifier. The measured results of a single balance diode mixer integrated IRF show the conversion loss of 8.5 dB and the flatness of 1 dB p-p from 21.2 GHz to 22.6 GHz with 10 dBm LO. The measured input PI dB and IIP3 are 7 dBm and 15 dBm respectively under the nominal LO power level of 10dBm. The LO/RF and LO/IF isolation are 22 dB and 50 dB, respectively.

전력증폭부의 광대역 특성 개선을 위한 최적의 부하 임피던스 설계 방안 연구 (A Study on Design of Optimal Load Impedance for Broadband Characteristic Improvement of the Power Amplifier)

  • 이한영
    • 전기학회논문지
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    • 제58권6호
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    • pp.1166-1173
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    • 2009
  • In this paper, we studied on the power amplifier which has the output of the optimal to 100MHz-2GHz band. Optimal output power match was fabricated using the two types; one is the linear tapering and the other is the impedance transformer. In the case of output power match using linear tapering, output power was 35.35dBm at 2GHz and 31.41dBm at 100MHz. The other case of output match using impedance transformer, output power was 34.8dBm at 2GHz and 33.25dBm at 100MHz. Comparison of the results in the two cases, impedance transformer type present the improved results by l.84dB of output power.

Development of Resistance to Bombyx mori Densonucleosis Virus into a Susceptible Silkworm Breed

  • Singh, G.P.;Xu, Mengkui;Chen, Yuyin;Datta, R.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제6권2호
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    • pp.145-149
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    • 2003
  • Seeing inadequate disinfection and unhygenic condition in rearing area, use of disease resistant silkworm variety is the best option. In order to this, an attempt has been made to develop the resistance to Bombyx mori densonucleosis virus (BmDNV-2) into a susceptible silkworm breed Zhenon1 by cross breeding with a resistant silkworm breed SU12 and exposing the subsequent generations to BmDNV-2 followed by the selection of individuals from the surviving batches. After seven generation the evolved DNV-2 resistant strain showed the significantly higher resistance to BmDNV-2 than control Zhenon1. The economic characters of both of the breeds were almost on par.