• 제목/요약/키워드: BC

검색결과 2,163건 처리시간 0.025초

정치배양을 이용하여 Acetobacter sp. V6의 셀룰로오스 생산 최적화 및 구조 분석 (Production and Structural Analysis of Cellulose by Acetobacter sp. V6 Using Static Culture)

  • 김정도;정호일;정진하;박기현;전영동;황대연;이충열;손홍주
    • 미생물학회지
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    • 제45권3호
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    • pp.275-280
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    • 2009
  • 한국 전통발효식초로부터 분리된 Acetobacter sp. V6에 의한 bacterial cellulose (BC) 생산을 위한 최적 배지조성을 정치배양을 이용하여 조사한 후, 생성된 BC의 구조적 특성을 검토하였다. 탄소원으로 glucose 3%, 질소원으로 soytone 3%가 선정되었다. 또한 인산염은 $K_2HPO_4$ 0.8%였으며, 추가로 첨가한 $NaH_2PO_4$$KH_2PO_4$는 BC 생산 증가를 나타내지 못하였다. 보조탄소원으로 ethanol 0.4%에서 가장 높은 BC 생산성을 나타내었다. 최적 배지조성하에서 배양 8일만에 최대 44.7 g/$m^2$의 BC가 생산되었으며, 배양 8일경, BC pellicle의 두께는 약 1 cm였다. BC의 구조는 Fourier-transform infrared spectroscopy 및 X-ray diffractometer를 이용하여 조사하였다. 최적배지에서 생성된 BC는 전형적인 cellulose type I임을 알 수 있었으며, 식물성 셀룰로오스와 성분의 차이가 없었다. 또한 독특한 미세망상구조로 이루어져 있었고, 높은 결정성을 나타내어 식물유래 셀룰로오스와 다른 독특한 물성을 나타내는 것으로 추정되었다.

Pilot Scale의 박테리아 셀룰로오스 생산 및 그의 물성 (Production of Bacterial Cellulose by Pilot Scale and Its Properties)

  • 김성준;송효정;장미화;최창남
    • KSBB Journal
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    • 제22권2호
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    • pp.91-96
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    • 2007
  • 본 연구에서는 음식폐기물 효소당화액을 BC생산 배지원으로 이용하여, flask정치배양과 50 L로 규모확대한 공기순환배양기에서 BC생산성을 검토하고, 생산된 BC의 제반물성을 조사하였다. 전단력을 낮추기 위해 당화액배지에 agar를 첨가하여 10 L, 50 L 공기순환배양기에서 배양한 결과, 50 L 규모로 scale-up한 반응기에서 5.64 g/L로 10 L 공기순환배양기 (5.84 g/L)에서와 동등한 BC 농도가 얻어져 BC 대량생산에 효과적인 배양방법임이 입증되었다. 50 L 규모로 확대된 공기순환배양기를 이용하는 본 배양 방법은 BC의 저비용 대량생산을 위한 효율적인 생산 방법 중 하나가 되리라 사료된다. 또한, 당화액을 배지로 하여 공기순환 방법으로 제조한 BC의 물성을 정치배양 방법으로 제조한 BC의 물성과 비교 검토한 결과, 공기순환법으로 제조하였을 때 중합도 및 역학적 성질이 약간 저하하였으나 결정성은 비슷하였으며, 배양기 용량의 대소에 따른 물성의 변화는 거의 없었다.

Effect of Lactate and Corn Steep Liquor on the Production of Bacterial Cellulose by Gluconacetobacter persimmonis $KJ145^T$

  • Jang, Se-Young;Jeong, Yong-Jin
    • Food Science and Biotechnology
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    • 제14권5호
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    • pp.561-565
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    • 2005
  • In this study, we attempted to assess the effects of lactate and com steep liquor (CSL) on the production of bacterial cellulose (BC) by Gluconacetobacter persimmonis $KJ145^T$. The optimal condition for the production of BC was a lactate concentration of 1% (w/v) and a CSL concentration of 10% (w/v). Under these optimal conditions, 6 days of fermentation produced 6.90 g/L of BC. Both the BC production yield and cell growth increased continuously until the 20th day of fermentation, by which time 17.0 g/L had been produced. In a static culture trial, in which plastic containers were used as fermentation chambers for 6 days of fermentation, the BC production yield in the group initially cultured with 500 mL medium was higher than that of the 750 and 1000 mL media. In addition, the texture of the BC was examined according to its post-treatment in order to determine conditions for optimal textural characteristics. The strength, hardness, and other characteristics of the BC were negatively correlated with sucrose concentration, but were largely positively correlated with NaCl concentration. With regards to the effect of pH on textural change, BC strength and hardness were elevated at pH 2 and 8 but reduced at pH 4 and 6, indicating that the texture of the BC is extremely sensitive to treatment conditions.

차세대 mBcN을 위한 5G+ 연동보안게이트웨이 (A Cooperative Security Gateway cooperating with 5G+ network for next generation mBcN)

  • 남구민;김형식;이현진;조학수
    • 인터넷정보학회논문지
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    • 제22권6호
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    • pp.129-140
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    • 2021
  • 차기 mBcN에서는 초고속·초연결을 지원할 수 있는 무선망과 연계할 수 있도록 구축되어야 한다. 본 논문에서는 5G 무선망과 mBcN의 연계하기 위한 연동망 구조와 연동에 필요한 연동보안게이트웨이의 구조를 제안한다. 제안하는 연동보안게이트웨이는 gNB와 UPF의 사이에 위치하며 LBO 기능, SFC 기능 및 다양한 보안 기능을 제공한다. 제안하는 연동망 구조 및 연동보안게이트웨이를 통해 다양한 이점을 확보할 수 있다. 첫 번째는 mBcN망과 연결이 필요한 사용자 단말은 5G망의 무선망을 활용하여 mBcN망과 연결할 수 있다. 두 번째는 mBcN으로 전달되는 트래픽은 5G 코어망을 경유하지 않고 mBcN으로 전송되어 5G 코어망에 망부하를 야기하지 않으면서 종단간 전송 지연을 감소시킬 수 있다. 마지막으로 군응용체계 패킷이 5G코어망으로 전파되지 않고 연동 보안게이트웨이와 연결된 기지국을 통해서만 mBcN과 연결할 수 있어 보안을 유지할 수 있다. 마지막으로 본 논문에서는 5G 테스트배드 환경에서 실험을 통해 제안하는 연동 보안 게이트웨이의 LBO 기능, SFC 기능 및 보안모듈의 기능을 제공 가능함을 제시한다.

Streptozotocin으로 유발된 당뇨쥐에 대한 백강잠(白?蠶)의 혈당 및 당대사 효소활성에 관한 효과 (Effects of Bombycis corpus on Streptozotocin-induced Diabetic Rats)

  • 현민경;신원용;김미랑;신현철;윤철호;정지천;정병무
    • 대한한방내과학회지
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    • 제25권2호
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    • pp.288-297
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    • 2004
  • Objective : This study was undertaken to investigate how Bombycis corpus (BC) effects the development and progress of complications occurring in Diabetes Mellitus (DM). Methods : Laboratory rats were seperated into three groups; normal, rats with DM and treated with BC, and rats with DM and not treated. In this study DM was experimentally induced through injection of streptozotocin. The BC treated group was given BC extract p.o. for 15 days. Then, The activities of glucose phosphatic enzymes and polyol pathway channels were observed. Results : The blood glucose level greatly increased in the DM groups after injection of streptoztocin, but it significantly decreased in the BC treated group. Significantly enhanced levels of serum insulin levels were seen in the BC treated group, while supressed levels were seen in the untreated DM group. Weight was recovered by the BC treated group, matching the normal group. Decreased enzyme activity of aldose reductase, sorbitol dehydrogenase and glucose-6-phosphatase were seen in BC treated diabetic rats. Increased enzyme activity, of the glucokinase and hexokinase were seen in BC treated diabetic rats. Conclusions : This study suggests that BC normalized the blood glucose and serum insulin levels destablized by DM. Because increased activity of glucose phosphatic enzymes, glucokinase and hexokinase, and decreased glucose-6-phosphatase activity, and suppression of polyol pathway enzymes, aldose reductase and sorbitol dehydrogenase, were all seen, these observations suggest that BC suppresses blood glucose levels and prevents complications due to DM.

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Ethylene Vinyl Acetate / Ethylene-1-Butene Copolymer 블렌드 발포체의 제조와 특성 (Preparation and Properties of Ethylene Vinyl Acetate/Ethylene-1-Butene Copolymer Blend Based Foam)

  • 차길수;김진태;윤정식;김원호
    • Elastomers and Composites
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    • 제36권1호
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    • pp.14-21
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    • 2001
  • 신발 중창용 소재인 ethylene vinyl acetate (EVA) 발포체의 인장강도, 반발탄성, 영구압축줄음율 (compression set) 등의 물성을 향상시킬 목적으로 ethylene-1-butene copolymer (EtBC)를 EVA에 블렌드하여 가교특성을 조사하였으며, 발포체를 제조한 후 셀의 구조적 특성 및 발포체의 기계적 물성을 조사하였다. EVA/EtBC 블렌드에서, EtBC의 함량이 증가할수록 블렌드의 점도 및 가교 밀도는 증가하여 oscillating disk rheometer (ODR)에서 높은 torque 값을 나타내었으며 발포배율은 감소하였다. 발포제 함량의 증가에 따라서 발포배율 및 셀의 크기는 증가하였다. 발포체를 동일 비중에서 비교하였을 경우, EtBC 함량이 증가할수록 EVA/EtBC 발포체의 인장강도, 영구압축줄음율, 반발탄성 등 기계적 물성이 우수해졌다.

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KIAPS 자료동화 시스템에서 AMSU-A의 품질검사 및 편향보정 반복기법에 관한 연구 (A Study of Iterative QC-BC Method for AMSU-A in the KIAPS Data Assimilation System)

  • 정한별;전형욱;이시혜
    • 대기
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    • 제29권3호
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    • pp.241-255
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    • 2019
  • Bias correction (BC) and quality control (QC) are essential steps for the proper use of satellite observations in data assimilation (DA) system. BC should be calculated over quality controlled observation. And also QC should be performed for bias corrected observation. In the Korea Institute of Atmospheric Prediction Systems (KIAPS) Package for Observation Processing (KPOP), we adopted an adaptive BC method that calculates the BC coefficients with background at the analysis time rather than using static BC coefficients. In this study, we have developed an iterative QC-BC method for Advanced Microwave Sounding Unit-A (AMSU-A) to reduce the negative feedback from the interaction between BC and QC. The new iterative QC-BC is evaluated in the KIAPS 3-dimensional variational (3DVAR) DA cycle for January 2016. The iterative QC-BC method for AMSU-A shows globally significant benefits for error reduction of the temperature. The positive impacts for the temperature were predominant at latitudes of $30^{\circ}{\sim}90^{\circ}$ of both hemispheres. Moreover, the background warm bias across the troposphere is decreased. Even though AMSU-A is mainly designed for atmospheric temperature sounding, the improvement of AMSU-A pre-processing module has a positive impact on the wind component over latitudes of $30^{\circ}S$ near upper-troposphere, respectively. Consequently, the 3-day-forecast-accuracy is improved about 1% for temperature and zonal wind in the troposphere.

Anti-apoptotic effect by Bcl-2 in UVB-irradiated keratinocytes.

  • Takahashi, Hidetoshi;Honma, Masaru;Ishida-Yamamoto, Akemi;Namikawa, Kazuhiko;Miwa, Akiko;Okado, Haruo;Kiyama, Hiroshi;Iizuka, Hajime
    • Journal of Photoscience
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    • 제9권2호
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    • pp.225-228
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    • 2002
  • Bcl-2 is a member of large bcl-2 family and protects cells from apoptosis. Using bcl-2-expressing adenovirus vector (Ad-bc1-2), we investigated the effect of bc1-2 on UVB-induced apoptosis. Adenovirus vector efficiently introduced bc1-2 gene in cultured normal mouse keratinocytes (NMK cells); almost all NMK cells (lx10$^{6}$ ) were transfected at Ixl0$^{8}$ PFU/ml. Bcl-2-transfected NMK cells were significantly resistant to UVB-induced apoptosis with the suppressive effect dependent on bcl-2-expression level. Following UVB irradiation caspase 8, 3, 9 activities were stimulated in NMK cells, while in bc1-2-transfected cells, only caspase 8, but not caspase 3 or 9 activities were stimulated. In order to investigate the effect of bc1-2 in vivo, topical application of Ad-bc1-2 on tape-stripped mouse skin was performed. Following the application, Bc1-2 was efficiently overexpressed in almost all viable keratinocytes. The expression was transient with the maximal expression of Bc1-2 at 1st day following the application of lxl0$^{9}$ PFU in 200ml. The introduced Bc1-2 remained at least for 6 days. UVB irradiation (1250 J/m$^2$) induced apoptosis within 12 h and the maximal effect was observed at 24 h in control mouse skin. Bc1-2-transfected mice skin were resistant to UVB-induced apoptosis. Topical application of empty adenovirus vector alone had no effect on Bc1-2 expression or UVB-induced apoptosis. These results indicate that adenovirus vector is an efficient gene delivery system into keratinocytes and that Bcl-2 is a potent inhibitor of UVB-induced apoptosis both in vitro and in vivo.

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BcN 적합형 액세스네트워크 구조 (The Access Network Architecture for BcN Adapted)

  • 이상문
    • 한국정보통신설비학회:학술대회논문집
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    • 한국정보통신설비학회 2007년도 학술대회
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    • pp.121-124
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    • 2007
  • This article describes a function and structure of access network equipment under BcN environment. Access network until now have constructed separately to offer voice, data service. However, simplifies network structure, function that can do traffic concentration, subscriber certification, individual charging, QoS according to service and routing is required in BcN. In this paper, compare method offering by separate system with existing access network and method that offer integrating function inside system for structure of suitable access network to BcN and search structure of access network equipment for desirable access network of hereafter. Composition of this paper is as following. In Chapter 2, establishment history and structure of access network until present. In Chaprte 3, define suitable requirement and functions to BcN. And compare structure for access net work that is new with present. Last Chapter 4, suggests direction of structure of BcN access network and concludes conclusion.

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사용자 중심의 광대역통합망 서비스 품질척도 연구 (Developing End-User Oriented Quality Dimensions for BcN Service)

  • 은현지;김성철
    • 경영과학
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    • 제23권3호
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    • pp.119-132
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    • 2006
  • Since the guaranteed service quality(QoS) is spotlighted as a strategic competitive tool to Broadband Convergence Network(BcN) service providers, many scholars have dealt with QoS issues in the BcN environment. However, just a few researchers focus on end-users and most are lean toward service providers or technology itself. In this paper, therefore, we investigated how end-users could measure service quality in the BcN environment, and moreover, proposed the end-user oriented measurement guideline for BcN QoS. Through literature review and two surveys, four QoS dimensions and nineteen measurement items were derived from end-user's perspective. proposed QoS dimension and items would be utilized as meaningful tools for future BcN service.