• Environmental Analysis Health and Toxicology
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• 제26권
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• pp.10.1-10.7
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• 2011

Objects: Chironomus riparius, a non-biting midge (Chironomidae, Diptera), is extensively used as a model organism in aquatic ecotoxicological studies, and considering the potential of C. riparius larvae as a bio-monitoring species, little is known about its genome sequences. This study reports the results of an Expressed Sequence Tags (ESTs) sequencing project conducted on C. riparius larvae using 454 pyrosequencing. Method: To gain a better understanding of C. riparius transcriptome, we generated ESTs database of C.ripairus using pyrosequencing method. Results: Sequencing runs, using normalized cDNA collections from fourth instar larvae, yielded 20,020 expressed sequence tags, which were assembled into 8,565 contigs and 11,455 singletons. Sequence analysis was performed by BlastX search against the National Center for Biotechnology Information (NCBI) nucleotide (nr) and uniprot protein database. Based on the gene ontology classifications, 24% (E-value${\leq}1^{-5}$) of the sequences had known gene functions, 24% had unknown functions and 52% of sequences did not match any known sequences in the existing database. Sequence comparison revealed 81% of the genes have homologous genes among other insects belonging to the order Diptera providing tools for comparative genome analyses. Targeted searches using these annotations identified genes associated with essential metabolic pathways, signaling pathways, detoxification of toxic metabolites and stress response genes of ecotoxicological interest. Conclusions: The results obtained from this study would eventually make ecotoxicogenomics possible in a truly environmentally relevant species, such as, C. riparius.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.30-30
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• 2014

We isolated various yeasts from wild flowers in main islands, Jejudo, Ulleungdo and Yokjido of Korea and their yeasts were identified by comparison of their PCR-amplified D1/D2 regions of 26S rDNA using the BLAST database. Thirty two yeast strains of fourteen species were isolated from wild flowers of Jejudo. Forty eight yeast strains of twenty two species were isolated and identified from wild flowers of Ulleungdo, Korea. Sixty yeast strains belonged to twenty five species were isolated identified from wild flowers of Yokjido in Tongyeong, Korea. Only Metschnikowia reukaufii was overlapped from the three different islands areas. Two species overlapped from Jejudo and Ulleungdo: Pichia guilliermondii, Metschnikowia reukaufii. Seven species were overlapped from Ulleungdo and Yokjido: Cryptococcus albidus, Cryptococcus laurentii, Metschnikowia reukafii, Pichia scolyti, Rhodotorula glutinis, Rhodotorula graminis and Rhodotorula mucilaginosa. Four species were overlapped from Jejudo and Yokjido: Candida sp. Cryptococcus aureus, Metschnikowia reukafii and Pseudozyma sp.

• 한국데이타베이스학회:학술대회논문집
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• 한국데이타베이스학회 1999년도 춘계공동학술대회: 지식경영과 지식공학
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• pp.261-270
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• 1999

Sintering transforms powdered ore into lumped ore so that the latter can be used in a blast furnace. The powdered ore combined with coke and other materials is loaded into a container and moved along by a pallet while the ignited coke bums. The speed by which the pallet moves determines how much sintering takes place. Since the process is complicated and lacks an accurate mathematical model, human operators manually control the speed by monitoring various factors in the plant. In this paper, we propose a neural network-based pallet speed controller which copies human operator knowledge. Actual process data were collected from a sintering plant fer eight months and preprocessed to remove noisy and inconsistent data. A multilayer perceptron was trained using a back-propagation learning algorithm. In on-line testing at the sinter plant, the proposed model reliably controlled pallet speed during normal operation without the help of human operators. Moreover, the duality and productivity was as good as with human operators.

• BMB Reports
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• 제40권2호
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• pp.286-289
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• 2007

Despite considerable interest in the biologic processes of regeneration and stem cell activation, little is known about the genes involved in these transformative events. In a Hydra littoralis model of regeneration, we employed a rapid shotgun suppression subtractive hybridization strategy to identify genes that are uniquely expressed in regenerating tissue. With an adaptor-PCR based technique, 16 candidate transcripts were identified, 15 were confirmed unique to mRNA isolated from hydra undergoing regeneration. Of these, 6 were undescribed in GenBank and allied expressed sequence tag (EST) databases (GenBank + EMBL + DDBJ + PDB and the Hydra EST database). BLAST analysis of these sequences identified remarkably similar sequences in anonymous ESTs found in a wide variety of animal species.

• 화약ㆍ발파
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• 제18권3호
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• pp.83-88
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• 2000

국내 지진관측소로부터 분석된 지진기록에는 자연지진 이외의 상당수의 인공발파를 포함하는 것으로 해석된다. 자연지진에 대한 지진특성연구, 지질학적 지진의 진원지연구 등을 위 해서는 지진목록에서 인공발파를 식별할 필요가 있다. 한국자원연구소는 인공발파 식별을 위한 연구의 일환으로 지진-공중음파 관측망을 운영 중에 있다. 지진-공중음파 자료분석으로 구분된 인공발파 기록의 대부분이 발파를 실시하는 산업현장과 일치하고 있음이 확인되었다. 발파장의 위치, 발파시간, 규모 및 발파방법 등의 정보는 공중음파를 이용한 인공발파 식별에 관한 정량적 연구와 자연지진에 관한 연구 등에 기본적인 정보(Ground Truth Database)를 제공하리라 판단되므로 국내에서 실시되는 인공발파에 대한 정보가 요구된다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2003년도 추계학술발표논문집 (하)
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• pp.1375-1378
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• 2003

본 논문은 Rice 60K DNA Chip의 실험데이터를 기반으로 한 데이터베이스의 구축과 XML기반 검색시스템을 설계 및 구현에 대해 설명한다. 본 시스템은 실험 데이터를 저장하기 위하여 RDBMS 를 사용하고 Chip 데이터를 검색하기 위해 XML 기반의 검색시스템을 사용한다. 이를 위해 일반 속성으로 저장될 수 있는 데이터들은 데이터베이스의 테이블의 속성 값으로 저장하고, XML 기반 검색시스템을 통해 검색할 수 있도록 한다. 그리고 BLAST내용을 기반으로 하는 데이터는 테이블을 별도로 만들어서 검색이 가능하도록 한다.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2003년도 춘계 학술발표회논문집
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• pp.38-44
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• 2003

Artificial blasts, defined as seismo-acoustic events, were discriminated from natural earthquakes in the Korean Peninsula by the seismo-acoustic analysis. Total 197 seismo-acoustic events corresponding to 9 percent of seismic events in 2002 were analyzed and classified as artificial surface blasts. Events distribution pattern of the seismo-acoustic events in 2002 was similar to the previous result in 1999-2001, except for two regions. Newly determined seismo-acoustic events were added to the previous artificial blast database. To extend infrasound detection capability, new small-scale infrasound array(TJIAR) was installed in KIGAM. Preliminary analysis for the small array was conducted to discriminate artificial blasts in the southwestern part of the Korean Peninsula. The small array discriminated S seismo-acoustic events during short period analysis. And two infrasound arrays(TJIAR and CHNAR) were used to determine approximate sound source location by cross bearing method.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1636-1650
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• 2007

As an initial step toward a better understanding of the genome structure of Korean cattle (Hanwoo breed) and initiation of the framework for genomic research in this bovine, the bacterial artificial chromosome (BAC) end sequencing of 21,024 clones was recently completed. Among these clones, BAC End Sequences (BESs) of 20,158 clones with high quality sequences (Phred score ${\geq}20$, average BES equaled 620 bp and totaled 23,585,814 bp), after editing sequencing results by eliminating vector sequences, were used initially to compare sequence homology with the known bovine chromosomal DNA sequence by using BLASTN analysis. Blast analysis of the BESs against the NCBI Genome database for Bos taurus (Build 2.1) indicated that the BESs from 13,201 clones matched bovine contig sequences with significant blast hits (E<$e^{-40}$), including 7,075 single-end hits and 6,126 paired-end hits. Finally, a total of 5,105 clones of the Korean cattle BAC clones with paired-end hits, including 4,053 clones from the primary analysis and 1,052 clones from the secondary analysis, were mapped to the bovine chromosome with very high accuracy.

• 한국건설순환자원학회논문집
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• 제5권4호
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• pp.488-495
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• 2017

본 연구에서는 열역학적 평형을 이용한 OPC의 재령에 따른 운동학적 수화 생성물 모델링과 GGBS의 치환률에 따른 수화 생성물의 변화를 모델링하였다. 열역학 데이터는 GEMS의 3rd party 데이터베이스, Cemdata18을 사용하였고, 시멘트 수화 모델링은 Parrot & Killoh 모델을 적용하였다. OPC모델링에서 재령에 따라 Pore solution의 이온 농도와 수화 생성물의 질량 및 부피 변화를 관찰할 수 있었다. GGBS모델링에서는 치환률이 증가함에 따라 장기강도를 지배하는 C-S-H의 생성량은 증가하지만, 내구성에 영향을 미치는 Portlandite의 생성량이 감소하여 탄산화에 대한 대책이 필요할 것으로 판단된다.

• 한국임상수의학회지
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• 제31권1호
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• pp.36-39
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• 2014

Many bacteria colonized in the horse semen affect quality of the sperm and some may cause infection in the mare reproductive tract and infertility of susceptible mare. This study was initiated to determine the prevalence of bacteria in testes of Jeju horses by determining rRNA sequence. The samples were swabed from the testes of nine Jeju horses (aged from 8 to 12 months after birth). Bacteria isolated from testes were identified by 16S rDNA sequencing. 1.6-kbp PCR products for 16S rRNA coding region were obtained using the universal primers. The PCR products were further purified and sequenced. Maximum similar species were found by BLAST search in the GenBank DNA database. BLAST results showed that the sequences were similar to those of Acinetobacter sp (A. schindleri, A. ursingii)., Bacillus cereus, Corynebacterium glutamicum, Escherichia coli, Gamma proteobacterium, Micrococcus luteus, Pseudomonas mendocina, Shigella sonnei, Sphingomonas sp., Staphylococcus sp (S. cohnii, S. saprophyticus, S. xylosus)., and Stenotrophomonas maltophilia. DNA sequences for 16S rRNA is provided useful informations for species identification of pathogenic microorganisms for the reproductive organs in horses.