Browse > Article

Production and Structural Analysis of Cellulose by Acetobacter sp. V6 Using Static Culture  

Kim, Jeong-Do (College of Natural Resources and Life Science, Pusan National University)
Jung, Ho-Il (College of Natural Resources and Life Science, Pusan National University)
Jeong, Jin-Ha (College of Natural Resources and Life Science, Pusan National University)
Park, Ki-Hyun (College of Natural Resources and Life Science, Pusan National University)
Jeon, Young-Dong (College of Natural Resources and Life Science, Pusan National University)
Hwang, Dae-Youn (College of Natural Resources and Life Science, Pusan National University)
Lee, Chung-Yeol (College of Natural Resources and Life Science, Pusan National University)
Son, Hong-Joo (College of Natural Resources and Life Science, Pusan National University)
Publication Information
Korean Journal of Microbiology / v.45, no.3, 2009 , pp. 275-280 More about this Journal
Abstract
The optimal medium compositions for the production of bacterial cellulose (BC) by a Acetobacter sp. V6, which was isolated from the traditionally fermented vinegar in Korea, were investigated in static cultures. The optimum medium compositions for BC production were 3% glucose, 3% soytone, 0.8% $K_2HPO_4$, and 0.4% ethanol, respectively. Adding $NaH_2PO_4$ or $KH_2PO_4$ had not shown the increase in BC production. Under the optimum medium compositions, the highest BC production was 44.67 g/$m^2$ in 8 days and the thickness of BC pellicle was about 1 cm. Structural properties of BC produced in the optimal medium were studied using Fourier-transform infrared spectroscopy and X-ray diffractometer. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose. No difference in the compositions between bacterial and plant celluloses, but BC showed unique micro-network structure and high crystallinity (82%).
Keywords
Acetobacter sp.; bacterial cellulose; cellulose type I; static culture;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Dudman, W.F. 1959. Cellulose production by Acetobacter acetigenum and other Acetobacter spp. J. Gen. Microbiol. 21, 312-326   DOI   PUBMED   ScienceOn
2 Focher, B., M.T. Palma, M. Canetti, G. Torri, C. Cosentino, and G. Gastaldi. 2001. Structural differences between non-wood plant celluloses: evidence from solid state NMR, vibrational spectroscopy and X-ray diffractometry. Ind. Crops Prod. 13, 193-208   DOI   ScienceOn
3 Klemm, D., D. Schumann, U. Udhard, and S. Marsch. 2001. Bacterial synthesized cellulose - artficial blood vessels for microsurgery. Prog. Polym. Sci. 26, 1561-1603   DOI   ScienceOn
4 Paek, H.S., J.S. Park, S.M. Jo, W.S. Lee, and K.J. Kim. 1993. The effects of preparation conditions of celluose/N-methylmorpholine-N-oxide solutions on physical properties of fibers. J. Korean Fiber Soc. 30, 569-577   과학기술학회마을
5 Sutherland, I.W. 1998. Novel and estabilished applications of microbial polysaccharides. Tibtech. 16, 41-46   DOI   ScienceOn
6 Williams, W.S. and R.E. Cannon. 1989. Alternative environmental roles for cellulose produced by Acetobacter xylinum. Appl. Environ. Microbiol. 55, 2448-2452   PUBMED   ScienceOn
7 Yamanaka, S., K. Watanabe, N. Kitamura, M. Iguchi, S. Mitsuhashi, Y. Nishi, and M. Uryu. 1989. The structure and mechanical properties of sheets prepared from bacterial cellulose. J. Mat. Sci. 24, 3141-3145   DOI
8 Sakran, M.A. 1996. Mechanism of cellulose polymer reactions with some cation-exchanged montmorillonite catalysts. J. Radioanal. Nucl. Chem. 213, 87-98   DOI   ScienceOn
9 Son, H.J., O.M. Lee, Y.G. Kim, Y.K. Park, and S.J. Lee. 2000. Characteristics of cellulose production by Acetobacter sp. A9 in static culture. Korean J. Biotechnol. Bioeng. 15, 573-577   과학기술학회마을   ScienceOn
10 Oikawa T., T. Ohtoti, and M. Ameyama. 1995. Production of cellulose from D-mannitol by Acetobacter xylinum KU-1. Biosci. Biotech. Biochem. 59, 331-332   DOI
11 Toda, K., T. Asakura, M. Fukaya, E. Entani, and Y. Kawamura. 1997. Cellulose production by acetic acid-resistant Acetobacter xylinum. J. Ferment. Bioeng. 84, 228-231   DOI   ScienceOn
12 Fontana, J.D., A.M. De Souza, C.K. Fontana, I.L. Torriani, J.C. Moreschi, B.J. Gallotti, S.J. De Souza, G.P. Narcisoo, J.A. Bichara, and L.F.X. Farah. 1990. Acetobacter cellulose pellicle as a temporary skin substitute. Appl. Biochem. Biotechnol. 24/25, 253-264   DOI   ScienceOn
13 Embuscado, M.E., J.N. BeMiller, and J.S. Marks. 1996. Isolation and partial characterization of cellulose produced by Acetobacter xylinum. Food Hydrocoll. 10, 75-82   DOI   ScienceOn
14 Ko, J.Y., K.S. Shin, B.D. Yoon, and W.Y. Choi. 2002. Production of baterial cellulose by Axetobacter xylinum GS11. Kor. J. Appl. Microbiol. Biotechnol. 30, 57-62   과학기술학회마을
15 Yamanaka, S. and K. Watanabe. 1998. Applications of bacterial cellulose in cellulosic polymers, pp. 207-215. In R. Gillbert (ed.), Cellulosic polymers - Blends and composites, Hanser Inc., Cincinnati, OH, USA
16 Naritomi, T., T. Kouda, H. Yano, and F. Yoshinaga. 1998. Effect of ethanol on bacterial cellulose production from fructose in continuous culture. J. Ferment. Bioeng. 85, 598-603   DOI   ScienceOn
17 Byrom, D. 1991. Microbiol cellulose, pp. 263-284. In D. Byrom (ed.), Biomaterials, Stockton Press, New York, N.Y., USA
18 Hestrin, S. and M. Schramm. 1954. Synthesis of cellulose by Acetobacter xylinum. Biochem. J. 58, 345-352   DOI   PUBMED   ScienceOn
19 Son, H.J., O.M. Lee, Y.G. Kim, and S.J. Lee. 2000. Isolation and identification of cellulose-producing bacteria. Kor. J. Appl. Microbiol. Biotechnol. 28, 134-138   과학기술학회마을
20 Cannon, R.E. and S.M. Anderson. 1991. Biogenesis of bacterial cellulose. Crit. Rev. Microbiol. 17, 435-447   DOI   ScienceOn
21 Ross, P., Y. Aloni, H. Weinhouse, D. Michaeli, P. Weinberger-Ohana. R. Mayer, and M. Benziman. 1986. Control of cellulose synthesis in Acetobacter xylinum. A unique guanyl oligonucleotide is the immediate activator of the cellulose synthase. Carbohyd. Res. 149, 101-117   DOI   ScienceOn
22 Czaja, W.K., D.J. Young, M. Kawecki, and R.M. Brown, Jr. 2007. The future prospects of microbial cellulose in biomedical applications. Biomacromol. 8, 1-12   DOI   ScienceOn
23 Rainer, J. and F.F. Luiz. 1986. Production and application of microbial cellulose. Polym. Degrad. Stab. 58, 101-106
24 Ross, P., H. Weinhouse, Y. Aloni, D. Michaeli, P. Weinberger- Ohana, R. Mayer, S. Braun, E. de Vroom, G.A. van der Marel, J.H. van Boom, and M. Benziman. 1987. Regulation of cellulose synthesis in Acetobacter xylinum by cyclic diguanylic acid. Nature 325, 279-281   DOI   PUBMED
25 Valla, S. and J. Kjosbakken. 1982. Cellulose negative mutants of Acetobacter xylinum. J. Gen. Microbiol. 128, 1401-1408   DOI
26 Matsuoka, M., T. Tsuchida, K. Matsushita, O. Adachi, and F. Yoshinaga. 1996. A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp. sucrofermentans. Biosci. Biotech. Biochem. 60, 575-579   DOI   ScienceOn