Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Identification of Bacteria by Sequence Analysis of 16S rRNA in Testes of Jeju Horses

제주마 고환내 세균의 16S rRNA 염기서열 분석을 이용한 동정

  • 박용상 (농촌진흥청 국립축산과학원) ;
  • 김남영 (제주대학교 수의과대학) ;
  • 한상현 (제주대학교 교육과학연구소) ;
  • 박남건 (농촌진흥청 국립축산과학원) ;
  • 고문석 (농촌진흥청 국립축산과학원) ;
  • 조원모 (농촌진흥청 국립축산과학원) ;
  • 채현석 (농촌진흥청 국립축산과학원) ;
  • 조인철 (농촌진흥청 국립축산과학원) ;
  • 조상래 (농촌진흥청 국립축산과학원) ;
  • 우제훈 (농촌진흥청 국립축산과학원) ;
  • 강태영 (제주대학교 수의과대학)
  • Published : 2014.02.28
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Abstract

Many bacteria colonized in the horse semen affect quality of the sperm and some may cause infection in the mare reproductive tract and infertility of susceptible mare. This study was initiated to determine the prevalence of bacteria in testes of Jeju horses by determining rRNA sequence. The samples were swabed from the testes of nine Jeju horses (aged from 8 to 12 months after birth). Bacteria isolated from testes were identified by 16S rDNA sequencing. 1.6-kbp PCR products for 16S rRNA coding region were obtained using the universal primers. The PCR products were further purified and sequenced. Maximum similar species were found by BLAST search in the GenBank DNA database. BLAST results showed that the sequences were similar to those of Acinetobacter sp (A. schindleri, A. ursingii)., Bacillus cereus, Corynebacterium glutamicum, Escherichia coli, Gamma proteobacterium, Micrococcus luteus, Pseudomonas mendocina, Shigella sonnei, Sphingomonas sp., Staphylococcus sp (S. cohnii, S. saprophyticus, S. xylosus)., and Stenotrophomonas maltophilia. DNA sequences for 16S rRNA is provided useful informations for species identification of pathogenic microorganisms for the reproductive organs in horses.

Keywords

References

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